IHARA Masaki

Academic Assembly School of Science and Technology Institute of Agriculture

Interdisciplinary Cluster for Cutting Edge Research Institute for Biomedical Sciences 

Associated Professor 

Degree

  • 工学博士

Research Keyword

    Protein, Biofuel, Microorganism, Photosystem

Field Of Study

  • Protein Engineering
  • Metabolic Engineering
  • Algae
  • Carbon fixation

Mail Address

    m_ihara★shinshu-u.ac.jp

Career

  • 2002 - 2004
    日本学術振興機構特別研究員

Educational Background

  • 1996 - 2002, 京都大学
  • 1992 - 1996, 岡山大学

Award

  • 2006
    GSCポスター賞
  • 2006
    GRC Green Chemistry selected poster presenter

Paper

  • Protein Engineering of the Soluble Metal-dependent Formate Dehydrogenase from Escherichia coli
    Rintaro FUJI, Koji UMEZAWA, Manami MIZUGUCHI, Masaki IHARA
    Analytical Sciences, 37(5), 733-739, 2021
  • Production of extracellular polysaccharides and phycobiliproteins from Tolypothrix sp. PCC7601 using mechanical milking systems
    Alice Uchida, Yukiko Higashi, Shota Yamamoto, Jun Nakanishi, Naoki Kanayama, Kazuhiro Shibata, Masaki Ihara
    2020
  • A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells
    Mitsuyoshi Miyata, Shunsuke Iwata, Chie Mifude, Kazuyuki Matsushima, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda
    2019
  • Exploration of the 1-deoxy-D-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system
    Kudoh Kai, Kubota Genma, Fujii Rintaro, Kawano Yusuke, Ihara Masaki*
    Journal of Bioscience and Bioengineering, 123(3), 300-307, 2017
  • Overexpression of endogenous 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation
    Kai Kudoh, Shingo Hotta, Midori Sekine, Arisu Uchida, Genma Kubota, Rintaro Fujii, Yusuke Kawano, Masaki Ihara
    Journal of Bioscience and Bioengineering, 123(5), 590-596, 2017
  • Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
    Shigemori S, Ihara M, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(1), 341-349, 2017WebofScienceリポジトリ電子ジャーナル
  • 生物触媒を利用した半人工光合成
    伊原正喜
    化学と工業, 69(11), 963-965, 2016
  • Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases
    Mol. Biotechnol, 56, 953-961, 2015
  • Enhancement of L-cysteine production by disruption of yciW in Escherichia coli
    Journal of Bioscience and Bioengineering, 119, 176-179, 2015
  • Expression Systems for Soluble Metal-Dependent Formate Dehydrogenase
    Journal of Photochemistry & Photobiology, A: Chemistry, 2015
  • Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice
    Suguru Shigemori, Takafumi Watanabe, Kai Kudoh, Masaki Ihara, Shireen Nigar, Yoshinari Yamamoto, Yoshihito Suda, Takashi Sato, Haruki Kitazawa* and Takeshi Shimosato*
    Microbial Cell Factories, 14, 189, 2015
  • Identification and characterization of UDP-glucose pyrophosphorylase in Anabaena sp. PCC 7120
    Journal of Bioscience and Bioengineering, 117, 531-538, 2014
  • Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-D-xylulose 5-phosphate synthase and carbon allocation analysis
    Journal of Bioscience and Bioengineering, 118, 20-28, 2014
  • 微細藻類によるバイオエネルギー及びバイオマテリアル生産
    伊原正喜
    月刊技術士, 595(9), 12-15, 2014
  • A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases
    Analytical Chemistry, 85, 7985-7940, 2013
  • Expression of an antibody-enzyme complex by the L-chain fusion method
    Chungyong Han, Masaki Ihara, and Hiroshi
    Journal of Bioscience and Bioengineering, 116, 17-21, 2013
  • Light Driven CO2 Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
    Ihara, M; Kawano, Y; Urano, M; Okabe, A
    PLOS ONE, 8(8), e71581, 2013WebofScienceリポジトリ電子ジャーナル
  • Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments
    Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, and Hiroshi Ueda
    Bioconjugate chemistry, 23, 1463-1469, 2012
  • Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4
    Islam et al.
    Analytical Chemistry, 83, 1008-1014, 2011
  • Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules
    Kojima et al.
    Bioconjugate chemistry, 633-641, 2011
  • “Quenchbodies”: quench-based antibody probes that show antigen-dependent fluorescence
    R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka and H. Ueda
    J. Am. Chem. Soc., 133, 17386, 2011
  • Antibody affinity maturation in vitro using unconjugated peptide antigen
    Ihara et al.
    Lab on a Chip, 10, 92-100, 2010
  • Antibody affinity maturation in vitro using unconjugated peptide antigen
    Iwai et al.
    Protein Engineering Design and Selection, 23, 185-193, 2010
  • Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
    Islam et al.
    Bioanalysis, 2, 1683-1687, 2010
  • Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells
    SasaJima et al.
    JOURNAL OF IMMUNOLOGICAL METHODS, 361, 57-63, 2010
  • Antibody Fab display system that can perform open sandwich ELISA
    Dong et al.
    ANALYTICAL BIOCHEMISTRY, 386, 36-44, 2009
  • Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology, Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils
    Zako et al.
    BIOPHYSICAL JOURNAL, 96, 3331-3340, 2009
  • Trans-splicing as a novel method to rapidly produce antibody fusion proteins
    Iwasaki et al.
    Biochemical and biophysical research communications, 384, 316-321, 2009
  • Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen
    Sakata et al.
    Analytical Chemistry, 81, 7532-7537, 2009
  • Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol
    Ihara et al.
    Analytical Chemistry, 81, 8298-8304, 2009
  • Design of Artificial Hydrogenase-Photosystem I Complex for Photo-driven Hydrogen Production by Cyanobacteria or Microalga
    Ihara et al.
    Photochemical and Photobiology, 82, 676-682, 2006
  • Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase
    Ihara et al.
    Photochemical andPhotobiology, 82, 1677-1685, 2006
  • Conversion of an electron-transfer protein into an oxygen binding protein: The axial cytochrome b5 mutant with an unusually high O2 affinity
    Masaki Ihara, Masato Shintaku, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima
    2000
  • Functions of fluctuation in the heme-binding loops of cytochrome b5 revealed in the process of heme incorporation
    Masaki Ihara, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima
    2000

MISC

  • 天然の光合成を凌駕した光水素生産バイオナノデバイス
    伊原正喜
    月刊化学, (6月号), 2012

Books and other publications

  • 二酸化炭素の分離・回収・貯蔵技術の開発
    伊原正喜, 微生物によるカーボンリサイクル
    情報機構, 未定 2022
  • アグリバイオ10月号
    ギ酸資化菌を利用したカーボンリサイクル
    北隆館, 42-44 2022
  • アグリバイオ10月号
    山にメガソーラーを建設するのはタブーか
    北隆館, 45-49 2022
  • 人工光合成 実用化に向けた最新技術
    伊原正喜・河野祐介, 光合成の改造と、エネルギー生産系への挑戦
    情報機構, 270-278 2013
  • バイオサイエンスシリーズ「光合成のエネルギー・物質変換 人工光合成を目指して」
    伊原正喜, 人工光合成とエネルギー変換材料への転換
    2012
  • 化学工業 63
    伊原正喜・河野祐介, 天然光合成の改造と、エネルギー物質生産藻類の創製
    化学工業社), 31-38 2012
  • 信州大学農学部紀要
    伊原正喜, ヒドロゲナーゼの安定化・酸素耐性化
    2011
  • Kagaku Keizai 58
    M. Ihara, Develpment of energy producing phototropha
    93 2011
  • 化学と教育誌 56
    伊原正喜, 水素を作るたんぱく質
    32-35 2008
  • 化学工学 72
    伊原正喜・上田宏, 第三の免疫測定法「オープンサンドイッチ法」の開発
    486-488 2008
  • ケミカルバイオエンジニアリング 52
    伊原正喜, 光合成微生物の水素発生能の効率化
    249-257 2007

Courses

  • 有機分析化学演習
    Shinshu University
  • 外書講読
    Shinshu University
  • 有機分析化学
    Shinshu University

Affiliated academic society

  • 日本蛋白質科学会
  • 日本生物工学会
  • 日本農芸化学会

Research Themes

  • 共同研究, イビデン
    2022
  • 共同研究, エイコーエプソン
    2021
  • 共同研究, KDDI
    2021
  • 共同研究, 熊谷組
    2020
  • ギ酸代謝関連遺伝子の探索とそれらを利用した高速ギ酸資化菌の創出
    共同研究, 科研費基盤C
    2019 - 2022
  • ギ酸デヒドロゲナーゼを用いた新規人工炭酸固定系
    共同研究, 科研費基盤C
    2017 - 2019
  • ヒドロゲナーゼの改良を目指した分子進化工学的手法の開発
    共同研究, 科研費若手B
    2013 - 2016
  • 蛋白質工学によるギ酸生産藻類の創製
    科学研究費補助金, JST
    Oct. 2009 - Mar. 2015
  • タンパク質工学によるギ酸生産藻類の創出
    共同研究, JSTさきがけ
    2009 - 2015
  • 共同研究, 富士フイルム
    2006 - 2009

Industrial Property Rights

  • 抗体を選別するためのベクター, 5610378, Patent right
    川上雅之・上田宏・董金華・伊原正喜
  • 抗体と蛋白質との融合蛋白質の製造方法, 5487570, Patent right
    森寿弘・上田宏・韓忠勇・伊原正喜
  • 抗チロキシン抗体及びそれを用いた免疫測定方法, 5641464, Patent right
    上田宏・伊原正喜・カムルーン ナハル イスラム・森寿弘・笠置典之
  • 断片化抗体固定化担体及びその製造方法, 5518450, Patent right
    南高一・都築博彦・上田宏・伊原正喜
  • 分子間の相互作用の検出方法, 5754795, Patent right
    上田宏・伊原正喜・松山有紀・森寿弘
  • 抗原検出方法及び抗原検出装置, 5646219, Patent right
    南高一・都築博彦・上田宏・伊原正喜
  • 目的物質の測定方法, 4956715, Patent right
    川上雅之・上田宏・伊原正喜・小島 美樹・ペーテル・クリステンセン
  • 蛍光免疫測定方法, PCT/JP2010/006809, Patent right
    上田宏・阿部亮二・伊原正喜・高木広明
  • 分子構造の再現方法, 6571319, Patent right
    伊原正喜
  • 断片化抗体固定化担体及びその製造方法, 5371690, Patent right
    南高一・都築博彦・上田宏・伊原正喜
  • 酸化還元酵素と光合成反応中心との連結体による光駆動物質生産法, 4614053, Patent right
    伊原正喜・西原宏史

Social Contribution Activities

  • サイエンスカフェ
    いなっせ(伊那市), Oct. 2010 - Oct. 2010

Thesis guidance

  • 2010, 3, 3