IHARA Masaki
Academic Assembly School of Science and Technology Institute of Agriculture
Interdisciplinary Cluster for Cutting Edge Research Institute for Biomedical Sciences
Associated Professor
Researcher Information
Research Keyword
- Protein, Microorganism, Photosystem, Biofuel
Mail Address
- m_ihara★shinshu-u.ac.jp
Research activity information
Paper
- Protein Engineering of the Soluble Metal-dependent Formate Dehydrogenase from Escherichia coli
Rintaro FUJI, Koji UMEZAWA, Manami MIZUGUCHI, Masaki IHARA
Analytical Sciences, 37(5), 733-739, 2021 - Production of extracellular polysaccharides and phycobiliproteins from Tolypothrix sp. PCC7601 using mechanical milking systems
Alice Uchida, Yukiko Higashi, Shota Yamamoto, Jun Nakanishi, Naoki Kanayama, Kazuhiro Shibata, Masaki Ihara
2020 - A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells
Mitsuyoshi Miyata, Shunsuke Iwata, Chie Mifude, Kazuyuki Matsushima, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda
2019 - Exploration of the 1-deoxy-D-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system
Kudoh Kai, Kubota Genma, Fujii Rintaro, Kawano Yusuke, Ihara Masaki*
Journal of Bioscience and Bioengineering, 123(3), 300-307, 2017 - Overexpression of endogenous 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation
Kai Kudoh, Shingo Hotta, Midori Sekine, Arisu Uchida, Genma Kubota, Rintaro Fujii, Yusuke Kawano, Masaki Ihara
Journal of Bioscience and Bioengineering, 123(5), 590-596, 2017 - Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
Shigemori S, Ihara M, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(1), 341-349, 2017
- 生物触媒を利用した半人工光合成
伊原正喜
化学と工業, 69(11), 963-965, 2016 - Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases
Mol. Biotechnol, 56, 953-961, 2015 - Enhancement of L-cysteine production by disruption of yciW in Escherichia coli
Journal of Bioscience and Bioengineering, 119, 176-179, 2015 - Expression Systems for Soluble Metal-Dependent Formate Dehydrogenase
Journal of Photochemistry & Photobiology, A: Chemistry, 2015 - Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice
Suguru Shigemori, Takafumi Watanabe, Kai Kudoh, Masaki Ihara, Shireen Nigar, Yoshinari Yamamoto, Yoshihito Suda, Takashi Sato, Haruki Kitazawa* and Takeshi Shimosato*
Microbial Cell Factories, 14, 189, 2015 - Identification and characterization of UDP-glucose pyrophosphorylase in Anabaena sp. PCC 7120
Journal of Bioscience and Bioengineering, 117, 531-538, 2014 - Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-D-xylulose 5-phosphate synthase and carbon allocation analysis
Journal of Bioscience and Bioengineering, 118, 20-28, 2014 - 微細藻類によるバイオエネルギー及びバイオマテリアル生産
伊原正喜
月刊技術士, 595(9), 12-15, 2014 - A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases
Analytical Chemistry, 85, 7985-7940, 2013 - Expression of an antibody-enzyme complex by the L-chain fusion method
Chungyong Han, Masaki Ihara, and Hiroshi
Journal of Bioscience and Bioengineering, 116, 17-21, 2013 - Light Driven CO2 Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
Ihara, M; Kawano, Y; Urano, M; Okabe, A
PLOS ONE, 8(8), e71581, 2013 - Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments
Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, and Hiroshi Ueda
Bioconjugate chemistry, 23, 1463-1469, 2012 - Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4
Islam et al.
Analytical Chemistry, 83, 1008-1014, 2011 - Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules
Kojima et al.
Bioconjugate chemistry, 633-641, 2011 - “Quenchbodies”: quench-based antibody probes that show antigen-dependent fluorescence
R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka and H. Ueda
J. Am. Chem. Soc., 133, 17386, 2011 - Antibody affinity maturation in vitro using unconjugated peptide antigen
Ihara et al.
Lab on a Chip, 10, 92-100, 2010 - Antibody affinity maturation in vitro using unconjugated peptide antigen
Iwai et al.
Protein Engineering Design and Selection, 23, 185-193, 2010 - Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
Islam et al.
Bioanalysis, 2, 1683-1687, 2010 - Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells
SasaJima et al.
JOURNAL OF IMMUNOLOGICAL METHODS, 361, 57-63, 2010 - Antibody Fab display system that can perform open sandwich ELISA
Dong et al.
ANALYTICAL BIOCHEMISTRY, 386, 36-44, 2009 - Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology, Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils
Zako et al.
BIOPHYSICAL JOURNAL, 96, 3331-3340, 2009 - Trans-splicing as a novel method to rapidly produce antibody fusion proteins
Iwasaki et al.
Biochemical and biophysical research communications, 384, 316-321, 2009 - Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen
Sakata et al.
Analytical Chemistry, 81, 7532-7537, 2009 - Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol
Ihara et al.
Analytical Chemistry, 81, 8298-8304, 2009 - Design of Artificial Hydrogenase-Photosystem I Complex for Photo-driven Hydrogen Production by Cyanobacteria or Microalga
Ihara et al.
Photochemical and Photobiology, 82, 676-682, 2006 - Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase
Ihara et al.
Photochemical andPhotobiology, 82, 1677-1685, 2006 - Conversion of an electron-transfer protein into an oxygen binding protein: The axial cytochrome b5 mutant with an unusually high O2 affinity
Masaki Ihara, Masato Shintaku, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima
2000 - Functions of fluctuation in the heme-binding loops of cytochrome b5 revealed in the process of heme incorporation
Masaki Ihara, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima
2000
Books and other publications
- 二酸化炭素の分離・回収・貯蔵技術の開発
伊原正喜, 微生物によるカーボンリサイクル
情報機構, 未定 2022アグリバイオ10月号
ギ酸資化菌を利用したカーボンリサイクル
北隆館, 42-44 2022アグリバイオ10月号
山にメガソーラーを建設するのはタブーか
北隆館, 45-49 2022人工光合成 実用化に向けた最新技術
伊原正喜・河野祐介, 光合成の改造と、エネルギー生産系への挑戦
情報機構, 270-278 2013バイオサイエンスシリーズ「光合成のエネルギー・物質変換 人工光合成を目指して」
伊原正喜, 人工光合成とエネルギー変換材料への転換
2012化学工業 63
伊原正喜・河野祐介, 天然光合成の改造と、エネルギー物質生産藻類の創製
化学工業社）, 31-38 2012信州大学農学部紀要
伊原正喜, ヒドロゲナーゼの安定化・酸素耐性化
2011Kagaku Keizai 58
M. Ihara, Develpment of energy producing phototropha
93 2011化学と教育誌 56
伊原正喜, 水素を作るたんぱく質
32-35 2008化学工学 72
伊原正喜・上田宏, 第三の免疫測定法「オープンサンドイッチ法」の開発
486-488 2008ケミカルバイオエンジニアリング 52
伊原正喜, 光合成微生物の水素発生能の効率化
249-257 2007Research Themes
- 共同研究, イビデン
2022 - 共同研究, エイコーエプソン
2021 - 共同研究, KDDI
2021 - 共同研究, 熊谷組
2020 - ギ酸代謝関連遺伝子の探索とそれらを利用した高速ギ酸資化菌の創出
共同研究, 科研費基盤C
2019 - 2022 - ギ酸デヒドロゲナーゼを用いた新規人工炭酸固定系
共同研究, 科研費基盤C
2017 - 2019 - ヒドロゲナーゼの改良を目指した分子進化工学的手法の開発
共同研究, 科研費若手B
2013 - 2016 - 蛋白質工学によるギ酸生産藻類の創製
科学研究費補助金, JST
Oct. 2009 - Mar. 2015 - タンパク質工学によるギ酸生産藻類の創出
共同研究, JSTさきがけ
2009 - 2015 - 共同研究, 富士フイルム
2006 - 2009
Industrial Property Rights
- 抗体を選別するためのベクター, 5610378, Patent right
川上雅之・上田宏・董金華・伊原正喜 - 抗体と蛋白質との融合蛋白質の製造方法, 5487570, Patent right
森寿弘・上田宏・韓忠勇・伊原正喜 - 抗チロキシン抗体及びそれを用いた免疫測定方法, 5641464, Patent right
上田宏・伊原正喜・カムルーン ナハル イスラム・森寿弘・笠置典之 - 断片化抗体固定化担体及びその製造方法, 5518450, Patent right
南高一・都築博彦・上田宏・伊原正喜 - 分子間の相互作用の検出方法, 5754795, Patent right
上田宏・伊原正喜・松山有紀・森寿弘 - 抗原検出方法及び抗原検出装置, 5646219, Patent right
南高一・都築博彦・上田宏・伊原正喜 - 目的物質の測定方法, 4956715, Patent right
川上雅之・上田宏・伊原正喜・小島 美樹・ペーテル・クリステンセン - 蛍光免疫測定方法, PCT/JP2010/006809, Patent right
上田宏・阿部亮二・伊原正喜・高木広明 - 分子構造の再現方法, 6571319, Patent right
伊原正喜 - 断片化抗体固定化担体及びその製造方法, 5371690, Patent right
南高一・都築博彦・上田宏・伊原正喜 - 酸化還元酵素と光合成反応中心との連結体による光駆動物質生産法, 4614053, Patent right
伊原正喜・西原宏史
Educational activity information