MOROHAKU Kanako

Academic Assembly School of Science and Technology Institute of Agriculture

Faculty of Agriculture Department of Agricultural and Life Sciences Division of Animal Science 

Assistant Professor 

Degree

  • Ph.D., Tohoku University

Research Keyword

    in vitro oocyte growth, folliculogenesis, oogenesis

Field Of Study

  • Animal reproduction

Career

  • 2017
    Shinshu University, Assistant Professor
  • 2013 - 2017
    Tokyo University of Agriculture, Postdoctoral Associate
  • 2011 - 2013
    Postdoctoral Associate, Cornell University
  • 2010 - 2011
    JSPS Research Fellow (PD)
  • 2009 - 2010
    JSPS Research Fellow (DC2)

Educational Background

  • 2007, Tohoku University, 農学研究科, 応用生命科学専攻 博士課程前期2年の課程
  • 2010, Tohoku University, 農学研究科, 応用生命科学専攻 博士課程後期3年の課程
  • 2005, Tohoku University, 農学部, 生物生産科学科
Research activity information

Award

  • 2023
    The Society for Reproduction and Development, Journal of Reproduction and Development Outstanding Paper Award 2022
  • 2018
    Society for Reproduction and Development, SRD Young Investigator Award 2018
  • 2017
    Proceedings of National Academy of Sciences of the United States of America, Cozzarelli Prize 2016
  • 2014
    Endocrine Society, Endocrine Society, The Best of Basic Research 2014

Paper

  • Mitochondrial translocator protein (TSPO) deficiency exacerbates pathology in acute experimental ulcerative colitis
    Jimenez I, Stilin A, Morohaku K, Hussein M, Koganti P, Selvaraj V.
    Frontiers Physiology, Aug. 2022, Refereed
  • In vitro production of viable eggs from mouse isolated primary follicles by successive culture
    Kohama T, Masago M, Tomioka I, Morohaku K.
    Journal of Reproduction and Development, 68(1), 38-44, Feb. 2022, Refereed
    Corresponding
  • In vitro production of viable eggs from isolated mouse primary follicles by successive culture
    Kohama, Tomohiro; Masago, Maika; Tomioka, Ikuo; Morohaku, Kanako;
    Journal of Reproduction and Development, 68(1), 38-44, 2022WebofScience電子ジャーナル
  • Blocking estrogen-induced AMH expression is crucial for normal follicle formation
    Tanimoto, R. Sekii, K. Morohaku, K. Li, J. Pépin, D. Obata, Y.
    DEVELOPMENT, 148(6), Mar. 2021, Refereed電子ジャーナル
  • Blocking estrogen-induced AMH expression is crucial for normal follicle formation
    Tanimoto, Ren; Sekii, Kiyono; Morohaku, Kanako; Li, Jianzhen; Pepin, David; Obata, Yayoi;
    DEVELOPMENT, 148(6), dev197459, Mar. 2021WebofScience電子ジャーナル
  • Commentary:Amhr2-Cre-Mediated Global TSPO Knockout
    Vimal Selvaraj, Kanako Morohaku, Prasanthi P. Koganti, Jianmin Zhang, Wei He, Susan M. Qurik, Douglas M. Stocco
    FRONTIERS IN ENDOCRINOLOGY, Volume 11, 24 Jul. 2020, Refereed
  • Commentary: Amhr2-Cre-Mediated Global Tspo Knockout
    Selvaraj, Vimal; Morohaku, Kanako; Koganti, Prasanthi P.; Zhang, Jianmin; He, Wei; Quirk, Susan M.; Stocco, Douglas M.;
    FRONTIERS IN ENDOCRINOLOGY, 11, 472, 2020WebofScience電子ジャーナル
  • A way for in vitro/ex vivo egg production in mammals
    Kanako Morohaku
    Journal of Reproduction and Development, 65(4), 281-287, Aug. 2019, Refereed
  • A way for in vitro/ex vivo egg production in mammals
    Morohaku, Kanako;
    Journal of Reproduction and Development, 65(4), 281-287, Aug. 2019WebofScience
  • Ectopic expression of DNA methyltransferases DNMT3A2 and DNMT3L leads to aberrant hypermethylation and postnatal lethality in mice
    K. Sasaki, S. Hara, R. Yamakami, Y. Sato, S. Hasegawa, T. Kono, K. Morohaku, Y. Obata
    Molecular Reproduction and Development, 86, 614-623, Jun. 2019, Refereed
  • Ectopic expression of DNA methyltransferases DNMT3A2 and DNMT3L leads to aberrant hypermethylation and postnatal lethality in mice
    Sasaki, Keisuke; Hara, Satoshi; Yamakami, Reina; Sato, Yusuke; Hasegawa, Saki; Kono, Tomohiro; Morohaku, Kanako; Obata, Yayoi;
    Molecular Reproduction and Development, 86(6), 614-623, Jun. 2019WebofScience電子ジャーナル
  • Development of fertile mouse oocytes from mitotic germ cells in vitro
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    NATURE PROTOCOLS, 12(9), 1817-1829, Sep. 2017WebofScience電子ジャーナル
  • Development of fertile mouse oocytes from mitotic germ cells in vitro
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    NATURE PROTOCOLS, 12(9), 1817-1829, Sep. 2017WebofScience電子ジャーナル
  • Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    ANNALS OF BIOMEDICAL ENGINEERING, 45(7), 1608-1619, Jul. 2017WebofScience電子ジャーナル
  • Differentiation of Mouse Primordial Germ Cells into Functional Oocytes In Vitro
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    ANNALS OF BIOMEDICAL ENGINEERING, 45(7), 1608-1619, Jul. 2017WebofScience電子ジャーナル
  • A brief history of the search for the protein(s) involved in the acute regulation of steroidogenesis
    Stocco, Douglas M.; Zhao, Amy H.; Tu, Lan N.; Morohaku, Kanako; Selvaraj, Vimal;
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 441(C), 7-16, 2017WebofScience電子ジャーナル
  • A brief history of the search for the protein(s) involved in the acute regulation of steroidogenesis
    Stocco, Douglas M.; Zhao, Amy H.; Tu, Lan N.; Morohaku, Kanako; Selvaraj, Vimal;
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 441(C), 7-16, 2017WebofScience電子ジャーナル
  • Complete in vitro generation of fertile oocytes from mouse primordial germ cells
    Morohaku, Kanako; Tanimoto, Ren; Sasaki, Keisuke; Kawahara-Miki, Ryouka; Kono, Tomohiro; Hayashi, Katsuhiko; Hirao, Yuji; Obata, Yayoi;
    Proceedings of the National Academy of Sciences of the United States of America, 113(32), 9021-9026, Jul. 2016WebofScience電子ジャーナル
  • Developmental competence of oocytes grown in vitro: Has it peaked already?
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    Journal of Reproduction and Development, 62(1), 1-5, Feb. 2016WebofScience
  • Developmental competence of oocytes grown in vitro: Has it peaked already?
    Morohaku, Kanako; Hirao, Yuji; Obata, Yayoi;
    Journal of Reproduction and Development, 62(1), 1-5, Feb. 2016WebofScience
  • Mitochondrial Translocator Protein (TSPO) Function Is Not Essential for Heme Biosynthesis
    Zhao, Amy H.; Tu, Lan N.; Mukai, Chinatsu; Sirivelu, Madhu P.; Pillai, Viju V.; Morohaku, Kanako; Cohen, Roy; Selvaraj, Vimal;
    JOURNAL OF BIOLOGICAL CHEMISTRY, 291(4), 1591-1603, 2016WebofScience電子ジャーナル
  • Mitochondrial Translocator Protein (TSPO) Function Is Not Essential for Heme Biosynthesis
    Zhao, Amy H.; Tu, Lan N.; Mukai, Chinatsu; Sirivelu, Madhu P.; Pillai, Viju V.; Morohaku, Kanako; Cohen, Roy; Selvaraj, Vimal;
    JOURNAL OF BIOLOGICAL CHEMISTRY, 291(4), 1591-1603, 2016WebofScience電子ジャーナル
  • Complete in vitro generation of fertile oocytes from mouse primordial germ cells
    Morohaku, Kanako; Tanimoto, Ren; Sasaki, Keisuke; Kawahara-Miki, Ryouka; Kono, Tomohiro; Hayashi, Katsuhiko; Hirao, Yuji; Obata, Yayoi;
    Proceedings of the National Academy of Sciences of the United States of America, 113(32), 9021-9026, 2016WebofScience電子ジャーナル
  • Steroid Hormone Biosynthesis: TSPO Function Directly Challenged in Viable Null Mice
    Tu, Lan N.; Morohaku, Kanako; Manna, Pulak R.; Pelton, Susanne H.; Butler, W. Ronald; Stocco, Douglas M.; Selvaraj, Vimal;
    ENDOCRINE REVIEWS, 35(3), Jun. 2014WebofScience
  • Steroid Hormone Biosynthesis: TSPO Function Directly Challenged in Viable Null Mice
    Tu, Lan N.; Morohaku, Kanako; Manna, Pulak R.; Pelton, Susanne H.; Butler, W. Ronald; Stocco, Douglas M.; Selvaraj, Vimal;
    ENDOCRINE REVIEWS, 35(3), Jun. 2014WebofScience
  • Translocator Protein/Peripheral Benzodiazepine Receptor Is Not Required for Steroid Hormone Biosynthesis
    Morohaku, Kanako; Pelton, Susanne H.; Daugherty, Daniel J.; Butler, W. Ronald; Deng, Wenbin; Selvaraj, Vimal;
    Endocrinology, 155(1), 89-97, Jan. 2014WebofScience電子ジャーナル
  • Translocator Protein/Peripheral Benzodiazepine Receptor Is Not Required for Steroid Hormone Biosynthesis
    Morohaku, Kanako; Pelton, Susanne H.; Daugherty, Daniel J.; Butler, W. Ronald; Deng, Wenbin; Selvaraj, Vimal;
    Endocrinology, 155(1), 89-97, Jan. 2014WebofScience電子ジャーナル
  • Peripheral Benzodiazepine Receptor/Translocator Protein Global Knock-out Mice Are Viable with No Effects on Steroid Hormone Biosynthesis
    Tu, Lan N.; Morohaku, Kanako; Manna, Pulak R.; Pelton, Susanne H.; Butler, W. Ronald; Stocco, Douglas M.; Selvaraj, Vimal;
    JOURNAL OF BIOLOGICAL CHEMISTRY, 289(40), 27444-27454, 2014WebofScience電子ジャーナル
  • Peripheral Benzodiazepine Receptor/Translocator Protein Global Knock-out Mice Are Viable with No Effects on Steroid Hormone Biosynthesis
    Tu, Lan N.; Morohaku, Kanako; Manna, Pulak R.; Pelton, Susanne H.; Butler, W. Ronald; Stocco, Douglas M.; Selvaraj, Vimal;
    JOURNAL OF BIOLOGICAL CHEMISTRY, 289(40), 27444-27454, 2014WebofScience電子ジャーナル
  • Incorporation of Phosphatase Inhibitor in Culture Prompts Growth Initiation of Isolated Non-Growing Oocytes
    Morohaku, Kanako; Hoshino, Yumi; Sasada, Hiroshi; Sato, Eimei;
    PLOS ONE, 8(11), e77533, 2013WebofScience電子ジャーナル
  • Developmental Expression of Translocator Protein/Peripheral Benzodiazepine Receptor in Reproductive Tissues
    Morohaku, Kanako; Phuong, Newton S.; Selvaraj, Vimal;
    PLOS ONE, 8(9), e74509, 2013WebofScience電子ジャーナル
  • Incorporation of Phosphatase Inhibitor in Culture Prompts Growth Initiation of Isolated Non-Growing Oocytes
    Morohaku, Kanako; Hoshino, Yumi; Sasada, Hiroshi; Sato, Eimei;
    PLOS ONE, 8(11), 2013WebofScience電子ジャーナル
  • Developmental Expression of Translocator Protein/Peripheral Benzodiazepine Receptor in Reproductive Tissues
    Morohaku, Kanako; Phuong, Newton S.; Selvaraj, Vimal;
    PLOS ONE, 8(9), 2013WebofScience電子ジャーナル
  • Possible Candidates of Sperm Chemotatxis in Bulls, Boars and Goats
    Saito, Rie; Inada, Rino; Maruyama, Naho; Hamada, Asuka; Morohaku, Kanako; Momozawa, Kenji; Sasada, Hiroshi;
    BIOLOGY OF REPRODUCTION, 85, Jul. 2011WebofScience
  • Application of Chemically Defined Medium RD for In Vitro Maturation of Bovine Oocytes
    Momozawa, Kenji; Ooya, Kaori; Saito, Rie; Morohaku, Kanako; Sasada, Hiroshi;
    BIOLOGY OF REPRODUCTION, 85, Jul. 2011WebofScience
  • Addition of a Phosphatase Inhibitor Prompts Growth Initiation of Isolated Primordial Follicle Oocytes in Culture
    Morohaku, Kanako; Hoshino, Yumi; Sasada, Hiroshi; Sato, Eimei;
    BIOLOGY OF REPRODUCTION, 191-191, 2009WebofScience
  • Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV)
    Yamashiro, Hideaki; Narita, Kaori; Sugimura, Satoshi; Han, Young-Joon; Sugawara, Atsushi; Morohaku, Kanako; Nakazato, Fumiaki; Konno, Tsutomu; Yoshida, Muneyoshi; Sato, Eimei;
    Animal Reproduction Science, 102(1-2), 165-171, Nov. 2007WebofScience電子ジャーナル
  • Trehalose enhanced the freezability of Poodle dog sperm collected by an artificial vagina (AV)
    Yamashiro, Hideaki; Narita, Kaori; Sugimura, Satoshi; Han, Young-Joon; Sugawara, Atsushi; Morohaku, Kanako; Nakazato, Fumiaki; Konno, Tsutomu; Yoshida, Muneyoshi; Sato, Eimei;
    Animal Reproduction Science, 102(1-2), 165-171, Nov. 2007WebofScience電子ジャーナル

Lectures, oral presentations, etc.

  • Ex vivo engineering of egg production for birth of offspring
    Kanako Morohaku
    Japan-Poland Joint Seminar, 04 Sep. 2023, Japan Society for the Promotion of Science, Polish Academy of Science
  • 加齢マウス卵母細胞の体外発育培養: 若齢マウス顆粒層細胞との共培養
    小浜 智大, 富岡 郁夫, 諸白 家奈子
    第116回日本繁殖生物学会, Sep. 2023, 日本繁殖生物学会
  • Effects of Oxygen Concentrations on In Vitro Survival and Growth of Preantral Follicle-Derived Undeveloped Oocytes Co-Cultured with Granulosa Cells
    Tomohiro Kohama, Ikuo Tomioka, Kanako Morohaku
    Society for the Study of Reproduction 56th Annual Meeting, Jul. 2023, Society for the Study of Reproduction
  • Examination of culture conditions for reaggregation of mouse granulosa cells - oocyte complexes
    Tomohiro KOHAMA, Ikuo TOMIOKA, Kanako MOROHAKU
    115th SRD Meeting, Sep. 2022, the Society for Reproduction and Development
  • A new ART: egg production by ex vivo oocyte engineering
    Morohaku K.
    Cornell University, Reproductive Biology Seminar Series, Oct. 2021, Cornell University, Invited
  • The farnesoid X receptor (FXR) is involved in ovulatory response to superstimulation.
    Ikuo Tomioka, Yuka Tanahashi, Kanako Morohaku, Hiroshi Fujii
    Society for the Study of Reproduction, 52th Annual conference, Jul. 2019, Society for the Study of Reproduction
  • In vitro growth of early preantral follicles by two culture protocols
    Kanako Morohaku, Tomohiro Kohama
    Society for the Study of Reproduction, 52th Annual conference,, Jul. 2019, Society for the Study of Reproduction
  • Parental origin-derived primary memories are erased in Zfp57-deficient embryonic stem cells
    Keisuke Sasaki, Ai Nakajima, Kanako Morohaku, Yuta Asanuma, Yusuke Sotomaru, Tomohiro Kono, Yayoi Obata
    The 4th World Congress of Reproductive Biology (WCRB 2017), Sep. 2017
  • Temporal control of oocyte cyst breakdown and granulosa cell differentiation via inhibition of the estrogen pathway is required for normal follicle assembly
    Ren Tanimoto, Kanako Morohaku, Kono Tomohiro, Yuji Hirao, Yayoi Obata
    The 4th World Congress of Reproductive Biology (WCRB 2017), Sep. 2017
  • Abnormal Follicle Assembly In Vitro Correlates with Ectopic Expression of AMH in Mice
    Tanimoto, R., Morohaku, K., Sasaki, K., Kawahara-Miki, R., Kono, T., Hayashi, K., Hirao, Y., and Obata, Y.
    The Society for the Study of Reproduction 49th Annual Meeting, 2016
  • In vitro growth of primordial follicles derived from neonatal mouse ovaries
    Morohaku, K., Kono, T., Hirao, Y., and Obata, Y
    Gordon Research Conference, 2015
  • Steroid Hormone Biosynthesis: TSPO Function Directly Challenged in Viable Null Mice
    Tu, Lan N.; Morohaku, Kanako; Manna, Pulak R.; Pelton, Susanne H.; Butler, W. Ronald; Stocco, Douglas M.; Selvaraj, Vimal;
    ENDOCRINE REVIEWS, Jun. 2014
  • Steroid Hormone Biosynthesis: TSPO Function Directly Challenged in Viable Null Mice
    Tu, L.N., Morohaku, K., Manna, P., Pelton, S.H., Butler, W.R., Stocco, D.M. and Selvaraj, V
    ICE/ENDO, 2014
  • Translocator protein/ Peripheral benzodiazepine receptor deficiency does not compromise steroid hormone biosynthesis or erythropoiesis
    Tu, L.N., Morohaku, K., Sirivelu, M.P., Pelton, S.H., Butler, W.R., Selvaraj, V.
    Annual meeting of ASCB, 2013
  • Mitochondrial Translocator Protein Is Not Essential But Can Facilitate The Biosynthesis Of Protoporphyrin IX
    Zhao, A. H., Tu, L. N. L., Mukai, C., Morohaku, K., and Selvaraj, V.
    Annual meeting of ASCB, 2013
  • Mitochondrial Translocator Protein/Peripheral Benzodiazepine Receptor Is Not Required For Steroid Hormone Biosynthesis
    Morohaku, K., Pelton, S.H., Daugherty, D.J., Butler, W. R., Deng, W., Selvaraj, V.
    Annual meeting of ASCB, 2013
  • Developmental Expression of Translocator Protein (TSPO) in the Murine Gonads
    Morohaku, K. and Selvaraj, V.
    The Society for the Study of Reproduction 45th Annual Meeting, 2012
  • Possible Candidates of Sperm Chemotatxis in Bulls, Boars and Goats
    Saito, Rie; Inada, Rino; Maruyama, Naho; Hamada, Asuka; Morohaku, Kanako; Momozawa, Kenji; Sasada, Hiroshi;
    BIOLOGY OF REPRODUCTION 85 -, Jul. 2011
  • Application of Chemically Defined Medium RD for In Vitro Maturation of Bovine Oocytes
    Momozawa, Kenji; Ooya, Kaori; Saito, Rie; Morohaku, Kanako; Sasada, Hiroshi;
    BIOLOGY OF REPRODUCTION 85 -, Jul. 2011
  • Addition of a Phosphatase Inhibitor Prompts Growth Initiation of Isolated Primordial Follicle Oocytes in Culture
    Morohaku, Kanako; Hoshino, Yumi; Sasada, Hiroshi; Sato, Eimei;
    BIOLOGY OF REPRODUCTION 191-191, 2009