ARAI, Ryoichi | Shinshu University Researcher Directory

Academic Organization	Academic Assembly　School of Science and Technology　Institute of Textile Science and Technology	TEL
Education and Research Organization	Interdisciplinary Cluster for Cutting Edge Research　Institute for Biomedical Sciences	FAX
Position	Associate Professor	Mail Address	rarai[at]shinshu-u.ac.jp
Address	3-15-1 Tokida, Ueda, Nagano 386-8567, JAPAN	Web site	http://fiber.shinshu-u.ac.jp/arai/

Profile

Assigned Class: Faculty of Textile Science and Technology　Department of Applied Biology

Research Field: Protein engineering, Protein design, Structural biology, Biotechnology, Synthetic biology
Keywords:X-ray crystallography , Protein design , Fusion/Chimeric protein , Engineered protein

Current Subject: Design and construction of protein nanobuilding block complexes

Design and development of artificial proteins

Construction and application of engineered proteins and fusion/chimeric proteins

Protein X-ray crystallography

Academic Societies: Academic Societies
Protein Science Society of Japan
The Society for Biotechnology, Japan
The Molecular Biology Society of Japan
Japanese Society of Enzyme Engineering
Division of Biofunctional Chemistry, the Chemical Society of Japan
Japanese Society for Cell Synthesis Research

Academic Background: Graduate School
The University of Tokyo , (Department of Chemistry and Biotechnology , Graduate School of Engineering) , 2001
The University of Tokyo , (Department of Chemistry and Biotechnology , Graduate School of Engineering) , 1998

College
The University of Tokyo , (School of Engineering) , 1996

Degree
Ph.D. , University of Tokyo

Awards: 2015 , Encouraging prize of Japanese society of enzyme engineering , "Study on structural analysis of a de novo protein and its application to construction of nanostructures"
2013 , Leave a Nest Grant Direct Detect Award
2011 , PDB40 Travel Award , Protein Data Bank 40th Anniversary Symposium
2011 , Protein Science Young Investigator Travel Grant / The Protein Society Finn Wold Travel Award , IX European Symposium of The Protein Society
2001 , Ishii Science Promotion Award, General Research Promotion Foundation
1996 , Award for graduate student, The Society of Chemical Engineers, Japan

Research Career: Research Career
2019- , Department of Biomolecular Innovation, Institute for Biomedical Sciences, ICCER, Shinshu University
2016- , Assistant Professor, School of Science and Technology, Academic Assembly, Shinshu University
2017-2021 , Journal of Bioscience and Bioengineering, Editor
2016-2019 , Department Head, Department of Supramolecular Complexes, Research Center for Fungal and Microbial Dynamism, Shinshu University
2014-2016 , Assistant Professor, School of Science and Technology, Academic Assembly, Shinshu University
2013-2018 , Visiting Researcher, Division of Structural and Synthetic Biology, Center for Life Science and Technologies, RIKEN
2012-2014 , Assistant Professor, Faculty of Textile Science and Technology, Shinshu University
2007-2012 , Assistant Professor, International Young Researchers Empowerment Center, Shinshu University
2006-2007 , JSPS Postdoctoral Fellow for Research Abroad (Prof. Michael H. Hecht Lab, Princeton University, NJ, USA)
2004-2006 , Research Scientist, Genomic Sciences Center, RIKEN Yokohama Institute (Dr. Shigeyuki Yokoyama Gr.)
2001-2004 , Research Associate, Genomic Sciences Center, RIKEN Yokohama Institute (Dr. Shigeyuki Yokoyama Gr.)
1998-2001 , JSPS Research Fellow (DC1)

Overseas Education
2019-2019 , Visiting Scholar, Institute for Protein Design, University of Washington, WA, USA (Prof. David Baker Lab.)
2006-2007 , Research fellow, Department of Chemistry, Princeton University, NJ, USA (Prof. Michael H. Hecht Lab.)

Research

Books, Articles, etc.: Books
Linkers in Biomacromolecules Chapter 8 "Design of helical linkers for fusion proteins and protein-based nanostructures", Methods in Enzymology 647 , 209-230
Elsevier 2021
Author:Ryoichi Arai

Characterization of underwater silk proteins from caddisfly larva, Stenopsyche marmorata, Biotechnology of Silk: Biologically inspired systems Volume 5 , Ch. 6, pp. 107-122
Springer, New York 2013
Author:Ohkawa, K., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe , K.

Articles
Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,145(1):216-223 2023
Author:Ohara, Naoya; Kawakami, Norifumi; Arai, Ryoichi; Adachi, Naruhiko; Moriya, Toshio; Kawasaki, Masato; Miyamoto, Kenji;

Production of pentaglycine-fused proteins using Escherichia coli expression system without in vitro peptidase treatment
PROTEIN EXPRESSION AND PURIFICATION,194:106068 2022(Jun.)
Author:Sakono, Masafumi; Oshima, Tatsuki; Iwakawa, Takako; Arai, Ryoichi;
Keywords:Methionyl aminopeptidase; Peptide ligation; Polyglycine; Protein expression; Sortase A;

Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans
International Journal of Molecular Sciences,23(2):676 2022(Jan.)
Author:Shin Irumagawa, Keiko Hiemori, Sayoko Saito, Hiroaki Tateno, Ryoichi Arai

Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60
Chemical Communications,57(79):10226-10229 2021(Sep.)
Author:Junya Obata, Norifumi Kawakami, Akihisa Tsutsumi, Erika Nasu, Kenji Miyamoto, Masahide Kikkawa, Ryoichi Arai

Rational thermostabilisation of four-helix bundle dimeric de novo proteins
Scientific Reports,11:7526 2021(Apr. 06)
Author:Shin Irumagawa, Kaito Kobayashi, Yutaka Saito, Takeshi Miyata, Mitsuo Umetsu, Tomoshi Kameda & Ryoichi Arai

Hyperstable De Novo Protein with a Dimeric Bisecting Topology
ACS Synthetic Biology,9(2):254-259 2020
Author:Kimura, N., Mochizuki, K., Umezawa K., Hecht, M.H., Arai, R.

Lectin engineering: the possible and the actual
Interface Focus,9:20180068 2019
Author:Hirabayashi, J., Arai, R.

Design of hollow protein nanoparticles with modifiable interior and exterior surfaces
Angewandte Chemie International Edition,57:12400-12404 2018
Author:Norifumi Kawakami, Hiroki Kondo, Yuki Matsuzawa, Kaoru Hayasaka, Erika Nasu, Kenji Sasahara, Ryoichi Arai, Kenji Miyamoto

Self-assembling supramolecular nanostructures constructed from de novo extender protein nanobuilding blocks
ACS Synthetic Biology,7(5):1381-1394 2018
Author:Naoya Kobayashi, Kouichi Inano, Kenji Sasahara, Takaaki Sato, Keisuke Miyazawa, Takeshi Fukuma, Michael H Hecht, Chihong Song, Kazuyoshi Murata, and Ryoichi Arai

Hierarchical design of artificial proteins and complexes toward synthetic structural biology
Biophysical Reviews,10:391-410 2018
Author:Arai, R.

Comparative analysis of plant isochorismate synthases reveals structural mechanisms underlying their distinct biochemical properties
Bioscience Reports,38:BSR20171457 2018
Author:2. Yokoo, S., Inoue, S., Suzuki, N., Amakawa, N., Matsui, H., Nakagami, H., Takahashi, A., Arai R. and Katou, S.

Design and construction of self-assembling supramolecular protein complexes using artificial and fusion proteins as nanoscale building blocks
Current Opinion in Biotechnology,46:57–65 2017
Author:Kobayashi, N. and Arai, R.

Flight density of the aquatic insect fauna over the water surface in the middle reaches of the Shinano River, Japan, mainly among caddisflies (Trichoptera)
Zoosymposia,10:203–213 2016
Author:Hirabayashi, K.*, Ikutama, E., Ohkawa, K., Arai, R., Nomura, T., Tsukada, M., and Abe, K.

Characterization of silk gland ribosomes from a bivoltine caddisfly, Stenopsyche marmorata: translational suppression of a silk protein in cold conditions.
Biochemical and Biophysical Research Communications,469:210-215 2016
Author:Takaomi Nomura, Miho Ito, Mai Kanamori, Yuta　Shigeno,　Toshio Uchiumi, Ryoichi Arai, Masuhiro Tsukada, Kimio Hirabayashi, Kousaku Ohkawa

Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis
Acta Cryst. F,F71(12):1516–1520 2015(Dec.)
Author:Miruku Ueda, Makoto Shimosaka and Ryoichi Arai

Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
Biochem. Biophys. Res. Commun.,464(3):814–819 2015
Author:Xue Bai, Mayo Sakaguchi, Yuko Yamaguchi, Shiori Ishihara, Masuhiro Tsukada, Kimio Hirabayashi, Kousaku Ohkawa, Takaomi Nomura, Ryoichi Arai

Self-Assembling Nano-Architectures Created from a Protein Nano-Building Block Using an Intermolecularly Folded Dimeric De Novo Protein
J. Am. Chem. Soc.,137(35):11285–11293 2015
Author:Naoya Kobayashi, Keiichi Yanase, Takaaki Sato, Satoru Unzai, Michael H. Hecht, Ryoichi Arai

Crystal structure of ChiL, a chitinase from Chitiniphilus shinanonensis
Photon Factory Activity Report 2014,32(B):206 2015
Author:Ueda, M., Shimosaka, M., and Arai, R.

Crystal structure of Fab fragment of an anti-Osteocalcin C-Terminal peptide antibody KTM219
Photon Factory Activity Report 2014,32(B):205 2015
Author:Komatsu, M., Dong, J., Ueda, H., and Arai, R.

Crystal structure of glycine oxidase from Geobacillus kaustophilus
Photon Factory Activity Report 2014,32(B):204 2015
Author:Shiono, T., Nomura, T., Nishiya, Y., and Arai, R.

Intermolecular interactions and molecular dynamics in bovine serum albumin solutions studied by small angle X-ray scattering and dielectric relaxation spectroscopy.
J. Mol. Liq.,200(A):59-66 2014
Author:Yanase, K., Arai R., and Sato, T.

Functional characterization of FABP3, 5 and 7 gene variants identified in schizophrenia and autism spectrum disorder and mouse behavioral studies.
Hum. Mol. Genet.,23:6495-6511 2014
Author:Shimamoto C, Ohnishi T, Maekawa M, Watanabe A, Ohba H, Arai R, Iwayama Y,　Hisano Y, Toyota T, Toyoshima M, Suzuki K, Shirayama Y, Nakamura K, Mori N, Owada, Y, Kobayashi T, and Yoshikawa T

Crystal structure of glycine oxidase from Bacillus thuringiensis
Photon Factory Activity Report 2013,31(B):274 2014
Author:Ohishi, S., Shiono, T., Nishiya, Y., Nomura, T., and Arai, R.

バイナリーパターンデザインによるデノボタンパク質WA20のドメインスワップ二量体構造
酵素工学研究会誌,69:19-25 2013
Author:小林直也，*新井亮一

Chain conformational study on underwater silk protein from caddisfly, Stenopsyche marmorata: Implication of a fiber-forming mechanism
Adv. Mater. Res.,796:41706 2013
Author:Ohkawa, K., Hachisu, M., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe, K

Long-range periodic sequence of Stenopsyche marmorata cement/silk protein: Purification and biochemical characterization
Biofouling,29(4):357-367 2013
Author:Ohkawa, K., Miura, Y., Nomura, T., Arai, R., Abe, K., Tsukada M. and Hirabayashi, K.

Isolation of silk proteins from a caddisfly larva, Stenopsyche marmorata.
J. Fiber Bioeng. Informatics,5:125-137 2012
Author:Ohkawa, K., Miura, Y., Nomura, T., Arai, R., Abe, K., Tsukada M. and Hirabayashi, K.

Solution structure of IseA, an inhibitor protein of DL-endopeptidases from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop
J. Biol. Chem.,287(53):44736-44748 2012
Author:Arai, R., Fukui, S., Kobayashi, N. and Sekiguchi, J.
Abstract:解説は信州大学のプレリリースサイトへ http://www.shinshu-u.ac.jp/faculty/textiles/news/2012/12/50284.html

Domain-Swapped Dimeric Structure of a Stable and Functional De Novo 4-Helix Bundle Protein, WA20
J. Phys. Chem. B,116(23):6789-6797 2012
Author:Arai, R., Kobayashi, N., Kimura, A., Sato, T., Matsuo, K., Wang, A.F., Platt, J.M., Bradley, L.H., Hecht, M.H.
Abstract:解説は信州大学のプレリリースサイトへ http://www.shinshu-u.ac.jp/faculty/textiles/news/2012/03/46854.html

Crystal structure of a menaquinone biosynthetic enzyme, TthMqnD, complexed with its product, 1,4-dihydroxy-6-naphthoate
Photon Factory Activity Report 2010,28:B298 2011
Author:Matsuo, K., Dairi, T., Seto, H. and Arai, R.

Crystal structure of a dimeric de novo 4-helix bundle protein, WA20
Photon Factory Activity Report 2010,28:B256 2011
Author:Arai, R., Kobayashi, N., Kimura, A., Sato, T., Wang, A.F., Platt, J.M., Bradley, L.H. and Hecht, M.H.

Dissecting cell signaling pathways with genetically encoded 3-iodo-L-tyrosine
ChemBioChem,12:387-389 2011
Author:Hayashi A, Hino N, Kobayashi T, Arai R, Shirouzu M, Yokoyama S, Sakamoto K.

Polymorphism screening of brain-expressed FABP7, 5 and 3 genes and association studies in autism and schizophrenia in Japanese subjects
J. Hum. Genet.,55:127-130 2010
Author:Maekawa, M.,Iwayama, Y., Arai, R., Nakamura, K., Ohnishi, T., Toyota, T., Tsujii, M., Okazaki, Y., Osumi, N., Owada, Y., Mori, N., and Yoshikawa, T.

Crystal structure of MqnD (TTHA1568), a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
J. Struct. Biol,168:575-581 2009
Author:Arai, R., Murayama, K., Uchikubo-Kamo, T., Nishimoto, M., Toyama, M., Kuramitsu, S., Terada, T., Shirouzu, M. and Yokoyama, S.

Fabp7 Maps to a Quantitative Trait Locus for a Schizophrenia Endophenotype
PLoS Biol.,5:e297 2007
Author:Watanabe, A., Toyota, T., Owada, Y., Hayashi, T., Iwayama, Y., Matsumata, M., Ishitsuka, Y., Nakaya, A., Maekawa, M., Ohnishi, T., Arai, R., Sakurai, K., Yamada, K., Kondo, H., Hashimoto, K., Osumi, N. and Yoshikawa T.

Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures
Proteins,67:732-742 2007
Author:Arai, R.,* Ito, K.,* Ohnishi, T., Ohba, H., Akasaka, R., Bessho, Y., Hanawa-Suetsugu, K., Yoshikawa, T., Shirouzu, M. and Yokoyama, S.

Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate
Acta Crystallogr. D,63:357-365 2007
Author:Yoshikawa, S.,* Arai, R.,* Kinoshita, Y., Uchikubo-Kamo, T., Wakamatsu, T., Akasaka, R., Masui, R., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S. (*The authors contributed equally to the work.)

Crystal structure of the conserved protein TTHA0727 from Thermus thermophilus HB8 at 1.9 A resolution: A CMD family member distinct from carboxymuconolactone decarboxylase (CMD) and AhpD
Protein Sci.,15:1187-1192 2006
Author:Ito, K.,* Arai, R.,* Fusatomi, E., Kamo-Uchikubo, T., Kawaguchi, S., Akasaka, R., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S.

Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)
Acta Crystallogr. F,62:330-334 2006
Author:Arai, R.,* Yoshikawa, S.,* Murayama, K., Imai, Y., Takahashi, R., Shirouzu, M. and Yokoyama, S.

Crystal structure of the probable haloacid dehalogenase PH0459 from Pyrococcus horikoshii OT3
Protein Sci.,15:373-377 2006
Author:Arai, R., Kukimoto-Niino, M., Kuroishi, C., Bessho, Y., Shirouzu, M. and Yokoyama, S.

Crystal structure of an enhancerof rudimentary homolog (ERH) at 2.1 Angstroms resolution
Protein Sci.,14:1888-1893 2005
Author:Arai, R., Kukimoto-Niino, M., Uda-Tochio, H., Morita, S., Uchikubo-Kamo, T., Akasaka, R., Etou, Y., Hayashizaki, Y., Kigawa, T., Terada, T., Shirouzu, M. and Yokoyama, S.

Conserved protein TTHA1554 from Thermus thermophilus HB8 binds to glutamine synthetase and cystathionine beta-lyase
Biochim. Biophys. Acta,1750:40-47 2005
Author:Arai, R.,* Nishimoto, M.,* Toyama, M., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S. Conserved protein TTHA1554 from Thermus thermophilus HB8 binds to glutamine synthetase and cystathionine beta-lyase

Establishment of stable hFis1 knockdown cells with an siRNA expression vector
J. Biochem.,136:421-425 2004
Author:Arai, R.,* Ito,K.,* Wakiyama, M., Matsumoto, E., Sakamoto, A., Etou, Y., Otsuki, M., Inoue, M., Hayashizaki, Y., Miyagishi, M., Taira, K., Shirouzu, M. and Yokoyama, S.

Phosphorylation analysis of 90 kDa heat shock protein within the cytosolic arylhydrocarbon receptor complex
Biochemistry,43:15510-15519 2004
Author:Ogiso, H., Kagi, N., Matsumoto, E., Nishimoto, M., Arai, R., Shirouzu, M., Mimura, J., Fujii-Kuriyama, Y. and Yokoyama, S.

Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering
Proteins,57:829-838 2004
Author:Arai, R., Wriggers, W., Nishikawa, Y., Nagamune, T. and Fujisawa, T.

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation
J. Immunol. Methods,279:209-218 2003
Author:Ueda, H., Yokozeki, T., Arai, R., Tsumoto, K., Kumagai, I. and Nagamune, T.

A homogeneous noncompetitive immunoassay for the detection of small haptens
Anal. Chem.,74:2500-2504 2002
Author:Yokozeki, T., Ueda, H., Arai, R., Mahoney, W. and Nagamune, T.

A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction
Anal. Chem.,74:5786-5792 2002
Author:Ohiro, Y., Arai, R., Ueda, H. and Nagamune, T.

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein
J. Biosci. Bioeng.,94:362-364 2002
Author:Arai, R., Nakagawa, H., Kitayama, A., Ueda, H. and Nagamune, T

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer
Anal. Biochem.,289:77-81 2001
Author:Arai, R., Nakagawa, H., Tsumoto, K., Mahoney, W., Kumagai, I., Ueda, H. and Nagamune, T.

Design of the linkers which effectively separate domains of a bifunctional fusion protein
Protein Eng.,14:529-532 2001
Author:Arai, R., Ueda, H., Kitayama, A., Kamiya, N. and Nagamune, T.

Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-Test) and improvement of mutagen-sensitivity
J. Biosci. Bioeng,92:301-304 2001
Author:Arai, R., Makita, Y., Oda, Y. and Nagamune, T.

Design of an artificial light-harvesting unit by protein engineering: Cytochrome b(562)-green fluorescent protein chimera
Biochem. Biophys. Res. Commun.,289:299-304 2001
Author:Takeda, S., Kamiya, N., Arai, R. and Nagamune, T.

Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay
Protein Eng.,13:369-376 2000
Author:Arai, R., Ueda, H., Tsumoto, K., Mahoney, W.C., Kumagai, I. and Nagamune, T.

Construction of chimeric proteins between protein G and fluorescence-enhanced green fluorescent protein, and their application to immunoassays
J. Ferment. Bioeng.,86:440-445 1998
Author:Arai, R., Ueda, H. and Nagamune, T.

Presentations
Molecular cloning of novel silk proteins from larvae of caddisfly, Stenopsyche marmorata
8th Chaina International Silk Conference 2013 2013
Presenter:K. Ohkawa, M. Hachisu, T.Nomura, R.Arai, K. Hirabayashi, M. Tsukada and K. Abe

Molecular cloning of novel silk proteins from larvae of caddisfly, Stenopsyche marmorata
Young Researcher Symposium 2013 2013
Presenter:X.Bai, K.Ohkawa, T.Nomura, S.Ishihara, Y.Yamaguchi, M.Tsukada, K.Abe, K.Hirabayashi, R. Arai

Construction and characterization of tandem WA20 domain-swapped de novo proteins with various linkers.
27th Annual Symposium of The Protein Society. 2013
Presenter:Naoya Kobayashi, N., Michael H. Hecht, and Ryoichi Arai

Biochemical natures of underwater silk proteins from caddisfly, Stenopsyche marmorata
The international Textile Conference 2013 2013
Presenter:Kousaku Ohkawa, Masakazu Hachisu, Takaomi Nomura, Ryoichi Arai, Masuhiro Tsukada and Kimio Hirabayashi

Characterization and Nanofiber Fabrication of Underwater Silk Protein from Stenopsyche marmorata
American Chemical Society, National Meething, 2013 Spring 2013
Presenter:Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Kimio Hirabayashi, Masuhiro Tsukada, and Koji Abe

Structural and functional analyses of binary pattern designed de novo proteins
Enzyme Engineering XXII 2013
Presenter:Arai, R. Kobayashi, N., Fukuda, S., Kurasawa, S. and Hecht, M. H.

Solution structure of IseA, an inhibitor protein of DL-endopeptidases from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop
International Conference on Structural Genomics 2013 (ICSG2013) 2013
Presenter:Arai, R., Fukui, S., Kobayashi, N. and Sekiguchi, J.

A De Novo Heme Protein, Dnhps1, Created from a 5-Amino Acid Alphabet
27th Annual Symposium of The Protein Society 2013
Presenter:Fukuda, S., Kurasawa, S., and Arai, R.

Crystal structures of MqnD, a menaquinone biosynthetic enzyme, complexed with the product, 1,4-dihydroxy-6-naphthoate, and its analogs
4th International Symposium on Diffraction Structural Biology 2013 (ISDSB2013) 2013
Presenter:Arai, R., Matsuo, K. and Dairi, T.

Isolation of silk proteins from a caddisfly larva, Stenopsyche marmorata
Textile Bioengneering Informatics Symposium 2012 2012
Presenter:Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Koji Abe, Masuhiro Tsukada and Kimio Hirabayashi

Underwater silk fibers–Biochemical natures for novel textile technology
9th Asia-Pacific Marine Biotechnogy Conference 2012
Presenter:Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Koji Abe, Masuhiro Tsukada and Kimio Hirabayashi

Domain-Swapped Dimeric Structure of a Stable and Functional De Novo Four-Helix Bundle Protein WA20, and its Potential Application
18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012) 2012
Presenter:Arai, R., Kobayashi, N., Kimura, A., Sato, T., and Hecht, M. H.

De novo proteins and interaction analysis using pattern-designed peptide libraries
Gordon Research Conference, Intrinsically Disordered Proteins 2012
Presenter:Arai, R., Kobayashi, N., Kimura, A., Teranishi, H., Sato, T., Bradley, L. H., Nishimura, Y., and Hecht, M. H.

Flight density of aquatic insects fauna over river water surface in the middle reaches of the Shinano River, Japan, with emphasis on Trichoptera.
14th International symposium on Trichoptera 2012
Presenter:Hirabayashi K., Ohkawa K., Arai R., Nomura T., Tsukada M. and Abe K.

Function and Three Dimensional Structure of an Autolysin Inhibitor IseA from Bacillus subtilis
American Society for Microbiology, General Meeting (asm2012) 2012
Presenter:Junichi Sekiguchi and Ryoichi Arai

Repetitive Sequential Motifs of Underwater Silk Protein from Caddisfly, Stenopsyche marmorata,
American Chemical Society, Annual Meeting Spring 2012 2012
Presenter:Kousaku OHKAWA, Yumi MIURA, Takaomi NOMURA, Ryoichi ARAI, Kimio HIRABAYASHI, Masuhiro TSUKADA, and Koji ABE

Domain-swapped dimeric structure of a stable and functional de novo 4-helix bundle protein, WA20
6th International Conference on Advanced Fiber/Textile Materials 2011 2011
Presenter:Kobayashi, N., Kimura, A., Sato, T., Matsuo T., Wang, A. F., Platt, J., Bradley, L. H. Hecht, M. H. and Arai, R.,

Domain-swapped dimeric structure of a de novo 4-helix bundle protein, WA20
PDB40 Symposium (A Special Symposium Celebrating the 40th Anniversary of the Protein Data Bank) 2011
Presenter:Arai, R., Kobayashi, N., Kimura, A., Sato, T., Wang, A. F., Platt, J., Bradley, L. H. and Hecht, M. H.

Crystal structure of a dimeric de novo 4-helix bundle protein WA20
IX European Symposium of The Protein Society 13013, p.87 2011
Presenter:Arai, R., Kimura, A., Kobayashi, N., Sato, T., Wang, A. F., Platt, J., Bradley, L. H. and Hecht, M. H.

Crystal structure of MqnD, a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
Gordon Research Conference, Biopolymers 0 2010
Presenter:Arai, R., Murayama, K., Uchikubo-kamo, T., Nishimoto, M., Toyama, M., Kuramitsu, S., Terada, T., Shirouzu, M. and Yokoyama, S.

Protein Composition in Silk Grand of Caddisfly, Stenopsyche Marmorata, Larva.
14th International Symposium on River and Lake Environments 2009
Presenter:K. Ohkawa, T. Nomura, R.Arai, K. Abe, M. Tsukada, K. Hirabayashi

A four-helix bundle de novo heme protein by phage display screening
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009) 2009
Presenter:R. Arai, M. H. Hecht

Crystal structure of MqnD (TTHA1568), a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology 2009
Presenter:R. Arai, K. Murayama, T. Uchikubo-Kamo, M. Nishimoto, M. Toyama, S. Kuramitsu, T. Terada, M. Shirouzu, S. Yokoyama

Design and construction of functional artificial proteins: Phage display screening of four-helix bundle de novo proteins
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007) 2007
Presenter:R. Arai, M.H. Hecht

Education

Lectures: 2021 , Protein Engineering
2021 , Experiments in Basic Chemistry
2021 , Freshman Seminar
2021 , Biochemistry II
2021 , 情報科学・統計学演習(分担）
2020 , Experiments in Basic Chemistry
2020 , Freshman Seminar
2020 , Biochemistry II
2020 , 情報科学・統計学演習(分担）
2019 , Experiments in Basic Chemistry
2019 , Freshman Seminar
2019 , Biochemistry II
2019 , 情報科学・統計学演習(分担）
2018 , Experiments in Basic Chemistry
2018 , Freshman Seminar
2018 , Biochemistry II
2018 , 情報科学・統計学演習(分担）
2017 , Experiments in Basic Chemistry
2017 , Freshman Seminar
2017 , Biochemistry II
2017 , 情報科学・統計学演習(分担）
2016 , Experiments in Basic Chemistry
2016 , Freshman Seminar
2016 , Biochemistry II
2016 , 情報科学・統計学演習(分担）
2015 , Experiments in Basic Chemistry
2015 , Freshman Seminar
2015 , Biochemistry II
2015 , 情報科学・統計学演習(分担）
2014 , Experiments in Basic Chemistry
2014 , Freshman Seminar
2014 , Biochemistry II
2014 , 情報科学・統計学演習(分担）
2013 , Seminar in Scientific Presentation Writing and Presentation
2013 , Practical Exercises of Field Science
2013 , Freshman Seminar
2013 , Experiments in Basic Chemistry
2013 , Biochemistry II
2013 , Experiments in Bioresource Application
2013 , 情報科学・統計学演習(分担）
2012 , Seminar in Scientific Presentation Writing and Presentation
2012 , Practical Exercises of Field Science
2012 , Freshman Seminar
2012 , Biochemistry II
2012 , Experiments in Bioresource Application
2011 , Seminar in Scientific Presentation Writing and Presentation
2011 , Practical Exercises of Field Science
2011 , Biochemistry II
2011 , Experiments in Bioresource Application
2010 , Seminar in Scientific Presentation Writing and Presentation
2010 , Practical Exercises of Field Science
2010 , Biochemistry II
2010 , Experiments in Bioresource Application
2009 , Experiments in Molecular Bioengineering
2009 , Biochemistry II
2008 , Molecular Biology II
2008 , Experiments in Molecular Bioengineering

Thesis Guidance: 2021
Graduation Thesis Number of Students Total：3
Master's Thesis Number of Students Total：2
Doctor's Thesis Number of Students Total：1

2020
Graduation Thesis Number of Students Total：3
Master's Thesis Number of Students Total：2
Doctor's Thesis Number of Students Total：1

2019
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：3
Doctor's Thesis Number of Students Total：1

2018
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：5

2017
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：3

2016
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：2
Doctor's Thesis Number of Students Total：1

2015
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：4
Doctor's Thesis Number of Students Total：1

2014
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：4
Doctor's Thesis Number of Students Total：1

2013
Graduation Thesis Number of Students Total：3
Master's Thesis Number of Students Total：2(International Students:1)
Doctor's Thesis Number of Students Total：1

2012
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：4(International Students:1)
Researcher's Thesis Number of Students Total：1

2011
Graduation Thesis Number of Students Total：2(International Students:1)
Master's Thesis Number of Students Total：4

2010
Graduation Thesis Number of Students Total：2
Master's Thesis Number of Students Total：2

2009
Graduation Thesis Number of Students Total：2

2008
Graduation Thesis Number of Students Total：1

International Student Receipt: 2013 , First Term:1 , Second Term:1 , M2
2012 , First Term:1 , Second Term:1 , M1
2011 , First Term:1 , Second Term:1 , B4

Shinshu University Researcher Directory

ARAI Ryoichi

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ARAI　Ryoichi