Ph.D., University of Tokyo

Protein engineering, Protein design, Structural biology, Biotechnology, Synthetic biology

2019
Department of Biomolecular Innovation, Institute for Biomedical Sciences, ICCER, Shinshu University

2017 - 2021
Journal of Bioscience and Bioengineering, Editor

2016 - 2019
Department Head, Department of Supramolecular Complexes, Research Center for Fungal and Microbial Dynamism, Shinshu University

2016
Assistant Professor, School of Science and Technology, Academic Assembly, Shinshu University

2014 - 2016
Assistant Professor, School of Science and Technology, Academic Assembly, Shinshu University

2013 - 2018
Visiting Researcher, Division of Structural and Synthetic Biology, Center for Life Science and Technologies, RIKEN

2012 - 2014
Assistant Professor, Faculty of Textile Science and Technology, Shinshu University

2007 - 2012
Assistant Professor, International Young Researchers Empowerment Center, Shinshu University

2006 - 2007
JSPS Postdoctoral Fellow for Research Abroad (Prof. Michael H. Hecht Lab, Princeton University, NJ, USA)

2004 - 2006
Research Scientist, Genomic Sciences Center, RIKEN Yokohama Institute (Dr. Shigeyuki Yokoyama Gr.)

2001 - 2004
Research Associate, Genomic Sciences Center, RIKEN Yokohama Institute (Dr. Shigeyuki Yokoyama Gr.)

1998 - 2001
JSPS Research Fellow (DC1)

2019 - 2019, Visiting Scholar, Institute for Protein Design, University of Washington, WA, USA (Prof. David Baker Lab.)

2006 - 2007, Research fellow, Department of Chemistry, Princeton University, NJ, USA (Prof. Michael H. Hecht Lab.)

2001, The University of Tokyo, Graduate School of Engineering, Department of Chemistry and Biotechnology

1996, The University of Tokyo, School of Engineering, Department of Chemistry and Biotechnology

1998, The University of Tokyo, Graduate School of Engineering, Department of Chemistry and Biotechnology

2015
Japanese society of enzyme engineering, Encouraging prize of Japanese society of enzyme engineering
"Study on structural analysis of a de novo protein and its application to construction of nanostructures"

2013
Merck Millipore, Leave a Nest Grant Direct Detect Award

2011
Protein Data Bank 40th Anniversary Symposium, PDB40 Travel Award
Protein Data Bank 40th Anniversary Symposium

2011
The Protein Society, Protein Science Young Investigator Travel Grant / The Protein Society Finn Wold Travel Award
IX European Symposium of The Protein Society

2001
財団法人総合研究奨励会, Ishii Science Promotion Award, General Research Promotion Foundation

1996
化学工学会, Award for graduate student, The Society of Chemical Engineers, Japan

Crystal structure and activity of a de novo enzyme, ferric enterobactin esterase Syn-F4
Kodai Kurihara, Koji Umezawa, Ann E. Donnelly, Brendan Sperling, Guanyu Liao, Michael H. Hecht, and Ryoichi Arai
Proc. Natl. Acad. Sci. U.S.A., 120(38), e2218281120, Sep. 2023, Refereed
Last, Corresponding

Enzymatic Fabrication of Multifunctional Amyloid-based Protein Assembly
Mitsuki Nakamura, Ryoichi Arai, Noriho Kamiya, Masafumi Sakono
Chemistry Letters, 52(7), 538-541, Jul. 2023, Refereed

One-pot synthesis of fibrillar-shaped functional nanomaterial using microbial transglutaminase
Sakono M, Nakamura M, Ohshima T, Miyakoshi A, Arai R, Minamihata K, Kamiya N.
J. Biosci. Bioeng., 135(6), 440-446, Jun. 2023, Refereed

Reversible Assembly of an Artificial Protein Nanocage Using Alkaline Earth Metal Ions
Ohara, Naoya; Kawakami, Norifumi; Arai, Ryoichi; Adachi, Naruhiko; Moriya, Toshio; Kawasaki, Masato; Miyamoto, Kenji;
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 145(1), 216-223, Jan. 2023, Refereed

Multi-enzyme Machinery for Chitin Degradation in the Chitinolytic Bacterium Chitiniphilus shinanonensis SAY3T
Hiroaki Sato, Norie Sonoda, Moe Nakano, Yuka Matsuyama, Arisa Shizume, Ryoichi Arai, Masahiro Nogawa, Goro Taguchi & Makoto Shimosaka
CURRENT MICROBIOLOGY, 80, 360, 2023, Refereed

Production of pentaglycine-fused proteins using Escherichia coli expression system without in vitro peptidase treatment
Sakono, Masafumi; Oshima, Tatsuki; Iwakawa, Takako; Arai, Ryoichi;
PROTEIN EXPRESSION AND PURIFICATION, 194, 106068, Jun. 2022, Refereed

Self-Assembling Lectin Nano-Block Oligomers Enhance Binding Avidity to Glycans
Shin Irumagawa, Keiko Hiemori, Sayoko Saito, Hiroaki Tateno, Ryoichi Arai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(2), 676, Jan. 2022, Refereed
Last, Corresponding

Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60
Junya Obata, Norifumi Kawakami, Akihisa Tsutsumi, Erika Nasu, Kenji Miyamoto, Masahide Kikkawa, Ryoichi Arai
Chemical Communications, 57(79), 10226-10229, Sep. 2021, Refereed
Last, Corresponding

Rational thermostabilisation of four-helix bundle dimeric de novo proteins
Shin Irumagawa, Kaito Kobayashi, Yutaka Saito, Takeshi Miyata, Mitsuo Umetsu, Tomoshi Kameda & Ryoichi Arai
SCIENTIFIC REPORTS, 11, 7526, 06 Apr. 2021, Refereed

Hyperstable De Novo Protein with a Dimeric Bisecting Topology
Kimura, N., Mochizuki, K., Umezawa K., Hecht, M.H., Arai, R.
ACS Synthetic Biology, 9(2), 254-259, 2020, Refereed

Lectin engineering: the possible and the actual
Hirabayashi, J., Arai, R.
Interface Focus, 9, 20180068, 2019, Refereed

Comparative analysis of plant isochorismate synthases reveals structural mechanisms underlying their distinct biochemical properties
2. Yokoo, S., Inoue, S., Suzuki, N., Amakawa, N., Matsui, H., Nakagami, H., Takahashi, A., Arai R. and Katou, S.
BIOSCIENCE REPORTS, 38, BSR20171457, 2018, Refereed

Hierarchical design of artificial proteins and complexes toward synthetic structural biology
Arai, R.
Biophysical Reviews, 10, 391-410, 2018, Refereed

Design of hollow protein nanoparticles with modifiable interior and exterior surfaces
Norifumi Kawakami, Hiroki Kondo, Yuki Matsuzawa, Kaoru Hayasaka, Erika Nasu, Kenji Sasahara, Ryoichi Arai, Kenji Miyamoto
Angewandte Chemie International Edition, 57, 12400-12404, 2018, Refereed

Self-assembling supramolecular nanostructures constructed from de novo extender protein nanobuilding blocks
Naoya Kobayashi, Kouichi Inano, Kenji Sasahara, Takaaki Sato, Keisuke Miyazawa, Takeshi Fukuma, Michael H Hecht, Chihong Song, Kazuyoshi Murata, and Ryoichi Arai
ACS Synthetic Biology, 7(5), 1381-1394, 2018, Refereed

Design and construction of self-assembling supramolecular protein complexes using artificial and fusion proteins as nanoscale building blocks
Kobayashi, N. and Arai, R.
Current Opinion in Biotechnology, 46, 57–65, 2017, Refereed

Characterization of silk gland ribosomes from a bivoltine caddisfly, Stenopsyche marmorata: translational suppression of a silk protein in cold conditions.
Takaomi Nomura, Miho Ito, Mai Kanamori, Yuta　Shigeno,　Toshio Uchiumi, Ryoichi Arai, Masuhiro Tsukada, Kimio Hirabayashi, Kousaku Ohkawa
Biochemical and biophysical research communications, 469, 210-215, 2016, Refereed

Flight density of the aquatic insect fauna over the water surface in the middle reaches of the Shinano River, Japan, mainly among caddisflies (Trichoptera)
Hirabayashi, K.*, Ikutama, E., Ohkawa, K., Arai, R., Nomura, T., Tsukada, M., and Abe, K.
Zoosymposia, 10, 203–213, 2016

Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis
Miruku Ueda, Makoto Shimosaka and Ryoichi Arai
Acta Cryst. F, F71(12), 1516–1520, Dec. 2015, Refereed

Crystal structure of glycine oxidase from Geobacillus kaustophilus
Shiono, T., Nomura, T., Nishiya, Y., and Arai, R.
Photon Factory Activity Report 2014, 32(B), 204, 2015

Crystal structure of Fab fragment of an anti-Osteocalcin C-Terminal peptide antibody KTM219
Komatsu, M., Dong, J., Ueda, H., and Arai, R.
Photon Factory Activity Report 2014, 32(B), 205, 2015

Crystal structure of ChiL, a chitinase from Chitiniphilus shinanonensis
Ueda, M., Shimosaka, M., and Arai, R.
Photon Factory Activity Report 2014, 32(B), 206, 2015

Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
Xue Bai, Mayo Sakaguchi, Yuko Yamaguchi, Shiori Ishihara, Masuhiro Tsukada, Kimio Hirabayashi, Kousaku Ohkawa, Takaomi Nomura, Ryoichi Arai
Biochem. Biophys. Res. Commun., 464(3), 814–819, 2015, Refereed

Self-Assembling Nano-Architectures Created from a Protein Nano-Building Block Using an Intermolecularly Folded Dimeric de Novo Protein
Naoya Kobayashi, Keiichi Yanase, Takaaki Sato, Satoru Unzai, Michael H. Hecht, Ryoichi Arai
J. Am. Chem. Soc., 137(35), 11285–11293, 2015, Refereed

Intermolecular interactions and molecular dynamics in bovine serum albumin solutions studied by small angle X-ray scattering and dielectric relaxation spectroscopy.
Yanase, K., Arai R., and Sato, T.
J. Mol. Liq., 200(A), 59-66, 2014, Refereed

Functional characterization of FABP3, 5 and 7 gene variants identified in schizophrenia and autism spectrum disorder and mouse behavioral studies.
Shimamoto C, Ohnishi T, Maekawa M, Watanabe A, Ohba H, Arai R, Iwayama Y,　Hisano Y, Toyota T, Toyoshima M, Suzuki K, Shirayama Y, Nakamura K, Mori N, Owada, Y, Kobayashi T, and Yoshikawa T
Hum. Mol. Genet., 23, 6495-6511, 2014, Refereed

Crystal structure of glycine oxidase from Bacillus thuringiensis
Ohishi, S., Shiono, T., Nishiya, Y., Nomura, T., and Arai, R.
Photon Factory Activity Report 2013, 31(B), 274, 2014

Chain conformational study on underwater silk protein from caddisfly, Stenopsyche marmorata: Implication of a fiber-forming mechanism
Ohkawa, K., Hachisu, M., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe, K
Adv. Mater. Res., 796, 41706, 2013

バイナリーパターンデザインによるデノボタンパク質WA20のドメインスワップ二量体構造
小林直也，*新井亮一
酵素工学研究会誌, 69, 19-25, 2013

Long-range periodic sequence of Stenopsyche marmorata cement/silk protein: Purification and biochemical characterization
Ohkawa, K., Miura, Y., Nomura, T., Arai, R., Abe, K., Tsukada M. and Hirabayashi, K.
Biofouling, 29(4), 357-367, 2013, Refereed

Isolation of silk proteins from a caddisfly larva, Stenopsyche marmorata.
Ohkawa, K., Miura, Y., Nomura, T., Arai, R., Abe, K., Tsukada M. and Hirabayashi, K.
J. Fiber Bioeng. Informatics, 5, 125-137, 2012

Domain-Swapped Dimeric Structure of a Stable and Functional De Novo 4-Helix Bundle Protein, WA20
Arai, R., Kobayashi, N., Kimura, A., Sato, T., Matsuo, K., Wang, A.F., Platt, J.M., Bradley, L.H., Hecht, M.H.
J. Phys. Chem. B, 116(23), 6789-6797, 2012, Refereed

Solution structure of IseA, an inhibitor protein of DL-endopeptidases from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop
Arai, R., Fukui, S., Kobayashi, N. and Sekiguchi, J.
J. Biol. Chem., 287(53), 44736-44748, 2012, Refereed

Crystal structure of a dimeric de novo 4-helix bundle protein, WA20
Arai, R., Kobayashi, N., Kimura, A., Sato, T., Wang, A.F., Platt, J.M., Bradley, L.H. and Hecht, M.H.
Photon Factory Activity Report 2010, 28, B256, 2011

Crystal structure of a menaquinone biosynthetic enzyme, TthMqnD, complexed with its product, 1,4-dihydroxy-6-naphthoate
Matsuo, K., Dairi, T., Seto, H. and Arai, R.
Photon Factory Activity Report 2010, 28, B298, 2011

Dissecting cell signaling pathways with genetically encoded 3-iodo-L-tyrosine
Hayashi A, Hino N, Kobayashi T, Arai R, Shirouzu M, Yokoyama S, Sakamoto K.
ChemBioChem, 12, 387-389, 2011, Refereed

Polymorphism screening of brain-expressed FABP7, 5 and 3 genes and association studies in autism and schizophrenia in Japanese subjects
Maekawa, M.,Iwayama, Y., Arai, R., Nakamura, K., Ohnishi, T., Toyota, T., Tsujii, M., Okazaki, Y., Osumi, N., Owada, Y., Mori, N., and Yoshikawa, T.
J. Hum. Genet., 55, 127-130, 2010, Refereed

Crystal structure of MqnD (TTHA1568), a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
Arai, R., Murayama, K., Uchikubo-Kamo, T., Nishimoto, M., Toyama, M., Kuramitsu, S., Terada, T., Shirouzu, M. and Yokoyama, S.
J. Struct. Biol, 168, 575-581, 2009, Refereed

Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate
Yoshikawa, S.,* Arai, R.,* Kinoshita, Y., Uchikubo-Kamo, T., Wakamatsu, T., Akasaka, R., Masui, R., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S. (*The authors contributed equally to the work.)
Acta Crystallogr. D, 63, 357-365, 2007, Refereed

Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures
Arai, R.,* Ito, K.,* Ohnishi, T., Ohba, H., Akasaka, R., Bessho, Y., Hanawa-Suetsugu, K., Yoshikawa, T., Shirouzu, M. and Yokoyama, S.
Proteins, 67, 732-742, 2007, Refereed

Fabp7 Maps to a Quantitative Trait Locus for a Schizophrenia Endophenotype
Watanabe, A., Toyota, T., Owada, Y., Hayashi, T., Iwayama, Y., Matsumata, M., Ishitsuka, Y., Nakaya, A., Maekawa, M., Ohnishi, T., Arai, R., Sakurai, K., Yamada, K., Kondo, H., Hashimoto, K., Osumi, N. and Yoshikawa T.
PLoS Biol., 5, E297, 2007, Refereed

Crystal structure of the probable haloacid dehalogenase PH0459 from Pyrococcus horikoshii OT3
Arai, R., Kukimoto-Niino, M., Kuroishi, C., Bessho, Y., Shirouzu, M. and Yokoyama, S.
Protein Sci., 15, 373-377, 2006, Refereed

Structure of human ubiquitin-conjugating enzyme E2 G2 (UBE2G2/UBC7)
Arai, R.,* Yoshikawa, S.,* Murayama, K., Imai, Y., Takahashi, R., Shirouzu, M. and Yokoyama, S.
Acta Crystallogr. F, 62, 330-334, 2006, Refereed

Crystal structure of the conserved protein TTHA0727 from Thermus thermophilus HB8 at 1.9 A resolution: A CMD family member distinct from carboxymuconolactone decarboxylase (CMD) and AhpD
Ito, K.,* Arai, R.,* Fusatomi, E., Kamo-Uchikubo, T., Kawaguchi, S., Akasaka, R., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S.
Protein Sci., 15, 1187-1192, 2006, Refereed

Conserved protein TTHA1554 from Thermus thermophilus HB8 binds to glutamine synthetase and cystathionine beta-lyase
Arai, R.,* Nishimoto, M.,* Toyama, M., Terada, T., Kuramitsu, S., Shirouzu, M. and Yokoyama, S. Conserved protein TTHA1554 from Thermus thermophilus HB8 binds to glutamine synthetase and cystathionine beta-lyase
Biochim. Biophys. Acta, 1750, 40-47, 2005, Refereed

Crystal structure of an enhancerof rudimentary homolog (ERH) at 2.1 Angstroms resolution
Arai, R., Kukimoto-Niino, M., Uda-Tochio, H., Morita, S., Uchikubo-Kamo, T., Akasaka, R., Etou, Y., Hayashizaki, Y., Kigawa, T., Terada, T., Shirouzu, M. and Yokoyama, S.
Protein Sci., 14, 1888-1893, 2005, Refereed

Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering
Arai, R., Wriggers, W., Nishikawa, Y., Nagamune, T. and Fujisawa, T.
Proteins, 57, 829-838, 2004, Refereed

Phosphorylation analysis of 90 kDa heat shock protein within the cytosolic arylhydrocarbon receptor complex
Ogiso, H., Kagi, N., Matsumoto, E., Nishimoto, M., Arai, R., Shirouzu, M., Mimura, J., Fujii-Kuriyama, Y. and Yokoyama, S.
Biochemistry, 43, 15510-15519, 2004, Refereed

Establishment of stable hFis1 knockdown cells with an siRNA expression vector
Arai, R.,* Ito,K.,* Wakiyama, M., Matsumoto, E., Sakamoto, A., Etou, Y., Otsuki, M., Inoue, M., Hayashizaki, Y., Miyagishi, M., Taira, K., Shirouzu, M. and Yokoyama, S.
J. Biochem., 136, 421-425, 2004, Refereed

An optimized homogeneous noncompetitive immunoassay based on the antigen-driven enzymatic complementation
Ueda, H., Yokozeki, T., Arai, R., Tsumoto, K., Kumagai, I. and Nagamune, T.
J. Immunol. Methods, 279, 209-218, 2003, Refereed

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein
Arai, R., Nakagawa, H., Kitayama, A., Ueda, H. and Nagamune, T
J. Biosci. Bioeng., 94, 362-364, 2002, Refereed

A homogeneous and noncompetitive immunoassay based on the enhanced fluorescence resonance energy transfer by leucine zipper interaction
Ohiro, Y., Arai, R., Ueda, H. and Nagamune, T.
Anal. Chem., 74, 5786-5792, 2002, Refereed

A homogeneous noncompetitive immunoassay for the detection of small haptens
Yokozeki, T., Ueda, H., Arai, R., Mahoney, W. and Nagamune, T.
Anal. Chem., 74, 2500-2504, 2002, Refereed

Design of an artificial light-harvesting unit by protein engineering: Cytochrome b(562)-green fluorescent protein chimera
Takeda, S., Kamiya, N., Arai, R. and Nagamune, T.
Biochem. Biophys. Res. Commun., 289, 299-304, 2001, Refereed

Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-Test) and improvement of mutagen-sensitivity
Arai, R., Makita, Y., Oda, Y. and Nagamune, T.
J. Biosci. Bioeng, 92, 301-304, 2001, Refereed

Design of the linkers which effectively separate domains of a bifunctional fusion protein
Arai, R., Ueda, H., Kitayama, A., Kamiya, N. and Nagamune, T.
Protein Eng., 14, 529-532, 2001, Refereed

Demonstration of a homogeneous noncompetitive immunoassay based on bioluminescence resonance energy transfer
Arai, R., Nakagawa, H., Tsumoto, K., Mahoney, W., Kumagai, I., Ueda, H. and Nagamune, T.
Anal. Biochem., 289, 77-81, 2001, Refereed

Fluorolabeling of antibody variable domains with green fluorescent protein variants: application to an energy transfer-based homogeneous immunoassay
Arai, R., Ueda, H., Tsumoto, K., Mahoney, W.C., Kumagai, I. and Nagamune, T.
Protein Eng., 13, 369-376, 2000, Refereed

Construction of chimeric proteins between protein G and fluorescence-enhanced green fluorescent protein, and their application to immunoassays
Arai, R., Ueda, H. and Nagamune, T.
J. Ferment. Bioeng., 86, 440-445, 1998, Refereed

Protein Cages, Methods in Molecular Biology 2671
Naoya Kobayashi and Ryoichi Arai, Contributor, Protein Cages and Nanostructures Constructed from Protein Nanobuilding Blocks
Humana Press (Springer Nature), 79-94 Jun. 2023

Methods in Enzymology 647
Ryoichi Arai, Contributor, Linkers in Biomacromolecules Chapter 8 "Design of helical linkers for fusion proteins and protein-based nanostructures"
Elsevier, 209-230 2021, Refereed
ISBN:10.1016/bs.mie.2020.10.003

Biotechnology of Silk: Biologically inspired systems Volume 5
Ohkawa, K., Nomura, T., Arai, R., Hirabayashi, K., Tsukada, M., and Abe , K., Contributor, Characterization of underwater silk proteins from caddisfly larva, Stenopsyche marmorata
Springer, New York, Ch. 6, pp. 107-122 2013

Crystal structures of MqnD, a menaquinone biosynthetic enzyme, complexed with the product, 1,4-dihydroxy-6-naphthoate, and its analogs
Arai, R., Matsuo, K. and Dairi, T.
4th International Symposium on Diffraction Structural Biology 2013 (ISDSB2013), 2013

A De Novo Heme Protein, Dnhps1, Created from a 5-Amino Acid Alphabet
Fukuda, S., Kurasawa, S., and Arai, R.
27th Annual Symposium of The Protein Society, 2013

Solution structure of IseA, an inhibitor protein of DL-endopeptidases from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop
Arai, R., Fukui, S., Kobayashi, N. and Sekiguchi, J.
International Conference on Structural Genomics 2013 (ICSG2013), 2013

Structural and functional analyses of binary pattern designed de novo proteins
Arai, R. Kobayashi, N., Fukuda, S., Kurasawa, S. and Hecht, M. H.
Enzyme Engineering XXII, 2013

Characterization and nanofiber fabrication of underwater silk protein from Stenopsyche marmorata
Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Kimio Hirabayashi, Masuhiro Tsukada, and Koji Abe
American Chemical Society, National Meething, 2013 Spring, 2013

Biochemical natures of underwater silk proteins from caddisfly, Stenopsyche marmorata
Kousaku Ohkawa, Masakazu Hachisu, Takaomi Nomura, Ryoichi Arai, Masuhiro Tsukada and Kimio Hirabayashi
The International Textile Conference 2013, 2013

Construction and characterization of tandem WA20 domain-swapped de novo proteins with various linkers.
Naoya Kobayashi, N., Michael H. Hecht, and Ryoichi Arai
27th Annual Symposium of The Protein Society., 2013

Molecular cloning of novel silk proteins from larvae of caddisfly, Stenopsyche marmorata
X.Bai, K.Ohkawa, T.Nomura, S.Ishihara, Y.Yamaguchi, M.Tsukada, K.Abe, K.Hirabayashi, R. Arai
Young Researcher Symposium 2013, 2013

Molecular cloning of novel silk proteins from larvae of caddisfly, Stenopsyche marmorata
K. Ohkawa, M. Hachisu, T.Nomura, R.Arai, K. Hirabayashi, M. Tsukada and K. Abe
8th Chaina International Silk Conference 2013, 2013

De novo proteins and interaction analysis using pattern-designed peptide libraries
Arai, R., Kobayashi, N., Kimura, A., Teranishi, H., Sato, T., Bradley, L. H., Nishimura, Y., and Hecht, M. H.
Gordon Research Conference, Intrinsically Disordered Proteins, 2012

Domain-Swapped Dimeric Structure of a Stable and Functional De Novo Four-Helix Bundle Protein WA20, and its Potential Application
Arai, R., Kobayashi, N., Kimura, A., Sato, T., and Hecht, M. H.
18th Symposium of Young Asian Biochemical Engineers' Community (YABEC2012), 2012

Underwater silk fibers–Biochemical natures for novel textile technology
Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Koji Abe, Masuhiro Tsukada and Kimio Hirabayashi
9th Asia-Pacific Marine Biotechnogy Conference, 2012

Isolation of silk proteins from a caddisfly larva, Stenopsyche marmorata
Kousaku Ohkawa, Yumi Miura, Takaomi Nomura, Ryoichi Arai, Koji Abe, Masuhiro Tsukada and Kimio Hirabayashi
Textile Bioengneering Informatics Symposium 2012, 2012

Repetitive Sequential Motifs of Underwater Silk Protein from Caddisfly, Stenopsyche marmorata, 
Kousaku OHKAWA, Yumi MIURA, Takaomi NOMURA, Ryoichi ARAI, Kimio HIRABAYASHI, Masuhiro TSUKADA, and Koji ABE
American Chemical Society, Annual Meeting Spring 2012, 2012

Function and Three Dimensional Structure of an Autolysin Inhibitor IseA from Bacillus subtilis
Junichi Sekiguchi and Ryoichi Arai
American Society for Microbiology, General Meeting (asm2012), 2012

Flight density of aquatic insects fauna over river water surface in the middle reaches of the Shinano River, Japan, with emphasis on Trichoptera.
Hirabayashi K., Ohkawa K., Arai R., Nomura T., Tsukada M. and Abe K.
14th International symposium on Trichoptera, 2012

Crystal structure of a dimeric de novo 4-helix bundle protein WA20
Arai, R., Kimura, A., Kobayashi, N., Sato, T., Wang, A. F., Platt, J., Bradley, L. H. and Hecht, M. H.
IX European Symposium of The Protein Society 13013, p.87, 2011

Domain-swapped dimeric structure of a de novo 4-helix bundle protein, WA20
Arai, R., Kobayashi, N., Kimura, A., Sato, T., Wang, A. F., Platt, J., Bradley, L. H. and Hecht, M. H.
PDB40 Symposium (A Special Symposium Celebrating the 40th Anniversary of the Protein Data Bank), 2011

Domain-swapped dimeric structure of a stable and functional de novo 4-helix bundle protein, WA20
Kobayashi, N., Kimura, A., Sato, T., Matsuo T., Wang, A. F., Platt, J., Bradley, L. H. Hecht, M. H. and Arai, R.,
6th International Conference on Advanced Fiber/Textile Materials 2011, 2011

Crystal structure of MqnD, a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
Arai, R., Murayama, K., Uchikubo-kamo, T., Nishimoto, M., Toyama, M., Kuramitsu, S., Terada, T., Shirouzu, M. and Yokoyama, S.
Gordon Research Conference, Biopolymers 0, 2010

Crystal structure of MqnD (TTHA1568), a menaquinone biosynthetic enzyme from Thermus thermophilus HB8
R. Arai, K. Murayama, T. Uchikubo-Kamo, M. Nishimoto, M. Toyama, S. Kuramitsu, T. Terada, M. Shirouzu, S. Yokoyama
21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology, 2009

A four-helix bundle de novo heme protein by phage display screening
R. Arai, M. H. Hecht
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009), 2009

Protein Composition in Silk Grand of Caddisfly, Stenopsyche Marmorata, Larva.
K. Ohkawa, T. Nomura, R.Arai, K. Abe, M. Tsukada, K. Hirabayashi
14th International Symposium on River and Lake Environments, 2009

Design and construction of functional artificial proteins: Phage display screening of four-helix bundle de novo proteins
R. Arai, M.H. Hecht
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007), 2007

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

情報科学・統計学演習(分担）
Shinshu University

Molecular Biology II
Shinshu University

Experiments in Molecular Bioengineering
Shinshu University

Experiments in Molecular Bioengineering
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Seminar in Scientific Presentation Writing and Presentation
Shinshu University

Seminar in Scientific Presentation Writing and Presentation
Shinshu University

Practical Exercises of Field Science
Shinshu University

Experiments in Bioresource Application
Shinshu University

Practical Exercises of Field Science
Shinshu University

Experiments in Bioresource Application
Shinshu University

Biochemistry II
Shinshu University

Seminar in Scientific Presentation Writing and Presentation
Shinshu University

Practical Exercises of Field Science
Shinshu University

Experiments in Bioresource Application
Shinshu University

Biochemistry II
Shinshu University

Seminar in Scientific Presentation Writing and Presentation
Shinshu University

Practical Exercises of Field Science
Shinshu University

Experiments in Bioresource Application
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Experiments in Basic Chemistry
Shinshu University

Freshman Seminar
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Biochemistry II
Shinshu University

Protein Engineering
Shinshu University

Biochemistry II
Shinshu University

Protein Science Society of Japan

The Society for Biotechnology, Japan

The Molecular Biology Society of Japan

Japanese Society of Enzyme Engineering

Division of Biofunctional Chemistry, the Chemical Society of Japan

Japanese Society for Cell Synthesis Research

Design and construction of protein nanobuilding block complexes

Design and development of artificial proteins

Construction and application of engineered proteins and fusion/chimeric proteins

Protein X-ray crystallography

2009, 2, 2

2008, 1, 1

2017, 2, 2, 3

2018, 2, 2, 5, 3

2010, 2, 2, 2, 1

2011, 2, 1, 2, 4, 1

2012, 2, 2, 4, 1, 3, 1

2020, 3, 3, 2, 1, 1

2019, 2, 2, 3, 2, 1

2021, 3, 2, 1

2013, 3, 3, 2, 1, 1, 1, 1

2014, 2, 2, 4, 1, 1

2015, 2, 2, 4, 3, 1

2016, 2, 2, 2, 1, 1, 1

2011, 1, 1, B4

2013, 1, 1, M2

2012, 1, 1, M1