Sakurai Takayuki

Academic Assembly School of Medicine and Health Sciences Institute of Medicine

Interdisciplinary Cluster for Cutting Edge Research Institute for Biomedical Sciences 

Associate Professor 

Degree

  • Doctor of Science, Grad School Yokohama City Univ
  • Master of Science, Grad School Shizuoka Univ

Research Keyword

    Developmental engineering, Genome Editing, Developmental biology, Transgenic animal

Field Of Study

  • Developmental biology
  • Developmental Biothechnology
  • Cardiovascular Biology
  • Genome Editing

Mail Address

    tsakurai★shinshu-u.ac.jp

Career

  • 2019
    Associate Professor, Department of Life Innovation, Institute for Biomedical Sciences, Shinshu University
  • 2008
    Associate Professor Grad School Med Shinshu Univ
  • 2005 - 2008
    Senior Assistant Professor Grad School Med Shinshu Univ
  • 1999 - 2005
    Assistant Professor Schooi of Med Tokai Unicersity

Educational Background

  • 1995, Grad School Yokohama City Univ
  • 1992, Grad School Shizuoka Univ
  • 1990, Shizuoka Univ

Paper

  • An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility
    Negishi K, Aizawa K, Shindo T, Suzuki T, Sakurai T, Saito Y, Miyakawa Y, Tanokura M, Kataoka Y, Maeda M, Shota Tomida, Hiroyuki Morita, Takeda N, Komuro I, Kario K, Nagai R, Imai Y
    Sci Rep, 12(1), 8844, 2022
  • Receptor activity modifying protein RAMP sub-isoforms and their functional differentiation, which regulates functional diversity of adrenomedullin
    Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Sakurai T
    Biology, 11(5), 788, 2022
  • Adrenomedullin-RAMP2 system ameliorates subretinal fibrosis by suppressing epithelial-mesenchymal transition in age-related macular degeneration
    Tanaka M, Kakihara S, Hirabayashi K, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Cui N, Wei Y, Zhao Y, Aruga K, Yamauchi A, Murata T, Shindo T
    Am J Pathol, 191(4), 652-668, 2021
  • Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress
    Cui N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Tanaka M, Wei Y, Kakihara S, Zhao Y, Aruga K, Kawagishi H, Nakada T, Yamada M, Shindo T
    Endocrinology, 162(3), 1-20, 2021
  • Adrenomedullin Ameliorates Pulmonary Fibrosis by Regulating TGF-ß-Smads Signaling and Myofibroblast Differentiation
    Wei Y, Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Cui N, Kakihara S, Zhao, Y, Aruga K, Sanjo H, Shindo T
    Endocrinology, 162(8), 1-22, 2021
  • Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing
    Sakurai T, Shindo T
    STAR Protoc, 2(2), 2021
  • Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
    Sakurai, T.; Kamiyoshi, A.; Kawate, H.; Watanabe, S.; Sato, M.; Shindo, T.
    Sci.Rep, 10(1), 1091, 2020
  • Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts.
    Dai, K.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Matsui, S.; Shindo, T.
    Oncogene, 39(10), 1914-1930, 2020
  • Development of a Novel Model of Central Retinal Vascular Occlusion and the Therapeutic Potential of the Adrenomedullin-Receptor Activity-Modifying Protein 2 System.
    Hirabayashi, K.; Tanaka, M.; Imai, A.; Toriyama, Y.; Iesato, Y.; Sakurai, T.; Kamiyoshi, A.; Ichikawa-Shindo, Y.; Kawate, H.; Tanaka, M.; Dai, K.; Cui, N.; Wei, Y.; Nakamura, K.; Iida, S.; Matsui, S.; Yamauchi, A.; Murata, T.; Shindo, T.
    Am J Pathol.,, 189, 449-466, 2019
  • Endogenous Calcitonin Gene-Related Peptide Deficiency Exacerbates Postoperative Lymphedema by Suppressing Lymphatic Capillary Formation and M2 Macrophage Accumulation.
    Matsui, S.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Dai, K.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Nakamura, K.; Yamauchi, A.; Ishida, K.; Tanaka, S.; Kawamata, M.; Shindo, T.
    Am J Pathol.,, 189, 2487-2502, 2019
  • Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice.
    Yamauchi, A.; Kamiyoshi, A.; Sakurai, T.; Miyazaki, H.; Hirano, E.; Lim, HS.; Kaku, T.; Shindo, T.
    Heliyon, 5, e02655, 2019
  • Regulation of cardiovascular development and homeostasis by the adrenomedullin-RAMP system
    Shindo T, Tanaka M, Kamiyoshi, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Sakurai T
    PEPTIDES, 111, 55-61, 2019
  • Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
    Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.
    Sci Rep, 9(1), 9923, 2019
  • Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid-gestational murine fetuses.
    Nakamura S, Ishihara M, Ando N, Watanabe S, Sakurai T, Sato M
    IUBMB Life, 71(7), 835-844, 2019
  • RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders
    Liu T, Kamiyoshi A, Tanaka M, Iida S, Sakurai T, Ichikawa-Shindo Y, Kawate H, Hirabayashi K, Dai K, Cui N, Tanaka M, Wei Y, Nakamura K, Matsui S, Yamauchi A, Shindo T
    PEPTIDES, 110, 10-18, 2018
  • In vivo genome editing targeted towards the female reproductive system.
    Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Gurumurthy CB
    Arch Pharm Res., 41(9), 898-910, 2018
  • The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.
    Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M.
    Int J Mol Sci., 19(4), E1075, 2018
  • Endogenous calcitonin gene-related peptide suppresses ischemic brain injuries and progression of cognitive decline.
    Zhai L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Xian X, Hirabayashi K, Dai K, Cui N, Tanimura K, Liu T, Wei Y, Tanaka M, Tomiyama H, Yamauchi A, Igarashi K, Shindo T.
    J Hypertens, 36(4), 876-891, 2018
  • Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
    Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
    Theriogenology, 108, 29-38, 2018WebofScienceリポジトリ電子ジャーナル
  • Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy.
    Imai A, Toriyama Y, Iesato Y, Hirabayashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Liu T, Xian X, Zhai L, Dai K, Tanimura K, Liu T, Cui N, Yamauchi A, Murata T, Shindo T.
    Am J Pathol., 187(5), 999-1015, 2017
  • Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
    Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
    Endocrinology., 158(5), 1359-1372, 2017
  • Endogenous Calcitonin Gene-Related Peptide Regulates Lipid Metabolism and Energy Homeostasis in Male Mice.
    Liu T, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Yang L, Tanaka M, Xian X, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
    Endocrinology., 158(5), 1194-1206, 2017
  • Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
    Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.
    Int J Mol Sci., 18(12), E2610, 2017WebofScienceリポジトリ電子ジャーナル
  • Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
    Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K
    Int.J.Mol.Sci, 16, 17838-17856, 2016
  • The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis
    Tanaka M, Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Liu T, Xian X, Imai A, Zhai L, Hirabayashi K, Owa S, Yamauchi A, Igarashi K, Taniguchi S, Shindo T
    Cardiovasc.Res, 111, 398-409, 2016
  • The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
    Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K
    17, E1424, 2016
  • Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury
    Ayuzawa N, Nagase M, Ueda K, Nishimoto M, Kawarazaki W, Marumo T, Aiba A, Sakurai T, Shindo T, Fujita T
    Hypertension, 67, 99-106, 2016
  • Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques
    Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K
    Sci.Rep, 6, srep35861, 2016
  • A non-inheritable maternal Cas9-based multiple-gene editing system in mice
    Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T
    Sci.Rep, 6, srep20011, 2016
  • Pathophysiological Function of Endogenous Calcitonin Gene-Related Peptide in Ocular Vascular Diseases
    Toriyama Y, Iesato Y, Imai A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Igarashi K, Tanaka M, Liu T, Xian X, Zhai L, Owa S, Murata T, Shindo T
    Am J Pathol, 185, 1783-1794, 2015
  • Adrenomedullin-RAMP2 System in Vascular Endothelial Cells
    Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Shindo T
    J Atheroscler Thromb, 22, 647-653, 2015
  • A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
    Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S
    Biotechnol J, 10, 143-153, 2015
  • Generation of α-1,3-Galactosyltransferase-Deficient Porcine Embryonic Fibroblasts by CRISPR/Cas9-Mediated Knock-in of a Small Mutated Sequence and a Targeted Toxin-Based Selection System
    Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S.
    Reprod Domest Anim, 50, 872-880, 2015
  • Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
    Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T
    PLOS ONE, 9, e87667, 2014
  • Functional differentiation of RAMP2 and RAMP3 in their regulation of vascular system
    Yamauchi A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Igarashi K, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T
    J Mol Cell Cardiol, 77, 73-85, 2014
  • A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
    Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T
    BMC Biotechnol, 14, 69, 2014
  • Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia
    Igarashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T
    PEPTIDES, 62, 21-31, 2014
  • The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
    Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    Xenotransplantation, 21, 291-300, 2014
  • Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
    Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T
    PLOS ONE, 9(2), e87667, 2014
  • The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
    Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    Xenotransplantation, 21, 291-300, 2014
  • Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
    Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    Biotechnol J, 8, 1355-1361, 2014
  • Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression
    Sato M Akasaka E Saitoh I Ohtsuka M Nakamura S Sakurai T, Watanabe S
    Biology, 2, 341-355, 2013
  • Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury
    Yang L, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T..
    J Mol Cell Cardiol., 59, 55-66, 2013
  • Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study
    Sato M Kubota N Inada E Saitoh I Ohtsuka M Nakamura S Sakurai T Watanabe S
    Advances in Stem Cells, 2013(2013), ID 208514, 2013
  • Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
    Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    Biotechnol J., 8(11), 1355-1361, 2013
  • Regulation of adrenomedullin and its family peptide by RAMP system--lessons from genetically engineered mice
    Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Shimoyama N, Iinuma N, Arai T, Miyagawa S.
    Curr Protein Pept Sci., 14(5), 347-357, 2013
  • Adrenomedullin-RAMP2 system is crucially involved in retinal angiogenesis.
    Iesato Y, Toriyama Y, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Igarashi K, Murata T, Shindo T
    Am J Pathol., 182(6), 2380-2390, 2013
  • Novel Regulation of Cardiac Metabolism and Homeostasis by the Adrenomedullin-Receptor Activity-Modifying Protein 2 System
    Yoshizawa T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Nakada T, Kashihara T, Yamada M, Kawakami H, Nakanishi H, Taguchi R, Nakanishi T, Akazawa H, Shindo T.
    Hypertension, 61(2), 341-351, 2013
  • Vascular Endothelial Adrenomedullin-RAMP2 System Is Essential for Vascular Integrity and Organ Homeostasis
    Koyama T, Ochoa-Callejero L, Sakurai T ,Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Arai T, Yoshizawa T, Iesato Y, Lei Y, Uetake R, Okimura A, Yamauchi A, Tanaka M, Igarashi K, Toriyama Y, Kawate H, Adams RH, Kawakami H, Mochizuki N, Martínez A, Shindo T
    Circulation., 127(7), 842-853, 2013
  • Regulation of Adrenomedullin and its Family Peptide by RAMP System - Lessons from Genetically Engineered Mice
    Shindo, T; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Shimoyama, N; Iinuma, N; Arai, T; Miyagawa, S
    CURRENT PROTEIN & PEPTIDE SCIENCE, 14(5), 347-357, 2013WebofScienceリポジトリ電子ジャーナル
  • A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates
    Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
    J. Biomedical Science and Engineering, 5, 406-408, 2012
  • A novel glycosylation signal regulates transforming growth factor b receptors as evidenced by endo-b-galactosidase C expression in rodent cells
    Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Sato M
    GLYCOBIOLOGY, 21, 486-492, 2011
  • Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression
    Watanabe S; Nakamura S; Sakurai T; Akasaka K; Sato M
    N Biotechnol, 28, 312-319, 2011
  • Induction of LYVE-1/stabilin-2 -positive liver sinusoidal endothelial-like cells from embryoid bodies by modulation of adrenomedullin-RAMP2 signaling.
    Arai T, Sakurai T, Kamiyoshi A, Shindo Y, Iinuma N, Iesato Y, Koyama T, Yoshizawa T, Uetake R, Yamauchi A, Yang L, Kawate H, Ogawa S, Kobayashi A, Miyagawa S, Shindo T
    PEPTIDES, 32, 1855-1865, 2011WebofScienceリポジトリ電子ジャーナル
  • Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver
    Iinuma, N; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Arai, T; Yoshizawa, T; Koyama, T; Uetake, R; Kawate, H; Muto, S; Tagawa, Y Miyagawa, S; Shindo, T
    PEPTIDES, 31(5), 865-871, 2010WebofScienceリポジトリ電子ジャーナル
  • Endogenous alpha-calcitonin gene-related peptide mitigates liver fibrosis in chronic hepatitis induced by repeated administration of concanavalin A.
    Kamiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Iinuma N; Kawate, H; Yoshizawa T; Koyama T; Muto, S; Shindo, T
    Liver Int, 29(5), 642-649, 2009
  • Development of CRTEIL and CETRIZ, Cre-loxP-based system, whichi allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
    Ohtsuka M; Warita T; Sakurai T; Watanabe S; Inoko H; Sato M
    J Biomed Biotechnol, 2009(2009), 985140, 2009
  • Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution
    Sakurai T; Kamiyoshi A; Watanabe S; Sato M: Shindo T
    Transgenic Res, 17(1), 149-155, 2008
  • The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity.
    Ichikawa-Shindo Y; Sakurai T; Kamiyoshi A; Kawate H et al.,
    J Clin Invest, 118(1), 29-39, 2008
  • Production and characterization of transgenic mice systemically expressing endo-beta-galactosidase C.
    Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Yokomine TA; Sato M
    GLYCOBIOLOGY, 18(1), 9-19, 2008
  • Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
    Nakanishi T; Kumagai S; Kimura M; Watanabe H; Sakurai T; Kimura M;Kashiwabara S; Baba T
    Biochemical and biophysical research communications, 364(1), 14-19, 2007
  • Preparationof enzymatically active human Myc tagged NCre recombinase exhibiting immunoreactivity with anti Myc antibody
    Watanabe S; Honnma D; Furusawa T; Sakurai T; Sato M
    Mol Reprod Dev, 73(11), 1345-1352, 2006
  • Strain difference in tolerance to low-temperature treatment of fertilized mouse oocytes.
    Sakurai T; Watanabe S; Sato M: Kimura M
    Reprod Med Bio, 5(1), 43-50, 2006
  • Folliculogenesis following syngeneic transplantation of young murine ovaries into the testes.
    Sato M: Sakurai T; Kiryu K; Takeda M; Yasuoka Y
    Reprod Med Bio, 5(1), 71-77, 2006
  • Endogenous alpha CGRP protects against concanavalin A-induced hepatitis in mice
    Kainiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Fukuchi, J; Kawate, H; Muto, S; Tagawa, Y; Shindo, T
    Biochemical and biophysical research communications, 343(1), 152-158, 2006WebofScience
  • Azelnidipine, a long-acting dihydropyridine calcium channel blocker, suppresses the development of cardiac hypertrophy and fibrosis in adrenomedullin knock out mice.
    Shindo, T; Niu, P; Kamiyoshi, A; Sakurai, T; Ichikawa, Y; Iinuma, N; Kawate, H
    Circulation, 114(18), 13-13, 2006WebofScience
  • Testatin transgenic and knockout mice exhibit normal sex-differentiation
    Hasegawa, K; Chuma, S; Tada, T; Sakurai, T; Tamura, M; Suemori, H; Nakatsuji, N
    Biochemical and biophysical research communications, 341(2), 369-375, 2006WebofScience
  • A novel method for constructing murine cDNA library enriched with maternal mRNAs exhibiting de novo independent post-fertilization polyadenylation
    Sakurai T; Sato M: Kimura M
    Biochemical and biophysical research communications, 327(3), 688-699, 2005
  • Temporal developmental arrest after storage of fertilized mouse eggs at 4oC: Effects on embryonic development, maternal mRNA processing and cell cycle
    Sakurai T; Sato M: Kimura M
    MORECULAR HUMAN REPRODUCTION, 11(5), 325-333, 2005
  • Divers patterns of poiy(A) tail elongation and shortning of murinematernal mRNAs from fully-grown oocyte to 2-cell embryo stages
    Sakurai T; Sato M: Kimura M
    Biochemical and biophysical research communications, 336(4), 1181-1189, 2005, Refereed

Books and other publications

  • Microinjection: Methods and Protocols Methods in Molecular Biology
    Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B Gurumurthy, Masahiro Sato, Takayuki Shindo, Joint work, Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line
    Springer Nature, Chapter 15 2019, Refereed
  • Results and Problems in Cell Differentiation 63
    Takayuki Sakurai, Takayuki Shindo, Masahiro Sato, Joint work, Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals
    Springer, Chapter 21 2017, Refereed
  • 細胞工学 35 1
    新藤隆行、桜井敬之、神吉昭子、市川優佳、田中愛、小山晃英, アドレノメデュリン-RAMP2シグナル
    33-38 2016
  • 医学のあゆみ 223 13
    新藤隆行 桜井敬之, アドレノメデュリンによる血管形成の調節-遺伝子操作動物における検討ー
    医歯薬出版, 1063-1068 2007
  • RNAi A GUIDE TO GENE SILENCING
    桜井敬之 木村穣 (訳), マウスの卵母細胞と着床前胚に対するRNAi実験入門
    メディカルサイエンスインターナショナル, 303-333 2004

Courses

  • Introduction to Medical Science
    Shinshu University
  • Molecular Embryology
    Shinshu University
  • Graft Adaptation
    Shinshu University

Affiliated academic society

  • The Molecular Biology Society of Japan
  • The Japanese Society for Regenerative Medicine
  • The Japanese Society for Genome Editing

Research Themes

  • 癌特異的代謝を介し増悪化に寄与するGPCR OR7C1の作用機序解明と治療応用
    科学研究費補助金, 基盤研究(C)
    01 Apr. 2018 - 31 Mar. 2021
  • 体系的複数同時ゲノム編集法を応用したRAMP1、2、3の機能分化と相互作用の解明
    科学研究費補助金, 基盤研究 (C)
  • キメラマウスによる多機能ペプチド、アドレノメデュリンと関連因子の全組織包括的解析
    科学研究費補助金, 基盤研究(C)
  • 多機能に関わるアドレノメデュリンーRAMPシステムの発生工学的病態解析と治療検討
    科学研究費補助金, 基盤研究(C)
  • マウス受精卵で転写を伴わないポリA鎖伸長を示す母性RNA群の多様性と生物学的意義
    科学研究費補助金, 基盤研究(C)
  • マウス受精卵における時期特異的ポリ(A)鎖伸長母性RNA群の系統的解明
    科学研究費補助金, 奨励研究(A)
  • アドレノメデュリン-RAMP2・RAMP3システムの破綻が惹起する炎症病態の解明
    科学研究費補助金, 基盤研究(C)
  • 体系的・多遺伝子同時改変マウス作製法開発によるアドレノメデュリンRAMP機能解明
    科学研究費補助金, 萌芽研究
  • 次世代遺伝子改変技術を応用した炎症性腸疾患におけるR2・R3の機能連関の解明
    科学研究費補助金, 基盤研究(B)
  • 「母性Cas9」による多遺伝子編集マウス開発と多因子性疾患の解明
    科学研究費補助金, 萌芽研究
  • 受容体活性調節タンパクRAMPを標的とした創薬 科学技術振興機構 研究成果最適展開支援プログラム
  • 三井生命医学研究助成 アドレノメデユリンーRAMP2システムを標的とした、メタポリックシンドローム、動脈硬化症の新規治療法開発
  • 内藤記念科学奨励金 血管統合機構の解明と治療応用
  • 信州医学振興会
  • アイバワークス医学研究者助成
  • 生理活性因子の情報制御システムに基づく、革新的な医薬品の創出, AMED-CREST
  • RAMPによる代謝、血管制御メカニズムの解明と、代謝異常、血管合併症治療への展開
    科学研究費補助金, 基盤研究(B)
  • マウス初期胚における細胞質RNAの動的変化の重要性の解明
    科学研究費補助金, 基盤研究(B)
  • 受精後の初期発生プログラムに連動するポリA鎖伸長母性RNAの網羅的アプローチ
    科学研究費補助金, 特定領域研究(C)
  • マウス初期胚母性RNAの時期特異的ポリ(A)鎖伸長の意義
    科学研究費補助金, 特定領域研究(A)
  • マウス着床前初期胚における遺伝子発現の総合的解析
    科学研究費補助金, 国際学術
  • 創薬シーズ開発の効率化に向けた次世代疾患モデルマウスの迅速作製技術開発
    科学研究費補助金, JST A-Step 研究成果最適化支援事業
  • 臓器間連携と恒常性を司る、新しい生体内情報制御システムの解明と応用展開
    科学研究費補助金, 基盤研究(B)
  • 創薬研究の加速に向けた、次世代遺伝子編集技術の開発 JST研究成果展開事業 マッチングプランナープログラム「探索試験」
    科学研究費補助金
  • 受容体活性調節システムによる生体内恒常性制御機構の解明と創薬への展開
    科学研究費補助金, 基盤研究(B)
  • 血管およびリンパ管の恒常性制御は、癌転移抑制法となりうるか?
    科学研究費補助金, 挑戦的研究(萌芽)
  • アストラゼネカグラントリサーチ2006
  • アストラゼネカグラントリサーチ2009
  • Generation and characterization of new mouse models of human diseases
  • Development of new technologies needed for generating gene-modified animal
  • Understanding of Adrenomedullin/Ramps functions that are involved in cardiovascular diseases and inflammation
  • 信州医学振興会

Industrial Property Rights

  • DNAフラグメント及び医薬組成物, 5758803, Patent right
    桜井敬之 新藤隆行
  • トランスジェニック動物の作製方法、トランスジェニック動物, 3694734, 特許公開2001-309736, 特許出願2000-354339, Patent right
    中辻憲夫、田村勝、黄振勇、桜井敬之、中馬新一郎、斉藤哲一郎
  • 受精前の卵細胞、受精卵、および標的遺伝子の改変方法, 6700306, Patent right
    桜井敬之 新藤隆行