Ph.D. in Cardiovascular Science, University of Tokyo

Cardiology, Cardiovascular Medicine

Metabolism and endocrinology, Endocrinology

2004
Professor, Department of Cardiovascular Research, Shinshu University, School of Medicine

2002
Assistant Professor, Department of Cardiovascular Medicine, University of Tokyo

1994 - 1998, The University of Tokyo, Graduate School of Medicine

1985 - 1991, The University of Tokyo, Faculty of Medicine

Role of adrenomedullin-2/intermedin in the pathogenesis of neovascular age-related macular degeneration.
Kakihara S, Matsuda Y, Hirabayashi H, Imai A, Iesato Y, Sakurai T, Kamiyoshi A, Tanaka M, Ichikawa-Shindo Y, Kawate H, Zhao Y, Zhang Y, Guo Q, Li P, Onishi N, Murata T, Shindo T.
Lab Invest., 103(4), 100038, 2023

Receptor activity-modifying proteins of adrenomedullin (RAMP2/3): Roles in the pathogenesis of ARDS.
Kasahara T, Tanaka M, Zhao Y, Kamiyoshi A, Saurai T, Ichikawa-Shindo Y, Kawate H, Matsuda Y, Zhang Y, Guo Q, Li P, Hoshiyama K, Li J, Onishi N, Hayashi M, Sanjo H, Ishida K, Tanaka S, Kawanata M, ShindoT.
PEPTIDES, 171, 171118, 2023, Refereed

Calcitonin gene-related peptide is not involved in neuropathic pain induced by partial sciatic nerve ligation in mice.
Ishida K, Tanaka S, Shen D, Matsui S, Fuseya S, Shindo, Kawamata M.
Neurosci Lett., 778, 136615, 2022

Studies on safety and efficacy of particles containing a mixture of hydroxyapatite–argentum–titanium oxide (HAT) and sheets coated with HAT particles to be used in masks to improve nasal allergy: I. In vitro studies.
Okazaki N, Yamaki D, Takei T, Shimizu M, Kamatani N, Shindo T.
The Allergy in Practice, 42(3), 37-46, 2022

Studies on safety and efficacy of particles containing a mixture of hydroxyapatite-argentum-titanium oxide (HAT) and sheets coated with HAT particles to be used in masks to improve nasal allergy: II. Cellular, in vivo, and clinical studies.
Okazaki N, Yamaki D, Takei T, Shimizu M, Kamatani N, Shindo T.
Eur Arch Otorhinolaryngol., 279(9), 4425-4433, 2022

Receptor activity modifying protein RAMP sub-isoforms and their functional differentiation, which regulates functional diversity of adrenomedullin.
Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Sakurai T.
Biology., 11(5), 788, 2022

An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility.
Negishi K, Aizawa K, Shindo T, Suzuki T, Sakurai T, Saito Y, Miyakawa T, Tanokura M, Kataoka Y, Maeda M, Tomida S, Morita M, Kario K, Nagai R, Imai Y.
Sci Rep., 12(1), 8844, 2022

Mid-regional pro-adrenomedullin is a novel biomarker for arterial stiffness as the criterion for vascular failure in a cross-sectional study.
Koyama T, Kuriyama N, Suzuki Y, Saito S, Tanaka R, Iwao M, Tanaka M, Maki T, Itoh H, Ihara M, Shindo T, Uehara R.
Sci Rep., 11(1), 305, 2021

Adrenomedullin-RAMP2 system ameliorates subretinal fibrosis by suppressing epithelial-mesenchymal transition in age-related macular degeneration.
Tanaka M, Kakihara S, Hirabayashi K. Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Cui N, Wei Y, Zhao Y, Aruga K, Yamauchi A, Murata M, Shindo T.
Am J Pathol., 191(4), 652-668, 2021

Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing.
Sakurai T, Shindo T.
STAR Protoc., 2(2), 100509, 2021

Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress.
Cui N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Tanaka M, Wei Y, Kakihara S, Zhao Y, Aruga K, Kawagishi H, Nakada T, Yamada M, Shindo T.
Endocrinology., 162(3), 1-20, 2021

Adrenomedullin-RAMP2 system ameliorates pulmonary fibrosis by regulating TGF-β-Smads pathway and differentiation of myofibroblasts.
Wei Y, Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Cui1 N, Kakihara S, Zhao Y, Aruga K, Sanjo H, Shindo T.
Endocrinology., 162(8), 1-22, 2021

Production of genetically engineered mice with higher efficiency, lower mosaicism, and mulitiplexing capability using maternally expressed Cas9.
Sakurai T, Kamiyoshi A, Kawate H, Watanabe S, Sato M, Shindo T.
Sci Rep., 10(1):1091,, 2020

Placental extract ameliorates liver fibrosis in a methionine- and choline-deficient diet-induced mouse model of non-alcoholic steatohepatitis.
Yamauchi A. Tone T, Tokedo A, Igarashi K, Sugimoto K, Miyai H, Deng D, Nakamura J, Lim HS, Kaku T, Hirano E, Shindo T.
Biomedical Research., 41(1), 1–12, 2020

Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts.
Dai K, Tanaka M, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Cui N, Wei Y, Tanaka M, Kakihara S, Matsui S, Shindo T.
Oncogene., 39(9), 1914-1930, 2020

Investigation of the therapeutic mechanism of subthreshold micropulse laser irradiation in retina.
Hirabayashi K, Kakihara S, Tanaka M, Shindo T, Murata T.
Graefes Arch Clin Exp Ophthalmol., 285(5), 1039-1047, 2020

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase
Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M , Shindo T.
Sci Rep, 9:9923., 2019

Regulation of cardiovascular development and homeostasis by the adrenomedullin-RAMP system
Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Sakurai T
Peptides., 111, 55-61, 2019

Development of a mouse iron overload-induced liver injury model and evaluation of the beneficial effects of placenta extract on iron metabolism
Yamauchi A. Kamiyoshi A, Sakurai T, Miyazaki H, Hirano E, Lim HS, Kaku T, Shindo T. Development of a mouse iron overload-induced liver injury model and evaluation of the beneficial effects of placenta extract on iron metabolism
Heliyon., 10;5(5):e01637, 2019

Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice
Yamauchi A, Kamiyoshi A, Sakurai T, Miyazaki H, Hirano E, Lim HS, Kaku T, Shindo T.
Heliyon., 5(10):e02655., 2019

Development of a novel model of central retinal vascular occlusion and therapeutic potential of adrenomedullin-RAMP2 system
Hirabayashi K, Tanaka M, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Dai K, Cui N, Wei Y, Nakamura K, Iida S, Matsui S, Yamauchi A, Murata T, Shindo T
Am J Pathol., 189(2), 449-466, 2019

Endogenous Calcitonin Gene-Related Peptide Deficiency Exacerbates Postoperative Lymphedema by Suppressing Lymphatic Capillary Formation and M2 Macrophage Accumulation.
Matsui S, Tanaka M, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Dai K, Cui N, Wei Y, Tanaka M, Kakihara S, Nakamura K, Yamauchi A, Ishida K, Tanaka S, Kawamata M, Shindo T.
Am J Pathol., 189(12), 2487-2502, 2019

Porcine placental extract facilitates memory and learning in aged mice.
Yamauchi A, Tone T, Sugimoto K, Lim HS, Kaku T, Tohda C, Shindo T, Tamada K, Mizukami Y, Hirano E.
Food Sci Nutr., (7), 2995–3005, 2019

RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders
Liu T, Kamiyoshi A, Tanaka M, Iida S, Sakurai T, Ichikawa-Shindo Y, Kawate H, Hirabayashi K, Dai K, Cui N, Tanaka M, Wei Y, Nakamura K, Matsui S, Yamauchi A, Shindo T
Peptides., 110, 10-18, 2018

Endogenous calcitonin gene-related peptide suppresses ischemic brain injuries and progression of cognitive decline
Zhai L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Xian X, Hirabayashi K, Dai K, Cui N, Tanimura K, Liu T, Wei Y, Tanaka M, Tomiyama H, Yamauchi A,Igarashi K, Shindo T
J Hypertens., 36(4), 876-891, 2018

Endogenous Calcitonin Gene-Related Peptide Regulates Lipid Metabolism and Energy Homeostasis in Male Mice.
Liu T, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Yang L, Tanaka M, Xian X, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
Endocrinology., 158(5), 1194-1206, 2017

Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
Endocrinology., 158(5), 1359−1372, 2017

Adrenomedullin suppresses vascular endothelial growth factor-induced vascular hyperpermeability and Inflammation in retinopath
Imai A, Toriyama Y, Iesato Y, Hirabayashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Liu T, Xian X, Zhai L, Dai K, Tanimura K, Liu T, Cui N, Yamauchi A, Murata T, Shindo T"
Am J Phthol., 187(5), 999-1015, 2017

Placental extract ameliorates non-alcoholic steatohepatitis (NASH) by exerting protective effects on endothelial cells
Yamauchi A, Kamiyoshi A, Koyama T, Iinuma N, Yamaguchi S, Miyazaki H, Hirano E, Kaku T, Shindo T
Heliyon., 2017

Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques.
Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K.
Sci Rep, 6, 35861, Oct. 2016

The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis.
Tanaka M, Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Liu T, Xian X, Imai A, Zhai L, Hirabayashi K, Owa S, Yamauchi A, Igarashi K, Taniguchi S, Shindo T.
Cardiovasc Res, 111(4), 398-409, Sep. 2016

Effects of Adrenomedullin on Doxorubicin-Induced Cardiac Damage in Mice.
Yoshizawa T, Takizawa S, Shimada S, Tokudome T, Shindo T, Matsumoto K.
Biol Pharm Bull, 39(5), 737-46, May 2016

Adrenomedullin: A potential therapeutic target for retinochoroidal disease.
Iesato Y, Yuda K, Chong KT, Tan X, Murata T, Shindo T, Yanagi Y.
Prog Retin Eye Res, 52, 112-29, May 2016

A non-inheritable maternal Cas9-based multiple-gene editing system in mice.
Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T.
Sci Rep, 6, 20011, Jan. 2016

Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury.
Ayuzawa N, Nagase M, Ueda K, Nishimoto M, Kawarazaki W, Marumo T, Aiba A, Sakurai T, Shindo T, Fujita T.
Hypertension, 67(1), 99-106, Jan. 2016

Importance of Endogenous Atrial and Brain Natriuretic Peptides in Murine Embryonic Vascular and Organ Development.
Tokudome T, Kishimoto I, Shindo T, Kawakami H, Koyama T, Otani K, Nishimura H, Miyazato M, Kohno M, Nakao K, Kangawa K.
Endocrinology, 157(1), 358-67, Jan. 2016

Pathophysiological Function of Endogenous Calcitonin Gene-Related Peptide in Ocular Vascular Diseases
Toriyama Y, Iesato Y, Imai A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Igarashi K, Tanaka M, Liu T, Xian X, Zhai L, Owa S, Murata T, Shindo T.
Am J Pathol., 185(6), 1783-1794, 2015

Adrenomedullin-RAMP2 System in Vascular Endothelial Cells
Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Shindo T.
J. Atheroscler. Thromb, 22(7), 647-653, 2015

Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia
Igarashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T.
Peptides., 62, 21-31, 2014

Functional differentiation of RAMP2 and RAMP3 in their regulation of vascular system
Yamauchi A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Igarashi K, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T.
J Mol Cell Cardiol., 77, 73-85, 2014

Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T.
PLOS ONE, 9(2), e87667, 2014

Calcitonin Gene-related Peptide Is Involved in Inflammatory Pain but Not in Postoperative Pain.
Ishida K, Kawamata T, Tanaka S, Shindo T, Kawamata M.
Anesthesiology., 121(5), 1068-79, 2014

Pathophysiological roles of adrenomedullin and its receptor activity modifying system.
Shindo T, Sakurai T, Kamiyoshi A, Shindo Y, Kawate H, Koyama T.
Folia Pharmacol. Jpn, 143(5), 232-235, 2014

A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice.
Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T.
BMC Biotechnol., 14(1), 69, 2014

Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury
Yang　L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T.
J Mol Cell Cardiol., 59, 55-66, 2013

Vascular Endothelial Adrenomedullin-RAMP2 System Is Essential for Vascular Integrity and Organ Homeostasis
Koyama T, Ochoa-Callejero L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Arai T, Yoshizawa T, Iesato Y, Lei Y, Uetake R, Okimura A, Yamauchi A, Tanaka M, Igarashi K, Toriyama Y, Kawate H, Adams R.H, Kawakami H, Mochizuki N, Martínez A, Shindo T
Circulation, 127(7), 842-853, 2013

Novel regulation of cardiac ｍetabolism and homeostasis by the adrenomedullin-RAMP2 system
Yoshizawa T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Nakada T, Kashihara T, Yamada M, Kawakami H, Nakanishi H, Taguchi R, Nakanishi T, Akazawa H, Shindo T.
Hypertension, 61(2), 341-351, 2013

Adrenomedullin-RAMP2 System Is Crucially Involved in Retinal Angiogenesis
Iesato Y, Toriyama Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Igarashi K, Murata T, Shindo T
Am J Pathol., 182(6), 2380-2390, 2013

Regulation of adrenomedullin and its family peptide by RAMP system
Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Shimoyama N, Iinuma N, Arai T, Miyagawa S.
Curr Prot Pept Sci., 14(5), 347-367, 2013

Structural basis for extracellular interactions between calcitonin receptor-like receptor and receptor activity-modifying protein 2 for adrenomedullin-specific binding.
Kusano S, Kukimoto-Niino M, Hino N, Ohsawa N, Okuda K, Sakamoto K, Shirouzu M, Shindo T, Yokoyama S.
Protein Sci., 21, 199-210, 2012

Adrenomedullin-RAMP2 system in cardiovascular development and homeostasis
Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y.
Curr Hypertens Rev., 7(4), 217-227, 2011

Induction of LYVE-1/stabilin-2-positive liver sinusoidal endothelial-like cells from embryoid bodies by modulation of adrenomedullin-RAMP2 signaling
Arai T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Iesato Y, Koyama T, Yoshizawa T, Uetake R, Yamauchi A, Yang L, Kawate H, Ogawa S, Kobayashi A, Miyagawa S, Shindo T.
PEPTIDES, 32(9), 1855-1865, 2011

Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver
Iinuma, Nobuyoshi; Sakurai, Takayuki; Kamiyoshi, Akiko; Ichikawa-Shindo, Yuka; Arai, Takuma; Yoshizawa, Takahiro; Koyama, Teruhide; Uetake, Ryuichi; Kawate, Hisaka; Muto, Shin-ichi; Tagawa, Yoh-ichi; Miyagawa, Shinichi; Shindo, Takayuki
PEPTIDES, 31(5), 865-871, May 2010

Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload.
Takeda N, Manabe I, Uchino Y, Eguchi K, Matsumoto S, Nishimura S, Shindo T, Nagai R.　
J Clin Invest., 120(1), 254-65, 2010

Endogenous a-CGRP mitigates liver fibrosis in chronic hepatitis induced by repeated administration of concanavalin A.
Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y. Iinuma N, Kawate H, Yoshizawa T, Koyama T, Muto S, Shindo T.
Liver International., 29(5), 642-9, 2009

Regulation of angiogenesis and vascular integrity by adrenomedullin-RAMP2 system.
Shindo Y, Sakurai T, Kamiyoshi A, Koyma T, Yoshizawa T, Shindo T.　
J Soc Circ Res., 32(2), 33-35, 2009

Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution.
Sakurai T, Kamiyoshi A, Watanabe S, Sato M, Shindo T.
Transgenic Res., 17, 149-55, 2008

GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity.
Ichikawa-Shindo Y, Sakurai T, Kamiyoshi A, Kawate H, Iinuma N, Yoshizawa T, Koyama T, Fukuchi J, Iimuro S, Moriyama N, Kawakami H, Murata T, Kangawa K, Nagai R, Shindo T.　
J Clin Invest., (118), 29-39, 2008

Crystal structure of the human receptor activity-modifying protein 1 extracellular domain
Kusano S, Kukimoto-Niino M, Akasaka R, Toyama M, Terada T, Shirouzu M, ShindoT, Yokoyama S.
Protein Sci., 17(11), 1907-14, 2008

Kruppel-like factor 5 causes cartilage degradation through transactivation of matrix metalloproteinase 9.: Shinoda Y, Ogata N,
Higashikawa A, Manabe I,Shindo T, Yamada T, Kugimiya F, Ikeda T, Kawamura N, Kawasaki Y, Tsushima K, Takeda N, Nagai R, Hoshi K, Nakamura K, Chung UI, Kawaguchi H.
J Biol Chem., 283(36), 24682-9, 2008

Adrenomedullin insufficiency increases allergen-induced airway hyperresponsiveness in mice.
Yamamoto H, Nagase T, Shindo T, Teramoto S, Aoki-Nagase T, Yamaguchi Y, Hanaoka Y, Kurihara H, Ouchi Y
J Appl Physiol., 102(6), 2361-8, 2007

Azelnidipine, a long-acting dihydropyridine calcium channel blocker, suppresses the development of cardiac hypertrophy and fibrosis in adrenomedullin knock out mice.
Shindo, T; Niu, P; Kamiyoshi, A; Sakurai, T; Ichikawa, Y; Iinuma, N; Kawate, H
Circulation, 114(18), 13-13, 2006

The synthetic retinoid Am80 reduces scavenger receptor expression and atherosclerosis in mice by inhibiting IL-6
Takeda, N; Manabe, I; Shindo, T; Iwata, H; Iimuro, S; Kagechika, H; Shudo, K; Nagai, R
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 26(5), 1177-1183, 2006

Endogenous alpha CGRP protects against concanavalin A-induced hepatitis in mice
Kainiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Fukuchi, J; Kawate, H; Muto, S; Tagawa, Y; Shindo, T
Biochemical and biophysical research communications, 343(1), 152-158, 2006

Significance of the transcription factor KLF5 in cardiovascular remodeling
Nagai R, Suzuki T, Aizawa K, Shindo T, Manabe I
J ThrombHaemost, 343, 1569-1576, 2005

Synthetic Retinoid Am80 Suppresses Smooth Muscle Phenotypic Modulation and In-Stent Neointima Formation by Inhibiting KLF5
Fujiu K, Manabe I, Ishihara A, Oishi Y, Iwata H, Nishimura G, Shindo T, Maemura K, Kagechika H, Shudo K, Nagai R
Circ Res, 97, 1132-1141, 2005

Kruppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation
Oishi Y, Manabe I, Tobe K, Tsushima K, Shindo T, Fujiu K, Nishimura G, Maemura K, Yamauchi T, Kubota N, Suzuki R, Kitamura T, Akira S, Kadowaki T, Nagai R
Cell Metabolism, 1, 27-29, 2005

Protective effects of endogenous adrenomedullin on cardiac hypertrophy, fibrosis and renal damage
Niu P, Shindo T, Iwata H, Iimuro S, Takeda N, Zhang Y, Ebihara A, Suematsu Y, Kangawa K, Hirata Y, Nagai R
Circulation, 109, 1789-1794, 2004

Angiogenic effects of adrenomedullin in ischemia and tumor growth
Iimuro S, Shindo T, Moriyama N, Amaki T, Niu P, Takeda N, Iwata H, Zhang Y, Ebihara A, Nagai R
Circ Res, 95, 415-423, 2004

Unique roles of G protein-coupled histamine H2 and gastrin receptors in growth and differentiation of gastric mucosa
Fukushima Y, Matsui T, Saitoh T, Ichinose M, Tateishi K, Shindo T, Fujishiro M, Sakoda H, Shojima N, Kushiyama A, Fukuda S, Anai M, Ono H, Oka M, Shimizu Y, Kurihara H, Nagai R, Ishikawa T, Asano T, Omata M
Eur J Pharmacol, 502, 243-252, 2004

KLF5/BTEB2, a Kruppel-like zinc-finger type transcription factor, mediates both smooth muscle cell activation and cardiac hypertrophy.
Nagai R, Shindo T, Manabe I, Suzuki T, Kurabayashi M
Adv Exp Med Biol, 538, 57-65, 2003

Accelerated cardiac hypertrophy and renal damage induced by angiotensin II in adrenomedullin knockout mice.
Niu P, Shindo T, Iwata H, Ebihara A, Suematsu Y, Zhang Y, Takeda N, Iimuro S, Hirata Y, Nagai R
Hypertens Res, 26, 731-736, 2003

Targeted disruption of adrenomedullin and alphaCGRP genes reveals their distinct biological roles.
Kurihara H, Shindo T, Oh-Hashi Y, Kurihar Y, Kuwaki T
Hypertens Res, 26, S105-8, 2003

Endothelial responses of the aorta from adrenomedullin transgenic mice and knockout mice.
Nishimatsu H, Hirata Y, Shindo T, Kurihara H, Suzuki E, Sata M, Satonaka H, Takeda R, Nagata D, Kakoki M, Hayakawa H, Kangawa K, Matsuo H, Kitamura T, Nagai R
Hypertens Res, 26, S79-84, 2003

Structural and functional characterization of gastric mucosa and central nervous system in histamine H(2) receptor-null mice.
Fukushima Y, Shindo T, Anai M, Saitoh T, Wang Y, Fujishiro M, Ohashi Y, Ogihara T, Inukai K, Ono H, Sakoda H, Kurihara Y, Honda M, Shojima N, Fukushima H, Haraikawa-Onishi Y, Katagiri H, Shimizu Y, Ichinose M, Ishikawa T, Omata M, Nagai R, Kurihara H, Asano T
Eur J Pharmacol, 468(1), 47-58, 2003

Gene expression in fibroblasts and fibrosis. Involvement in cardiac hypertrophy.
Gene expression in fibroblasts and fibrosis. Involvement in cardiac hypertrophy.
Circ Res, 27, 1103-1113, 2002

Protective effect of adrenomedullin against cardiac hypertrophy and renal damage.
Niu P, Shindo T, Ebihara A, Zhang Y, Kawanami D, Imai Y, Amaki T, Suematsu Y, Nagai R
J Cardiac Failure, 8, S221, 2002

A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 null mutant mice develop renal lesions mimicking obstructive nephropathy.
Yokoyama H, Wada T, Kobayashi K, Kuno K, Kurihara H, Shindo T, Matsushima K
Nephrol Dial Transplant, 17, 39-41, 2002

Regulation of angiogenesis by the aging suppressor gene klotho.
Fukino K, Suzuki T, Saito Y, Shindo T, Amaki T, Kurabayashi M, Nagai R
Biochem Biophys Res Commun, 293, 332-7, 2002

Role of endogenous adrenomedullin in the regulation of vascular tone and ischemic renal injury. Studies on transgenic/knockout mice of adrenomedullin gene.
Nishimatsu H, Hirata Y, Shindo T, Kurihara H, Kakoki M, Nagata D, Hayakawa H, Satonaka H, Sata M, Tojo A, Suzuki E, Kangawa K, Matsuo H, Kitamura T, Nagai R
Circ Res, 90, 657-63, 2002

Attenuation of antigen-induced airway hyperresponsiveness in CGRP-deficient mice.
Aoki-Nagase T, Nagase T, Oh-Hashi Y, Shindo T, Kurihara Y, Yamaguchi Y, Yamamoto H, Tomita T, Ohga E, Nagai R, Kurihara H, Ouchi Y
Am J Physiol Lung Cell Mol Physiol, 283(5), L963-70, 2002

Krüppel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling.
Shindo T, Manabe I, Fukushima Y, Tobe K, Aizawa K, Miyamoto S, Kawai-Kowase K, Moriyama N, Imai Y, Kawakami H, Nishimatsu H, Suzuki T, Morita H, Maemura K, Sata M, Komukai M, Kagechika H, Kadowaki T, Kurabayashi M, Nagai R
Nature Medicine, 8(8), 856-63, 2002

Resistance to neointimal hyperplasia and fatty streak formation in adrenomedullin overexpression mice.
Imai Y, Shindo T, Maemura K, Sata M, Saito Y, Kurihara Y, Akishita M, Osuga J, Ishibashi S, Tobe K, Morita H, Oh-hashi Y, Suzuki T, Maekawa H, Kangawa K, Minamino N, Yazaki Y, Nagai R, Kurihara H
Arterioscler Thromb Vasc Biol, 22(8), 1310-5, 2002

Renal damage and salt-dependent hypertension in aged transgenic mice overexpressing endothelin-1.
Shindo T, Kurihara H, MaemuraK, Kurihara Y, Ueda O, Suzuki H, Kuwaki T, Ju K-H, Wang Y, Ebihara A, Nishimatsu H, Moriyama N, Fukuda M, Akimoto Y, Hirano H, Morita H, Kumada M, Yazaki Y, Nagai R, Kimura K
J. Mol. Med, 80(2), 105-16, 2002

Evidence for the physiological and pathological roles of adrenomedullin from genetic engineering in mice.
Imai Y, Shindo T, Maemura K, Kurihara Y, Ryozo N, Kurihara H
Ann N Y Acad Sci, 947, 26-33, 2001

Elevated sympathetic nervous activity in mice deficient in aCGRP.
Oh-hashi Y,* Shindo T* (*double 1st author), Kurihara Y, Imai T, Wang Y, Morita H, Imai Y, Kayaba Y, Nishimatsu H, Suematsu Y, Hirata Y, Yazaki Y, Nagai R, Kuwaki T, Kurihara H
Circ Res, 89, 983-90, 2001

Vascular abnormalities and elevated blood pressure in mice lacking adrenomedullin gene.
Shindo T, Kurihara Y, Nishimatsu H, Moriyama N, Kakoki M, Wang Y, Imai Y, Ebihara A, Kuwaki T, Ju K-H, Minamino N, Kangawa K, Ishikawa T, Fukuda M, Akimoto Y, Kawakami H, Imai T, Morita H, Yazaki Y, Nagai R, Hirata H, Kurihara H
Circulation, 104, 1964-71, 2001

Vascular smooth muscle cell-directed overexpression of heme oxygenase-1 elevates blood pressure through attenuation of nitric oxide-induced vasodilation in mice.
Imai T, Morita T, Shindo T, Nagai R, Yazaki Y, Kurihara H, Suematsu M, Katayama S
Circ Res, 89(1), 55-62, 2001

Diet-induced hyperhomocysteinemia exacerbates neointima formation in rat carotid arteries after balloon injury.
Morita H, Kurihara H, Yoshida S, Saito Y, Shindo T, Oh-Hashi Y, Kurihara Y, Yazaki Y, Nagai R
Circulation, 103(1), 133-9, 2001

ADAMTS-1: A metalloproteinase-disintegrin essential for normal growth, fertility, and organ morphologyand function.
Shindo T, Kurihara H, Kuno K, Yokoyama H, Wada T, Kurihara Y, Imai T, WangY, Oh-hashi Y, Morita H, Ishikawa T, Yazaki Y, Matsushima K
J Clin Invest, 105(10), 1345-52, 2000

Hypotension and resistance to lipopolysaccharide-induced shock in transgenic mice overexpressing
Shindo T, Kurihara H, Maemura K, Kurihara Y, Kuwaki T, Izumida T, Minamino N, Ju K, Morita H, Oh-hashi Y, Kumada M, Kangawa K, Nagai R, Yazaki Y
Circulation, 101(19), 2309-16, 2000

Evidence for association between paraoxonase gene polymorphisms and atherosclerotic diseases.
Imai Y, Morita H, Kurihara H, Sugiyama T, Kato N, Ebihara A, Hamada C, Kurihara Y, Shindo T, Oh-hashi Y, Yazaki Y
Atherosclerosis, 149(2), 435-42, 2000

Responses of blood pressure and catecholamine metabolism to high salt loading in endothelin-1 knockout mice.
Morita H, Kurihara H, Kurihara Y, Kuwaki T, Shindo T, Oh-hashi Y, Kumada M, Yazaki Y
Hypertens Res, 22(1), 11-6, 1999

Polymorphism of the methionine synthase gene : association with homocysteine metabolism and late-onset vascular diseases in the Japanese population.
Morita H, Kurihara H, Sugiyama T, Hamada C, Kurihara Y, Shindo T, Oh-hashi Y, Yazaki Y
Arterioscler Thromb Vasc Biol, 19(2), 298-302, 1999

Upregulation of endothelin-1 and adrenomedullin gene expression in the mouse endotoxin shock model.
Shindo T, Kurihara H, Kurihara Y, Morita H, Yazaki Y
J Cardiovasc Pharmacol, 31, S541-4, 1998

Cardiac sarcoidosis.
Shindo T, Kurihara H, Ohishi N, Morita H, Maemua K, Kurihara Y, Tsuneyoshi H, Chi H, Yamaoki K, Yazaki Y
Circulation, 97(13), 1306-7, 1998

Systemic and renal response to salt loading in endothelin-1 knockout mice.
Morita H, Kurihara H, Kurihara Y, Shindo T, Kuwaki T, Kumada M, Yazaki Y
J Cardiovasc Pharmacol, (31), S557-60, 1998

Methylenetetrahydrofolate reductase gene polymorphism and ischemic stroke in Japanese.
Morita H, Kurihara H, Tsubaki S, Sugiyama T, Hamada C, Kurihara Y, Shindo T, Oh-hashi Y, Kitamura K, Yazaki Y
Arterioscler Thromb Vasc Biol, 18(9), 1465-9, 1998

Genetic polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR) as a risk factor forcoronary artery disease.
Morita H, Taguchi J, Kurihara H, Kitaoka M, Kaneda H, Kurihara Y, Maemura K, Shindo T, Minamino T, Ohno M, Yamaoki K, Ogasawara K, Aizawa T, Suzuki S, Yazaki Y
Circulation, 95(8), 2032-6, 1997

Nitric oxide synthesis in cardiac myocytes and fibroblasts by inflammatory cytokines.
Shindo T, Ikeda U, Ohkawa F, Kawahara Y, Yokoyama M, Shimada K
Cardiovasc Res, 29(6), 813-9, 1995

Nitric oxide synthesis in rat cardiac myocytes and fibroblasts.
Shindo T, Ikeda U, Ohkawa F, Takahashi M, Funayama H, Nishinaga M, Kawahara Y, Yokoyama M, Kasahara T, Shimada K
Life Sci., 55(14), 1101-8, 1994

Methods Mol Biol. 1874
Sakurai T, Kamiyoshi A, Ohtsuka M, Gurumurthy CB, Sato M, Shindo T., Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line.
257-271 2019

Oocytes Maternal information and functions
Sakurai T, Shindo T, Sato M, Joint work, Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals
Springer, 495-510 2017

Results Probl Cell Differ. 63
Sakurai T, Shindo T, Sato M., Noninheritable maternal factors useful for genetic manipulation in mammals.
495-510 2017

Knockout mice and transgenic mice. Nishikimi T eds: Adrenomedullin in Cardiovascular Disease
Springer, New Jersey, pp 167-173 2005

Vascular homeostasis by the AM-RAMP2 system promotes T cell recruitment, prevents cancer cell migration, and suppresses lymph node metastasis.
Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Zhao Y, Matsuda Y, Zhang Y, Guo Q, Li P, Hoshiyama K, Li J, Onishi N, Hayashi M, Kasahara T, Shindo T
第88回日本循環器学会, 08 Mar. 2024

Protective effects of adrenomedullin 2 against heart failure by regulating the metabolism of cardiac myocytes
Zhao Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Matsuda Y, Zhang Y, Guo Q, Li P, Hoshiyama K, Li J, Onishi N, Hayashi M, Kasahara T, Shindo T
第88回日本循環器学会, 08 Mar. 2024

Metabolic regulation by the adrenomedullin2-RAMP3 system
Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Tanaka M, Zhao Y, Matsuda Y, Zhang Y, Guo Q, Li P, Hoshiyama K, Li J, Onishi N, Hayashi M, Kasahara T, Shindo T
第88回日本循環器学会, 08 Mar. 2024

アドレノメデュリン2は、心筋細胞のミトコンドリア機能と代謝系シグナルを制御し、心保護に働く
Zhao Yunlu、桜井敬之、神吉昭子、新藤優佳、河手久香、田中愛、松田順繁、Zhang Yan、Guo Qianqian、Li Peixuan、星山健、Li Jiake、大西菜穂、林真倫那、笠原智貴、新藤隆行
第53回日本心脈管作動物質学会, 09 Feb. 2024

アドレノメデュリンの受容体活性調節システムを標的とした癌転移の制御
田中愛、新藤隆行
第53回日本心脈管作動物質学会, 09 Feb. 2024, Invited

Adrenomedullin 2/intermedin is produced in response to pressure stress and protects cardiac function by regulating cardiomyocyte metabolism
Zhao Y､Shindo T
第40回国際心臓研究学会, 09 Dec. 2023

閉経後代謝障害におけるRAMP1、RAMP3の病態生理学的意義の検討
大西菜穂、神吉昭子、桜井敬之、新藤優佳、河手久香、田中愛、Yunlu Zhao、松田順繁、Yan Zhang、QianQian Guo、Peixuan Li、星山健、林真倫那、Li Jiake、新藤隆行
第44回日本肥満学会, 25 Nov. 2023

脂肪肝および肝癌発症における、アドレノメデュリン2(AM2)の病態生理学的意義
神吉昭子、桜井敬之、新藤優佳、河手久香、田中愛、Zhao Yunlu、松田順繁、Zhang Yan、Guo Qianqian、Li Peixuan、大西菜穂、星山健、林真倫那、Li Jiake、新藤隆行
第44回日本肥満学会, 25 Nov. 2023

AM-RAMP2系は、高内皮細静脈(HEV)の恒常性を制御し、リンパ節転移を抑制する
田中愛、桜井敬之、神吉昭子、新藤優佳、河手久香、ZHAO YUNLU、松田順繁、ZHANG YAN、GUO QIANQIAN、星山健、LI PEIXUAN、LI JIAKE、大西菜穂、林真倫那、笠原智貴、甲斐健佑、新藤隆行　
第6回日本腫瘍循環器学会　, 30 Sep. 2023

AM-RAMPシステムによる生体内恒常性制御
新藤隆行
第55回日本動脈硬化学会, 09 Jul. 2023, Invited

アドレノメデュリンの投与による、ぶどう膜炎の病態改善効果
松田順繁、柿原伸次、桜井敬之、神吉昭子、 市川優佳、田中愛、 河手久香、村田敏規、新藤隆行
第55回日本眼炎症学会, 07 Jul. 2023

RAMPシステムによる生体内恒常性制御機構の解明と応用展開
新藤隆行
第41回内分泌代謝学サマーセミナー　　　松尾賞受賞講演, 07 Jul. 2023, Invited

AM-RAMPシステムによる生体内恒常性制御
新藤隆行
第96回日本内分泌学会, 01 Jun. 2023, Invited

Investigation of the therapeutic effect of adrenomedullin on experimental autoimmune uveitis.
Matsuda Y, Kakihara S, Hirabayashi K, Imai A, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Kawate H, Zhao Y, Murata T, Shindo T
The Association for Research in Vision and Ophthalmology (ARVO) 2023, 23 Apr. 2023

Role of adrenomedullin-2/intermedin in the pathogenesis of neovascular AMD.
Kakihara S, Matsuda Y, Hirabayashi K, Imai A, Iesato Y, Sakurai T, Kamiyoshi A, Tanaka M, Ichikawa-Shindo Y, Kawate H, Zhao Y, Murata T, Shindo T
FujiRetina2023 　, 25 Mar. 2023

Endogenous adrenomedullin 2 /intermedin exerts cardioprotective effects against pressure overload.
Zhao Y, Sakurai T, Kamiyoshi K, Ichikawa-Shindo Y, Kawate H, Tanaka M, Kakihara S, Matsuda Y, Zhang Y, Guo Q, Li P, Onishi N, Kasahara T, Shindo T.
第87回日本循環器学会, 10 Mar. 2023

Adrenomedullin-2/intermedin ameliorates pathological angiogenesis and associated inflammation and fibrotic lesions via Meox2.
Kakihara S, Matsuda Y, Sakurai T, Kamiyoshi K, Tanaka M, Ichikawa-Shindo Y, Kawate H, Zhao Y, Zhang Y, Guo Q, Li P, Onishi N, Kasahara T, Shindo T.
第87回日本循環器学会, 10 Mar. 2023

Vascular homeostatic function of the AM-RAMP2 system and its application to the regulation of lymph node metastasis.
Tanaka M, Sakurai T, Kamiyoshi K, Ichikawa-Shindo Y, Kawate H, Kakihara S, Zhao Y, Matsuda Y, Zhang Y, Guo Q, Li P, Onishi N, Kasahara T, Shindo T.
第87回日本循環器学会, 10 Mar. 2023

Transcriptome analysis of the pathophysiological significance of adrenomedullin 2 in the mouse choroidal neovascularization model.
Kakihara K, Sakurai T, Kamiyoshi A, Tanaka M, Kawate H, Ichikawa-Shindo Y, Zhao Y, Matsuda Y, Hirabayashi K, Imai A, Iesato Y, Murata T, Shindo T.
The Association for Research in Vision and Ophthalmology (ARVO) 2022, 14 May 2022

Investigation of the therapeutic effect of adrenomedullin on experimental autoimmune uveitis.
Matsuda　Y, Kakihara S, Hirabayashi K, Imai A, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo, Y, Tanaka M, Kawate H, Zhao Y, Murata T, Shindo T.
The Association for Research in Vision and Ophthalmology (ARVO) 2022, 14 May 2022

Pathophysiological roles of adrenomedullin-2 in mouse model of ocular neovascularization.
Kakihara S, Tanaka M, Hirabayash K, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Kawate H, Ichikawa-Shindo Y, Tanaka M, Murata T, Shindo T.
The Association for Research in Vision and Ophthalmology (ARVO) 2021, 01 May 2021

Adrenomedullin-RAMP2 and RAMP3 systems regulate cardiac homeostasis against cardiovascular stresses
Cui N, Sakurai T, Kamiyoshi A, Shindo Y, Kawate H, Tanaka M, Dai K, Wei Y, Tanaka M. Kakihara S, Matsui S, Shindo T.
Asian Pacific Society of Cardiology Congress 2020 (APSC2020), 27 Jul. 2020

Pathophysiological significance of adrenomedullin-RAMP2 system in age-related macular degeneration
Masaaki Tanaka, Kazutaka Hirabayashi, Yasuhiro Iesato, Akira Imai, Yuichi Toriyama, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Megumu Tanaka, Akihiro Yamauchi, Toshinori Murata, Takayuki Shindo
Association for research in Vision and Ophthalmology, 28 Apr. 2019

Development of a retinal vascular occlusion model in mice and analysis of the pathophysiological roles of adrenomedullin
Kazutaka Hirabayashi, Toshinori Murata, Takayuki Shindo, Masaaki Tanaka, Akira Imai
Congress of Asia-Pacific Academy of Ophthalmology, 06 Mar. 2019

Investigation of the therapeutic effect of adrenomedullin on central retinal vein occlusion
Kazukata Hirabayashi, Toshinori Murata, Yasuhiro Iesato, Akira Imai, Yuichi Toriyama, Masaaki Tnanaka, Takayuki Sakurai, Akiko Kamiyoshi, Yuka Ichikawa-Shindo, Hisaka Kawate, Megumu Tanaka, Akihiro Yamauchi, Takayuki Shindo
The Association for Research in Vision and Ophthalmology, 28 Apr. 2018

Vascular endothelial adrenomedullin-RAMP2 system is essential for vascular integrity and organ homeostasis
Shindo T.
The Conference on Bioactive Peptides for Cell-Cell Communication 2014 Symposium, 11 Sep. 2014

Vasoactive peptides and their receptor regulation system.
新藤隆行
2010, Invited

Pathophysiological roles and potential applications of vasoactive peptides and their receptor regulation system.
新藤隆行
2010, Invited

Pathophysiological roles of Adrenomedullin-RAMP system in cardiovascular diseases
新藤隆行
2008, Invited

Adrenomedullin and cardiovascular disease　-Lessons from knockout mice-
新藤隆行
2005, Invited

Kruppel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling
新藤隆行
2003, Invited

Development of new therapy using animal models of atherosclerosis, hypertension, heart failure, renal failure and liver failure.

Pathophysiological roles of vasoactive peptides and their receptor system.

Regenerative medicine of blood vessel.

Generation of novel genetically engineerd mice.

Development of novel technique for genome editing

RAMP2を標的とする血管構造の安定化剤及び血管新生剤, EP1900374, Patent right
新藤隆行

RAMP2を標的とする血管構造の安定化剤及び血管新生剤, US 9,072,777B2, Patent right
新藤隆行

代謝症候群の治療もしくは予防剤、検査方法、検査薬、及び代謝症候群の治療薬の候補化合物のスクリーニング方法, CNZL200880117473.2, Patent right
新藤隆行

代謝症候群の治療もしくは予防剤、検査方法、検査薬、及び代謝症候群の治療薬の候補化合物のスクリーニング方法, US 8,858,915 B2, Patent right
新藤隆行

代謝症候群の治療もしくは予防剤、検査方法、検査薬、及び代謝症候群の治療薬の候補化合物のスクリーニング方法, EP2221065, Patent right

RAMP2を標的とする血管構造の安定化剤及び血管新生剤, 4993606, 304422, Patent right
新藤隆行

代謝症候群の治療もしくは予防剤、検査方法、検査薬、及び代謝症候群の治療薬の候補化合物のスクリーニング方法, 5099856, 071212　, Patent right
新藤隆行