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中村美紀子  ナカムラ ミキコ

教員組織電話番号
教育組織基盤研究支援センターFAX番号
職名准教授(特定雇用)メールアドレス
住所〒390-8621 長野県松本市旭3-1-1ホームページURL

プロフィール

所属学会
所属学会
日本生物工学会、日本農芸化学会、日本分子生物学会、酵母遺伝学フォーラム
学歴
取得学位
博士(医工学) , 山口大学

研究活動業績

研究業績(著書・
発表論文等)
著書
続・生物工学基礎講座 バイオよもやま話 3番目のDNA連結反応 NHEJ: なんとほんとにeasyじゃん
日本生物工学会 , 生物工学 , 98(1):23-27 2020
Author:赤田倫治、中村美紀子、星田尚司


大容量PCR装置によるmLスケールでのDNA生産
シーエムシー出版 , Bio Industry , 36(9):58 2019
Author:赤田倫治、星田尚司、鈴木絢子、中村美紀子、藤本哲憲


第3章 耐熱性酵母Kluyveromyces marxianusを用いた物質生産と育成技術
シーエムシー出版 , 酵母菌・麹菌・乳酸菌の産業応用展開 Advances in industrial applications of Yeast, Koji-molds and Lactic Acid bacteria 2018(Jan. 30)
Author:赤田倫治、中村美紀子、星田尚司


Minimum GC-rich sequences for overlap extension PCR and primer annealing
Springer Nature , Methods in Molecular Biology. DNA cloning and Assembly methods , (1116):165-181 2014
Author:Nakamura Mikiko, Suzuki Ayako, Hoshida Hisashi, Akada Rinji


論文
YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-mu m circle sequence in cir(0) strains of Saccharomyces cerevisiae
YEAST,36(5):249-257 2019(May)
Author:Misumi, Yukie; Nishioka, Satoko; Fukuda, Akira; Uemura, Takeshi; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;


Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products
MOLECULAR BIOTECHNOLOGY,60(12):912-923 2018(Dec.)
Author:Nakamura, Mikiko; Aihara, Junya; Hoshida, Hisashi; Akada, Rinji;


End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter
MOLECULAR BIOTECHNOLOGY,57(11-12):1018-1029 2015(Dec.)
Author:Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Tomiyoshi, Keisuke; Hoshida, Hisashi; Akada, Rinji;


A synthetic medium optimized for growth and a minimal medium for fermentation in yeasts
YEAST,32:S228-S228 2015(Sep.)
Author:Akada, Rinji; Misumi, Yukie; Nakagawa, Takaaki; Iwakiri, Ryo; Nakamura, Mikiko; Hoshida, Hisashi;


A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells
MOLECULAR BIOTECHNOLOGY,57(8):767-780 2015(Aug.)
Author:Nakamura, Mikiko; Suzuki, Ayako; Akada, Junko; Yarimizu, Tohru; Iwakiri, Ryo; Hoshida, Hisashi; Akada, Rinji;


Screening of accurate clones for gene synthesis in yeast
JOURNAL OF BIOSCIENCE AND BIOENGINEERING,119(3):251-259 2015(Mar.)
Author:Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;


Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus
MICROBIAL CELL FACTORIES,14:- 2015
Author:Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi; Akada, Rinji;


Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus
YEAST,31(1):29-46 2014(Jan.)
Author:Hoshida, Hisashi; Murakami, Nobutada; Suzuki, Ayako; Tamura, Ryoko; Asakawa, Jun; Abdel-Banat, Babiker M. A.; Nonklang, Sanom; Nakamura, Mikiko; Akada, Rinji;


Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae
YEAST,30(12):485-500 2013(Dec.)
Author:Yarimizu, Tohru; Nonklang, Sanom; Nakamura, Junpei; Tokuda, Shuya; Nakagawa, Takaaki; Lorreungsil, Sasithorn; Sutthikhumpha, Surasit; Pukahuta, Charida; Kitagawa, Takao; Nakamura, Mikiko; Cha-aim, Kamonchai; Limtong, Savitree; Hoshida, Hisashi; Akada, Rinji;


Novel Small-Molecule Compounds That Affect Cellular Morphogenesis in Yeast and Mammalian Cells
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY,77(8):1669-1676 2013(Aug.)
Author:Fukunaga, Tomoaki; Nakamura, Mikiko; Kitagawa, Takao; Watanapokasin, Ramida; Hoshida, Hisashi; Akada, Rinji;


Designed construction of recombinant DNA at the ura30 locus in the yeast Saccharomyces cerevisiae
YEAST,30(6):243-253 2013(Jun.)
Author:Fukunaga, Tomoaki; Cha-aim, Kamonchai; Hirakawa, Yuki; Sakai, Ryota; Kitagawa, Takao; Nakamura, Mikiko; Nonklang, Sanom; Hoshida, Hisashi; Akada, Rinji;

特許等知的財産
登録済特許
哺乳動物細胞への遺伝子導入効率の向上剤
RNA発現用線状二本鎖DNA
変異RNA発現用線状二本鎖DNA
分泌シグナルペプチドをコードするDNA
研究費
科学研究費補助金(研究代表者)
2013 - 2015 , PCR合成DNAによる革新的な新規遺伝子発現抑制RNAi技術の開発 , 挑戦的萌芽研究