KOSHI Daishiro
Academic Assembly School of Science and Technology Institute of Engineering
Faculty of Engineering Materials Chemistry
Assistant Professor
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Paper
- Establishment of a CRISPR/Cas9 protocol by biolistic transformation of the filamentous basidiomycete Pleurotus ostreatus.
Yui Terakawa; Daishiro Koshi; Moriyuki Kawauchi; Takehito Nakazawa; Gi Hong An; Yoichi Honda
Journal of microbiological methods, 243, 107430-107430, Feb. 2026, Refereed
- Episomal maintenance of introduced plasmid with or without AMA1 sequence in the basidiomycete fungus Pleurotus ostreatus.
Daishiro Koshi; Moriyuki Kawauchi; Takehito Nakazawa; Yoichi Honda
Journal of bioscience and bioengineering, 141(5), 309-316, Feb. 2026, Refereed
Lead - Role of the putative transcription factor Rim101 in pH/oxidative stress response and cell wall structure formation in the white-rot fungus Pleurotus ostreatus
Saori Endo; Moriyuki Kawauchi; Yuitsu Otsuka; Junxian Han; Rio Sato; Daishiro Koshi; Kenya Tsuji; Akira Yoshimi; Chihiro Tanaka; Shigekazu Yano; Takehito Nakazawa; Yoichi Honda
Fungal Biology, 130(1), 101713-101713, Feb. 2026, Refereed - CRISPR/Cas9-directed disruption of wc-2 leads to the absence of fruiting body development in Pleurotus ostreatus.
Godai Toyonishi; Takehito Nakazawa; Daishiro Koshi; Masato Horii; Gi Hong An; Moriyuki Kawauchi; Yoichi Honda
FEMS microbiology letters, 372, Jan. 2025, Refereed - Trans-nuclei CRISPR/Cas9: safe approach for genome editing in the edible mushroom excluding foreign DNA sequences.
Daishiro Koshi; Junko Sugano; Fuga Yamasaki; Moriyuki Kawauchi; Takehito Nakazawa; Minji Oh; Yoichi Honda
Applied microbiology and biotechnology, 108(1), 548-548, Dec. 2024, Refereed
Lead - Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences.
Takehito Nakazawa; Moriyuki Kawauchi; Yuitsu Otsuka; Junxian Han; Daishiro Koshi; Kim Schiphof; Lucía Ramírez; Antonio G Pisabarro; Yoichi Honda
Applied microbiology and biotechnology, 108(1), 217-217, Feb. 2024, Refereed - Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection
Koshi, Daishiro; Ueshima, Hiroki; Kawauchi, Moriyuki; Nakazawa, Takehito; Sakamoto, Masahiro; Hirata, Mana; Izumitsu, Kosuke; Sumita, Takuya; Irie, Toshikazu; Honda, Yoichi
Journal of Wood Science, 68(1), 27, Mar. 2022, Refereed
Lead
MISC
- Development of CRISPR/Cas9 based trans-nuclei genome editing method for safe mushroom molecular breeding technology using the edible mushroom Pleurotus ostreatus
越大志朗; 菅野純子; 河内護之; 中沢威人; 坂本正弘; OH Minji; 本田与一
日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 74th, 2024 - A marker-free genome editing technique via transient gene expression in an edible mushroom, Pleurotus ostreatus.
越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一
日本木材学会大会研究発表要旨集(完全版)(CD-ROM), 72nd, 2022 - Function of an autonomously replicating sequence AMA1 in the basidiomycete Pleurotus ostreatus and its application to genome editing
越大志朗; 河内護之; 中沢威人; 坂本正弘; 本田与一
日本農芸化学会大会講演要旨集(Web), 2022, 2022 - A transient transformation and its application to gneome editing system in pleurotus ostreatus
越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一
日本分子生物学会年会プログラム・要旨集(Web), 44th, 2021
Lectures, oral presentations, etc.
- Episomal maintenance of plasmid with or without AMA1 sequence in the basidiomycete Pleurotus ostreatus
Daishiro Koshi; Moriyuki Kawauchi; Takehito Nakazawa; Yoichi Honda
33rd Fungal Genetics Conference, Mar. 2026,白色腐朽菌ヒラタケの形質転換におけるAMA1配列と導入プラスミドの解析
越大志朗; 河内護之; 中沢威人; 本田与一
日本農芸化学会 2026年度京都大会, Mar. 2026,食用担子菌ヒラタケを用いた、 核移行CRISPR/Cas9による 安全なきのこ分子育種技術の開発
越大志朗; 菅野純子; 河内護之; 中沢威人; 坂本正弘; OH Minji; 本田与一
第74回 日本木材学会, Mar. 2024,Trans-nuclei CRISPR/Cas9 for safe genome editing technology in Pleurotus ostreatus
Daishiro Koshi; Junko Sugano; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Minji Oh; Yoichi Honda
Asian Mycological Congress-AMC 2023, Oct. 2023,Trans-nuclei CRISPR/Cas9 for safe genome editing technology in Pleurotus ostreatus
Daishiro Koshi; Junko Sugano; Moriyuki Kawauchi; Takehito Nakazawa; Masahiro Sakamoto; Minji Oh; Yoichi Honda
16 th European Conference on Fungal Genetics, Mar. 2023Marker-free genome editing in the edible mushroom, Pleurotus ostreatus, using transient expression of genes required for CRISPR/Cas9 and for selection
Daishiro Koshi; Moriyuki Kawauchi; Toshikazu Irie; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda
International Union of Microbiological Societies - IUMS 2022, Jul. 2022Genome editing in mushroom-forming fungi with CRISPR/Cas9 technology
Yoichi Honda; Fuga Yamasaki; Daishiro Koshi; Takeshi Kobukata; Takehito Nakazawa; Moriyuki Kawauchi; Masahiro Sakamoto; Minji Oh
2022 KSM Spring International Meeting, Apr. 2022, Invitedヒラタケにおける一過性遺伝子発現を利用したマーカーフリーな ゲノム編集法の確立
越大志朗; 河内護之; 入江俊一; 中沢威人; 坂本正弘; 本田与一
第72回 日本木材学会大会, Mar. 2022食用キノコPleurotus ostreatusにおける一過性形質転換とそのゲノム;編集への応用
越 大志朗; 河内 護之; 入江 俊一; 中沢 威人; 坂本 正弘; 本田 与一
第44回 日本分子生物学会年会, Dec. 2021Transient transformation and its application to genome editing system in Pleurotus ostreatus
Daishiro Koshi; Moriyuki Kawauchi; Toshikazu Irie; Takehito Nakazawa; Masahiro Sakamoto; Yoichi Honda
Mushroom Science Virtual 2021, Oct. 2021担子菌Pleurotus ostreatusにおける自律複製配列AMA1の適用とそのゲノム編集への応用
越大志朗; 河内護之; 中沢威人; 坂本正弘; 本田与一
日本農芸化学会 2021年度仙台大会, Mar. 2021Research Themes
