中村　美紀子｜信州大学研究者総覧

准教授（特定雇用）　

研究者情報

研究活動情報

Blending of selected yeast extract and peptone for inducible and constitutive protein production in Escherichia coli using the pET system
Mikiko Nakamura, Rinji Akada
Journal of Bioscience and Bioengineering, 138(6), 548-556, 2024年, 査読有り
筆頭著者, 責任著者

Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid.
Mikiko Nakamura, Hiroki Kikuta, Yukie Misumi, Ayako Suzuki, Hisashi Hoshida, Rinji Akada
J Biosci Bioeng, 133(6), 587-595, 2022年
筆頭著者, 責任著者

YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-mu m circle sequence in cir(0) strains of Saccharomyces cerevisiae
Misumi, Yukie; Nishioka, Satoko; Fukuda, Akira; Uemura, Takeshi; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
YEAST, 36(5), 249-257, 2019年05月

Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products
Nakamura, Mikiko; Aihara, Junya; Hoshida, Hisashi; Akada, Rinji;
Molecular Biotechnology, 60(12), 912-923, 2018年12月

End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter
Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Tomiyoshi, Keisuke; Hoshida, Hisashi; Akada, Rinji;
Molecular Biotechnology, 57(11-12), 1018-1029, 2015年12月

A synthetic medium optimized for growth and a minimal medium for fermentation in yeasts
Akada, Rinji; Misumi, Yukie; Nakagawa, Takaaki; Iwakiri, Ryo; Nakamura, Mikiko; Hoshida, Hisashi;
YEAST, 32, S228-S228, 2015年09月

A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells
Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Yarimizu, Tohru; Iwakiri, Ryo; Hoshida, Hisashi; Akada, Rinji;
Molecular Biotechnology, 57(8), 767-780, 2015年08月

Screening of accurate clones for gene synthesis in yeast
Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
Journal of Bioscience and Bioengineering, 119(3), 251-259, 2015年03月

Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus
Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
Microbial Cell Factories, 14, 2015年

Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus
Hoshida, Hisashi; Murakami, Nobutada; Suzuki, Ayako; Tamura, Ryoko; Asakawa, Jun; Abdel-Banat, Babiker M. A.; Nonklang, Sanom; Nakamura, Mikiko; Akada, Rinji;
YEAST, 31(1), 29-46, 2014年01月

Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae
Yarimizu, Tohru; Nonklang, Sanom; Nakamura, Junpei; Tokuda, Shuya; Nakagawa, Takaaki; Lorreungsil, Sasithorn; Sutthikhumpha, Surasit; Pukahuta, Charida; Kitagawa, Takao; Nakamura, Mikiko; Cha-aim, Kamonchai; Limtong, Savitree; Hoshida, Hisashi; Akada, Rinji;
YEAST, 30(12), 485-500, 2013年12月

Novel Small-Molecule Compounds That Affect Cellular Morphogenesis in Yeast and Mammalian Cells
Fukunaga, Tomoaki; Nakamura, Mikiko; Kitagawa, Takao; Watanapokasin, Ramida; Hoshida, Hisashi; Akada, Rinji;
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 77(8), 1669-1676, 2013年08月

Designed construction of recombinant DNA at the ura30 locus in the yeast Saccharomyces cerevisiae
Fukunaga, Tomoaki; Cha-aim, Kamonchai; Hirakawa, Yuki; Sakai, Ryota; Kitagawa, Takao; Nakamura, Mikiko; Nonklang, Sanom; Hoshida, Hisashi; Akada, Rinji;
YEAST, 30(6), 243-253, 2013年06月

生物工学 98 1
赤田倫治、中村美紀子、星田尚司, 続・生物工学基礎講座　バイオよもやま話　3番目のDNA連結反応 NHEJ: なんとほんとにeasyじゃん
日本生物工学会, 23-27 2020年

Bio Industry 36 9
赤田倫治、星田尚司、鈴木絢子、中村美紀子、藤本哲憲, 大容量PCR装置によるmLスケールでのDNA生産
シーエムシー出版, 58 2019年

酵母菌・麹菌・乳酸菌の産業応用展開 Advances in industrial applications of Yeast, Koji-molds and Lactic Acid bacteria
赤田倫治、中村美紀子、星田尚司, 第3章耐熱性酵母Kluyveromyces marxianusを用いた物質生産と育成技術
シーエムシー出版, 2018年01月30日
ISBN:9784781313177

Methods in Molecular Biology. DNA cloning and Assembly methods 1116
Nakamura Mikiko, Suzuki Ayako, Hoshida Hisashi, Akada Rinji, Minimum GC-rich sequences for overlap extension PCR and primer annealing
Springer Nature, 165-181 2014年

PCR-mediated expressions of a gene in triple hosts: yeast, E. coli, and mammalian cells
Nakamura Mikiko
PEPTALK2023, 2023年01月16日