TAKASHIMA Seiji

Academic Assembly School of Science and Technology Institute of Textile Science and Technology

Faculty of Textile Science and Technology Division of Applied Biology 

Associate Professor 

Degree

  • 博士(工学), 東京工業大学大学院

Research Keyword

    Stem cell, reproduction, regeneration, development, ageing, reprograming, infertility

Field Of Study

  • Regenerative medicine
  • Reproductive biology
  • Experimental medicine

TEL

    0268-21-5344

Mail Address

    stakashi★shinshu-u.ac.jp

Career

  • 2019
    信州大学 先鋭領域融合研究群バイオメディカル研究所 バイオテクノロジー分野 併任研究員
  • 2018
    信州大学 繊維学部 応用生物科学科 准教授
  • 2014
    信州大学 繊維学部 応用生物科学系 生物機能科学課程 助教(テニュアトラック)
  • 2007
    京都大学 医学部 遺伝医学講座 分子遺伝学分野 助教(常勤)
  • 2004
    東京大学医科学研究所 ヒト疾患モデル研究センター 細胞機能研究分野 リサーチフェロー

Educational Background

  • 2004, 東京工業大学大学院
  • 2001, 東京工業大学大学院
  • 1999, 東京工業大学

Award

  • 2015
    エルガ・ラボウォーター賞

Paper

  • (原著)Cdc42 is required for male germline niche development in mice.
    Yoshifumi Mori, Seiji Takashima, Mito Kanatsu-Shinohara, Zheng Yi, Takashi Shinohara
    Cell Rep., 36(7), 109550-109550, 17 Aug. 2021, Refereed
  • (原著)Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
    Akihiro Tsuchimoto, Masaaki Tone, Narumi Ogonuki, Masashi Hada, Atsuo Ogura & Seiji Takashima
    SCIENTIFIC REPORTS, 10, 10796, 01 Jul. 2020, Refereed
  • (原著)FGF2 has distinct molecular functions from GDNF in the mouse germline niche.
    Masaki K, Sakai M, Kuroki S, Jo J, Hoshina K, Fujimori Y, Oka K, Amano T, Yamanaka T, Tachibana M, Tabata Y, Shiozawa T, Ishizuka O, Hochi S, Takashima S.
    Stem Cell Reports, 10(6), 1782-1792, 19 Apr. 2018, Refereed
  • (原著)Expression dynamics of self-renewal factors for spermatogonial stem cells in the mouse testis.
    Sakai M, Masaki K, Aiba S, Tone M, Takashima S.
    J. Reprod. Dev., 64(3), 267-275, 16 Apr. 2018, Refereed
  • (総説)Culture and transplantation of spermatogonial stem cells.
    Takashima, S., Shinohara, T.
    Stem Cell Reports, 29, 46-55, 15 Mar. 2018, Refereed
  • (総説)Biology and manipulation technologies of male germline stem cells in mammals.
    Takashima, S.
    Reprod. Med. Biol., in press, 2018, Refereed
  • (総説/日本語)哺乳類精子幹細胞の試験管内操作技術.
    高島誠司
    日本胚移植学雑誌, 39, 143-149, 05 Aug. 2017, Refereed
  • (原著)Direct comparison of Cryotop® vitrification and Bicell® freezing on recovery of functional rat pancreatic islets.
    Yamanaka T, Tashima K, Takahashi R, Takashima S, Goto T, Hirabayashi M, Hochi S.
    Cryobiology, 73(3), 376-382, 01 Dec. 2016, Refereed
  • (国内学会発表受賞記念要旨収録)Differential expression of FGF2 and GDNF in the mouse testis
    Yagi, M., Takashima, S.
    J Reprod. Dev., 62(1), A8, 20 Feb. 2016
  • (原著)Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal.
    Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Morimoto, H., Inoue, K., Ogonuki, N., Jijiwa, M., Takahashi, M., Ogura, A., Shinohara, T.
    Stem Cell Reports, 4(3), 489-502, 10 Mar. 2015, Refereed
  • (原著)High revivability of vitrified-warmed bovine mature oocytes after recovery culture with α-tocopherol.
    Yashiro, I., Tagiri, M., Ogawa, H., Tashima, K., Takashima, S., Hara, H., Hirabayashi, M., Hochi, S.
    Reproduction, 149(4), 347-355, 27 Jan. 2015, Refereed
  • (原著)Regulation of pluripotency in male germline stem cells by Dmrt1
    Takashima, S., Hirose, M., Ogonuki, N., Ebisuya, M., Inoue, K., Morimoto, H., Kanatsu-Shinohara, M., Nishida, E., Ogura, A., Shinohara, T.
    Genes Dev., 27, 1949-1958, 2013, Refereed
  • (原著)Reconstitution of mouse spermatogonial stem cell niches in culture
    Kanatsu-Shinohara, M., Inoue, K., Takashima, S., Takehashi, M., Ogonuki, N., Morimoto, H., Nagasawa, T., Ogura, A., Shinohara, T.
    Cell Stem Cell, 11, 567-578, 2012, Refereed
  • (原著)Dynamic changes in EpCAM expression during spermatogonial stem cell differentiation in the mouse testis
    Kanatsu-Shinohara, M., Takashima, S., Ishii. K., Shinohara, T.
    PLOS ONE, 6, e23663, 2011, Refereed
  • (原著)Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier
    Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T.
    Cell Stem Cell, 9, 463-475, 2011, Refereed
  • (原著)Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation
    Kanatsu-Shinohara, M., Takashima, S., Shinohara, T.
    Proc. Natl. Acad. Sci. USA, 107, 6210-6215, 2010, Refereed
  • (原著)Genetic influences in mouse spermatogonial stem cell self-renewal
    Kanatsu-Shinohara, M., Ogonuki, N., Miki, H., Inoue, K., Morimoto, H., Takashima, S., Ogura, A., Shinohara, T.
    J. Reprod. Dev, 56, 145-153, 2010, Refereed
  • (原著)Phenotypic plasticity of mouse spermatogonial stem cells
    Morimoto, H., Kanatsu-Shinohara, M., Takashima, S., Chuma, S., Nakatsuji, N., Takehashi, M., Shinohara, T.
    PLOS ONE, 4, e7909, 2009, Refereed
  • (原著)Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation
    Lee, J., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Takashima, S., Oshimura, M., Toyokuni, S., Shinohara, T.
    Cell Stem Cell, 5, 76-86, 2009, Refereed
  • (原著)Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes
    Izumi-Yoneda, N., Toda, A., Okabe, M., Koike, C., Takashima, S., Yoshida, T., Konishi, I., Saito, S., Nikaido, T.
    Mol. Hum. Reprod., 15, 49-57, 2009, Refereed
  • (原著)Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
    Takashima, S., Takehashi, M., Lee, J., Chuma, S., Okano, M., Hata, K., Suetake, I., Nakatsuji, N., Miyoshi, H., Tajima, S., Tanaka, Y., Toyokuni, S., Sasaki, H., Kanatsu-Shinohara, M., Shinohara, T.
    Biol. Reprod., 81, 155-164, 2009, Refereed
  • (原著)Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin
    Kanatsu-Shinohara, M., Takehashi, M., Takashima, S., Lee, J., Morimoto, H., Chuma, S., Raducanu, A., Nakatsuji, N., Fässler, R., Shinohara, T.
    Cell Stem Cell, 3, 533-542, 2008, Refereed
  • (原著)Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells
    Kanatsu-Shinohara, M., Kato, M., Takehashi, M., Morimoto, H., Takashima, S., Chuma, S., Nakatsuji, N., Hirabayashi, M., Shinohara, T.
    Biol. Reprod., 79, 1121-1128, 2008, Refereed
  • (原著)Characterization of laminin isoforms in human amnion
    Takashima, S., Yasuo, M., Sanzen, N., Sekiguchi, K., Okabe, M., Yoshida, T., Toda, A., Nikaido, T.
    Tissue Cell, 40, 75-81, 2008, Refereed
  • (原著)Human amniotic epithelial cells possess hepatocyte-like characteristics and functions
    Takashima, S., Ise, H., Zhao, P., Akaike, T., Nikaido, T.
    Cell Struct. Funct., 29, 73-84, 2004, Refereed
  • (原著)Transplanted human amniotic epithelial cells express connexin 26 and Na-K-ATPase in the inner ear
    Yuge, I., Takumi ,Y., Koyabu, K., Hashimoto, S., Takashima, S., Fukuyama, T., Nikaido, T., Usami, S.
    TRANSPLANTATION, 77, 1452-1454, 2003, Refereed
  • (原著)Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel
    Ise, H., Takashima, S., Nagaoka, M., Ferdous, A., Akaike, T.
    Biotechnol. Lett., 21, 209-213, 1999, Refereed

MISC

  • (新学術領域・班会議)信州大学での?精子幹細胞研究
    高島誠司
    16 Jul. 2014, Not invited
  • (日本語総説)「羊膜細胞の再生医学への応用」
    二階堂敏雄、衛 俊萍、高島誠司、安尾将法、張 合林、趙 鵬、福山哲広
    信州医学雑誌, 52, 7-14, 2004
  • (日本語総説)「羊膜細胞を用いた再生医学」
    衛 俊萍、高島誠司、駒津光久、相沢 徹、二階堂敏雄
    胆と膵, 25(5), 285-289, 2004
  • (日本語総説)「肝前駆細胞の探索と単離−生体肝臓と胎盤付着物である羊膜組織中の肝前駆細胞を中心にー」
    伊勢裕彦、高島誠司、趙鵬、赤池敏宏、二階堂敏雄
    肝胆膵, 46(3), 345-353, 2003
  • (日本語総説)“特集:21世紀の再生医療最先端−肝臓・膵臓− 羊膜細胞を用いた再生医療の可能性”
    高島誠司、衛俊萍、趙鵬、二階堂敏雄
    再生医療, 1(2), 79-85, 2002
  • (日本語総説)「特集:人工肝臓研究−最近の進歩 I.各装置の工夫 4.肝細胞スフェロイドと人工肝臓」
    伊勢裕彦、高島誠司、赤池敏宏
    外科, 63(5), 528-532, 2001

Lectures, oral presentations, etc.

  • (国際学会発表)“Dysregulated interleukin-1 activity results in male infertility”
    Kaito Masaki, Shunta Sakanishi, Shigeru Kyuwa, Shigeru Kakuta, and Seiji Takashima
    31 Oct. 2017, Not invited
  • (国際学会発表)“Ambivalent behavior of fibroblast growth factor 2 in mouse germline niche”
    Kaito Masaki, Shunsuke Kuroki, Jun-ichiro Jo, Makoto Tachibana, Yasuhiko Tabata, Seiji Takashima
    27 Sep. 2017, Not invited
  • (国際学会発表)“The role of neuroendocrine system in regulating spermatogenesis”
    Shota Aiba, Haruhiko adachi, Kaito Masaki and Seiji Takashima
    27 Sep. 2017, Not invited
  • (国際学会発表)“Depletion of recipient germ cells compromises the spermatogenesis of transplanted testis tissue”
    Akihiro Tsuchimoto, Masaaki Tone and Seiji Takashima
    27 Sep. 2017, Not invited
  • (国際学会発表/ポスター)“Differential expression of self-renewal factors for spermatogonial stem cells in the mammalian testicular niche”
    Seiji Takashima, 、Kaito Masaki、Jun-ichiro Jo, and Yasuhiko Tabata
    ISSCR annual meating, 15 Jun. 2017
  • (国内学会発表・ポスター)FGF2による未分化型精原細胞制御/日本実験動物学会
    (高島誠司),正木魁人、城潤一郎,田畑泰彦
    日本実験動物学会, 17 May 2017
  • (国内学会発表・口頭)精子幹細胞自己複製因子 FGF2 の精巣内での役割/日本畜産学会第122回大会
    (高島誠司),正木魁人
    日本畜産学会, 29 Mar. 2017
  • (国内学会発表・口頭)2つの精子幹細胞自己複製因子の精巣内発現動態と機能の比較/第109回日本繁殖生物学会
    高島誠司、正木魁人、黒木俊介、立花誠、保地眞一、八木瑞貴
    日本繁殖生物学会, 12 Sep. 2016
  • (国内学会発表/ポスター)ヒト雄性不妊病態モデルの解析/第109回日本繁殖生物学会
    正木魁人、八木瑞貴、角田茂、坂西俊太、久和茂、高島誠司
    日本繁殖生物学会, 12 Sep. 2016
  • (国内学会発表/ポスター)精巣機能制御における末梢神経の役割の解明/第109回日本繁殖生物学会
    足立晴彦, 八木瑞貴, 高島誠司
    日本繁殖生物学会, 12 Sep. 2016
  • (国内学会発表/ポスター)抗ガン剤Busulfanが精子幹細胞ニッシェへ及ぼす負の影響/第109回日本繁殖生物学会
    刀根 将晃 高島 誠司
    日本繁殖生物学会, 12 Sep. 2016
  • (国内学会発表・口頭)2つの精子幹細胞自己複製因子の精巣内差次的発現とその制御メカニズムの解析/アンドロロジー学会
    正木魁人、八木瑞貴、黒木俊介、立花誠、藤森祐紀、保科和夫、岡賢二、天野俊康、塩沢丹里、石塚修、保地眞一、高島誠司
    日本アンドロロジー学会, 24 May 2016
  • (国際学会発表/ポスター)“Differential expression of self-renewal factors for spermatogonial stem cells in the mammalian testicular niche”
    Seiji Takashima, Mizuki Yagi, Kaito Masaki, Shunsuke Kuroki, Makoto Tachibana, Yuki Fujimori, Kazuo Hoshina, Kenji Oka, Toshiyasu Amano, Tanri Shiozawa, Osamu Ishizuka and Shinichi Hochi
    ISSCR annual meating, 24 May 2016
  • (国内学会発表・口頭)2つの精子幹細胞因子GDNFとFGF2の差次的発現の意義/再生医療学会
    八木瑞貴、正木魁人、黒木俊介、立花誠、藤森祐紀、保科和夫、岡賢二、天野俊康、塩沢丹里、石塚修、保地眞一、高島誠司
    日本再生医療学会, 19 Mar. 2016
  • (国内学会発表・口頭)精子幹細胞が示す2つの増殖様式/再生医療学会
    高島誠司
    日本再生医療学会, 18 Mar. 2016
  • (国際学会発表)“Dmrt1 suppress the pluripotency of male germline stem cells”
    Seiji Takashima, Michiko Hirose, Narumi Ogonuki, Miki Ebisuya, Kimiko Inoue, Hiroko Morimoto, Mito Kanatsu-Shinohara, Atsuo Ogura and Takashi Shinohara
    18 Feb. 2016, Not invited
  • (国際学会発表)“Glial cell line-derived neurotrophic factor independent self-renewal of murine spermatogonial stem cells”
    Seiji Takashima, Mito Kanatsu-Shinohara, Takashi Tanaka, Hiroko Morimoto, Kimiko Inoue, Narumi Ogonuki, Mayumi Jijiwa, Masahide Takahashi, Atsuo Ogura and Takashi Shinohara
    18 Feb. 2016, Not invited
  • (国際学会発表)“Differential expression of spermatogonial self-renewal factors in the mouse testis”
    Mizuki Yagi, Haruhiko Adachi, Masaaki Tone, Kaito Masaki, Yamato Kawahara, Hayato Ogawa and Seiji Takashima
    18 Feb. 2016, Not invited
  • (国際学会発表/ポスター)“Dmrt1 suppress the pluripotency of male germline stem cells”
    Seiji Takashima, Michiko Hirose, Narumi Ogonuki, Miki Ebisuya, Kimiko Inoue, Hiroko Morimoto, Mito Kanatsu-Shinohara, Atsuo Ogura and Takashi Shinohara
    International Symposium on Epigenome Dynamics and Regulation in Germ Cell P60-1, 17 Feb. 2016
  • (国際学会発表/ポスター)“Glial cell line-derived neurotrophic factor independent self-renewal of murine spermatogonial stem cells”
    Seiji Takashima, Mito Kanatsu-Shinohara, Takashi Tanaka, Hiroko Morimoto, Kimiko Inoue, Narumi Ogonuki, Mayumi Jijiwa, Masahide Takahashi, Atsuo Ogura and Takashi Shinohara
    International Symposium on Epigenome Dynamics and Regulation in Germ Cell P60-2, 17 Feb. 2016
  • (国際学会発表/ポスター)“Differential expression of spermatogonial self-renewal factors in the mouse testis”
    Mizuki Yagi, Haruhiko Adachi, Masaaki Tone, Kaito Masaki, Yamato Kawahara, Hayato Ogawa and Seiji Takashima
    International Symposium on Epigenome Dynamics and Regulation in Germ Cell P69, 17 Feb. 2016
  • (国内学会発表・口頭)精子幹細胞が有する潜在的分化多能性の分子メカニズム/第108回日本繁殖生物学会
    高島誠司
    18 Sep. 2015, Not invited
  • (国内学会発表・ポスター・優秀発表賞受賞)精子幹細胞自己複製因子FGF2の精巣内発現挙動解析/第108回日本繁殖生物学会
    八木瑞貴、高島誠司
    18 Sep. 2015, Not invited
  • (招待講演)精子幹細胞の新しい自己複製様式
    高島誠司
    第80回日本インターフェロン・サイトカイン学会, 16 Jul. 2015, Invited
  • (招待講演)アステラス病態代謝研究会 研究報告会
    高島誠司
    18 Oct. 2014, Invited
  • (国内学会発表・口頭)精子幹細胞が有する潜在的分化多能性の分子メカニズム/第07回日本繁殖生物学会
    高島誠司
    22 Aug. 2014, Not invited
  • (招待講演)培養精子幹細胞が有する潜在的分化多能性
    高島誠司
    第45回精子研究会, 11 Jan. 2014, Invited
  • (プロシーディング)“Alpha 1 antitypsin activity in preterm premature rupture of amniotic membranes”
    Yoneda, N., Toda, A., Okabe, M., Takashima, S., Yoshida, T., Konishi, I., Saito, S., Nikaido, T.
    PLACENTA 28 8-9 A37, 2007, Not invited
  • (国際学会発表)“Human amnion derived cells show characteristics of stem cell and hepatocyte,”
    Takashima, S., Zhao, P., Akaike, T., and Nikaido, T.
    The 1st international symposium on developmental biology and tissue engineering, 2003, Not invited
  • (国際学会発表)“3-D culture of hepatocytes in agarose gel for application to Bio-Artificial Liver system,”
    Takashima, S., Ise, H., and Akaike, T.,
    6th world biomaterials congress, 2000, Not invited
  • (国際学会発表)“Hepatocytes forming spheroid in agarose gel survive from apoptosis and maintain cell viability and expression of specific function for long-term,”
    Takashima, S., Ise, H., and Akaike, T.
    4th international conference on cellular engineering,, 1999, Not invited

Courses

  • 応用生物特別講義
    Shinshu University
  • 発生生物学
    Shinshu University
  • 新入生ゼミナール
    Shinshu University
  • 発生生物学
    Shinshu University
  • 新入生ゼミナール
    Shinshu University
  • 発生生物学
    Shinshu University
  • 新入生ゼミナール
    Shinshu University
  • 新入生ゼミナール
    Shinshu University
  • 生物科学基礎実験ⅡA
    Shinshu University
  • 生物科学基礎実験ⅡB
    Shinshu University
  • 生物科学基礎実験ⅡA
    Shinshu University
  • 生物科学基礎実験ⅡB
    Shinshu University
  • 生物科学基礎実験ⅡA
    Shinshu University
  • 生物科学基礎実験ⅡB
    Shinshu University
  • 生物科学基礎実験ⅡA
    Shinshu University
  • 生物科学基礎実験ⅡB
    Shinshu University

Affiliated academic society

  • Oct. 2015
    日本再生医療学会
  • May 2016
    日本実験動物学会
  • Apr. 2016
    日本アンドロロジー学会
  • Jun. 2018
    日本抗加齢医学会
  • Feb. 2016
    ISSCR
  • Jun. 2014
    The Society for Reproduction and Development

Research Themes

  • 精子幹細胞のバイオロジー
    31 Dec. 2015
  • 精子幹細胞のバイオロジー
    01 Sep. 2015
  • 精子幹細胞のバイオロジー
    01 Sep. 2015
  • 精子幹細胞のバイオロジー
    01 Sep. 2015
  • ヒト精子幹細胞培養法の確立
    01 Jan. 2015
  • ヒト精子幹細胞培養法の確立
    01 Jan. 2015
  • ヒト精子幹細胞培養法の確立
    01 Jan. 2015
  • ヒト精子幹細胞培養法の確立
    01 Jan. 2015
  • 家畜精子幹細胞の培養法確立
    01 Jun. 2014
  • 精子幹細胞のバイオロジー
    01 Apr. 2014
  • 精子幹細胞のバイオロジー
    01 Apr. 2014
  • 精子幹細胞のバイオロジー
    01 Apr. 2014
  • Biology of spermatogonial stem cell
  • Pluripotency potential of germline stem cell
  • Aging of stem cell system

Media Coverage

  • 不明
    読売新聞(2月13日夕刊 3面), 13 Feb. 2015, Paper
  • 京都大学 新たな「精子幹細胞」を発見
    関西テレビ, 13 Feb. 2015, Media report
  • 別タンパク質で精子幹細胞増殖 京大グループ解明
    京都新聞(2月13日 31面), 13 Feb. 2015, Paper
  • 新型の「精子幹細胞」を発見
    産経新聞(2月13日 30面), 13 Feb. 2015, Paper
  • 京大など、増殖の仕方が異なる2種類のGS細胞の存在を確認-男性不妊治療に活用へ
    日刊工業新聞(2月16日 21面), 13 Feb. 2015, Paper
  • 男性不妊症の治療に光 京大、精子の幹細胞に別タイプ発見
    日本経済新聞(2月13日 42面), 13 Feb. 2015, Paper
  • 京大チーム、発見 男性不妊症の治療に光
    KBS京都, 13 Feb. 2015, Media report
  • 精子幹細胞の新しい自己複製様式の発見 -男性不妊症の原因解明、遺伝病の発症機序の理解に期待-
    京都大学広報, 09 Feb. 2015, Others

Academic Contribution Activities

  • Planning etc
    日本繁殖生物学会第111回上田大会, 信州大学上田キャンパス, 12 Sep. 2018 - 16 Sep. 2018
  • J. Reprod. Dev, Mar. 2017 - May 2017
  • Scientific reports, Nov. 2016 - Nov. 2016
  • J. Reprod. Dev, Nov. 2016 - Nov. 2016
  • JARG, Jun. 2015 - Aug. 2015
  • J. Reprod. Dev, Apr. 2015 - Dec. 2015
  • J. Reprod. Dev, Mar. 2015 - Mar. 2015

Thesis guidance

  • 2017, 2, 0, 2, 0, 0, 2, 0, 0, 0, 0, 0, 0
  • 2014, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
  • 2015, 2, 0, 2, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
  • 2016, 3, 0, 3, 0, 0, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0