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堀江 智明  ホリエ トモアキ

教員組織学術研究院(繊維学系)電話番号0268-21-5561
教育組織繊維学部 応用生物科学科FAX番号0268-21-5561
職名教授メールアドレスhorie@shinshu-u.ac.jp
住所〒386-8567 長野県上田市常田3-15-1ホームページURL

プロフィール

研究分野
植物分子生物学
植物生理学
植物細胞工学
現在の研究課題
野生イネ・耐塩性イネからの耐塩性関連遺伝子の発掘
キーワード:野生イネ , 耐塩性イネ
植物の耐塩性機構の解明
キーワード:耐塩性 , イネ , シロイヌナズナ , ナトリウム輸送 , カリウム輸送
イネHKT輸送体の塩ストレス下での生理機能の解明と耐塩性イネ栽培種の育種技術の開発
キーワード:耐塩性 , イネ , タンパク質工学 , 分子育種
所属学会
所属学会
日本育種学会
日本植物生理学会
日本生物工学会
農業食料工学会
学歴
出身大学院
2001 , 奈良先端科学技術大学院大学
1998 , 奈良先端科学技術大学院大学

出身学校・専攻等(大学院を除く)
1996 , 弘前大学

取得学位
バイオサイエンス博士 , 奈良先端科学技術大学院大学
バイオサイエンス修士 , 奈良先端科学技術大学院大学
理学士 , 弘前大学
受賞学術賞
2012 , 第20回生物工学論文賞 , Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells
研究職歴等
研究職歴
2020- , 信州大学繊維学部 応用生物科学科 教授(現在に至る)
2010- , 信州大学繊維学部 応用生物学系 生物資源・環境科学課程 准教授
2007- , 岡山大学資源植物科学研究所 分子生理機能解析グループ 特別契約職員助教

留学歴
2019- , タスマニア大学 土壌・食料学科 客員研究員
2006- , カルフォルニア大学サンディエゴ校 生物科学科 スタッフ研究員
2001- , カルフォルニア大学サンディエゴ校 生物科学科 ポストドクター研究員

研究活動業績

研究業績(著書・
発表論文等)
書籍等出版物
塩ストレスと耐塩性, 植物学の百科事典 , 428-429
丸善出版 2016(Jun. 30)
Author:堀江智明


Chapter 23: Potassium and sodium transporters: improving salinity tolerance in plants, Improving Crop Resistance to Abiotic Stress , 523-542
Wiley-VCH Verlag GmbH & Co., Germany 2012
Author:Yamaguchi, T., Uozumi, N. and Horie, T.


植物のストレスへの応答~乾燥および塩, 植物分子生理学入門 , 199-209
1999(Jun.)
Author:吉田和哉、堀江智明、仲山英樹


論文
Mechanisms Activating Latent Functions of PIP Aquaporin Water Channels via the Interaction between PIP1 and PIP2 Proteins.
Plant and Cell Physiology,62(1):92-99 2021(Mar. 25)
Author:Shibasaka M, Horie T, Katsuhara M.


Functions and structure of roots and their contributions to salinity tolerance in plants.
Breeding Science,71(1):89-108 2021(Mar. 08)
Author:Karahara I, Horie T


A survey of barley PIP aquaporin ionic conductance reveals Ca2+-sensitive HvPIP2;8 Na+ and K+ conductance.
International Journal of Molecular Sciences,21(19):7135 2020(Sep. 27)
Author:Tran STH, Horie T, Imran S, Qiu J, McGaughey S, Byrt CS, Tyerman SD, Katsuhara M.


営農型太陽光発電のパネル制御が水稲栽培に及ぼす影響
農業食料工学会誌,82(4):380-389 2020(Jul. 01)
Author:合原亮一・高橋伸英・加藤茂春・堀江智明


Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots.
International Journal of Molecular Sciences,21(14):4882 2020(Jul.)
Author:Al Nayef M, Solis C, Shabala L, Ogura T, Chen Z, Bose J, Maathuis FJM, Venkataraman G, Tanoi K, Yu M, Zhou M, Horie T, Shabala S.


Doing 'business as usual' comes with a cost: evaluating energy cost of maintaining plant intracellular K+ homeostasis under saline conditions
NEW PHYTOLOGIST,225(3):1097-1104 2020(Feb.)
Author:Rubio F, Nieves-Cordones M, Horie T, Shabala S.


Expression and Ion Transport Activity of Rice OsHKT1;1 Variants
PLANTS-BASEL,9(1) 2020(Jan.)
Author:Imran S, Horie T, Katsuhara M.


Energy costs of salinity tolerance in crop plants
NEW PHYTOLOGIST,221(1):25-29 2019(Jan.)
Author:Tyerman SD Munns R FrickeW Arsova B; Barkla B; Bose J; Bramley H; Byrt C; Chen Z; Colmer TD; Cuin T; Day DA; Foster KJ; Gilliham M; Henderson SW; Horie T; Jenkins CLD; Kaiser BN; Katsuhara M; Plett D; Miklavcic SJ; Roy SJ; Rubio F; Shabala S; Shelden M; Soole K; Taylor NL; Tester M; Watt M; Wege S; Wegner LH; Wen Z


High-Affinity K+ Transporters from a Halophyte, Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis, X. laevis Oocytes and Yeast
PLANT AND CELL PHYSIOLOGY,60(1):176-187 2019(Jan.)
Author:Tada Y, Endo C, Katsuhara M, Horie T, Shibasaka M, Nakahara Y, Kurusu T.


T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress
International Journal of Molecular Sciences,19:235 2018
Author:Oda, Y., Kobayashi, NI., Tanoi, K., Ma, JF., Itou, Y., Katsuhara, M., Itou, T., Horie, T.


Genomics, physiology, and molecular breeding approaches for improving salt tolerance
Annual Review of Plant Biology,68:405-434 2017
Author:Ismail, AM., Horie, T.


A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice
Plant Physiology,174:1837-1849 2017
Author:Chen, ZC., Yamaji, N., Horie, T., Che, J., Li, J., An, G., Ma, JF.


OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice
The Plant Journal,91:657-670 2017
Author:Kobayashi, NI., Yamaji, N., Yamamoto, H., Okubo, K., Ueno, H., Costa, A., Tanoi, K., Matsumura, H., Fujii-Kashino, M., Horiuchi, T., Nayef, MA., Shabala, S., An, G., Ma, JF., Horie, T.


Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport
Physiologia Plantarum,159(1):120–128 2017
Author:Rhee,J., Horie, T., Shizuka, S., Nakahara, Y., Maki, K.


OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress
BMC Plant Biology,16:22 2016
Author:Suzuki, K., Yamaji, N., Costa, A., Okuma, E., Kobayashi, NI., Kashiwagi, T., Katsuhara, M., Wang, C., Tanoi, K., Murata, Y., Schroeder, JI. Ma, JF., Horie, T.


OsHKT2;2/1-mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress
Journal of Plant Research,129(1):67-77 2016
Author:Suzuki K., Costa A., Nakayama H., Katsuhara M., Shinmyo A., Horie T.


Dynamic regulation of the root hydraulic conductivity of barley plants in response to salinity/osmotic stress.
Plant and Cell Physiology,56(5):875–882 2015
Author:Kaneko T., Horie T., Nakahara Y., Shibasaka M., Katsuhara M.


HKT transporters mediate salt stress resistance in plants: from structure and function to the field.
Current Opinion in Biotechnology ,32:113–120 2015
Author:Hamamoto S., Horie, T., Hauser, F., Deinlein, U., Schroeder, JI., Uozumi, N.


CO2 Transport by PIP2 Aquaporins of Barley.
Plant and Cell Physiology,55(2):251–257 2014
Author:Mori IC., Rhee J., Shibasaka M., Sasano S., Kaneko T., Horie T., Katsuhara M.


Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite.
Plant Biotechnology,31:213–219 2014
Author:Katsuhara M., Sasano S., Horie T., Matsumoto T., Rhee J., Shibasaka M.


Plant salt-tolerance mechanisms.
Trends in Plant Science,19(6):371-379 2014
Author:Deinlein, U., Stephan, AB., Horie, T., Luo, W., Xu, G., Schroeder, JI.


Using membrane transporters to improve crops for sustainable food production
Nature,497:60-66 2013
Author:Schroeder, JI., Delhaize, E., Frommer, WB., Guerinot, ML., Harrison, MJ., Herrera-Estrella, L., Horie, T., Kochian, LV., Munns, R., Nishizawa, NK., Tsay, YF., Sanders D.


Salinity Tolerance Mechanisms in Glycophytes: An Overview with the Central Focus on Rice Plants
Rice,5(11) 2012
Author:Horie, T., Karahara, I., Katsuhara, M.


Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana
Journal of Plant Research,125(1):147-153 2012
Author:Hooijmaijers, C., Rhee, JY., Kwak, KJ., Chung, GC et al.


AtHKT1;1 mediates Nernstian sodium channel transport properties in Arabidopsis root stelar cells
PLoS ONE,6:e24725 2011
Author:Xue, S., Yao, X., Luo, W., Jha, D., Tester, M., Horie, T., Schroeder, JI.


K+ transport by the OsHKT2;4 transporter from rice with atypical Na+ transport properties and competition in permeation of K+ over Mg2+ and Ca2+ ions
Plant Physiology,156(3):1493-1507 2011
Author:Horie, T., Brodsky, DE., Costa, A., Kaneko, T., Schiavo, FL., Katsuhara, M., Schroeder, JI.


Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt-tolerance in tobacco BY2 cells
Journal of Bioscience and Bioengineering,111(3):346-356 2011
Author:Horie, T., Sugawara, M., Okada, T., Taira, K. et al.


Mechanisms of Water Transport Mediated by PIP Aquaporins and their Regulation via Phosphorylation Events under Salinity Stress in Barley Roots
Plant&Cell Physiology,52(4):663-675 2011
Author:Horie, T., Kaneko, T., Sugimoto, G., Sasano, S. et al.


Differential sodium and potassium transport selectivitiesof the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells
Plant Physiology,152(1):341-355 2010
Author:Yao, X., Horie, T., Xue, S., Leung, HY. et al (Horie, T.: Co-first author).


A conserved primary salt tolerance mechanism mediated by HKT transporters: a mechanism for sodium exclusion and maintenance of high K+/Na+ ratio in leaves during salinity stress
Plant, Cell&Environment,33:552-565 2010
Author:Hauser, F. and Horie, T.


HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants
Trends in Plant Science,14(12):660-668 2009
Author:Horie, T., Hauser, F. and Schroeder, JI.


Functions of HKT transporters in sodium transport in roots and in protecting leaves from salinity stress
Plant Biotechnology,25(3):233-239 2008
Author:Horie, T., Sugawara, M., Okunou, K., Nakayama, H. et al.


Drought Stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum
Plant&Cell Physiology,49(5):801-813 2008
Author:Mahdieh, M., Mostajeran, NA., Horie, T. and Katsuhara, M.


Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved roots for growth
The EMBO Journal,26(12):3003-3014 2007
Author:Horie, T., Costa, A., Kim, TH., Han, MJ. et al.


Nomenclature for HKT transporters, key determinants of plant salinity tolerance
Trends in Plant Science,11(8):372-374 2006
Author:Platten, D., Cotsaftis, O., Berthomieu, P., Bohnert, H. et al.


Calcium regulation of sodium hypersensitivities of sos3 and athkt1 mutants
Plant&Cell Physiology,47(5):622-633 2006
Author:Horie, T., Horie, R., Chan, WY., Leung, HY. et al.


Improving salt tolerance in plant cells
Plant Biotechnology,22(5):477-487 2005
Author:Nakayama, H., Horie, T., Yonamine, I., Shinmyo, A. et al.


Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na+ unloading from xylem vessels to xylem parenchyma cells
The Plant Journal,44(6):928-938 2005
Author:Sunarpi., Horie, T., Motoda, J., Kubo, M. et al (Horie, T.: Co-first author).


Sodium transporters in plants. Diverse genes and physiological functions
Plant Physiology,136(1):2457-2462 2004
Author:Horie, T. and Schroeder, JI.


Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana
Proceedings of the National Academy of Sciences of the United States of America,101(43):15404-15409 2004
Author:Gong, JM., Waner, DA., Horie, T., Li, SL. et al.


耐塩性植物の分子育種における陽イオン輸送分子の利用
ファインケミカル,31(7):5-16 2002
Author:吉田和哉、仲山英樹、堀江智明、新名惇彦


Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants
Proceedings of the National Academy of Sciences of the United States of America,99(9):6428-6433 2002
Author:Mäser, P., Hosoo, Y., Goshima, S., Horie, T. et al.


Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1
FEBS Letter,531(2):157-161 2002
Author:Mäser, P., Eckelman, B., Vaidyanathan R., Horie, T. et al.


Two types of HKT transporters with different properties of Na+ and K+ transport in Oryza sativa
The Plant Journal,27(2):129-138 2001
Author:Horie, T., Yoshida, K., Nakayama, H., Yamada, K. et al.


講演・口頭発表等
野生イネ Oryza rufipogon と栽培イネ Oryza sativa の交雑系統群を利用した新規 Na+ 輸送系遺伝子・ 変異の探索
日本育種学会 2017(Oct.)
Presenter:堀内智貴、石川亮、田野井慶太朗、小林奈通子、石井尊生、堀江智明


シロイヌナズナのathkt1欠損株におけるNa, K循環と蓄積の解析
日本植物生理学会 2017(Mar.)
Presenter:竹林昂亮,菊永英寿,加藤恵,横山隆亮,西谷和彦,渡部浩司,須田利美,黒森崇,石川敦司,堀江智明,鈴井伸郎,尹永根,河地有木,藤巻秀,樋口恭子,森泉,浜本晋,魚住信之


OsHKT1 transporters-mediated Na+ exclusion in vasculatures contributes to salt tolerance of rice
(International Workshop on) Strategies of plants and animals on environmental response/adaptation 2017(Mar.)
Presenter:Horie, T.


Mechanisms of Na+ exclusion from leaves in rice upon salinity stress
International Workshop on Soil-Microbe-Plant Interaction 2016(Dec.)
Presenter:Horie, T.


Mechanisms of Na+ homeostasis in rice upon salinity stress and attempts toward breeding of salt tolerant rice cultivars
DEMETERRES Workshop 2015(Oct.)
Presenter:Horie, T.


シロイヌナズナNa輸送体AtHKT1の花器官の形成への関与
日本植物生理学会 2015(Mar.)
Presenter:浜本晋、平林一輝、加藤恵,堀江智明、Schroeder, JI, Blumwald E., 横山隆亮、西谷和彦、石川敦司,魚住信之


過酸化水素輸送性アクアポリンの同定と機能/発現解析
日本植物学会 2014(Sep.)
Presenter:且原真木、篠野静香、堀江智明、Jiye Ree、柴坂三根夫


塩類集積土壌における 植物のナトリウム毒性回避機構
日本育種学会 2014(Sep.)
Presenter:堀江智明


イネ(Oryza sativa)のOsHKT1;5 Na+輸送体遺伝子破壊株の性格付け
日本植物生理学会 2013(Mar.)
Presenter:鈴木圭、柏木達彦、山地直樹、Gynheung An、馬 建鋒、堀江智明


Root hydraulic conductivity and PIP aquaporins of barely and rice in response to salinity/osmotic stress
International Workshop on Plant Membrane Biology 2013
Presenter:Katsuhara, M., Kaneko, T., Horie, T., Tsuji, N., Shibasaka, M.


OsHKT1;5-mediated Na+ transport controls a high K/Na ratio of young leaf blades in rice under salinity stress
International Workshop on Plant Membrane Biology 2013
Presenter:Horie, T., Suzuki, K., Kashiwagi, T., Yamaji, N., An, G., Ma, JF.


Mechanisms of ion and water homeostasis in response to salinity stress
Gordon Research Conference on SALT & WATER STRESS IN PLANTS 2012
Presenter:Horie, T.


Ion and Water Homeostasis during Salinity Stress in Plants
Keystone Symposia’s Meeting on: Plant Abiotic Stress Tolerance Mechanisms,Water and Global Agriculture 2011(Jan.)
Presenter:Horie, T


Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress
International Symposium: Strategies of Plants against Global Environmental Change 35 2011
Presenter:Kaneko, T., Horie, T., Sugimoto, G., Shibasaka, M., Katsuhara, M.


HKT1-mediated Na+ reabsorption from xylem in the mechanism of plant salt tolerance
International Symposium: Strategies of Plants against Global Environmental Change 59 2011
Presenter:Horie, T., Xue, S., Uozumi, N., Schroeder, JI.


HKT transporter-mediated K+ homeostasis in Oryza sativa plants
3rd Japan-China Joint Workshop on Plant Nutrition 2011
Presenter:Horie, T. Brodsky, DE., Costa, A., Kaneko, T., Katsuhara, M., Schroeder, JI.


高浸透圧ストレス下におけるオオムギ根の水輸送活性制御機構の解明
日本植物生理学会年会熊本大会 2010(Mar.)
Presenter:金子智之、堀江智明、柴坂三根夫、且原真木


オオムギPIP1型と2型アクアポリンのへテロマー形成の分子機構
日本植物生理学会年会熊本大会 2010(Mar.)
Presenter:堀江智明、且原 真木


Sodium and potassium transport selectivities of the OsHKT2;1 and OsHKT2;2 transporters in plant cells
Plant Biology 2009 American Society of Plant Biologists 2009(Jul.)
Presenter:Yao, X., Horie, T., Leung, HY., Brodsky, DE., Schroeder, JI.


原形質膜型アクアポリンPIP1とPIP2の共発現による活性化メカニズムの解析
日本植物生理学会年会名古屋大会 2009(Mar.)
Presenter:柴坂三根夫、堀江智明、且原真木


オオムギ根の水輸送活性は塩ストレス下において翻訳後修飾によって抑制されている
日本植物生理学会年会名古屋大会 2009(Mar.)
Presenter:堀江智明、且原真木


Transcriptional Regulations and Water Transport Activity of PIP-type Aquaporins in Roots of Barley
Gordon Research Conference on SALT&WATER STRESS IN PLANTS 2008(Sep.)
Presenter:Horie, T., Sugimoto, G., Kaneko, T., Shibasaka, M., Katsuhara, M.


Physiological roles of HKT-type transporters/channels in salinity tolerance and growth in plants
Gordon Research Conference on SALT&WATER STRESS IN PLANTS 2008(Sep.)
Presenter:Horie, T.


オオムギ原形質膜型アクアポリンの発現制御と水輸送特性
日本植物生理学会年会札幌大会 2008(Mar.)
Presenter:堀江智明、杉本元気、柴坂三根夫、且原真木


The Arabidopsis sodium transporter, AtHKT1, mediates sodium unloading from xylem vessels: An essential function for protecting leaves from salinity stress
Keystone Symposia 2006 2006(Apr.)
Presenter:Horie, T., Sunarpi, Uozumi, N., Schroeder, JI.


The Arabidopsis sodium transporter, AtHKT1, is an essential factor for salt tolerance and for reducing sodium accumulation in leaves
Plant Biology 2004 American Society of Plant Biologists 2004(Jul.)
Presenter:Horie, T., Mäser, P, Eckelman, B., Uozumi, N., Schroeder, JI.


The Arabidopsis sodium transporter, AtHKT1, is an essential factor for salt tolerance and for reducing sodium accumulation in leaves
Keystone Symposia 2004 2004(Feb.)
Presenter:Horie, T., Mäser, P, Eckelman, B., Uozumi, N., Schroeder, JI.


The Arabidopsis sodium transporter enhances salt tolerance by reducing sodium accumulation in leaves
22nd Symposium in Plant Biology: Signals and Pathways, Systems-Based Approaches 2003(Jan.)
Presenter:Horie, T., Mäser, P, Eckelman, B., Uozumi, N., Schroeder, JI.


Potassium-Channel-like Selectivity Filters Determine Potassium Selectivity in HKT Transporters from Plants
Plant Biology 2002 American Society of Plant Biologists 2002(Jul.)
Presenter:Mäser, P., Horie, T., Eckelman, B., Uozumi, N., Schroeder, JI.


イネ (Oryza sativa) における2種のHKTタンパク質は異なるK+, Na+ 輸送能を有する
日本植物生理学会年会福岡大会 2001(Mar.)
Presenter:堀江智明、吉田和哉、仲山英樹、山田克幸、老木成稔、新名惇彦


イネの高親和性K+-Na+ 共輸送体をコードするHKT遺伝子の発現と機能解析
日本生物工学会年会札幌大会 2000(Aug.)
Presenter:堀江智明、仲山英樹、吉田和哉、新名惇彦


The Rice Potassium Uptake Transporter
The 6th International Congress of Plant Molecular Biology 2000(Jun.)
Presenter:Horie, T., Nakayama, H., Yoshida, K., Shinmyo, A.


イネHKT遺伝子の根におけるK+, Na+よる転写制御機構
日本植物生理学会年会仙台大会 1999(Mar.)
Presenter:堀江智明、仲山英樹、吉田和哉、新名惇彦


イネの高親和性K+輸送体をコードするHKT1遺伝子の単離
日本分子生物学会年会京都大会 1997(Dec.)
Presenter:堀江智明、仲山英樹、吉田和哉、新名惇彦