大容量PCR装置によるmLスケールでのDNA生産, Bio Industry 36 9, 58 シーエムシー出版 2019 Author:赤田倫治、星田尚司、鈴木絢子、中村美紀子、藤本哲憲
第3章 耐熱性酵母Kluyveromyces marxianusを用いた物質生産と育成技術, 酵母菌・麹菌・乳酸菌の産業応用展開 Advances in industrial applications of Yeast, Koji-molds and Lactic Acid bacteria シーエムシー出版 2018(Jan. 30) Author:赤田倫治、中村美紀子、星田尚司
Minimum GC-rich sequences for overlap extension PCR and primer annealing, Methods in Molecular Biology. DNA cloning and Assembly methods 1116, 165-181 Springer Nature 2014 Author:Nakamura Mikiko, Suzuki Ayako, Hoshida Hisashi, Akada Rinji
論文 Triple gene expressions in yeast, Escherichia coli, and mammalian cells by transferring DNA fragments amplified from a mother yeast expression plasmid. J Biosci Bioeng,133(6):587-595 2022 Author:Mikiko Nakamura, Hiroki Kikuta, Yukie Misumi, Ayako Suzuki, Hisashi Hoshida, Rinji Akada
YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-mu m circle sequence in cir(0) strains of Saccharomyces cerevisiae YEAST,36(5):249-257 2019(May) Author:Misumi, Yukie; Nishioka, Satoko; Fukuda, Akira; Uemura, Takeshi; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products MOLECULAR BIOTECHNOLOGY,60(12):912-923 2018(Dec.) Author:Nakamura, Mikiko; Aihara, Junya; Hoshida, Hisashi; Akada, Rinji;
End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter MOLECULAR BIOTECHNOLOGY,57(11-12):1018-1029 2015(Dec.) Author:Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Tomiyoshi, Keisuke; Hoshida, Hisashi; Akada, Rinji;
A synthetic medium optimized for growth and a minimal medium for fermentation in yeasts YEAST,32:S228-S228 2015(Sep.) Author:Akada, Rinji; Misumi, Yukie; Nakagawa, Takaaki; Iwakiri, Ryo; Nakamura, Mikiko; Hoshida, Hisashi;
A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells MOLECULAR BIOTECHNOLOGY,57(8):767-780 2015(Aug.) Author:Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Yarimizu, Tohru; Iwakiri, Ryo; Hoshida, Hisashi; Akada, Rinji;
Screening of accurate clones for gene synthesis in yeast JOURNAL OF BIOSCIENCE AND BIOENGINEERING,119(3):251-259 2015(Mar.) Author:Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus MICROBIAL CELL FACTORIES,14 2015 Author:Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;
Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus YEAST,31(1):29-46 2014(Jan.) Author:Hoshida, Hisashi; Murakami, Nobutada; Suzuki, Ayako; Tamura, Ryoko; Asakawa, Jun; Abdel-Banat, Babiker M. A.; Nonklang, Sanom; Nakamura, Mikiko; Akada, Rinji;
Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae YEAST,30(12):485-500 2013(Dec.) Author:Yarimizu, Tohru; Nonklang, Sanom; Nakamura, Junpei; Tokuda, Shuya; Nakagawa, Takaaki; Lorreungsil, Sasithorn; Sutthikhumpha, Surasit; Pukahuta, Charida; Kitagawa, Takao; Nakamura, Mikiko; Cha-aim, Kamonchai; Limtong, Savitree; Hoshida, Hisashi; Akada, Rinji;
Novel Small-Molecule Compounds That Affect Cellular Morphogenesis in Yeast and Mammalian Cells BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,77(8):1669-1676 2013(Aug.) Author:Fukunaga, Tomoaki; Nakamura, Mikiko; Kitagawa, Takao; Watanapokasin, Ramida; Hoshida, Hisashi; Akada, Rinji;
Designed construction of recombinant DNA at the ura30 locus in the yeast Saccharomyces cerevisiae YEAST,30(6):243-253 2013(Jun.) Author:Fukunaga, Tomoaki; Cha-aim, Kamonchai; Hirakawa, Yuki; Sakai, Ryota; Kitagawa, Takao; Nakamura, Mikiko; Nonklang, Sanom; Hoshida, Hisashi; Akada, Rinji;
講演・口頭発表等 PCR-mediated expressions of a gene in triple hosts: yeast, E. coli, and mammalian cells PEPTALK2023 2023(Jan. 16) Presenter:Nakamura Mikiko
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