Ph.D., Kyoto Univ

Neuroscience-general, Neurophysiology / General neuroscience

Functional biochemistry, Functional biochemistry

Molecular biology, molecular biology

2024
Shinshu University, School of Medicine, Senior Assistant Professor

2019
Shinshu University, School of Medicine, Assistant Professor

2009
Assistant Professor, Shinshu University Graduate School of Medicine

1998 - 2009
理化学研究所　脳科学総合研究センター　研究員

1997 - 1998
岡崎国立共同研究機構　基礎生物学研究所　研究員

1995 - 1997
日本学術振興会特別研究員

1991 - 1997, Kyoto Univ., Grauate School of Science, Department of Biophysics

1986 - 1991, Kyoto Univ., Faculty of Science, Department of Biophysics

Deep-Learning-Based Analysis Reveals a Social Behavior Deficit in Mice Exposed Prenatally to Nicotine
Mengyun Zhou, Wen Qiu, Nobuhiko Ohashi, Lihao Sun, Marie-Louis Wronski,Emi Kouyama-Suzuki, Yoshinori Shirai, Toru Yanagawa, Takuma Mori and Katsuhiko Tabuchi
Cells, 13(3), 275, 2024, Refereed

Structural Analysis Implicates CASK-Liprin-alpha 2 Interaction in Cerebellar Granular Cell Death in MICPCH Syndrome
Guo, Qi; Kouyama-Suzuki, Emi; Shirai, Yoshinori; Cao, Xueshan; Yanagawa, Toru; Mori, Takuma; Tabuchi, Katsuhiko;
CELLS, 12(8), 1177, Apr. 2023, Refereed
Lead, Corresponding

An Epilepsy-Associated Mutation of Salt-Inducible Kinase 1 Increases the Susceptibility to Epileptic Seizures and Interferes with Adrenocorticotropic Hormone Therapy for Infantile Spasms in Mice
Pang, Bo; Mori, Takuma; Badawi, Moataz; Zhou, Mengyun; Guo, Qi; Suzuki-Kouyama, Emi; Yanagawa, Toru; Shirai, Yoshinori; Tabuchi, Katsuhiko;
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(14), 7927, Jul. 2022

IQSEC3 Deletion Impairs Fear Memory Through Upregulation of Ribosomal S6K1 Signaling in the Hippocampus
Kim, Dongwook; Jung, Hyeji; Shirai, Yoshinori; Kim, Hyeonho; Kim, Jinhu; Lim, Dongseok; Mori, Takuma; Lee, Hyojeong; Park, Dongseok; Kim, Hee Young; Guo, Qi; Pang, Bo; Qiu, Wen; Cao, Xueshan; Kouyama-Suzuki, Emi; Uemura, Takeshi; Kasem, Enas; Fu, Yu; Kim, Seungjoon; Tokunaga, Akinori; Yoshizawa, Takahiro; Suzuki, Tatsuo; Sakagami, Hiroyuki; Lee, Kea Joo; Ko, Jaewon; Tabuchi, Katsuhiko; Um, Ji Won;
Biological Psychiatry, 91(9), 821-831, 2022, Refereed
Lead

IQSEC2 Deficiency Results in Abnormal Social Behaviors Relevant to Autism by Affecting Functions of Neural Circuits in the Medial Prefrontal Cortex
Mehta, Anuradha; Shirai, Yoshinori; Kouyama-Suzuki, Emi; Zhou, Mengyun; Yoshizawa, Takahiro; Yanagawa, Toru; Mori, Takuma; Tabuchi, Katsuhiko;
CELLS, 10(10), 2724, Oct. 2021, Refereed
Lead

Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices
Suzuki, Tatsuo; Terada, Nobuo; Higashiyama, Shigeki; Kametani, Kiyokazu; Shirai, Yoshinori; Honda, Mamoru; Kai, Tsutomu; Li, Weidong; Tabuchi, Katsuhiko;
Life Science Alliance, 4(7), Jul. 2021, Refereed, Invited

Inhibition of CASK Expression by Virus-mediated RNA Interference in Medial Prefrontal Cortex Affects Social Behavior in the Adult Mouse
Xueshan Cao, Wen Qiu, Bo Pang, Mengyun Zhou, Anuradha Mehta, Qi Guo, Shirai Yoshinori, Mori Takuma, Tabuchi Katsuhiko
Shinshu Medical Journal, 69(1), 45-52, Feb. 2021, Refereed

Risperidone Mitigates Enhanced Excitatory Neuronal Function and Repetitive Behavior Caused by an ASD-Associated Mutation of SIK1
Badawi, Moataz; Mori, Takuma; Kurihara, Taiga; Yoshizawa, Takahiro; Nohara, Katsuhiro; Kouyama-Suzuki, Emi; Yanagawa, Toru; Shirai, Yoshinori; Tabuchi, Katsuhiko;
FRONTIERS IN MOLECULAR NEUROSCIENCE, 14, 2021, Refereed

Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons
Cao, Xueshan; Kouyama-Suzuki, Emi; Pang, Bo; Kurihara, Taiga; Mori, Takuma; Yanagawa, Toru; Shirai, Yoshinori; Tabuchi, Katsuhiko;
Biochemical and biophysical research communications, 533(3), 449-457, 2020, Refereed

Role of Splice Variants of Gtf2i, a Transcription Factor Localizing at Postsynaptic Sites, and Its Relation to Neuropsychiatric Diseases
Shirai, Yoshinori; Li, Weidong; Suzuki, Tatsuo;
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18(2), Feb. 2017, Refereed
Lead

Novel splice variants in the 5 ' UTR of Gtf2i expressed in the rat brain: alternative 5 ' UTRs and differential expression in the neuronal dendrites
Shirai, Yoshinori; Watanabe, Masahiko; Sakagami, Hiroyuki; Suzuki, Tatsuo;
JOURNAL OF NEUROCHEMISTRY, 134(3), 578-589, Aug. 2015, Refereed
Lead

Orexin-neuromodulated cerebellar circuit controls redistribution of arterial blood flows for defense behavior in rabbits
Nisimaru, Naoko; Mittal, Chetan; Shirai, Yoshinori; Sooksawate, Thongchai; Anandaraj, Prabu; Hashikawa, Tsutomu; Nagao, Soichi; Arata, Akiko; Sakurai, Takeshi; Yamamoto, Miyuki; Ito, Masao
Proceedings of the National Academy of Sciences of the United States of America, 110(35), 14124-14131, 27 Aug. 2013, Refereed

Lipid signaling in cytosolic phospholipase A(2)alpha-cyclooxygenase-2 cascade mediates cerebellar long-term depression and motor learning
Le, Tung Dinh; Shirai, Yoshinori; Okamoto, Takehito; Tatsukawa, Tetsuya; Nagao, Soichi; Shimizu, Takao; Ito, Masao
Proceedings of the National Academy of Sciences of the United States of America, 107(7), 3198-3203, 16 Feb. 2010, Refereed

Specific differential expression of phospholipase A(2) subtypes in rat cerebellum
Shirai, Y; Ito, M
JOURNAL OF NEUROCYTOLOGY, 33(3), 297-307, May 2004, Refereed
Lead, Corresponding

Induction of long-term depression in cerebellar Purkinje cells requires a rapidly turned over protein
Karachot, L; Shirai, Y; Vigot, R; Yamamori, T; Ito, M
JOURNAL OF NEUROPHYSIOLOGY, 86(1), 280-289, Jul. 2001, Refereed

Rapidly turned over protein maintains metabotropic synaptic transmission in Purkinje cells
Karachot, L; Shirai, Y; Vigot, R; Yamamori, T; Ito, M
NEUROREPORT, 11(13), 2903-2906, 11 Sep. 2000, Refereed

Suppression of ftsH Mutant Phenotypes by Overproduction of Molecular Chaperones.
Shirai, Y., Akiyama, Y. and Ito, K.
Journal of Bacteriology, 178, 1141-1145, 1996, Refereed
Lead

Involvement of FtsH in Protein Assembly into and through the Membrane.
Akiyama, Y., Shirai, Y. and Ito, K.
JOURNAL OF BIOLOGICAL CHEMISTRY, 269, 5225-5229, 1994, Refereed

Fusion of dioleoylphosphatidylcholine vesicles induced by an amphiphilic cationic peptide and oligophosphate at neutral pH.
Murata, M., Shirai, Y., Ishiguro, R., Kagiwada, S., Tahara, Y., Ohnishi, S. and Takahashi, S.
Biochim. Biophys. Acta, 1152, 99-108, 1993, Refereed

Specificity of amphiphilic anionic peptides for fusion of phospholipidvesicles.
Murata, M., Takahashi, S., Shirai, Y., Ishiguro, R., Kagiwada, S., Hishida, R. and Ohnishi, S.
BIOPHYSICAL JOURNAL, 64, 724-734, 1993, Refereed

“Neuroproteomics, second Edition”, from the NeuroMethods series of the Springer Protocol
Tatsuo Suzuki, Yoshinori Shirai and Weidong Li, Isolation of synapse sub-domains by subcellular fractionation using sucrose density gradient centrifugation – purification of the synaptosome, synaptic plasma membrane, postsynaptic density, synaptic membrane raft, and postsynaptic density lattice
Humana Press, 2019

Membrane Proteins: Structure, Function and Expression Control (ed. N. Hamasaki and K. Mihara, Kyushu Univ. Press/ Kager Medical and Scientific Publishers, Fukuoka and Basel)
Ito, K., Akiyama, Y., Kihara, A., Matsuo, E., Homma, T., Shirai, Y., Yoshihisa, T., Taura, T., Shimoike, T., Baba, T., Sone, M. and Matsumoto, G., The SecY complex and the FtsH complex in the E. coli plasma membrane.
169-181 1997

AI structural analysis implicates CASK-Liprin- interaction in cerebellar granule cell death in MICPCH syndrome
Qi Guo, Emi Kouyama-Suzuki, Yoshinori Shirai, Xueshan Cao, Toru Yanagawa, Takuma Mori, Katsuhiko Tabuch
第100回 日本生理学会（京都）, Mar. 2023

CASK plays a critical role in cerebellar granule cell survival
Qi Guo, Emi Suzuki-Kouyama, Yoshinori Shirai, Takuma Mori, Katsuhiko Tabuchi
日本生化学会中部支部年会, May 2022

New structure model for postsynaptic density, which contains tubulin as a key component
T. Suzuki, N. Terada, S. Higashiyama K. Kametani, Y. Shirai, M. Honda, T. Kai, W. Li, K. Tabuchi
Society for Neuroscience (SfN) annual meeting, Nov. 2021

PSDラティス:チュブリンを主体とする内部基本骨格構造と付随タンパク質
鈴木龍雄, 寺田信生, 東山繁樹, 亀谷清和, 白井良憲, 本田守, 甲斐力, 李衛東, 田渕克彦
第44回 日本神経科学会大会（神戸）, Jul. 2021

新規精製法により精製されたPostsynaptic density latticeのタンパク質成分の解析
鈴木龍雄, 東山繁樹, 田渕克彦, 白井良憲, 本田守, 甲斐力,李衛東
第43回 日本神経科学会大会, Jul. 2020

Characterization of PSD-lattice like structure purified from rat forebrain
T. Suzuki, S. Higashiyama, K. Tabuchi, Y. Shirai, W. Li
Society for Neuroscience (SfN) annual meeting (Chicago), Oct. 2019

Translational regulation of Gtf2i mRNA in neuronal dendrites by Pura, an RNA binding protein
Yoshinori Shirai, Weidong Li, Katsuhiko Tabuchi, Tatsuo Suzuki
第41回日本神経科学会大会（神戸コンベンションセンター）, Jul. 2018

Alternative 5'UTRs of Gtf2i mRNA, localization in neuronal dendrites via 5'UTR, and identification of RNA binding proteins specifically associate to the dendritic 5'UTR
Yoshinori Shirai, Weidong Li, Tatsuo Suzuki
第40回日本神経科学会大会（幕張メッセ）, Jul. 2017

Novel splice variants of Gtf2i expressed in the neuronal dendrites of rat brain.
Yoshinori Shirai, Tatsuo Suzuki
Society for Neuroscience (SfN) annual meeting, 2016.11, San Diego, USA, Nov. 2016

PSDに付随するASD関連遺伝子Rai1 mRNA: alternative 5’UTRs、mRNAの樹状突起への局在とRNA結合タンパク質
白井良憲、鈴木龍雄
第39回神経科学会大会 （パシフィコ横浜）, Jul. 2016

PSDに局在するASD関連遺伝子mRNAのalternative 5'UTRと脳での発現
白井良憲、鈴木龍雄
第38回日本神経科学会大会 (神戸、ポートアイランド), Jul. 2015

Gtf2i mRNAの新規な5'UTRの同定および脳での発現と神経細胞樹状突起への局在
白井良憲、鈴木龍雄
第37回日本神経科学大会（パシフィコ横浜）, Sep. 2014

CAGEデータベースを用いたGtf2iの新規な5'UTRの同定および脳での発現
白井良憲、鈴木龍雄
第36回日本分子生物学会年会（神戸ポートアイランド）, Dec. 2013

COX-2-induced PGD2/E2 play a crucial roles in cerebellar long-term depression
Le Dinh Tung, Yoshinori Shirai, Takao Shimizu, Masao Ito
Society for Neuroscience (SfN) annual meeting 2009, Chicago, USA, Oct. 2009

Dual Signal transduction pathway play a crucial role in cerebellar long-term depression
ル・ディン・ツン、白井良憲、清水孝雄、伊藤正男
第32回日本神経科学会大会（名古屋国際会議場）, Sep. 2009

小脳片葉P小葉はオレキシンニューロンを介して視床下部防御反応における循環調節に関与している
西丸直子、チェタン・ミッテル、白井良憲、トンチャイ・ソクサタ、藤井盛光、荒田晶子、桜井武、山本三幸、伊藤正男
第85回日本生理学会大会（東京）, Mar. 2008

Mlc1のmRNAはバーグマングリアの放射状突起に局在する
白井良憲、伊藤正男
第30回日本分子生物学会年会（パシフィコ横浜）, Dec. 2007

Orexinergic neurons mediate excitatory signals to Purkinje cells in a discrete microzone of the cerebellum
西丸直子、白井良憲、藤井盛光、桜井武、山本三幸、伊藤正男
第30回日本神経科学大会・第50回神経化学学会大会・第17回神経回路学会大会合同大会（パシフィコ横浜）, Sep. 2007

Mlc1 mRNA is localized in Bergmann glia radial fibers and binds with MARTA1 (MAP2 RNA trans-acting protein) in rat cerebellum
Yoshinori Shirai, Masao Ito
International Brain Research Organization (IBRO) World Congress 2007, Melbourne, Australia, Jul. 2007

Specific Involvement of a subtypes of cytosolc phospholipase A2-arachidonic acid-cox cascade in the induction of cerebellar long-term depression
Le Dinh Tung, Yoshinori Shirai, Takao Shimizu, Masao Ito
International Brain Research Organization 8IBRO) World Congrass 2007, Melbourne, Australia, Jul. 2007

Orexin-containing beaded cerebellar afferants mediate effects of stimulation of hypotharamic defense area on flocculus Purkinje cells in rabbits
Nisimaru, N., Mittal, C., Shirai, Y., Sooksawate, T., Gupta, M., Arata, A., Sakurai, T., Yamamoto, M., Ito, M.
Society for Neuroscience (SfN) annual meeting 2006, Atlanta, USA, Oct. 2006

Hypothalamic defense reaction involves Purkinje cells in the flocculus folium p via orexin and GABA
西丸直子、チェタン・ミッテル、トンチャイ・ソクサタ、白井良憲、桜井武、山本三幸、伊藤正男
第29回日本神経科学大会（国立京都国際会館）, Jul. 2006

Molecular and biochemical characterization of phospholipase A2 acitvating protein (PLAP) in rat brain
白井良憲、ル・ディン・ツン、山森哲雄、伊藤正男
第28回日本神経科学大会（パシフィコ横浜）, Jul. 2006

Cytosolic phospholipase A2 a subtype plays special role in induction of long-term depression in cerebellar Purkinje cell
ル・ディン・ツン、白井良憲、清水孝雄、伊藤正男
第28回日本神経科学大会（パシフィコ横浜）, Jul. 2006

Specific differential expression of phospholipase A2 subtypes in rat cerebellum and their specific roles in Purkinje cells
Yoshinori Shirai, Masao Ito
Gordon Research Conferences (Signal Transduction within the Nucleus), Santa Ynez Vallry, USA, Feb. 2005

ラット小脳におけるphosholipase A2 activating protein (PLAP)の機能解析
白井良憲、小野弘子、伊藤正男
第27回日本分子生物学会年会（神戸ポートアイランド）, Dec. 2004

ラット小脳におけるホスホリパーゼA2サブタイプの発現解析
白井良憲、小野弘子、ル・ディン・ツン、ラダワン・カラショ、伊藤正男
第26回日本分子生物学会年会（神戸ポートアイランド）, Dec. 2003

ユニット講義「神経（神経疾患）」
Shinshu University, School of Medicine

生理学（統御系）演習・実習
Shinshu University, School of Medicine

一般生理学特論
Shinshu University, Gradate School of Medicine

神経生理学特論
Shinshu University, Graduate School of Medicine

病態生理学特論
Shinshu University, Graduate School of Medicine

分子細胞生理学特別研究
Shinshu University, Graduate School of Medicine

分子細胞生理学論文演習
Shinshu University, Graduate School of Medicine

メンタルヘルス概論
Shinshu University, Graduate School oh Medicine

人間こころ心理学特論
Shinshu University, Graduate School of Medicine

神経科学研究方法特論
Shinshu University, Graduate School of Medicine

Introduction to Medical Science in Shinshu University Graduate School of Medicine
Shinshu University, Graduate School of Medicine

日本神経科学会

日本分子生物学会

日本生化学会

X連鎖性マウス遺伝学を利用したMICPCH症候群の神経病態メカニズムの解明
科学研究費補助金, 基盤 (B)
2023

PSD局在性核移行タンパク質を介したシナプス-核シグナリングの解析
科学研究費補助金, 基盤 (C)
2015

自閉症スペクトラム障害におけるGtf2iおよびRai1遺伝子の発現調節の役割
アイバワークス研究助成
2015

シナプスでの核タンパク質の局所翻訳・核への移行とシナプス可塑性
科学研究費補助金, 挑戦的萌芽研究
2011 - 2013

The 6th IBS (Institute for Biomedical Sciences) International Symposium; "Postsynaptic density-associated mRNAs related to neuropsychiatric diseases"
lecturer
Matsumoto, Japan, 2016

市民公開セミナー・疾患予防医学の現在と未来　「シナプス後部に局在する転写因子Gtf2i」
lecturer
信州大学, 20 Feb. 2014

第７回加齢適応医科学系専攻・市民公開講座　「脳と運動」
lecturer
松本文化会館, 26 Mar. 2011

第６回加齢適応医科学系専攻・市民公開講座　「脳の話」
lecturer
塩尻総合文化センター, 20 Feb. 2010

Peer review
IJMS Editorial Office, 2022

Peer review
JPM Editorial Office, 2021

Peer review
Genes Editorial Office, 2021

Peer review
Metabolites Editorial Office, 2021

Peer review
Elsevier Editorial System, 2009

Peer review
Springer Verlag, 2005