HORIE Tomoaki

Academic Assembly School of Science and Technology Institute of Textile Science and Technology

Faculty of Textile Science and Technology Division of Applied Biology 


Research Keyword

    Salt tolerance, Rice, Barley, Arabidopsis, Na+/K+ transport, Water transport, Ion homeostasis

Field Of Study

  • Plant Molecular Biology
  • Plant Physiology
  • Plant Cellular Engineering



Mail Address



  • The HKT1 Na+ transporter protects plant fertility by decreasing Na+ content in stamen filaments
    Takeshi Uchiyama, Shunya Saito, Taro Yamanashi, Megumi Kato, Kosuke Takebayashi, Shin Hamamoto, Masaru Tsujii, Tomoko Takagi, Noriko Nagata, Hayato Ikeda, Hidetoshi Kikunaga, Toshimi Suda, Sho Toyama, Misako Miwa, Shigeo Matsuyama, Mitsunori Seo, Tomoaki Horie, Takashi Kuromori, Mutsumi Yamagami, Yasuhiro Ishimaru, Nobuyuki Uozumi
    Science Advances, 9(22), eadg5495, 02 Jun. 2023
  • Distinct Functions of the Atypical Terminal Hydrophilic Domain of the HKT Transporter in the Liverwort Marchantia polymorpha
    Imran S, Oyama M, Horie R, Kobayashi NI, Costa A, Kumano R, Hirata C, Tran STH, Katsuhara M, Tanoi K, Kohchi T, Ishizaki K, Horie T.
    Plant and Cell Physiology, 63(6), 802-816, Apr. 2022
  • Mechanisms Activating Latent Functions of PIP Aquaporin Water Channels via the Interaction between PIP1 and PIP2 Proteins.
    Shibasaka M, Horie T, Katsuhara M.
    Plant and Cell Physiology, 62(1), 92-99, 25 Mar. 2021, Refereed電子ジャーナル
  • Functions and structure of roots and their contributions to salinity tolerance in plants.
    Karahara I, Horie T
    BREEDING SCIENCE, 71(1), 89-108, 08 Mar. 2021, Refereed電子ジャーナル
  • A survey of barley PIP aquaporin ionic conductance reveals Ca2+-sensitive HvPIP2;8 Na+ and K+ conductance.
    Tran STH, Horie T, Imran S, Qiu J, McGaughey S, Byrt CS, Tyerman SD, Katsuhara M.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(19), 7135, 27 Sep. 2020, Refereed電子ジャーナル
  • 営農型太陽光発電のパネル制御が水稲栽培に及ぼす影響
    農業食料工学会誌, 82(4), 380-389, 01 Jul. 2020, Refereed
  • Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots.
    Al Nayef M, Solis C, Shabala L, Ogura T, Chen Z, Bose J, Maathuis FJM, Venkataraman G, Tanoi K, Yu M, Zhou M, Horie T, Shabala S.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 21(14), 4882, Jul. 2020, Refereed電子ジャーナル
  • Doing 'business as usual' comes with a cost: evaluating energy cost of maintaining plant intracellular K+ homeostasis under saline conditions
    Rubio F, Nieves-Cordones M, Horie T, Shabala S.
    New Phytologist, 225(3), 1097-1104, Feb. 2020WebofScience電子ジャーナル
  • Expression and Ion Transport Activity of Rice OsHKT1;1 Variants
    Imran S, Horie T, Katsuhara M.
    PLANTS-BASEL, 9(1), Jan. 2020, RefereedWebofScience電子ジャーナル
  • High-Affinity K+ Transporters from a Halophyte, Sporobolus virginicus, Mediate Both K+ and Na+ Transport in Transgenic Arabidopsis, X. laevis Oocytes and Yeast
    Tada Y, Endo C, Katsuhara M, Horie T, Shibasaka M, Nakahara Y, Kurusu T.
    Plant and Cell Physiology, 60(1), 176-187, Jan. 2019, RefereedWebofScience電子ジャーナル
  • Energy costs of salinity tolerance in crop plants
    Tyerman SD Munns R FrickeW Arsova B; Barkla B; Bose J; Bramley H; Byrt C; Chen Z; Colmer TD; Cuin T; Day DA; Foster KJ; Gilliham M; Henderson SW; Horie T; Jenkins CLD; Kaiser BN; Katsuhara M; Plett D; Miklavcic SJ; Roy SJ; Rubio F; Shabala S; Shelden M; Soole K; Taylor NL; Tester M; Watt M; Wege S; Wegner LH; Wen Z
    New Phytologist, 221(1), 25-29, Jan. 2019WebofScience電子ジャーナル
  • T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress
    Oda, Y., Kobayashi, NI., Tanoi, K., Ma, JF., Itou, Y., Katsuhara, M., Itou, T., Horie, T.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19:235, 2018WebofScienceリポジトリ電子ジャーナル
  • Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport
    Rhee,J., Horie, T., Shizuka, S., Nakahara, Y., Maki, K.
    Physiologia Plantarum, 159(1), 120–128, 2017
  • OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice
    Kobayashi, NI., Yamaji, N., Yamamoto, H., Okubo, K., Ueno, H., Costa, A., Tanoi, K., Matsumura, H., Fujii-Kashino, M., Horiuchi, T., Nayef, MA., Shabala, S., An, G., Ma, JF., Horie, T.
    The Plant Journal, 91, 657-670, 2017
  • A magnesium transporter OsMGT1 plays a critical role in salt tolerance in rice
    Chen, ZC., Yamaji, N., Horie, T., Che, J., Li, J., An, G., Ma, JF.
    Plant Physiology, 174, 1837-1849, 2017
  • Genomics, physiology, and molecular breeding approaches for improving salt tolerance
    Ismail, AM., Horie, T.
    Annual Review of Plant Biology, 68, 405-434, 2017
  • OsHKT2;2/1-mediated Na+ influx over K+ uptake in roots potentially increases toxic Na+ accumulation in a salt-tolerant landrace of rice Nona Bokra upon salinity stress
    Suzuki K., Costa A., Nakayama H., Katsuhara M., Shinmyo A., Horie T.
    Journal of Plant Research, 129(1), 67-77, 2016
  • OsHKT1;4-mediated Na+ transport in stems contributes to Na+ exclusion from leaf blades of rice at the reproductive growth stage upon salt stress
    Suzuki, K., Yamaji, N., Costa, A., Okuma, E., Kobayashi, NI., Kashiwagi, T., Katsuhara, M., Wang, C., Tanoi, K., Murata, Y., Schroeder, JI. Ma, JF., Horie, T.
    BMC Plant Biology, 16:22, 2016電子ジャーナル
  • Dynamic regulation of the root hydraulic conductivity of barley plants in response to salinity/osmotic stress.
    Kaneko T., Horie T., Nakahara Y., Shibasaka M., Katsuhara M.
    Plant and Cell Physiology, 56(5), 875–882, 2015
  • HKT transporters mediate salt stress resistance in plants: from structure and function to the field.
    Hamamoto S., Horie, T., Hauser, F., Deinlein, U., Schroeder, JI., Uozumi, N.
    Current Opinion in Biotechnology, 32, 113–120, 2015
  • Plant salt-tolerance mechanisms.
    Deinlein, U., Stephan, AB., Horie, T., Luo, W., Xu, G., Schroeder, JI.
    Trends in Plant Science, 19(6), 371-379, 2014
  • CO2 Transport by PIP2 Aquaporins of Barley.
    Mori IC., Rhee J., Shibasaka M., Sasano S., Kaneko T., Horie T., Katsuhara M.
    Plant and Cell Physiology, 55(2), 251–257, 2014
  • Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite.
    Katsuhara M., Sasano S., Horie T., Matsumoto T., Rhee J., Shibasaka M.
    Plant Biotechnology, 31, 213–219, 2014
  • Using membrane transporters to improve crops for sustainable food production
    Schroeder, JI., Delhaize, E., Frommer, WB., Guerinot, ML., Harrison, MJ., Herrera-Estrella, L., Horie, T., Kochian, LV., Munns, R., Nishizawa, NK., Tsay, YF., Sanders D.
    Nature, 497, 60-66, 2013
  • Hydrogen peroxide permeability of plasma membrane aquaporins of Arabidopsis thaliana
    Hooijmaijers, C., Rhee, JY., Kwak, KJ., Chung, GC et al.
    Journal of Plant Research, 125(1), 147-153, 2012, Refereed電子ジャーナル
  • Salinity Tolerance Mechanisms in Glycophytes: An Overview with the Central Focus on Rice Plants
    Horie, T., Karahara, I., Katsuhara, M.
    Rice, 5(11), 2012WebofScienceリポジトリ電子ジャーナル
  • Mechanisms of Water Transport Mediated by PIP Aquaporins and their Regulation via Phosphorylation Events under Salinity Stress in Barley Roots
    Horie, T., Kaneko, T., Sugimoto, G., Sasano, S. et al.
    Plant&Cell Physiology, 52(4), 663-675, 2011, Refereed
  • Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt-tolerance in tobacco BY2 cells
    Horie, T., Sugawara, M., Okada, T., Taira, K. et al.
    Journal of Bioscience and Bioengineering, 111(3), 346-356, 2011, Refereed
  • K+ transport by the OsHKT2;4 transporter from rice with atypical Na+ transport properties and competition in permeation of K+ over Mg2+ and Ca2+ ions
    Horie, T., Brodsky, DE., Costa, A., Kaneko, T., Schiavo, FL., Katsuhara, M., Schroeder, JI.
    Plant Physiology, 156(3), 1493-1507, 2011
  • AtHKT1;1 mediates Nernstian sodium channel transport properties in Arabidopsis root stelar cells
    Xue, S., Yao, X., Luo, W., Jha, D., Tester, M., Horie, T., Schroeder, JI.
    PLOS ONE, 6, e24725, 2011
  • Differential sodium and potassium transport selectivitiesof the rice OsHKT2;1 and OsHKT2;2 transporters in plant cells
    Yao, X., Horie, T., Xue, S., Leung, HY. et al (Horie, T.: Co-first author).
    Plant Physiology, 152(1), 341-355, 2010, Refereed
  • HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plants
    Horie, T., Hauser, F. and Schroeder, JI.
    Trends in Plant Science, 14(12), 660-668, 2009, Refereed
  • Drought Stress alters water relations and expression of PIP-type aquaporin genes in Nicotiana tabacum
    Mahdieh, M., Mostajeran, NA., Horie, T. and Katsuhara, M.
    Plant&Cell Physiology, 49(5), 801-813, 2008, Refereed
  • Functions of HKT transporters in sodium transport in roots and in protecting leaves from salinity stress
    Horie, T., Sugawara, M., Okunou, K., Nakayama, H. et al.
    Plant Biotechnology, 25(3), 233-239, 2008, Refereed
  • Rice OsHKT2;1 transporter mediates large Na+ influx component into K+-starved roots for growth
    Horie, T., Costa, A., Kim, TH., Han, MJ. et al.
    The EMBO Journal, 26(12), 3003-3014, 2007, Refereed
  • Calcium regulation of sodium hypersensitivities of sos3 and athkt1 mutants
    Horie, T., Horie, R., Chan, WY., Leung, HY. et al.
    Plant&Cell Physiology, 47(5), 622-633, 2006, Refereed
  • Nomenclature for HKT transporters, key determinants of plant salinity tolerance
    Platten, D., Cotsaftis, O., Berthomieu, P., Bohnert, H. et al.
    Trends in Plant Science, 11(8), 372-374, 2006, Refereed
  • Improving salt tolerance in plant cells
    Nakayama, H., Horie, T., Yonamine, I., Shinmyo, A. et al.
    Plant Biotechnology, 22(5), 477-487, 2005, Refereed
  • Enhanced salt tolerance mediated by AtHKT1 transporter-induced Na+ unloading from xylem vessels to xylem parenchyma cells
    Sunarpi., Horie, T., Motoda, J., Kubo, M. et al (Horie, T.: Co-first author).
    The Plant Journal, 44(6), 928-938, 2005, Refereed
  • Microarray-based rapid cloning of an ion accumulation deletion mutant in Arabidopsis thaliana
    Gong, JM., Waner, DA., Horie, T., Li, SL. et al.
    Proceedings of the National Academy of Sciences of the United States of America, 101(43), 15404-15409, 2004, Refereed
  • Sodium transporters in plants. Diverse genes and physiological functions
    Horie, T. and Schroeder, JI.
    Plant Physiology, 136(1), 2457-2462, 2004, Refereed
  • Altered shoot/root Na+ distribution and bifurcating salt sensitivity in Arabidopsis by genetic disruption of the Na+ transporter AtHKT1
    Mäser, P., Eckelman, B., Vaidyanathan R., Horie, T. et al.
    FEBS Letter, 531(2), 157-161, 2002, Refereed
  • Glycine residues in potassium channel-like selectivity filters determine potassium selectivity in four-loop-per-subunit HKT transporters from plants
    Mäser, P., Hosoo, Y., Goshima, S., Horie, T. et al.
    Proceedings of the National Academy of Sciences of the United States of America, 99(9), 6428-6433, 2002, Refereed
  • Two types of HKT transporters with different properties of Na+ and K+ transport in Oryza sativa
    Horie, T., Yoshida, K., Nakayama, H., Yamada, K. et al.
    The Plant Journal, 27(2), 129-138, 2001, Refereed

Books and other publications

  • Improving Crop Resistance to Abiotic Stress
    Yamaguchi, T., Uozumi, N. and Horie, T., Chapter 23: Potassium and sodium transporters: improving salinity tolerance in plants
    Wiley-VCH Verlag GmbH & Co., Germany, 523-542 2012

Lectures, oral presentations, etc.

  • Physiological traits study of mutated Ca2+/H+ exchanger, OsCAX1a in BRRIdhan-47
    Pronabananda DAS, Tomoaki HORIE, A.N.K. Mamun, Yoshihiro HASE
    10th International Plant Tissue Culture & Biotechnology Conference, Mar. 2023
  • Mechanisms of Na+ homeostasis in rice upon salinity stress and attempts toward breeding of salt tolerant rice cultivars
    Horie, T.
    DEMETERRES Workshop, Oct. 2015
  • OsHKT1;5-mediated Na+ transport controls a high K/Na ratio of young leaf blades in rice under salinity stress
    Horie, T., Suzuki, K., Kashiwagi, T., Yamaji, N., An, G., Ma, JF.
    International Workshop on Plant Membrane Biology, 2013
  • Root hydraulic conductivity and PIP aquaporins of barely and rice in response to salinity/osmotic stress
    Katsuhara, M., Kaneko, T., Horie, T., Tsuji, N., Shibasaka, M.
    International Workshop on Plant Membrane Biology, 2013
  • Mechanisms of ion and water homeostasis in response to salinity stress
    Horie, T.
    Gordon Research Conference on SALT & WATER STRESS IN PLANTS, 2012, Invited
  • HKT transporter-mediated K+ homeostasis in Oryza sativa plants
    Horie, T. Brodsky, DE., Costa, A., Kaneko, T., Katsuhara, M., Schroeder, JI.
    3rd Japan-China Joint Workshop on Plant Nutrition, 2011, Invited
  • HKT1-mediated Na+ reabsorption from xylem in the mechanism of plant salt tolerance
    Horie, T., Xue, S., Uozumi, N., Schroeder, JI.
    International Symposium: Strategies of Plants against Global Environmental Change 59, 2011
  • Dynamic regulation of root hydraulic conductivity and aquaporins under salt stress
    Kaneko, T., Horie, T., Sugimoto, G., Shibasaka, M., Katsuhara, M.
    International Symposium: Strategies of Plants against Global Environmental Change 35, 2011

Research Themes

  • Elucidation of salinity tolerance mechianisms in plants
  • Elucidation of physiological roles of rice HKTs and development of the technology for breeding of salt tolerant rice cultivars
  • Isolation of genes related to salt stress tolerance from wild and salt tolerant rice