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桜井 敬之  サクライ タカユキ

教員組織学術研究院(医学系)電話番号0263-37-2578
教育組織先鋭領域融合研究群 バイオメディカル研究所 ライフイノベーション部門FAX番号0263-37-2564
職名准教授メールアドレスtsakurai*shinshu-u.ac.jp
住所〒390-8621 長野県松本市旭3-1-1ホームページURLhttp://www7a.biglobe.ne.jp/~shindo/

プロフィール

研究分野
発生生物学
発生工学
心血管生物学
ゲノム編集
キーワード:発生工学 , ゲノム編集 , 発生生物学 , 遺伝子改変動物
現在の研究課題
心血管生物学におけるアドレノメデュリン-RAMPシステムの機能解明
キーワード:アドレノメデュリン , Ramp , 心血管 , 炎症
新規疾患モデルマウスの開発

新規遺伝子操作技術の開発

新規胚操作技術の開発

所属学会
所属学会
日本分子生物学会
日本再生医療学会
日本ゲノム編集学会
学歴
出身大学院
1995 , 横浜市立大学大学院総合理学研究科
1992 , 静岡大学大学院理学研究科

出身学校・専攻等(大学院を除く)
1990 , 静岡大学理学部生物学科

取得学位
理学博士 , 横浜市立大学
理学修士 , 静岡大学
研究職歴等
研究職歴
2019- , 先鋭領域融合研究群バイオメディカル研究所ライフイノベーション部門 准教授
2008- , 信州大学大学院医学系研究科准教授
2005-2008 , 信州大学大学院医学系研究科講師
1999-2005 , 東海大学医学部助手

研究職歴以外の職歴
2000-2004 , 東海大学遺伝子工学実験動物研究センター常駐管理者(兼)

研究活動業績

研究業績(著書・
発表論文等)
書籍等出版物
Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line, Microinjection: Methods and Protocols Methods in Molecular Biology , Chapter 15
Springer Nature 2019
Author:Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B Gurumurthy, Masahiro Sato, Takayuki Shindo


Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals, Results and Problems in Cell Differentiation 63 , Chapter 21
Springer 2017
Author:Takayuki Sakurai, Takayuki Shindo, Masahiro Sato


アドレノメデュリン-RAMP2シグナル, 細胞工学 35 1, 33-38
2016
Author:新藤隆行、桜井敬之、神吉昭子、市川優佳、田中愛、小山晃英


アドレノメデュリンによる血管形成の調節-遺伝子操作動物における検討ー, 医学のあゆみ 223 13, 1063-1068
医歯薬出版 2007
Author:新藤隆行 桜井敬之


マウスの卵母細胞と着床前胚に対するRNAi実験入門, RNAi A GUIDE TO GENE SILENCING, 303-333
メディカルサイエンスインターナショナル 2004
Author:桜井敬之 木村穣 (訳)


論文
エネルギー代謝制御におけるAM, AM2の機能分化と相互作用
Medical Science Digest,48(6):306-308 2022
Author:神吉昭子、桜井敬之、新藤隆行


Receptor activity modifying protein RAMP sub-isoforms and their functional differentiation, which regulates functional diversity of adrenomedullin
Biology,11(5):788 2022
Author:Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Sakurai T


An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility
Sci Rep,12(1):8844 2022
Author:Negishi K, Aizawa K, Shindo T, Suzuki T, Sakurai T, Saito Y, Miyakawa Y, Tanokura M, Kataoka Y, Maeda M, Shota Tomida, Hiroyuki Morita, Takeda N, Komuro I, Kario K, Nagai R, Imai Y


Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing
STAR Protoc,2(2) 2021
Author:Sakurai T, Shindo T


Adrenomedullin Ameliorates Pulmonary Fibrosis by Regulating TGF-ß-Smads Signaling and Myofibroblast Differentiation
Endocrinology,162(8):1-22 2021
Author:Wei Y, Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Cui N, Kakihara S, Zhao, Y, Aruga K, Sanjo H, Shindo T


Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress
Endocrinology,162(3):1-20 2021
Author:Cui N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Tanaka M, Wei Y, Kakihara S, Zhao Y, Aruga K, Kawagishi H, Nakada T, Yamada M, Shindo T


Adrenomedullin-RAMP2 system ameliorates subretinal fibrosis by suppressing epithelial-mesenchymal transition in age-related macular degeneration
Am J Pathol,191(4):652-668 2021
Author:Tanaka M, Kakihara S, Hirabayashi K, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Cui N, Wei Y, Zhao Y, Aruga K, Yamauchi A, Murata T, Shindo T


アドレノメデュリン-RAMP3系欠損は、癌関連線維芽細胞のポドプラニン(PDPN)発現を抑制し、臓器間転移を抑制する
血管,43(3):1-8 2020
Author:戴昆, 田中愛, 神吉昭子, 桜井敬之, 市川優佳, 河手久香, 崔南奇, 田中正明, Wei Yangxuan, 柿原伸次, 新藤隆行


Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts.
Oncogene,39(10):1914-1930 2020
Author:Dai, K.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Matsui, S.; Shindo, T.


Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
Sci.Rep,10(1):1091 2020
Author:Sakurai, T.; Kamiyoshi, A.; Kawate, H.; Watanabe, S.; Sato, M.; Shindo, T.


Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice.
Heliyon,5:e02655 2019
Author:Yamauchi, A.; Kamiyoshi, A.; Sakurai, T.; Miyazaki, H.; Hirano, E.; Lim, HS.; Kaku, T.; Shindo, T.


Endogenous calcitonin gene-related peptide deficiency exacerbates postoperative lymphedema by suppressing lymphatic capillary formation and M2 macrophage accumulation.
Am J Pathol.,,189:2487-2502 2019
Author:Matsui, S.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Dai, K.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Nakamura, K.; Yamauchi, A.; Ishida, K.; Tanaka, S.; Kawamata, M.; Shindo, T.


Development of a novel model of central retinal vascular occlusion and the therapeutic potential of the adrenomedullin-receptor activity-modifying protein 2 system.
Am J Pathol.,,189:449-466 2019
Author:Hirabayashi, K.; Tanaka, M.; Imai, A.; Toriyama, Y.; Iesato, Y.; Sakurai, T.; Kamiyoshi, A.; Ichikawa-Shindo, Y.; Kawate, H.; Tanaka, M.; Dai, K.; Cui, N.; Wei, Y.; Nakamura, K.; Iida, S.; Matsui, S.; Yamauchi, A.; Murata, T.; Shindo, T.


Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid-gestational murine fetuses.
IUBMB Life,71(7):835-844 2019
Author:Nakamura S, Ishihara M, Ando N, Watanabe S, Sakurai T, Sato M


Regulation of cardiovascular development and homeostasis by the adrenomedullin-RAMP system
Peptides,111:55-61 2019
Author:Shindo T, Tanaka M, Kamiyoshi, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Sakurai T


Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
Sci Rep,9(1):9923 2019
Author:Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.


Endogenous calcitonin gene-related peptide suppresses ischemic brain injuries and progression of cognitive decline.
J Hypertens,36(4):876-891 2018
Author:Zhai L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Xian X, Hirabayashi K, Dai K, Cui N, Tanimura K, Liu T, Wei Y, Tanaka M, Tomiyama H, Yamauchi A, Igarashi K, Shindo T.


Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Theriogenology,108:29-38 2018
Author:Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.


The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.
Int J Mol Sci.,19(4):E1075 2018
Author:Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M.


In vivo genome editing targeted towards the female reproductive system.
Arch Pharm Res.,41(9):898-910 2018
Author:Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Gurumurthy CB


RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders
Peptides,110:10-18 2018
Author:Liu T, Kamiyoshi A, Tanaka M, Iida S, Sakurai T, Ichikawa-Shindo Y, Kawate H, Hirabayashi K, Dai K, Cui N, Tanaka M, Wei Y, Nakamura K, Matsui S, Yamauchi A, Shindo T


Endogenous Calcitonin Gene-Related Peptide Regulates Lipid Metabolism and Energy Homeostasis in Male Mice.
Endocrinology.,158(5):1194-1206 2017
Author:Liu T, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Yang L, Tanaka M, Xian X, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.


Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Endocrinology.,158(5):1359-1372 2017
Author:Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.


Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy.
Am J Pathol.,187(5):999-1015 2017
Author:Imai A, Toriyama Y, Iesato Y, Hirabayashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Liu T, Xian X, Zhai L, Dai K, Tanimura K, Liu T, Cui N, Yamauchi A, Murata T, Shindo T.


Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Int J Mol Sci.,18(12):E2610 2017
Author:Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.


A non-inheritable maternal Cas9-based multiple-gene editing system in mice
Sci.Rep,6:srep20011 2016
Author:Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T


Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques
Sci.Rep,6:srep35861 2016
Author:Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K


Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury
Hypertension,67:99-106 2016
Author:Ayuzawa N, Nagase M, Ueda K, Nishimoto M, Kawarazaki W, Marumo T, Aiba A, Sakurai T, Shindo T, Fujita T


The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
,17:E1424 2016
Author:Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K


The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis
Cardiovasc.Res,111:398-409 2016
Author:Tanaka M, Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Liu T, Xian X, Imai A, Zhai L, Hirabayashi K, Owa S, Yamauchi A, Igarashi K, Taniguchi S, Shindo T


Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
Int.J.Mol.Sci,16:17838-17856 2016
Author:Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K


Generation of α-1,3-Galactosyltransferase-Deficient Porcine Embryonic Fibroblasts by CRISPR/Cas9-Mediated Knock-in of a Small Mutated Sequence and a Targeted Toxin-Based Selection System
Reprod Domest Anim,50:872-880 2015
Author:Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S.


A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
Biotechnol J,10:143-153 2015
Author:Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S


Adrenomedullin-RAMP2 System in Vascular Endothelial Cells
J Atheroscler Thromb,22:647-653 2015
Author:Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Shindo T


Pathophysiological function of endogenous calcitonin gene-related peptide in ocular vascular diseases
Am J Pathol,185:1783-1794 2015
Author:Toriyama Y, Iesato Y, Imai A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Igarashi K, Tanaka M, Liu T, Xian X, Zhai L, Owa S, Murata T, Shindo T


Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Biotechnol J,8:1355-1361 2014
Author:Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S


The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Xenotransplantation,21:291-300 2014
Author:Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S


アドレノメデュリン-RAMP2システムによる,白色脂肪,褐色脂肪の脂質,エネルギー代謝制御
血管,37:135-139 2014
Author:神吉昭子,桜井敬之,河手久香,新藤優佳,植竹龍一,山内啓弘,五十嵐恭子,鳥山佑一,田中愛,劉甜,羨鮮,今井章,翟留玉,大和慎治,新藤隆行


アドレノメデュリン受容体システムの機能分化と,血管・リンパ管の制御機構
リンパ学,37:57-61 2014
Author:山内啓弘,桜井敬之,神吉昭子,新藤優佳,河手久香,五十嵐恭子,鳥山佑一,田中愛,劉甜,羨鮮,翟留玉,荒居琢磨,新藤隆行


血管,臓器恒常性を制御する生体内システム アドレノメデュリンとその受容体活性調節システムの病態生理学的意義
日本薬理学雑誌,143:232-235 2014
Author:新藤隆行,桜井敬之,神吉昭子,新藤優佳,河手久香,小山晃英


Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
PLoS ONE,9(2):e87667 2014
Author:Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T


The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Xenotransplantation,21:291-300 2014
Author:Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S


Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia
Peptides,62:21-31 2014
Author:Igarashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T


A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
BMC Biotechnol,14:69 2014
Author:Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T


Functional differentiation of RAMP2 and RAMP3 in their regulation of vascular system
J Mol Cell Cardiol,77:73-85 2014
Author:Yamauchi A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Igarashi K, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T


Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection
PLoS One,9:e87667 2014
Author:Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T


Regulation of Adrenomedullin and its Family Peptide by RAMP System - Lessons from Genetically Engineered Mice
CURRENT PROTEIN & PEPTIDE SCIENCE,14(5):347-357 2013
Author:Shindo, T; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Shimoyama, N; Iinuma, N; Arai, T; Miyagawa, S


Vascular endothelial adrenomedullin-RAMP2 system is essential for vascular integrity and organ homeostasis
Circulation.,127(7):842-853 2013
Author:Koyama T, Ochoa-Callejero L, Sakurai T ,Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Arai T, Yoshizawa T, Iesato Y, Lei Y, Uetake R, Okimura A, Yamauchi A, Tanaka M, Igarashi K, Toriyama Y, Kawate H, Adams RH, Kawakami H, Mochizuki N, Martínez A, Shindo T


Novel regulation of cardiac metabolism and homeostasis by the adrenomedullin-receptor activity-modifying protein 2 system
Hypertension,61(2):341-351 2013
Author:Yoshizawa T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Nakada T, Kashihara T, Yamada M, Kawakami H, Nakanishi H, Taguchi R, Nakanishi T, Akazawa H, Shindo T.


Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury
J Mol Cell Cardiol.,59:55-66 2013
Author:Yang L, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T..


Adrenomedullin-RAMP2 system is crucially involved in retinal angiogenesis.
Am J Pathol.,182(6):2380-2390 2013
Author:Iesato Y, Toriyama Y, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Igarashi K, Murata T, Shindo T


Regulation of adrenomedullin and its family peptide by RAMP system--lessons from genetically engineered mice
Curr Protein Pept Sci.,14(5):347-357 2013
Author:Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Shimoyama N, Iinuma N, Arai T, Miyagawa S.


Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Biotechnol J.,8(11):1355-1361 2013
Author:Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S


Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression
Biology,2:341-355 2013
Author:Sato M Akasaka E Saitoh I Ohtsuka M Nakamura S Sakurai T, Watanabe S


Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study
Advances in Stem Cells,2013(2013):ID 208514 2013
Author:Sato M Kubota N Inada E Saitoh I Ohtsuka M Nakamura S Sakurai T Watanabe S


A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates
J. Biomedical Science and Engineering,5:406-408 2012
Author:Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S


Induction of LYVE-1/stabilin-2 -positive liver sinusoidal endothelial-like cells from embryoid bodies by modulation of adrenomedullin-RAMP2 signaling.
Peptides,32:1855-1865 2011
Author:Arai T, Sakurai T, Kamiyoshi A, Shindo Y, Iinuma N, Iesato Y, Koyama T, Yoshizawa T, Uetake R, Yamauchi A, Yang L, Kawate H, Ogawa S, Kobayashi A, Miyagawa S, Shindo T


Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression
N Biotechnol,28:312-319 2011
Author:Watanabe S; Nakamura S; Sakurai T; Akasaka K; Sato M


A novel glycosylation signal regulates transforming growth factor b receptors as evidenced by endo-b-galactosidase C expression in rodent cells
Glycobiology,21:486-492 2011
Author:Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Sato M


Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver
PEPTIDES,31(5):865-871 2010
Author:Iinuma, N; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Arai, T; Yoshizawa, T; Koyama, T; Uetake, R; Kawate, H; Muto, S; Tagawa, Y Miyagawa, S; Shindo, T


Development of CRTEIL and CETRIZ, Cre-loxP-based system, whichi allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
J Biomed Biotechnol,2009(2009):985140 2009
Author:Ohtsuka M; Warita T; Sakurai T; Watanabe S; Inoko H; Sato M


Endogenous alpha-calcitonin gene-related peptide mitigates liver fibrosis in chronic hepatitis induced by repeated administration of concanavalin A.
Liver Int,29(5):642-649 2009
Author:Kamiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Iinuma N; Kawate, H; Yoshizawa T; Koyama T; Muto, S; Shindo, T


Production and characterization of transgenic mice systemically expressing endo-beta-galactosidase C.
Glycobiology,18(1):9-19 2008
Author:Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Yokomine TA; Sato M


The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity.
J Clin Invest,118(1):29-39 2008
Author:Ichikawa-Shindo Y; Sakurai T; Kamiyoshi A; Kawate H et al.,


Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution
Transgenic Res,17(1):149-155 2008
Author:Sakurai T; Kamiyoshi A; Watanabe S; Sato M: Shindo T


Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,364(1):14-19 2007
Author:Nakanishi T; Kumagai S; Kimura M; Watanabe H; Sakurai T; Kimura M;Kashiwabara S; Baba T


Testatin transgenic and knockout mice exhibit normal sex-differentiation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,341(2):369-375 2006
Author:Hasegawa, K; Chuma, S; Tada, T; Sakurai, T; Tamura, M; Suemori, H; Nakatsuji, N


Folliculogenesis following syngeneic transplantation of young murine ovaries into the testes.
Reprod Med Bio,5(1):71-77 2006
Author:Sato M: Sakurai T; Kiryu K; Takeda M; Yasuoka Y


Strain difference in tolerance to low-temperature treatment of fertilized mouse oocytes.
Reprod Med Bio,5(1):43-50 2006
Author:Sakurai T; Watanabe S; Sato M: Kimura M


Azelnidipine, a long-acting dihydropyridine calcium channel blocker, suppresses the development of cardiac hypertrophy and fibrosis in adrenomedullin knock out mice.
CIRCULATION,114(18):13-13 2006
Author:Shindo, T; Niu, P; Kamiyoshi, A; Sakurai, T; Ichikawa, Y; Iinuma, N; Kawate, H


Preparationof enzymatically active human Myc tagged NCre recombinase exhibiting immunoreactivity with anti Myc antibody
Mol Reprod Dev,73(11):1345-1352 2006
Author:Watanabe S; Honnma D; Furusawa T; Sakurai T; Sato M


Endogenous alpha CGRP protects against concanavalin A-induced hepatitis in mice
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,343(1):152-158 2006
Author:Kainiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Fukuchi, J; Kawate, H; Muto, S; Tagawa, Y; Shindo, T


Temporal developmental arrest after storage of fertilized mouse eggs at 4oC: Effects on embryonic development, maternal mRNA processing and cell cycle
MORECULAR HUMAN REPRODUCTION,11(5):325-333 2005
Author:Sakurai T; Sato M: Kimura M


Divers patterns of poiy(A) tail elongation and shortning of murinematernal mRNAs from fully-grown oocyte to 2-cell embryo stages
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,336(4):1181-1189 2005
Author:Sakurai T; Sato M: Kimura M


A novel method for constructing murine cDNA library enriched with maternal mRNAs exhibiting de novo independent post-fertilization polyadenylation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,327(3):688-699 2005
Author:Sakurai T; Sato M: Kimura M


MISC
心血管系におけるadrenomedullin-RAMP2系の病態生理学的意義
心臓,45(12):1490-1495 2013
Author:新藤隆行、桜井敬之、神吉昭子、市川優佳

特許等知的財産
登録済特許
トランスジェニック動物の作製方法、トランスジェニック動物
DNAフラグメント及び医薬組成物
受精前の卵細胞、受精卵、および標的遺伝子の改変方法
研究費
受託研究
2015 - 2016 , 生理活性因子の情報制御システムに基づく、革新的な医薬品の創出, AMED-CREST
2013 - 2014 , 受容体活性調節タンパクRAMPを標的とした創薬 科学技術振興機構 研究成果最適展開支援プログラム

科学研究費補助金(研究代表者)
2019 - 2021 , 体系的複数同時ゲノム編集法を応用したRAMP1、2、3の機能分化と相互作用の解明 , 基盤研究 (C)
2016 - 2017 , 「母性Cas9」による多遺伝子編集マウス開発と多因子性疾患の解明 , 萌芽研究
2014 - 2017 , 次世代遺伝子改変技術を応用した炎症性腸疾患におけるR2・R3の機能連関の解明 , 基盤研究(B)
2014 - 2016 , 体系的・多遺伝子同時改変マウス作製法開発によるアドレノメデュリンRAMP機能解明 , 萌芽研究
2011 - 2014 , アドレノメデュリン-RAMP2・RAMP3システムの破綻が惹起する炎症病態の解明 , 基盤研究(C)
2008 - 2010 , 多機能に関わるアドレノメデュリンーRAMPシステムの発生工学的病態解析と治療検討 , 基盤研究(C)
2006 - 2007 , キメラマウスによる多機能ペプチド、アドレノメデュリンと関連因子の全組織包括的解析 , 基盤研究(C)
2004 - 2005 , マウス受精卵で転写を伴わないポリA鎖伸長を示す母性RNA群の多様性と生物学的意義 , 基盤研究(C)
2000 - 2001 , マウス受精卵における時期特異的ポリ(A)鎖伸長母性RNA群の系統的解明 , 奨励研究(A)

科学研究費補助金(研究分担者)
2021 - 2023 , 受容体活性調節システムによる生体内恒常性制御機構の解明と創薬への展開 , 基盤研究(B)
2020 - 2020 , 血管およびリンパ管の恒常性制御は、癌転移抑制法となりうるか? , 挑戦的研究(萌芽)
2018 - 2021 , 癌特異的代謝を介し増悪化に寄与するGPCR OR7C1の作用機序解明と治療応用 , 基盤研究(C)
2015 - 2015 , 創薬研究の加速に向けた、次世代遺伝子編集技術の開発 JST研究成果展開事業 マッチングプランナープログラム「探索試験」
2014 - 2017 , 臓器間連携と恒常性を司る、新しい生体内情報制御システムの解明と応用展開 , 基盤研究(B)
2009 - 2011 , 創薬シーズ開発の効率化に向けた次世代疾患モデルマウスの迅速作製技術開発 , JST A-Step 研究成果最適化支援事業
2008 - 2010 , RAMPによる代謝、血管制御メカニズムの解明と、代謝異常、血管合併症治療への展開 , 基盤研究(B)
2003 - 2005 , マウス初期胚における細胞質RNAの動的変化の重要性の解明 , 基盤研究(B)
2001 - 2001 , 受精後の初期発生プログラムに連動するポリA鎖伸長母性RNAの網羅的アプローチ , 特定領域研究(C)
2000 - 2000 , マウス初期胚母性RNAの時期特異的ポリ(A)鎖伸長の意義 , 特定領域研究(A)
1996 - 1997 , マウス着床前初期胚における遺伝子発現の総合的解析 , 国際学術

その他公的資金
2023 - 2023 , 信州医学振興会
2021 - 2021 , アイバワークス医学研究者助成
2017 - 2017 , 信州医学振興会
2011 - 2011 , 内藤記念科学奨励金 血管統合機構の解明と治療応用
2009 - 2009 , アストラゼネカグラントリサーチ2009
2006 - 2006 , 三井生命医学研究助成 アドレノメデユリンーRAMP2システムを標的とした、メタポリックシンドローム、動脈硬化症の新規治療法開発
2006 - 2006 , アストラゼネカグラントリサーチ2006

教育活動実績

授業等
医科学研究概論
分子発生学2006-2012
臓器生着学2006-2012