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奥村伸生  オクムラ ノブオ

教員組織学術研究院(保健学系)電話番号0263-37-2392
教育組織医学部 保健学科 病因・病態検査学FAX番号0263-37-2370
職名教授メールアドレスnobuoku@shinshu-u.ac.jp
住所〒390-8624 松本市旭3-1-1ホームページURL

更新日:2019/09/10

プロフィール

研究分野
病態検査学
病態医化学
現在の研究課題
シトルリン化フィブリノゲンとフィブリンの機能解析
キーワード:シトルリン化フィブリノゲン , シトルリン化フィブリン , 2007-
フィブリノゲン欠損症発症機序の分子生物学的解析
キーワード:フィブリノゲン欠損症 , 1997-
異常フィブリノゲンの機能異常の解析
キーワード:異常フィブリノゲン , 1993-
所属学会
所属学会
日本臨床検査医学会
臨床化学会
日本血栓止血学会
日本検査血液学会
日本臨床検査自動化学会
International Fibrinogen Research Society
International Society for Thrombosis and Haemostasis
学歴
出身学校・専攻等(大学院を除く)
1982 , 東京理科大学 , 理学部
1977 , 信州大学医療技術短期大学部

取得学位
博士(医学) , 信州大学

免許・資格等
1977 , 臨床検査技師
受賞学術賞
2006 , 緒方富雄賞
2004 , Bergmeyer-Kawai賞
1995 , 小島三郎記念技術賞

研究活動業績

研究業績(著書・
発表論文等)
著書
電気泳動法 他
医歯薬出版(東京) , 臨床化学検査学 , :64-70 他 2016(Mar. 25)
Author:奥村伸生


穿刺液検査 他
金原出版 , 臨床検査法提要 改訂第34版  , :199-211 他 2015
Author:奥村伸生


プラスミッドDNAの調製.他
医歯薬出版(東京) , 遺伝子検査学実習書 , :114-119,他 2010
Author:奥村伸生


第6章 臨床化学検査, ⅠB6.主要血漿蛋白成分の測定.他
金原出版(東京) , 臨床検査法提要 改訂第33版 , :413-416,他 2010
Author:奥村伸生


タンパク質.他
医歯薬出版(東京) , 臨床化学検査学第3版 , :155-175,他 2010
Author:奥村伸生


電気泳動法、ほか
臨床化学検査学第2版 , :104-110ほか 2006
Author:奥村伸生


穿刺液・髄液検査ほか
金原出版 , 臨床検査法提要32版 , :237-247ほか 2005
Author:奥村伸生


電気泳動法、ほか
医歯薬出版 , 臨床化学検査学 , :103-107ほか 2003
Author:奥村伸生


各種臨床検査の基準範囲一覧
金原出版 , 臨床検査法提要31版 , :3-23頁 1998
Author:奥村伸生


臨床検査用略号
金原出版 , 臨床検査法提要31版 , :27-47頁 1998
Author:奥村伸生


論文
γD318Y fibrinogen shows no fibrin polymerization due to defective “A-a” and “B-b” interactions, whereas that of γK321E fibrinogen is nearly normal
Thromb Res,182:150-158 2019
Author:KamijoT, Mukai S, Taira C, Higuchi Y, Okumura N.


フィブリノゲン活性の偽低値と異常凝固波形を示したIgA M 蛋白血症の解析
臨床病理,67:449-456 2019
Author:新井慎平,上條途夢,竹澤由夏,菅野光俊,上原剛,本田孝行,奥村伸生.


FGA 1238bp欠失を有する無フィブリノゲン血症3例のハプロタイプ解析
医学検査,68:589-595 2019
Author:平 千明, 竹澤由夏, 新井慎平, 松田和之, 上條途夢, 樋口由美子, 奥村伸生


ファクターオートフィブリノーゲンの基礎的性能評価とフィブリノーゲン異常症例における測定値の検討
医学検査,67:7-12 2018
Author:永田和宏,新井慎平,向井早紀,竹澤由夏,菅野光俊,本田孝行,奥村伸生


Fibrin monomers derived from thrombogenic dysfibrinogenemia, Naples-type variant (BβAla68Thr), showed almost entirely normal polymerization
Thromb Res,172:1-3 2018
Author:Kamijo T, Nagata K, Taira C, Higuchi Y, Arai S, Okumura N


フィブリノゲン蓄積病を呈する異常フィブリノゲン産生細胞の線維状封入体形成と小胞体ストレス応答
臨床病理,66:1058-1064 2018
Author:上條途夢,大久保奈緒,平千明,樋口由美子,新井慎平,奥村伸生


DVTを発症した先天性フィブリノゲン異常症患者Fibrinogen Matsumoto I のFDP-E分画異常高値の精査
検査血液,19:306-313 2018
Author:新井慎平,中越りつこ,倉田淳一,川崎健治,菅野光俊,上原剛,本田孝行,奥村伸生


Hereditary Fibrinogen Aα-chain amyloidosis in Asia: clinical andmolecular characteristics.
Int. J. Mol. Sci.,19:320; doi:10.3390/ijms19010320 2018
Author:Yazaki M, Yoshinaga T, Sekijima Y, Kametani F, Okumura N.


The fibrous form of intracellular inclusion bodies in recombinant variant fibrinogen-producing cells is specific to the hapatic fibrinogen storage disease-inducible variant fibrinogen
Int J Hematol,105:758-768 2017(Jun. 30)
Author:Arai S, Ogiwara N, Mukai S, Takezawa Y, Sugano M, Honda T, Okumura N


多項目自動血球分析装置XE2100(Sysmex)のDIFFスキャタグラムにおける側方蛍光低値から発見された異常ヘモグロビン症の4例
医学検査,66:96-102 2017(Mar. 31)
Author:中越りつこ,向井早紀,松田和之,竹澤由夏,新井慎平,菅野光俊,本田孝行, 奥村伸生 中越りつこ,向井早紀,松田和之,竹澤由夏,新井慎平,菅野光俊,本田孝行, 奥村伸生


A novel mutation in the fibrinogen Bβ chain (c.490G>A; End of Exon 3) causes a splicing abnormality and ultimately leads to congenital hypofibrinogenemia
Int. J. Mol. Sci,18:2470(doi:10.3390/ijms18112470) 2017
Author:Taira C, Matsuda K, Arai S, Sugano M, Uehara T, Okumura N.


Congenital dysfibrinogenemia in a Japanese family with Fibrinogen Naples (BβAla68Thr) manifesting as superior sagittal sinus thrombosis
Blood Coag Fibrinolysis,28:580-584 2017
Author:Yoshida S, Kibe T, Matsubara R, Koizumi S, Nara K, Amono K, Okumura N.


A novel frameshift mutation in the fibrinogen γC terminal region, FGG c.1169_1170 del AT, leading to hypofibrinogenemia.
Thromb Res,159:82-85 2017
Author:Nagata K, Arai S, Taira C, Sugano M, Honda T, Okumura N,


Hypodysfibrinogenemia with a heterozygous mutation of γCys326Ser by the novel transversion of TGT to TCT in a patient with pulmonary thromboembolism and right ventricular thrombus
Cardiology(Karger),137:167-172 2017
Author:Ushijima A, Komai T, Matsukawa A, Oikawa K, Morita N, Asai S, Mukai S, Okumura N, Kobayashi Y, Miyachi H.


Genetic analyses of novel compound heterozygous hypodysfibrinogenemia, Tsukuba I: FGG c.1129+62_65 del AATA and FGG c.1299+4 del A
Thromb Res,148:111-117 2016(Dec. 31)
Author:Mukai S, Nagata K, Ikeda M, Arai S, Sugano M, Honda T, Okumura N


関節リウマチ患者における血清中シトルリン化フィブリノゲンおよびその抗体の定量
生物試料分析,39:263-270 2016(Sep. 30)
Author:寺澤文子,石井 亘,樋口由美子,新井慎平,奥村伸生


フィブリノゲン異常症・欠損症に関するコンサルテーションおよび病因解析
臨床検査学教育,8(1):14-19 2016(Mar. 31)
Author:奥村伸生


臨床検査の現場から発信する医学研究、日常検査における異常データから発見された遺伝子異常症とその異常データを生じる原因解明
臨床検査,60:30-35 2016(Jan. 31)
Author:奥村伸生


Differences in the function and secretion of congenital aberrant fibrinogenemia between heterozygous gamma D320G (Okayama II) and gamma Delta N319-Delta D320 (Otsu I)
THROMBOSIS RESEARCH,136(6):1318-1324 2015(Dec.)
Author:Mukai, S; Ikeda, M; Takezawa, Y; Sugano, M; Honda, T; Okumura, N


Novel heterozygous dysfibrinogenemia, Sumida (A alpha C472S), showed markedly impaired lateral aggregation of protofibrils and mildly lower functional fibrinogen levels
THROMBOSIS RESEARCH,135(4):710-717 2015(Apr.)
Author:Ikeda, M; Arai, S; Mukai, S; Takezawa, Y; Terasawa, F; Okumura, N


胆管癌および深部静脈血栓症を伴う続発性クリオフィブリノゲン血症の解析
臨床病理,63:421-425 2015
Author:新井慎平,菅野光俊,本田孝行,亀子文子,寺澤文子,奥村伸生


Recombinant gamma T305A fibrinogen indicates severely impaired fibrin polymerization due to the aberrant function of hole 'a' and calcium binding sites
THROMBOSIS RESEARCH,134(2):518-525 2014(Aug.)
Author:Ikeda, M; Kobayashi, T; Arai, S; Mukai, S; Takezawa, Y; Terasawa, F; Okumura, N


ヘテロ異常血漿フィブリノゲンKyoto V (γF322S)とリコンビナントフィブリノゲンγ322Sのフィブリン重合障害の比較検討
日本検査血液学会雑誌,15:163-170 2014
Author:池田み奈美,新井慎平,向井早紀,稲葉 亨,奥村伸生


異常フィブリノゲンヘテロ接合体 γ鎖Asn308Lys3家系のフィブリノゲン機能比較とハプロタイプ解析異常
医学検査 ,63:133-139 2014
Author:征矢佳輔,新井慎平,竹澤由夏,菅野光俊,寺澤文子,奥村伸生


γ375W fibrinogen-synthesizing CHO cells indicate the accumulation of variant fibrinogen within endoplasmic reticulum
THROMBOSIS RESEARCH,133(1):101-107 2014
Author:Kobayashi, T; Arai, S; Ogiwara, N; Takezawa, Y; Nanya, M; Terasawa, F; Okumura, N


Successdul living-related kidney transplantation in a boy with inherited dysfibrinogenemia
Pediatr Transplant,17:E161- E164 2013
Author:Imamura H, Akioka Y, Asano T, Sugawara N, Ishizuka K, Chikamoto H, Taki M, Terasawa F, Okumura N, Hattori M.


Nonsense-mediated mRNA decay was demonstrated in two hypofibrinogenemias caused by heterozygous nonsense mutations of FGG, Shizuoka III and Kanazawa II
Thromb Res,132:465-470 2013
Author:Soya K, Takezawa Y, Okumura N, Terasawa F


血栓症が強く疑われた患者において発見された異常フィブリノゲン血症ヘテロ患者,Fibrinogen Hamamatsu IIの機能解析(総説)
臨床検査,57:919-925 2013
Author:竹澤由夏,寺澤文子,奥村伸生


siRNA down-regulation of FGA mRNA in HepG2 cells demonstratedthat heterozygous abnormality of the Aalfa-chain gene does not affect the plasma fibrinogen level
Thrombosis Research,131:342-348 2013
Author:Takezawa Y, Matsuda K, Terasawa F, Sugano M, Honda T, Okumura N


Fibrinopeptide A release is necessary for effective B:b interactions in polymerization of variant fibrinogens with impaired A:a interactions
Thrombosis and Haemostasis,109:221-228 2013
Author:Soya K, Terasawa F, Okumura N


Molecular analysis of afibrinogenemic mutations caused by a homozygous FGA1238 bp deletion, and a compound heterozygous FGA1238 bpdeletion and novel FGA c.54+3A>C substitution
INTERNATIONAL JOURNAL OF HEMATOLOGY,96(1):39-46 2012(Jul.)
Author:Takezawa, Y; Terasawa, F; Matsuda, K; Sugano, M; Tanaka, A; Fujiwara, M; Kainuma, K; Okumura, N


Henoch-Schonlein紫斑病罹患時のフィブリノゲン低下により診断に至ったヘテロ型フィブリノゲン欠損症の1例
小児科,53:513-516 2012
Author:清水正樹,奥村伸生,谷内江昭宏


Lateral medullary syndrome in a boy with hereditary dysfibrinogenemia
Brain and Development,34:857-860 2012
Author:Kibe T, Ikeya M. Yokochi K, Okumura N


Hypo/dysfibrinogenemiaが疑われた新規変異フィブリノゲンNagakute (γ305Thr→Ala)とその近傍アミノ酸変異フィブリノゲンの細胞外分泌能の比較
臨床病理,60:831-838. 2012
Author:小林玉宜,竹澤由夏,寺澤文子,奥村伸生


Aα16Arg→His変異によりフィブリノペプチドA放出に異常を有する2家系の異常フィブリノゲン、Toyama、Adachi、の機能解析
臨床病理,60:499-505. 2012
Author:征矢佳輔,竹澤由夏,寺澤文子,奥村伸生


ミスマッチPCR法を用いたSNPs解析による母体血中の胎児DNA検出
臨床病理,60:19-26 2012
Author:小林美和,松田和之,寺澤文子, 奥村伸生,本田孝行,吉川文彦,根津八紘


Application of allele-specific quantitative PCR using genomic DNA to monitor minimal residual disease based on mutant gene levels following allogeneic hematopoietic stem cell transplantation in patients with hematological malignancies: Comparison of mutant levels with autologousDNA percentage by short tandem repeat-PCR.
Clinica Chimica Acta,413:516-519 2012
Author:Taira C, Matsuda K, Saito S, Sakashita K, Sugano M, Okumura N, Honda T.


Heterozygous Bβ C-terminal 12 amino acid-elongation variant,BβX462W (Kyoto VI), showed dysfibrinogenemia.
Blood Coagulation and Fibrinolysis,23(1):87-90 2012
Author:Okumura N , Terasawa F, Takezawa Y, Hirota-Kawadobora M, Inaba T, Fujita N, Saito M, Sugano M, Honda T.


フィブリノゲンの細胞内生成・分泌におけるBβ鎖D領域454-458残基の重要性
臨床病理,59:741-748 2011
Author:寺澤文子, 竹澤由夏, 廣田(川戸洞)雅子, 奥村伸生


Quantitative monitoring of single nucleotide mutation by allele-specific quantitative PCR can be used for assessment of minimal residual disease in patients with hematological malignancies throughout their clinical course.
Clinica Chimica Acta,412:53-58 2011
Author:Taira C, Matsuda K, Kamijyo Y, Sakashita K, Ishida F, Kumagai T, Yamauchi K, Okumura N, Honda T.


Spontaneous regression of the inhibitor against the coagulation factor XIII A subunit in acquired factor XIII deficiency.
Thrombosis and Haemostasis,104:1284-1285 2010
Author:Ishida F, Okubo K, Ito T, Okumura N, Souri M, Ichinose A.


フィブリノゲンγ鎖C末端D領域(γ313~345)に変異を有するフィブリノゲンのフィブリノゲン組み立て・分泌における機能
臨床病理,58:772-778 2010
Author:寺澤文子, 上條由夏, 藤原祝子, 奥村伸生.


A C-terminal amino acid substitution in the gamma-chain caused by a novel heterozygous frameshift mutation (Fibrinogen Matsumoto VII) results in hypofibrinogenaemia.
Thrombosis and Haemostasis,104(2):213-223 2010
Author:Fujihara N, Haneishi A, Yamauchi K, Terasawa F, Ito T, Ishida F, Okumura N.


臨床検査の現場と連携した教育・研究体制の実現―信州大学医学部保健学科の試みと今後の課題―
臨床検査学教育.(総説),2:1-7 2010
Author:奥村伸生, 熊谷俊子, 山内一由, 本田孝行.


In vitro transcription of compound heterozygous hypofibrinogenemia Matsumoto IX; first identification of FGB IVS6 deletion of 4 nucleotides and FGG IVS3-2A>G causing abnormal RNA splicing
Clinica Chimica Acta,411(17-18):1325-1329 2010
Author:Fumiko Terasawa, Yuka Kamijyo, Noriko Fujihara, Kazuyoshi Yamauchi, Toshiko Kumagai, Takayuki Honda, Satoshi Shigematsud and Nobuo Okumura


Analysis of plasmin generation and clot lysis of plasma fibrinogen purified from a heterozygous dysfibrinogenemia, betaGly15Cys (Hamamatsu II).
Blood Coagulation and Fibrinolysis,20(8):726-732 2009
Author:Kamijyo Y, Hirota-Kawadobora M, Yamauchi K, Terasawa F, Honda T, Ikeya M, Okumura N.


Impaired protofibril formation in fibrinogen gammaN308K is due to altered D:D and “A:a” interactions.
Biochemistry,48:8656-8663 2009
Author:Bowley SR, Okumura N, Lord ST.


異常フィブリノゲンへテロ接合体γ鎖Arg275Cys 2家系とγ鎖Arg275His 3家系のフィブリノゲン機能解析とハプロタイプ解析.
臨床病理,57:651-658 2009
Author:上條由夏, 廣田(川戸洞)雅子, 藤原祝子, 若林早紀, 松田和之, 山内一由, 寺澤文子, 奥村伸生, 本田孝行.


Recombinant variant fibrinogens substituted at residues gamma326Cys and gamma339Cys demonstrated markedly impaired secretion of assembled fibrinogen.
Thrombosis Research,124:368-372 2009
Author:HaneishiA, Terasawa F, Fujihara N, Yamauchi K, Okumura N, Katsuyama T.


フィブリノゲン異常症と欠損症から明らかになった フィブリノゲンの機能異常と産生異常
信州医学雑誌,57(5):145-154 2009
Author:奥村伸生、寺澤文子
Keywords:fibrinogen、dysfibrinogenemia、afibrinogenemia、hypofibrinogenemia、fibrin polymerization、フィブリノゲン、フィブリノゲン異常症、無フィブリノゲン血症(フィブリノゲン欠損症)、低フィブリノゲン血症、フィブリン重合反応


Citrullinated fibrinogen shows defects in FPA and FPB release and fibrin polymerization catalyzed by thrombin.
Clinica Chimica Acta,401:119-123 2009
Author:Okumura N, Haneishi A, Terasawa F.


特定機能病院のための臨床検査技師教育
臨床病理,56:612-616 2008
Author:奥村伸生


Polymerization-defective fibrinogen variant gammaD364A binds knob “A” peptide mimic
Biochemistry,37:8607-8613 2008
Author:Bowley SR, Merenbloom BK, Okumura N, Betts L, Heroux A, Gorkun OV, Lord ST


血液凝固・線溶系物質-メタボリックシンドロームにおけるPAI-1を中心に-
臨床化学,37:272-282 2008
Author:奥村伸生, 寺澤文子


フィブリノゲン
検査と技術,36:707-715 2008
Author:奥村伸生, 寺澤文子, 廣田(川戸洞)雅子, 藤原祝子


Sialic acid moiety of apolipoprotein E3 at Thr194affects its interaction with ?-amyloid1-42 peptides
Clinica Chimica Acta,388:123-129 2008
Author:Sugano M, Yamauchi K, Kawasaki K, Tozuka M, Fujita K, Okumura N, Ota H


ウエスタンブロット法によるDダイマー測定試薬の反応性の比較検討
臨床病理,56:449-454 2008
Author:若林早紀, 石川伸介, 廣田(川戸洞)雅子, 藤原祝子, 上條由夏, 山内一由, 寺澤文子, 奥村伸生, 勝山 努


B:b interactions are essential for polymerization of variant fibrinogens with impaired holes ‘a’
Journal of Thrombosis and Haemostasis,5:2352-2359 2007
Author:Okumura N, Terasawa F, Haneishi A,Fujihara N,Hirota-Kawadobora M, Yamauchi K, Ota H, Lord ST


日常検査で遭遇する低フィブリノゲン異常症・欠損症の凝固スクリーニング検査結果の検討
臨床病理,55:989-995 2007
Author:廣田(川戸洞)雅子, 石川伸介, 藤原祝子, 若林早紀, 上條由夏, 山内一由, 寺澤文子, 奥村伸生, 勝山 努


In vitro expression of a-thalassaemia gene (IVS1-1G>C) reveals complete inactivation of the normal 5’splice site and alternative aberrant RNA splicing.
Annals of Clinical Biochemistry,44:573-578 2007
Author:Fujihara N, Yamauchi K, Hirota-Kawadobora M, Ishikawa S, Tozuka M, Ishii E, Katsuyama T, Okumura N, Taniguchi S


赤血球沈降速度
検査と技術,35:337-342 2007
Author:奥村伸生, 藤原祝子


変異フィブリノゲンKyoto IV(Bb鎖コイルドコイル領域Ser111欠損)の発見と文献的考察
日本検査血液学会雑誌,7:394-400 2006
Author:中西加代子,森 尚子,石田敦巳,藤井葉子,志賀修一,斎藤 潤,奥村伸生,一山 智


Co-localization of TFF2 with gland mucouscell mucin in gastric mucous cellsand in extracellular mucous gel adherent to normal and damaged gastric mucosa
HISTOCHEMISTRY AND CELL BIOLOGY,126:617-625 2006
Author:Ota, H; Hayama, M; Momose, M; El-Zimaity, HMT; Matsuda, K; Sano, K; Maruta, F; Okumura, N; Katsuyama, T


An immunoglobulin A1 that inhibits lactate dehydrogenaseactivity, with reversal of inhibition by addition of NADH
Annals of Clinical and Laboratory Science,36:461-468 2006
Author:Fujita K, Sato H, Kameko F, Terasawa F, Okumura N, Sugano M, Yamauchi K, Maekawa M, Sakurabayashi I.


Invitro fibrin clot formation and fibrinolysis using heterozygous plasma fibrinogen from gAsn319,Asp320 deletion dysfibrinogen, Otsu I.
Thrombosis Research,118:651-661 2006
Author:Terasawa F, Kani S, Hongo M, Okumura N.


Analysis of fibrinogen variants at gamma 387Ile shows that the side chain of gamma 387 and the tertiary structure of the gamma C-terminal tail are important not only for assembly and secretion of fibrinogen but also for lateral aggregation of protofibrils and XIIIa-catalyzed gamma-gamma dimer formation
BLOOD,108:1887-1894 2006
Author:Kani, S; Terasawa, F; Yamauchi, K; Tozuka, M; Okumura, N


Mechanism of IgA-albumin complex formation that affectsthe fructosamine assay
Journal of Electrophoresis,50:19-23 2006
Author:Fijita K, Kameko F, Kato Y, Fukushima M, Okumura N, Terasawa F, Sugano M, YamauchiK, Sato H, Kameko M, Sakurabayashi I.


A novel variant fibrinogen, deletion of B beta 111Ser in coiled-coil region, affecting fibrin lateral aggregation
CLINICA CHIMICA ACTA,365:160-167 2006
Author:Okumura, N; Terasawa, F; Hirota-Kawadobora, M; Yamauchi, K; Nakanishi, K; Shiga, S; Ichiyama, S; Saito, M; Kawai, M; Nakahata, T


遺伝子疾患の遺伝子検査
医学検査,54:1477-1484 2005
Author:奥村伸生


In vitro expression demonstrates impaired secretion of the gammaAsn 319, Asp320 deletion variant fibrinogen
Thromb Haemost,94:53-59 2005
Author:Kani S, Terasawa F, Lord ST, Tozuka M, Ota H, Okumura N, Katsuyama T


Functional analysis of recombinant Bbeta15C and Bbeta15A fibrinogens demonstrates that Bbeta15G residue plays important roles in FPB release and in lateral aggregation of protofibrils
Journal of Thrombosis and Haemostasis,3:983-990 2005
Author:M.Hirota-Kawadobora, S.Kani, F.Terasawa, N.Fujihara, K.Yamauchi, M.Tozuka, N.Okumura


Residue gamma153Cys is essential for the formationof the complexes Aalpha-gamma and Bbeta-gamma, assembly intermediates for the Aalpha-Bbeta-gamma complex and intact fibrinogen
Clinica Chimica Acta,353:157-164 2005
Author:Fumiko Terasawa, Kiyotaka Fujita, Nobuo Okumura


フィブリノゲン欠損症とフィブリノゲンの機能
Medical Technology,32:122-123 2004
Author:奥村伸生


Characterization of factor XII Tenri, a rare CRM-negative factor XII deficiency
Annals of Clinical and Laboratory Science,34:218-225 2004
Author:Noriko Fijihara, Minoru Tozuka, Kazuyoshi Yamauchi, Ichiro Ueno, Nobuyuki Urasawa, Shinsuke Ishikawa, Masako Hirota-Kawadobora, Nobuo Okumura, Hiroya Hidaka, Tsutomu Katsuyama


Comparison of thrombin-catalyzed fibrin polymerization and factor XIIIa-catalyzed cross-linking of fibrin among three recombinant variant fibrinogens, gamma275C, gamma275H, and gamam275A
Journal of Thrombosis and Haemostasis,2:1359-1367 2004
Author:M.Hirota-Kawadobora, F.Terasawa, T.Suzuki, M.Tozuka, K.Sano, N.Okumura


Substitution of the gamma-chain Asn308 disturbs the D:D interface affecting fibrin polymerization, fibrinopeptide B release, and FXIIIa-catalyzed cross-linking
Blood,103:4157-4163 2004
Author:Nobuo Okumura, Oleg V. Gorkun, Fumiko Terasawa, Susan T. Lord


Recombinant fibrinogen, gamma275Arg->Cys, exhibits formation of disulfide bond with cysteine and severely impaired D:D interactions
Journal of Thrombosis and Haemostasis,2:468-475 2004
Author:S.Ishikawa, M.Hirota-Kawadobora, M.Tozuka, K.Ishii, F.Terasawa, N.Okumura


フィブリノゲン欠損症とフィブリノゲンの機能
信州医学雑誌,51:237-238 2003
Author:奥村伸生


The Aalpha-chain 6Ile/Val polymorphism is not associated with plasma fibrinogen levels in Japanese
Thrombosis Research,112:257-259 2003
Author:Fumiko Terasawa, Masako Hirota-Kawadobora, Hikaru Kobayashi, Hiroshi Saito, Minoru Tozuka, Nobuo Okumura


Sequence gamam377-395(P2), but not gamma190-202(P1),is the binding site for the alphaMI-domain of integrin alphaMbeta2 inthe gammaC-domain of fibrinogen
Biochemistry,42:9365-9373 2003
Author:Tatiana P. Ugarova, Valeryi K. Lishko, Nataly P. Podolnikova, Nobuo Okumura, et al (5)


Novel beta-thalassemia trait (IVS I-1 G->C) in a Japanese family
American Journal of Hematology,72:64-66 2003
Author:Noriko Fijihara, Minoru Tozuka, Ichiro Ueno, Kazuyoshi Yamauchi, Ritsuko Nakagoshi, Shinsuke Ishikawa, Masako Hirota, Nobuo Okumura, Eizaburo Ishii, Tsutomu Katsuyama


Serotonin receptor antagonist inhibits monocrotaline-induced pulmonary hypertension and prolongs survival in rats
Cardiovascular Research,60:692-699 2003
Author:Hironaka E, Hongo M, Sakai A, Mawatari E, Ushiyama Y, Terasawa F, Okumura N,Yamazaki A, Yazaki Y, Kinoshita O, Kubo


Identification and properties of glycated monoclonal IgA that Affect the fructosamine assay.
Clinical Chemistry,49:805-808 2003
Author:Fujita K, Curtiss LK, Sakurabayashi I, Kameko F, Okumura N, Terasawa F, Tozuka M, Katsuyama


Fibrinogen Otsu I: A γAsn319, Asp320 deletiondysfibrinogen identified in an asymotomatic pregnant woman.
Thrombosis and Haemostasis,90:757-758 2003
Author:Terasawa F, Hogan KA, Kani S, Hirose M, Eguchi Y, Noda Y, Hongo M, Okumura N


Immunohistochemical lacalization of sodium-potassium ATPase in human normal stomach and gastric adenocarcinomas.
Histochem Cell Biol,119:317-322 2003
Author:Kobayashi C, Hayama M, Harada O, Terasawa F, Okumura N, Sugiyama A, Ota H


Fibrinogens Kosai and Ogasa: Bβ15Gly-Cys(GGT-TGT) substitution associated with impairment of fibrinopeptide B release and lateral aggregation.
J Thrombosis and Haemostasis,1:275-283 2003
Author:Hirota-Kawadobora M, Terasawa F, Yonekawa O, Sahara N, Shimizu E, Okumura N, Katsuyama T, Shigematsu


DNAシークエンス法.誰でもわかる遺伝子検査.II.各論ー遺伝子検査はどういうときに必要なのか2.技術編ー遺伝子検査を活用するための知識3)分析法/解析法.
検査と技術,30:976-980 2002
Author:奥村伸生,寺澤文子


Evidence that heterodimers exist in the fibrinogenMatsumoto II (γ308N-K) proband and participate infibrin fiber formation.
Thrombosis Reseach,107:157-162 2002
Author:Okumura N, Terasawa F, Kiyotaka Fujita, Noriko Fujiwara, Minoru Tozuka, Chang-Sung Koh


Quantitative RT-PCR analysis demonstrates that synthesis of the recombinant fibrinogen is dependent on the transcription and synthesis of g-chain
Clinica Chimica Acta,319:67-73 2002
Author:Hirota-Kawadobora M, Tozuka M, Yamauchi K,Hidaka E, Ueno I, Sugano M, Terasawa F, Okumura N,Katuyama T, Shigematsu H


Analysisof fibrinogen gamma-chain truncations shows the C-terminus, particularly gammaIle387, is essential for assembly and secretion of this multichain protein
Blood,99:3654-3660 2002
Author:Okumura N, Terasawa F, Tanaka H, Hirota M, Ota H, Kitano K, Kiyosawa K, Lord ST


Predominant apolipoprotein J exists as lipid-poor mixtures in cerebrospinal fluid
Ann Clin Lab Sci,32:369-376 2002
Author:Suzuki T, Tozuka M, Kazuyoshi Y, Sugano M, Nakabayashi T, Okumura N, Hidaka H, Katsuyama T, Higuchi K


フィブリン重合反応におけるD-E ('a-A')結合異常の解析 ーD ドメイン('a')に変異を有するMatsumoto I (γ364Asp->His)とE ドメイン('A')に変異を有するMatsumoto V(Aα19Arg->Gly)の比較.
信州大学医療技術短期大学部紀要,27:57-66 2001
Author:寺澤文子,田中 仁,廣田(川戸洞)雅子,石川伸介,奥村伸生


Hypofibrinogenemia associated with a heterozygous C->T nucleotide substitution at position -1138 bp of the 5'-flanking region of the fibrinogen Aα-chain.
Annals of New York Academy of Science,936:526-530 2001
Author:Okumura N, Terasawa F, Yonekawa O, Hamada E, Kaneko H


The formation of β fibrin requires a functional a site.
Annals of New York Academy of Science,936:219-222 2001
Author:Hogan KA, Bolliger B, Okumura N, Lord ST


Polymerization site a function dependence on structural integrity of its nearby calcium binding site.
Annals of New York Academy of Science,936:205-209 2001
Author:Lounes KC, Okumura N, Hogan KA, Ping L, Lord ST


FDP-EとFDP-Dダイマー値の乖離を認めたアメリカ鉤虫感染症の一例.
臨床病理,49:82-86 2001
Author:中越りつこ,小口弘子,石井栄三郎,石川伸介,樋口由美子,村松紘一,奥村伸生,小木曽嘉文


Fibrinogen Matsumoto V: a variant with Aα19 Arg->Gly(AGG->GGG). Comparison between fibrin polymerization stimulated by thrombin or reptilase and fibrin monomer polymerization.
Thrombosis and Haemostasis,85:108-113 2001
Author:Tanaka H, Terasawa F, Ito T,Tokunaga S, Ishida F, Kitano K, Kiyosawa K, Okumura N


電気泳動法を用いたフィブリノゲン異常症および欠損症の解析.
生物物理化学,44:283-288 2000
Author:奥村伸生,寺澤文子


Analysis of hemoglobin and globin chain variants by a commonly used capillary isoelectric focusing method.
Electrophoresis,21(14):3016-3019 2000
Author:Sugano M, Hidaka H, Yamauchi K, Nakabayahsi T, Higuchi Y, Fujita K, Okumura N, Ushiyama Y, Tozuka M, Katsuyama T


Identification of a dysfibrinogen, the substitution of γ308Asn(AAT) to Lys(AAG), using coagulation tests, immunoblot analysis, and allele-specific polymerase chain reaction.
Clinica Chimica Acta,295(1-2):77-85 2000
Author:Terasawa F, Fujita K, Tozuka M, Ota H, Katsuyama T, Okumura N


A functional assay suggests that heterodimers exist in two C-terminal γ-chaindysfibrinogens; Matsumoto I and Vlissingen/Frankfurt IV.
Thrombosis and Haemostasis,83(4):592-597 2000
Author:Hogan KA, Lord ST, Okumura N, Terasawa F, Galanakis DK, Scharrer I, Gorkun OV


Difference in electrophoretic mobility and plasmic digestion profile between four recombinant fibrinogens, gamma 308K, gamma 308I, gamma 308A, and wild type (gamma 308N)
ELECTROPHORESIS,21(12):2309-2315 2000
Author:Okumura, N; Terasawa, F; Fujita, K; Tozuka, M; Ota, H; Katsuyama, T


凝固第Ⅴ因子阻害物質の出現を見た一症例.
臨床病理,47:971-975 1999
Author:中越りつこ,高宮 脩,中田伸司,石川伸介,樋口由美子,奥村伸生,小木曽嘉文


Genetic analyses in homozygous and heterozygous variant of lactate dehydrogenese-B(H) subunit-LD-B Matsumoto I and II (LD-B W323R)-.
Clinica Chimica Acta,287(1-2):163-171 1999
Author:Okumura N, Terasawa F, Ueno I, Oki K, Yamauchi K, Hidaka H, Tozuka M, Okura M, Katsuyama T


Fibrinogen Matsumoto III: a variant with γ275Arg→Cys(CGC→TGC) -comparison of fibrin polymerization with those of Matsumoto I (γ364Asp→His) and Matsumoto II (γ308Asn→Lys).
Thrombosis and Haemostasis,81(5):763-766 1999
Author:Terasawa F, Okumura N,Higuchi Y, Ishikawa S, Tozuka M, Kitano K, Katsuyama T


市販DNA抽出キットを用いたDNA抽出法の比較検討.
医学検査,48:856-859 1999
Author:行正信康,上野一郎,横田浩充, 風間文智, 奥村伸生


Hypofibrinogenemia associated with a heterozygous missense mutation γ153Cys to Arg (Matsumoto IV): In vitro expression demonstrates defective secretion of the variant fibrinogen.
Blood,94(12):4122-4131 1999
Author:Terasawa F, Okumura N, Kitano K, Hayashida N, Shimosaka M, Okazaki M, Lord ST


Fibrinogen Matsumoto IV: A heterozygous fibrinogen deficiency caused by a missense mutation ofr153Cys toArg
THROMBOSIS AND HAEMOSTASIS,:42-42 1999
Author:Terasawa, F; Okumura, N; Lord, ST


小型球形ウイルス(SRSV)検出のためのウイルスRNA抽出法とRT-PCR法の迅速改良法.
医学検査,48:266-270 1999
Author:上野一郎, 日高恵以子,奥村伸生,赤羽美智子,小林宏正,野竹重幸, 柳沢英二


Comparison of functions between recombinant variant fibrinogens, r308K, r308I and r308A
THROMBOSIS AND HAEMOSTASIS,:321-321 1999
Author:Okumura, N; Terasawa, F; Lord, ST


Flow Cytometryを用いた血小板活性化能測定の基礎的検討とその評価法
臨床病理,46:605-610 1998
Author:石川伸介, 戸塚 実, 廣田雅子, 佐々木由美子, 奥村伸生, 降旗謙一, 勝山 努


生化学検査用血清を用いたDダイマー測定の検討
臨床検査,42:1167-1171 1998
Author:中越りつこ, 戸塚 実, 北和田修介, 荒井園子, 村松紘一, 奥村伸生


PlasminogenKanagawa-I, a novel missense mutation, is caused by the amino acid substitution G732R
British J of Haematology,103(3):867-870 1998
Author:Higuchi Yumiko, Furihata Kenichi, Ueno Ichiro, Ishikawa Shinshuke, Okumura Nobuo, Tozuka Minoru, Sakurai Noriko


Characterization of hypertriglyceridemia induced by L-asparaginase therapy for acute lymphoblastic leukemia and malignant lymphoma
ANNALS OF CLINICAL AND LABORATORY SCIENCE,27(5):351-357 1997
Author:Tozuka, M; Yamauchi, K; Hidaka, H; Nakabayashi, T; Okumura, NS; Katsuyama, T


Megakaryocytes derived from CD34-positive cord blood cells produce interleukin-8
BRITISH JOURNAL OF HAEMATOLOGY,99(3):509-516 1997
Author:Higuchi, T; Koike, K; Sawai, N; Mwamtemi, HH; Takeuchi, K; Shiohara, M; Kikuchi, T; Yasui, K; Ito, S; Yamagami, O; Sasaki, Y; Okumura, N; Kato, T; Miyazaki, H; Ikeda, M; Yamada, M; Komiyama, A


Aberrant growth of granulocyte-macrophage progenitors in juvenile chronic myelogenous leukemia in serum-free culture
EXPERIMENTAL HEMATOLOGY,24(2):116-122 1996
Author:Sawai, N; Koike, K; Ito, S; Okumura, N; Kamijo, T; Shiohara, M; Amano, Y; Tsuji, K; Nakahata, T; Oda, M; Okamura, J; Kobayashi, M; Komiyama, A


Influence of apolipoprotein E phenotypes on total serum cholesterol in Japanese children
CLINICA CHIMICA ACTA,247(1-2):175-180 1996
Author:Tozuka, M; Ohta, H; Hidaka, H; Okumura, N; Furihata, K; Katsuyama, T


Chemotactic and chemokinetic activities of stem cell factor on murine hematopoietic progenitor cells
BLOOD,87(10):4100-4108 1996
Author:Okumura, N; Tsuji, K; Ebihara, Y; Tanaka, I; Sawai, N; Koike, K; Komiyama, A; Nakahata, T


Fibrinogen Matsumoto I: A gamma 364 Asp->His (GAT->CAT) substitution associated with defective fibrin polymerization
THROMBOSIS AND HAEMOSTASIS,75(6):887-891 1996
Author:Okumura, N; Furihata, K; Terasawa, F; Nakagoshi, R; Ueno, I; Katsuyama, T


A case of immunoglobulin A-lambda conjugated with lactate dehydrogenase-5 isoenzyme, causing an extremely high enzyme activity in serum
CLINICAL CHEMISTRY,42(8):1288-1290 1996
Author:Tozuka, M; Hidaka, H; Okumura, N; Ichikawa, T; Furibata, K; Katsuyama, T


Fibrinogen Matsumoto II: gamma(308)Asn->Lys(AAT->AAG) mutation associated with bleeding tendency
BRITISH JOURNAL OF HAEMATOLOGY,94(3):526-528 1996
Author:Okumura, N; Furihata, K; Terasawa, F; Ishikawa, S; Ueno, I; Katsuyama, T


PREVENTION OF HEPATIC-ARTERY THROMBOSIS IN PEDIATRIC LIVER-TRANSPLANTATION
TRANSPLANTATION,60(10):1109-1112 1995
Author:HASHIKURA, Y; KAWASAKI, S; OKUMURA, N; ISHIKAWA, S; MATSUNAMI, H; IKEGAMI, T; NAKAZAWA, Y; MAKUUCHI, M


Fibrinogen Matsumoto II: A gamma 308ASN->Lys(AAT->AAG) mutation associated with defective fibrin polymerization.
BLOOD,86(10):3520-3520 1995
Author:Okumura, N; Terasawa, F; Ueno, I; Kaysuyama, T; Furihata, K


CELL-SURFACE ANTIGEN EXPRESSION IN HUMAN ERYTHROID PROGENITORS - ERYTHROID AND MEGAKARYOCYTIC MARKERS
LEUKEMIA&LYMPHOMA,13(5-6):401-409 1994
Author:NAKAHATA, T; OKUMURA, N


GENETIC-ANALYSIS OF FIBRINOGEN MATSUMOTO - A POINT MUTATION IN EXON-9 OF GAMMA-CHAIN RESPONSIBLE FOR DEFECTIVE FIBRIN POLYMERIZATION
BLOOD,84(10):A60-A60 1994
Author:TERASAWA, F; OKUMURA, N; UENO, I; HIDAKA, E; FURUWATARI, C; YAN, J; KATSUYAMA, T; FURIHATA, K


CHANGES IN CELL-SURFACE ANTIGEN EXPRESSIONS DURING PROLIFERATION AND DIFFERENTIATION OF HUMAN ERYTHROID PROGENITORS
EXPERIMENTAL HEMATOLOGY,20(6):793-793 1992
Author:OKUMURA, N; NAKAHATA, T


CHANGES IN CELL-SURFACE ANTIGEN EXPRESSIONS DURING PROLIFERATION AND DIFFERENTIATION OF HUMAN ERYTHROID PROGENITORS
BLOOD,80(3):642-650 1992
Author:OKUMURA, N; TSUJI, K; NAKAHATA, T


COAGULATION ABNORMALITIES FOLLOWED ORTHOTOPIC LIVING RELATED LIVER-TRANSPLANTATION
THROMBOSIS AND HAEMOSTASIS,65(6):1090-1090 1991
Author:SAITO, H; OKUMURA, N; HARADA, H; KUDO, M; MAKUUCHI, M; FURUTA, S


INTERLEUKIN-6 SUPPORTS HUMAN MEGAKARYOCYTIC PROLIFERATION AND DIFFERENTIATION INVITRO
BLOOD,78(8):1969-1974 1991
Author:IMAI, T; KOIKE, K; KUBO, T; KIKUCHI, T; AMANO, Y; TAKAGI, M; OKUMURA, N; NAKAHATA, T


その他
大学院教育とリンクした臨床検査技師卒後教育制度の確立~人材確保と育成を指向した新たな戦略~
臨床検査学教育 , 1:133-137 2009
Author:山内一由, 奥村伸生, 太田浩良,本田孝行

共同研究等希望テーマ
臨床検査学 , 臨床化学
フィブリノゲン・フィブリン , 血栓止血学
シトルリン化フィブリノゲン・フィブリンとその自己抗体 , 臨床免疫学
研究費
科学研究費補助金(研究代表者)
2017 - 2019 , フィブリノゲン機能異常症例の解析からフィブリン重合機序の詳細を解明する研究 , 基盤研究(C)
2014 - 2016 , 肝硬変を引き起こすフィブリノゲン低下症の鑑別法の開発と分子生物学的発生機序解明 , 基盤研究C
2010 - 2012 , シトルリン化フィブリノゲン・フィブリンの溶解性に関する研究 , 基盤(C)
2007 - 2008 , シトルリン化フィブリノゲンの凝固・線溶機能異常とその病因との関係 , 基盤研究(C)
2004 - 2006 , フィブリノゲンの組み立て・分泌におけるBb鎖・g鎖の機能の相違に関する研究 , 基盤研究(C)

科学研究費補助金(研究分担者)
2013 - 2015 , 血漿および関節液中のシトルリン化フィブリノゲンとその抗体定量法の確立. , 科学研究費 基盤研究C

その他
2006 - 2007 , リコンビナントフィブリノゲンを用いたフィブリノゲン-フィブリン転換の分子メカニズムの解析とその正常機能を抑制する合成ペプチドの検索
2002 - 2002 , リコンビナントヒト正常・異常フィブリノゲンを用いたフィブリン繊維形成機序の解析と合成ペプチドによるその抑制
1998 - 1999 , 異常フィブリノゲンMataumotoIVの遺伝子導入による原因の研究
1997 - 1997 , リコンビナント異常フィブリノゲンを用いたフィブリン凝集能の解析
研究諸活動
学術専門雑誌等編集
2009- , 臨床検査学教育(編集委員)
2003- , Clinica Chimica Acta (Editorial Board)
2008-2011 , 臨床病理(編集委員)
1998-2007 , 医学検査(査読委員)

在外研究員等
1996-1997 , 長期在外研究員(乙) , The University of North Carolina

研究者招聘
2003-2003 , 日本学術振興会外国人招へい研究者(短期) , Professor of The University of North Carolina

教育活動実績

授業等
2010 , 後期 , 医療生命科学演習B , 大学院博士後期課程
2009 , 前期 , 酵素検査学実習
2009 , 前期 , 体液性免疫検査技術学実習
2009 , 前期 , 細胞性免疫検査技術学実習
2009 , 前期 , 医療生命科学特講 , 大学院博士後期課程
2009 , 後期 , 免疫検査学
2009 , 後期 , 分離分析技術学特論
2009 , 後期 , 健康食品学
2008 , 前期 , 生体分子情報検査学演習 , 大学院博士前期課程
2007 , 前期 , 病態検査解析学 , 大学院博士前期課程
2007 , 前期 , 生体分子情報検査学特論 , 大学院博士前期課程
2007 , 後期 , 臨床化学分析学
2006 , 前期 , 臨地実習
2006 , 後期 , 卒業研究
2004 , 前期 , 病態化学検査学