Doctor of Agriculture, kyoto University

Applied microbiology, Applied microbiology

2004
Professor, Faculty of Textile Science and Technology, Shinshu University

1998 - 2004
Associate Professor, Faculty of Textile Science and Technology, Shinshu University

1996 - 1998
Associate Professor, Gene Research Center, Shinshu University

1993 - 1996
Associate Professor, Faculty of Textile Science and Technology, Shinshu University

1987 - 1993
Full-time Lecturer, Faculty of Textile Science and Technology, Shinshu University

1986 - 1987
Assistant Professor, Faculty of Textile Science and Technology, Shinshu University

1989 - 1989, Visiting Scholar, Cornell University , USA

1988 - 1988, Visiting Scholar, University of Notre Dame, USA

1984, Kyoto University, Graduate School, Division of Agriculture

1979, Kyoto University, Faculty of Agriculture

Identification and Characterization of Apigenin 6-C-Glucosyltransferase Involved in Biosynthesis of Isosaponarin in Wasabi (Eutrema japonicum)
Kyoko Mashima, Mayu Hatano, Hideyuki Suzuki, Makoto Shimosaka, Goro Taguchi
Plant Cell Physiol., 60(12), 2733-2743, Dec. 2019

Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum(common buckwheat)
Koja E, Ohata S, Maruyama Y, Suzuki H, Shimosaka M, Taguchi G.
Biosci Biotechnol Biochem, 82(10), 1790-1802, 2018, Refereed

C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants
Takamitsu Ito, Shunsuke Fujimoto, Fumiaki Suito, Makoto Shimosaka, Goro Taguchi
The Plant Journal, 91, 187-198, 2017

pFungiway: a series of plasmid vectors used for gene manipulation in fungi.
Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Toru Okuhara, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka
Ann. Microbiol., 66(2), 825–832, 2016

Taste characterization of the fruit body derived from a novel strain belonging to the genus Pleurotus.
Yoshihito Azumi, Kahori Doi, Kana Kogiso, Goro Taguchi, Makoto Shimosaka, Kenji Ouchi, Satoshi Inatomi
Mushroom Sci Biotechnol, 24(3), 129-135, 2016

Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis.
Miruku Ueda, Makoto Shimosaka and Ryoichi Arai
Acta Cryst, F71, 1516-1520, 2015

Crystal structure of ChiL, a chitinase from Chitiniphilus shinanonensis
Miruku Ueda, Makoto Shimosaka, Ryoichi Arai
Photon Factory Activity Report 2014, 32(B), 206, 2015

pFungiway: a series of plasmid vectors used for gene manipulation in fungi
Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka
Annals of Microbiology, 2015, Refereed

Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase.
Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.
Plant Biotech., 31, 519-524, 2014

Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.
Plant J., 80(3), 437–448, 2014

Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes
Shuichi Sekiya, Masato Yamada, Kou Shibata, Toru Okuhara, Masumi Yoshida, Satoshi Inatomi, Goro Taguchi, and Makoto Shimosaka
J. Biosci. Bioeng., 115(4), 360-365, 2013

キチン分解細菌Chitiniphilus shinanonensis由来の新規キチン分解酵素を用いたN-アセチルグルコサミンの生産
下坂　誠
バイオインダストリー, 30(4), 52-59, 2013

Knockdown of electron transfer flavoprotein b-subunit reduced TGF-b-induced a-SMA mRNA expression but not COL1A1 in fibroblast-populated three-dimensional collagen gel cultures
Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka
JOURNAL OF DERMATOLOGICAL SCIENCE, 68, 179-186, 2012

Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold, Aspergillus oryzae
Akihiro Sugita, Ayako Sugii, Kazuaki Sato, Xiao-Yong Zhang, An-Lan Dai, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 76(1), 193-195, 2012

Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase
Lanxiang Huang, Ewelina Garbulewska, Kazuaki Sato,Yuichi Kato, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
J. Biosci. Bioeng., 113(3), 293-299, 2012

Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium, Chitiniphilus shinanonensis
Lanxiang Huang, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 76(3), 517-522, 2012

バイオマスに由来する新規ナノ・ハイブリッドファイバーに対する微生物分解過程の解明
下坂　誠
IFO Research Communications, 25, 129-144, 2011

Chitin nanowhiskers mediate transformation of Escherichia coli by exogenous plasmid DNA
Alisa Mera, Jun Araki, Takashi Ohtsuki, Makoto Shimosaka, and Naoto Yoshida
J. Biotechnol. Biomater., 1(6), 114, 2011

Identification of ETFB as a candidate protein that participates in the mechanoregulation of fibroblast cell number in collagen gel culture.
Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka
JOURNAL OF DERMATOLOGICAL SCIENCE, 64, 119–126, 2011

Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of cultivated mushroom Flammulina velutipes
Tomomitsu Okamoto, Masato Yamada, Shuichi Sekiya, Toru Okuhara, Goro Taguchi, Satoshi Inatomi and Makoto Shumosaka
Biosci. Biotechnol. Biochem., 74(11), 2327-2329, 2010

Construction and analysis of a bacterial community exhibiting a strong chitinolytic activity
Kazuaki Sato, Yuichi Kato, Ayabi Fukamachi, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 74(3), 636-640, 2010

Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Kazuaki Sato, Yasuhito Azama, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Journal of Bioscience and Bioengineering, 109(5), 472-478, 2010

Agrobacterium tumefaciens-mediated transformation of antifungal lipopeptide producing fungus Coleophoma empetri F-11899
Masato Yamada, Kazunobu Yawata, Yohsuke Orino, Satoshi Ueda, Yasuhiro Isogai, Goro Taguchi, Makoto Shimosaka, and Seiji Hashimoto
Current Genetics, 55, 623-630, 2009

Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
Kazuaki Sato, Yuichi Kato, Goro Taguchi, Masahiro Nogawa, Akira Yokota, and Makoto Shimosaka;
J. Gen. Appl. Microbiol., 55(2), 147-153, 2009

Gene cloning, expression, and characterization ofa second b-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9
Xiqian Lan, Xin Zhang, Ritsuko Kodaira, Zeyang Zhou, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(2), 492-498, 2008

Characterization of a gene conferring red fluorescence isolated from an environmental DNA library constructed from soilbacteria
Xiqian Lan, Kazuaki Sato, Goro Taguchi, Zeyang Zhou, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(7), 1908-1914, 2008

Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression inPichia pastoris
Hiroshi Toda, Naoki Nagahata, Yoshihiko Amano, Kouichi Nozaki, Takahisa Kanda, Mitsuo Okazaki, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(12), 3142-3147, 2008

Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in thebasidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
Masato Yamada, Michihisa Kurano, Satoshi Inatomi, Goro Taguchi, Mitsuo Okazaki, and Makoto Shimosaka
FEMS Microbiol. Lett., 289(2), 130-137, 2008

Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
Masato Yamada, Sou Sakuraba, Kou Shibata, Goro Taguchi, Satoshi Inatomi, Mitsuo Okazaki, and Makoto Shimosaka
FEMS Microbiol. Lett., 254(1), 165-172, 2006

Analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis
Makoto Shimosaka, Kazuaki Sato, Naohide Nishiwaki, Takashi Miyazawa, and Mitsuo Okazaki
J. Biosci. Bioeng., 100(5), 545-550, 2005

ポイントがわかる分子生物学（第2版）3-10 ブロッティング（サザン、ノーザン、ウエスタン）
真野佳博・川向　誠編著, Joint work
丸善, 2010

応用生物学入門　第8章　微生物の有用物質
平林公男・白井孝治共編, Joint work
オーム社, 2010

キチン・キトサンの開発技術（平野茂博監修）　第７章「キトサナーゼ遺伝子のクローニングと解析」
Joint work
シーエムシー出版, 2009

Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis
Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013

Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – Development of gene disruption by an allelic exchange system –
Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013

Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – chiG, chiJ,and chiK coding for unique chitin-degrading enzymes –
Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013

Isolation of genes coding for chitin-degrading enzymes in the chitinolytic bacterium, Chitiniphilus shinanonensis
Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA
6th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda., 2011

Analysis of genes coding for chitin-degrading enzymes from the bacterium Chitiniphilus shinanonensis
Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA
International Conference of Future Textile, 2010

Agrobacterium tumefaciens-mediated transformation as a toolfor genomics in an industrially important fungus.
Masato Yamada, Kazuki Yawata1,Seiji Hashimoto and Makoto Shimosaka
The Society for Industrial Microbiology, The Annual Meeting and Exhibition 2009, Jun. 2009

Isolation and Characterization of a Novel Chitinolytic Bacterium, 
-Construction of Stable Chitinolytic Microbial Community-.
Sato K, Aoyama H, Kato Y, Nogawa M, Taguchi G, Yokota A, and Shimosaka M
7th East Asian Symposium on Polymers for Advanced Technology, 2009

Degradation of chitin by a novel bacterium
 Chitiniphilus shinanonensis originated from a bacterial community expressing a chitinolytic activity
Sato K, Shizume A, Aoyama H, Kato Y, Nogawa M, Taguchi G, and Shimosaka M
International Conference on Advanced Fiber/Textile Materials, 2009

Analysis of Bacterial Consortium Growing on Chitin Flake by Using Metagenomic Approach
Sato K., Azama Y., Kodaira R., Taguchi G., Shimosaka M
2007, The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda

Isolation, gene cloning, and characterization of the chitinolytic enzymes in the bacterium Aeromonas hydrophila strain SUWA-9
Lan, X., Zhang, X., Hu, J., and Shimosaka, M.
2007, The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda

Functional analysis of a novel C-terminal domain found in a chitosanase from the koji mold Aspergillus oryzae
Makoto Shimosaka, Ayako Sugii, Akihiro Sugita, Xiao-Yong Zhang, and Kazuaki Sato
Aug. 2006, 第10回キチン・キトサン国際会議

Applied Microbiology
Shinshu University

Genetic Engineering
Shinshu University

Food Engineering
Shinshu University

Fungal Molecular Biology Society of Japan

Japan Society for Bioscience, Biotechnology, and Agrochemistry

The Society for Biotechnology, Japan

Japanese Society for Chitin and Chitosan

Japanese Society of Mushroom Science and Biotechnology

未利用微生物資源の探索

微生物の生産するキチン・キトサン分解酵素に関する研究

糸状菌の遺伝子操作系の開発と有用菌株育種への応用