Faculty of Textile Science and Technology 

Professor Emeritus 


  • Doctor of Pharmaceutical Science

Research Keyword

    nucleic acid, DNA repair enzyme, cell wall (peptidoglycan), cell wall lytic enzyme, Bacillus subtilis, Molecular biology, Enzyme engineering

Field Of Study

  • Molecular biology, molecular biology

Educational Background

  • 1978 - 1983, Osaka University, Graduate School, Division of Pharmaceutical Sciences
  • 1974 - 1978, Osaka University, Faculty of Pharmaceutical Science


  • Recogunitoin model of uracil residues in DNA by AP endonuclease from Methanothermobacter thermatotrophicus
    N. Tabata, T. Kodera, R. Arai, and T. Shida
    Photon Factory Activity Report, 2012, #30 B, 2013
  • CD81and CD9 work independently as extracellular components upon fusion of sperm and oocyte
    N. Ohnami, A. Nakamura, M. Miyado,M. Sato, N. Kawano, K. Yoshida, Y. Harada, Y. Takezawa, S. Kanai, C. Ono, Y. Takahashi, K. Kimura, T. Shida,K. Miyado, and A. Umezawa
    Biology open, 1, 640-647, 2012
  • Incipent Complex Formation between AP Endonuclease and DNA contining AP site: A Vital Role of the Tryptophan Residue
    Y. Yamada, T. Kodera, K. Ohnishi, K. Kaneda, T. Shida
    Nucleic Acids Symposium, 53, 309-310, 2009
  • Identification and Characterization of Novel Cell WallHydrolase CwlT: a Two-domain Autolysin Exhabiting N-Acetylmuramidase and D,L-Endopeptidase Activities
    Fukushiama, T. Kitajima, H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, and J. Sekiguchi.
    J. Biol. Chem., 283, 11117-11125, 2008
  • Role of the tryptophan residue in the vicinity of the catalytic center of exonuclease III family AP endonucleases: AP site recognition mechanism
    K. Kaneda, J. Sekiguchi, and T. Shida
    Nucleic Acids Res., 34(5), 1552-1563, 2006WebofScience
  • Characterization of the AP Endonucleases from Thermoplasma volcanium and Lactobacillus plantarum;Contribution of Two Important Tryptophan Residues to AP Site Recognition
    K. Kaneda, K. Ohishi, J. Sekiguchi, and T. Shida
    Biosci.Biotechnol. Biochem., 70(9), 2213-2221, 2006WebofScience
  • Mutational analysis of catalyticsites of the cell wall lytic N-acetylmuramoyl-L-alanine amidase CwlC and CwlV1
    Toshio Shida/H. Hattori,/F. Ise /J. Sekiguchi
    J. Biol. Chem, 276(30), 28140-28146, 2001WebofScience
  • Overexpression, Purification, and Characterization of Bacillus subtilis N-Acetylmuramoyl-L-alanine Amidase CwlC,
    Toshio Shida/ H. Hattori/ F. Ise /J. Sekiguchi
    Biosci. Biotechnol. Biochem, 64(7), 1522-1525, 2000
  • Accumulation of a Recombinant Aspergillus oryzae Lipase artificially Localized on the Bacillus subtilis Cell Surface
    G. Kobayashi/ Toshio Shida/J. Sekiguchi
    J. Biosci. Bioeng, 90(4), 422-425, 2000WebofScience
  • Characterization of Bacillus subtilis ExoA Protein: a Multifunctional DNA-repair Enzyme Similar to the Echerichia coli Exonuclease III
    Toshio Shida/T. Ogawa/ J. Sekiguchi,
    Biosci. Biotechnol. Biochem, 63(9), 1528-1534, 1999WebofScience
  • Characterization of telomeric oligonucleotides: fidelity of repetitive nucleotide sequences
    Toshio Shida/M. Suda / J. Sekiguchi
    Nucleosides&Nucleotides, 17(1-3), 575-584, 1998WebofScience電子ジャーナル
  • A novel spectroscopic method for determining the ability of intermolecular association: deoxymononucleotidess and deoxydinucleotides
    M. Takayama/Toshio Shida / A. Tsugita
    Res. Comm. Biochem. Cell&Mol. Biol, 1(1-3), 185-203, 1997
  • Analysis of substrate specificity of the RuvC Holliday junction resolvase with synthetic Holliday junctions
    Toshio Shida/ Y. Kyogoku/ H. Shinagawa
    J. Biol. Chem, 271(42), 26105-26109, 1996WebofScience


  • DNA修復酵素APエンドヌクレアーゼ研究から見えてきた基質認識メカニズム
    生化学, 88(7), 618-623, 2011

Books and other publications

  • はじめて学ぶ繊維
    志田敏夫, 第2章の2
    日刊工業新聞社, 2011
  • はじめて学ぶ繊維
    志田敏夫, 第2章の2
    工業調査会, 2008

Lectures, oral presentations, etc.

  • Functional analysis of tetraspanin CD9 in sperm-egg fusion
    Naoko Ohnami, Hideki Tsumura, Ken Kimura, Toshio Shida, and Kenji Miyado
    6th International Conference on Advanced Fiber/Textile Materials 2011 in Ueda, 2011
  • Incipient complex formation between AP endonuclease and DNA containing AP site: A vital role of the tryptophan residue
    T.Shida, Y.Yamada, T.Kodera, K.Ohishi
    6th International Symposium on Nucleic Acids Chemistry, Sep. 2009

Research Themes

  • 生体高分子の分解に働く酵素の基質認識機構の解明