論文 Protein Engineering of the Soluble Metal-dependent Formate Dehydrogenase from Escherichia coli Analytical Sciences,37(5):733-739 2021 Author:Rintaro FUJI, Koji UMEZAWA, Manami MIZUGUCHI, Masaki IHARA
Production of extracellular polysaccharides and phycobiliproteins from Tolypothrix sp. PCC7601 using mechanical milking systems 2020 Author:Alice Uchida, Yukiko Higashi, Shota Yamamoto, Jun Nakanishi, Naoki Kanayama, Kazuhiro Shibata, Masaki Ihara
A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells 2019 Author:Mitsuyoshi Miyata, Shunsuke Iwata, Chie Mifude, Kazuyuki Matsushima, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda
Overexpression of endogenous 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation Journal of Bioscience and Bioengineering,123(5):590-596 2017 Author:Kai Kudoh, Shingo Hotta, Midori Sekine, Arisu Uchida, Genma Kubota, Rintaro Fujii, Yusuke Kawano, Masaki Ihara
Exploration of the 1-deoxy-D-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system Journal of Bioscience and Bioengineering,123(3):300-307 2017 Author:Kudoh Kai, Kubota Genma, Fujii Rintaro, Kawano Yusuke, Ihara Masaki*
Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis Applied Microbiology and Biotechnology,101(1):341-349 2017 Author:Shigemori S, Ihara M, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T
Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-D-xylulose 5-phosphate synthase and carbon allocation analysis Journal of Bioscience and Bioengineering,118:20-28 2014
Identification and characterization of UDP-glucose pyrophosphorylase in Anabaena sp. PCC 7120 Journal of Bioscience and Bioengineering,117:531-538 2014
Expression of an antibody-enzyme complex by the L-chain fusion method Journal of Bioscience and Bioengineering,116:17-21 2013 Author:Chungyong Han, Masaki Ihara, and Hiroshi
A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases Analytical Chemistry,85:7985-7940 2013
Light Driven CO2 Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase PLOS ONE,8(8):e71581 2013 Author:Ihara, M; Kawano, Y; Urano, M; Okabe, A
Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments Bioconjugate Chemistry,23:1463-1469 2012 Author:Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, and Hiroshi Ueda
“Quenchbodies”: quench-based antibody probes that show antigen-dependent fluorescence J. Am. Chem. Soc.,133:17386 2011 Author:R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka and H. Ueda
Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules Bioconjugate chemistry,:633-641 2011 Author:Kojima et al.
Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4 Analytical Chemistry,83:1008-1014 2011 Author:Islam et al.
Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells Journal of Immunological Methods,361:57-63 2010 Author:SasaJima et al.
Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples Bioanalysis,2:1683-1687 2010 Author:Islam et al.
Antibody affinity maturation in vitro using unconjugated peptide antigen Protein Engineering Design and Selection,23:185-193 2010 Author:Iwai et al.
Antibody affinity maturation in vitro using unconjugated peptide antigen Lab on a Chip,10:92-100 2010 Author:Ihara et al.
Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol Analytical Chemistry,81:8298-8304 2009 Author:Ihara et al.
Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen Analytical Chemistry,81:7532-7537 2009 Author:Sakata et al.
Trans-splicing as a novel method to rapidly produce antibody fusion proteins Biochemical and Biophysical Research Communications,384:316-321 2009 Author:Iwasaki et al.
Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology, Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils Biophysical Journal,96:3331-3340 2009 Author:Zako et al.
Antibody Fab display system that can perform open sandwich ELISA Analytical Biochemistry,386:36-44 2009 Author:Dong et al.
Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase Photochemical andPhotobiology,82:1677-1685 2006 Author:Ihara et al.
Design of Artificial Hydrogenase-Photosystem I Complex for Photo-driven Hydrogen Production by Cyanobacteria or Microalga Photochemical and Photobiology,82:676-682 2006 Author:Ihara et al.
Functions of fluctuation in the heme-binding loops of cytochrome b5 revealed in the process of heme incorporation 2000 Author:Masaki Ihara, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima
Conversion of an electron-transfer protein into an oxygen binding protein: The axial cytochrome b5 mutant with an unusually high O2 affinity 2000 Author:Masaki Ihara, Masato Shintaku, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima