Hosaka Takeshi
Academic Assembly School of Science and Technology Institute of Agriculture
Interdisciplinary Cluster for Cutting Edge Research Institute for Biomedical Sciences
Associate Professor
Researcher Information
Career
- 2019
信州大学 先鋭領域融合研究群 バイオメディカル研究所 生体分子イノベーション部門ユニット長 - 2016
大阪大学 生物工学国際交流センター 招聘准教授 - 2014
信州大学 先鋭領域融合研究群 バイオメデイカル研究所 准教授 - 2012
Faculty of Agriculture, Associate Professor - 2007 - 2012
Tenure-Track Assistant Professor, International Young Researchers Empowerment Center, Shinshu University, Japan - 2001 - 2007
Postdoctoral Fellow, MicrobialFunction Laboratory, National Food Research Institute, Japan
Educational Background
Research activity information
Award
- 2023
日本放線菌学会, The Society for Actinomycetes Japan - 2022
日本放線菌学会, The Society for Actinomycetes Japan - 2012
The society for actinomycetes Japan, The society for actinomycetes Japan Hamada Award - 2009
The Best Student Presentation Award, The Society for Actinomycetes Japan (2009) - 2005
The Japan Society for Bioscience Biotechnology and Agrochemistry New Project Award (2005) - 2003
The Best Poster Award, The Society for Actinomycetes Japan (2003)
Paper
- High-intensity green light potentially activates the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2)
Chompoonik Kanchanabanca, Takeshi Hosaka, Masanobu Kojima
Archives of Microbiology, 206, 2024, Refereed - Heterologous biosynthesis of myxobacterial lanthipeptides melittapeptins
Kaweewan I, Mukai K, Rukthanapitak P, Nakagawa H, Hosaka T, Kodani S
Applied Microbiology and Biotechnology, 108, 2024, Refereed - Heterologous production and structure determination of a new lanthipeptide sinosporapeptin using a cryptic gene cluster in an actinobacterium Sinosporangium siamense
Saito K, Mukai K, Kaweewan I, Nakagawa H, Hosaka T, Kodani S
Journal of Microbiology, 61, 641-648, 2023, Refereed, Not invited - Phenomenological interpretations of the mechanism for the concentration-dependent positive effect of antibiotic lincomycin on Streptomyces coelicolor A3(2)
Mukai K, Shibayama T, Imai Y, Hosaka T
Applied and Environmental Microbiology, 89(10), 2023, Refereed
Last, Corresponding - Unique physiological and genetic features of ofloxacin-resistant Streptomyces mutants
Hoshino K, Hamauzu R, Nakagawa H, Kodani S, Hosaka T
Applied and Environmental Microbiology, 88(3), 2022, Refereed
Last, Corresponding - Lincomycin-induced secondary metabolism in Streptomyces lividans 66 with a mutation in the gene encoding the RNA polymerase beta subunit
Mukai K, Kobayashi M, Hoshino K, Maruyama T, Hayashi D, Hamauzu R, Hosaka T
CURRENT MICROBIOLOGY, 77, 2933-2939, 2020, Refereed
Last, Corresponding - A putative mechanism underlying secondary metabolite overproduction by Streptomyces strains with a 23S rRNA mutation conferring erythromycin resistance
Hoshino K, Imai Y, Mukai K, Hamauzu R, Ochi K, Hosaka T
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 104(5), 2193-2203, 2020, Refereed
Last, Corresponding - Isolation and structure determination of a new cytotoxic peptide curacozole from Streptomyces curacoi based on genome mining
Kodani S, Kaweewan I, Komaki H, Hemmi H, Hoshino K, Hosaka T, Isokawa G, and Oyoshi T
The Journal of Antibiotics, 72, 1-7, 2019, Refereed - A possible mechanism for lincomycin induction of secondary metabolism in Streptomyces coelicolor A3(2)
Ishizuka M, Imai Y, Mukai K, Shimono K, Hamauzu R, Ochi K, Hosaka T
Antonie van Leeuwenhoek, 111, 705-716, 2018, Refereed
Last, Corresponding - Combined Drug-Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans
Funane K, Tanaka Y, Hosaka T, Murakami K, Miyazaki T, Shiwa Y, Gibu S, Inaoka T, Kasahara K, Fujita N, Yoshikawa H, Hiraga Y, Ochi K
Journal of Bacteriology, 200, e00188-18, 2018, Refereed - 抗生物質ホルミシスの理解と応用
保坂 毅
生物工学会誌（大村智先生ノーベル賞受賞記念特集−微生物由来天然物の実用化と未来−）, 94, 399-400, 2016
Lead, Corresponding - Lincomycin at subinhibitory concentrations potentiates secondary metabolite production by Streptomyces spp.
Imai Y, Sato S, Tanaka Y, Ochi K, Hosaka T
Applied and Environmental Microbiology, 81, 3869-3879, 2015, Refereed
Last, Corresponding - New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters
Ochi K. and Hosaka T.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 97, 87-98, 2013, Refereed - Development of the ability to produce secondary metabolites in Streptomyces through the acquisition of erythromycin resistance
Imai Y., Fujiwara T., Ochi K., and Hosaka, T.
The Journal of Antibiotics, 65, 323–326, 2012, Refereed
Last, Corresponding - Heterologous expression of plant RelA/SpoT homolog results in increased stresstolerance inSaccharomyces cerevisiae by accumulating the bacterial alarmone ppGpp
Ochi K., Nishizawa T., Inaoka T., Yamada Y., Hashimoto K., Hosaka T., Okamoto S., Ozeki, Y.
Microbiology, 158, 2213- 2224, 2012, Refereed - Rare earth elements activate the secondary metabolite-biosynthesis gene clusters in Streptomyces coelicolor A3(2)
Tanaka, Y., Hosaka T., and Ochi, K.
J Antibiot, 63, 477-481, 2010, Refereed - Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12
Hosaka T., Ohnishi-Kameyama M., Muramatsu H., Murakami K., Tsurumi Y., Kodani S., Yoshida M., Fujie A., and Ochi K..
NATURE BIOTECHNOLOGY, 27(5), 462-464, 2009, Refereed
Lead
- Bacterialalarmone, guanosine 5'-diphosphate 3'-diphosphate (ppGpp), predominantly binds the beta' subunit of plastid-encoded plastid RNA polymerasein chloroplasts.
Sato M., Takahashi K, Ochiai Y, Hosaka T, Ochi K, Nabeta K
ChemBioChem, 10, 1227-1233, 2009, Refereed - Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations
Wang G., Hosaka T., and Ochi K.
Appl Environ Microbiol, 74, 2834-2840, 2008 - Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants
Nishimura K., Johansen SK., Inaoka T., Hosaka T., Tokuyama S., Tahara Y., Okamoto S., Kawamura F., Douthwaite S., and Ochi K. .
J Bacteriol, 189, 6068-6073, 2007 - Mutations in rsmG, encoding a16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2)
Nishimura, K., Hosaka T., Tokuyama S., Okamoto S., and Ochi K.
J Bacteriol, 189, 3876-3883, 2007 - Increased expression of ribosome recycling factor is responsible for the enhanced protein synthesis during the late growth phase in an antibiotic-overproducing Streptomyces coelicolor ribosomal rpsL mutant
Hosaka, T., J. Xu, and K. Ochi
Mol Microbiol, 61, 883-897, 2006, Refereed
Lead - Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus
Kasai, K., T. Nishizawa, K. Takahashi, T. Hosaka, H. Aoki and K. Ochi
J Bacteriol, 188, 7111-7122, 2006 - EshA accentuates ppGpp accumulation andis conditionally required for antibiotic production in Streptomyces coelicolor A3(2)
Saito, N., J. Xu, T. Hosaka, S. Okamoto, H.Aoki, M. J. Bibb and K. Ochi.
J Bacteriol, 188, 4952-4961, 2006 - Improvement of alpha-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168
Kurosawa, K., T. Hosaka, N. Tamehiro, T. Inaoka and K. Ochi
Appl Environ Microbiol, 72, 71-77, 2006 - The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli
Hosaka, T., N. Tamehiro, N. Chumpolkulwong, C. Hori-Takemoto, M. Shirouzu, S. Yokoyama and K.Ochi
Mol Genet Genomics, 271, 317-324, 2004, Refereed
Lead - Ribosome engineering and secondary metabolite production
Ochi, K., S. Okamoto, Y. Tozawa, T. Inaoka, T. Hosaka, J. Xu and K. Kurosawa
Adv Appl Microbiol, 56, 155-184, 2004 - Structural basis for transcription regulation by alarmone ppGpp
Artsimovitch, I., V. Patlan, S. Sekine, M. N. Vassylyeva, T. Hosaka, K. Ochi, S. Yokoyama and D. G. Vassylyev
Cell, 117, 299-310, 2004 - Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis
Chumpolkulwong, N., C. Hori-Takemoto, T. Hosaka, T. Inaoka, T. Kigawa, M. Shirouzu, K. Ochi and S. Yokoyama
Eur J Biochem, 271, 1127-1134, 2004 - An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2)
Okamoto-Hosoya, Y., T. Hosaka and K. Ochi
Microbiology, 149, 3299-309, 2003 - Innovative approach for improvement of an antibiotic-overproducing industrial strain of Streptomyces albus
Tamehiro, N., T. Hosaka, J. Xu, H. Hu, N. Otake and K. Ochi
Appl Environ Microbiol, 69, 6412-6417, 2003 - Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2)
Okamoto, S., A. Lezhava, T. Hosaka, Y. Okamoto-Hosoya and K. Ochi
J Bacteriol, 185, 601-609, 2003 - Acetylacetoin synthase as a marker enzyme for detecting the 2,3-butanediol cycle
Ui, S., T. Hosaka, K. Mizutani, T. Ohtsuki and A. Mimura
J Biosci Bioeng, 93, 248-251, 2002 - Characterization of the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase gene from Bacillus cereus YUF-4
Hosaka, T., S. Ui, T. Ohtsuki, A. Mimura, M. Ohkuma and T. Kudo
J Biosci Bioeng, 91, 539-544, 2001, Refereed - Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4
Hosaka, T., S. Ui and A. Mimura
Biosci Biotechnol Biochem, 63, 199-201, 1999
Lead - Purification andproperties of acetylacetoin synthase from Bacillus sp. YUF-4
Ui, S., T. Hosaka, K. Mizutani and A. Mimura
Biosci Biotechnol Biochem, 62, 795-797, 1998 - Discovery of a new mechanism of 2,3-butanediol stereoisomer formation Bacillus cereus YUF-4
Ui, S., T. Hosaka, K. Watanabe, and A. Mimura
J Biosci Bioeng, 85, 79-83, 1998
MISC
- 放線菌の二次代謝に対する抗生物質の濃度依存的活性化作用
保坂毅、向井慶一郎、石塚美咲、小林桃子
月刊アグリバイオ, 30 Nov. 2018 - 放線菌における抗生物質のホルミシス効果の解析と応用
保坂 毅，今井 優
バイオサイエンスとインダストリー, 74, 516-518, 2016 - 放線菌の潜在能力発現に関わる薬剤耐性変異の特性解析と抗生物質発掘への応用
保坂 毅
日本放線菌学会誌, 28(2), 9-14, 2014 - 放線菌の転写及び翻訳系の改変による潜在能力の開発と抗生物質の探索
保坂毅, 今井優, 藤原達也
信州大学農学部紀要, 48, 9-15, 27 Mar. 2012 - 微生物の「休眠遺伝子」を目覚めさせて新抗生物質を発見
越智幸三・保坂毅・亀山真由美・村松秀行・村上果菜・鶴海泰久・小谷真也・吉田充・藤江昭彦
農林水産技術研究ジャーナル, 33(3), 22-25, 2010 - 2010 年度日本放線菌学会大会感想記
保坂毅
日本放線菌学会誌, 24(2), 17-18, 2010 - 放線菌の『休眠遺伝子』覚醒技術の開発
越智幸三・保坂毅・田中幸徳
バイオサイエンスとインダストリー, 67(8), 413-417, 2009 - リボゾーム工学による微生物からの有用物質発見
保坂毅, 王国君, 岡本晋, 越智幸三
月刊バイオインダストリー, 24(10), 59-66, 2007 - 放線菌を利用して多量の抗生物質をつくる
保坂毅, 為広紀正, 越智幸三
化学と生物, 42, 636-638, 2004
Lectures, oral presentations, etc.
- Molecular physiological studies on actinomycetes for a fundamental understanding of antibiotics
保坂毅（invited）
第93回日本細菌学会総会シンポジウム, 19 Feb. 2020, Invited抗生物質の本質的理解とその応用
保坂毅（invited)
微生物ウィーク2019, 25 Jul. 2019, InvitedAnalysis of mechanisms for antibiotic induction of secondary metabolism
SHIMONO K, ISHIZUKA M, IMAI Y, OCHI K, and HOSAKA T
18th International Symposium on the Biology of Actinomycetes (JEJU/KOREA), 2017抗生物質の濃度依存的現象「抗生物質ホルミシス」の理解と応用
保坂 毅 (Invited)
第68回日本生物工学会大会シンポジウム, 2016抗生物質ホルミシスの分子生物学的機構の理解と放線菌の二次代謝研究への応用
保坂 毅（Invited)
第10回ゲノム微生物学会年会シンポジウム, 2016抗生物質ホルミシスの分子生物学的機構の解析とその応用
保坂 毅（Invited）
第5回遺伝研研究会, 2015Ribosome-targeting antibiotics at subinhibitory concentrations potentiate secondary metabolite production by streptomycetes
Hosaka T（Invited）
Society for Industrial Microbiology and Biotechnology Annual Meeting, 2015Activation of secondary metabolite production in Streptomyces spp. by exposure to subinhibitory concentrations of lincomycin.
Imai Y., Sato S., Tanaka Y., Ochi K., and Hosaka T.
IUMS2014 [XIVth International Congress of Bacteriology and Applied Microbiology], 2014Activation of the potential to produce secondary metabolites in actinomycetes by modulating the RNA polymerase and/or ribosome
Hosaka T (invited)
ISIM2014 (Dalian/China）, 2014Lincomycin at subinhibitory concentrations activates the potential of Streptomyces strains to produce secondary metabolites
Imai Y., Sato S., Tanaka Y., Ochi K., and Hosaka T.
17th International Symposium on the Biology of Actinomycetes [ISBA’17]（Aydin/Turkey）, 2014潜在遺伝子活性化による有用放線菌の創出
保坂毅 (invited)
平成 21 年度科学交流フォーラム 第 11 回静岡ライフサイエンスシンポジウム, 2010, Invited微生物の潜在遺伝子活性化による有用二次代謝産物の探索
保坂毅 (invited)
山梨大学学術研究会, 2010放線菌の潜在的な抗生物質生産力を活性化するRNA ポリメラーゼ変異とリボソーム変異
保坂毅 (invited)
日本農芸化学会中部支部 第 157 回例会 若手シンポジウム, 2009, InvitedActivation of silent genes in Streptomyces sp. 631689 by generating a spontaneous gentamicin-resistant mutant
Hosaka, T., T. Fujiwara, Y. Imai, K. Sen and K. Ochi
15th International Symposium on the Biology of Actinomycetes, 2009The impact of “ribosome engineering” on antibiotic discovery
Hosaka T (invited)
Japan-UK Workshop 2008 (Genomics of Antibiotic-producing Actinomycete: Implicationsand Applications), 2008, InvitedAffiliated academic society
Research Themes
- リボソーム攻撃性抗生物質の活用による糸状菌の潜在的二次代謝能活性化技術の確立
科学研究費補助金, 挑戦的萌芽研究
2014 - 2016 - 放線菌の転写・翻訳系改変による潜在能力活性化機構の解明と二次代謝産物発掘への応用
科学研究費補助金, 若手研究（Ａ）
2013 - 2015 - 薬剤耐性付与による放線菌からのRNAポリメラーゼ変異株の分離と新抗生物質の探索
科学研究費補助金, 若手研究 (B)
2009 - 2011 - 放線菌の潜在遺伝子を覚醒させる薬剤耐性変異を活用した抗生物質探索技術の開発 (ホクト生物科学振興財団研究奨励金)
ホクト生物科学振興財団
2009 - 微生物の潜在機能誘発メカニズムと有用天然物の効率的生産を目指した「リボゾーム工学」技術の活用 (日本農芸化学会研究資金)
日本農芸化学会
2005 - Molecular physiological studies on actinomycetes for a fundamental understanding of antibiotics
- Fundamental and applied research in ribosome engineering
- Exploitation of novel antimicrobial compounds from various microbial sources and its applications
