Doctor of Agriculture

昆虫分子生物学

1974, Shinshu University, Graduate School, Division of Textile Science and Technology

1979, Nagoya University, Graduate School, Division of Agriculture

1972, Shinshu University, Faculty of Textile Science and Technology

Secondary structure analysis of spider dragline silk fibers obtained by different spinning methods using Raman spectroscopy
ZHANG Lei, BAO Xian-xun, LIU Ming-hui, HAN Leng, Nakagaki Masao
Journal of Anhui Agricultural University, 40(1), 144-148, 2013, Refereed

The variability of mechanical properties and molecular conformation among different spider dragline fibers
Lei Zhang, Leng Han, Yujun Wang, Tianfu Zhao, Xianxun Bao, and Masao Nakagaki
Fibers and Polymers, 14(7), 1190-1195, 2013, Refereed

Expression analysis of spiderdragline silk gene MaSp1 in the transgenic silkworm
Han Leng, Zhang Lei, Nakagaki Masao
Journal of Anhui Agricultural University, 40(2), 169-174, 2013, Refereed

The molecular structures of major ampullate silk proteins of the wasp spider, Argiope bruennichi: A second blueprint for synthesizing de novo silk
Yang Zhang, Ai-Chun Zhao, Yang-Hu Sima, Cheng Lu, Zhong-Huai Xiang, Masao Nakagaki
Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology, 164(3), 151–158, 2013, Refereed

Intramolecular Homologous Recombination Event Occurred in the Spider Egg Case Silk Gene CySp2 of Wasp Spider
Leng Han and Masao Nakagaki
Molecular Biology, 47(5), 682–685, 2013, Refereed

Analysis of a new type of major ampullate spider silk gene, MaSp1s
Leng Han, Lei Zhang, Tianfu Zhao, Yujun Wang, Masao Nakagaki
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 56, 156–161, 2013, Refereed

Diversity in Copy Number and Structure of a Silkworm Morphogenetic. Gene as a Result of Domestication
Takashi Sakudoh, Takeharu Nakashima, Yoko Kuroki, Asao Fujiyama, Yuji Kohara, Naoko Honda, Hirofumi Fujimoto, Toru Shimada, Masao Nakagaki, Yutaka Banno and Kozo Tsuchida
Genetics, 187, 965-976, 2011

Characterization of a cysteine-rich protein specifically expressed in the silk gland of caddisfly Stenopsyche marmorata　(Trichoptera; Stenopsychidae),
Wang Y.J., Wang H., Zhao T.F., Nakagaki M.
Biosci. Biotech. Biochem., 74(1), 108-112, 2010

Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons.
Wen H, Lan X, Zhang Y, Zhao T, Wang Y, Kajiura Z, Nakagaki M.
Mol Biol Rep., 37(4), 1815-1821, 2010

Characterization of unique heavy chain fibroin filaments spun underwater by the caddisfly Stenopsyche marmorata (Trichoptera; Stenopsychidae).
Wang Y.J., Sanai K., Wen H.X., Zhao T.F., Nakagaki M.
Mol Biol Rep., 37(6), 2885-2892, 2010

New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm.
Zhao A, Zhao T, Zhang Y, Xia Q, Lu C, Zhou Z, Xiang Z, Nakagaki M.
Transgenic Res., 19(1), 29-44, 2010

A novel bioadhesive protein of silk filaments spun underwater by caddisfly larvae.
Wang Y.J., Sanai K., Nakagaki M.
Adv. Mat. Res., 79-82, 1631-1634, 2009

Developmental changes in D-serine level in the silkworm, Bombyx mori
Yuichiro Hasegawa, Minoru Okumura, Miki Tojo, Masao Nakagaki and Yoko Nagata
J. Insect Biotech. Sericology, 78(2), 69-73, 2009

Effects of O-phospho-L-serine on the larval growth and development in the silkworm, Bombyx mori
Yuichiro Hasegawa, Tomomi Ogawa, Mami Nakanishi, Miki Tojo, Minoru Okumura, Masao Nakagaki and Yoko Nagata
J. Insect Biotech. Sericology, 78(2), 75-79, 2009

Sequence structure and expression pattern of a novel anionic defensin-like gene from silkworm (Bombyx mori).
Hongxiu Wen, Xiqian Lan, Tingcai Cheng, Ningjia He, Kunihiro Shiomi, Zenta Kajiura ,Zeyang Zhou, Qingyou Xia, Zhonghuai Xiang ,Masao Nakagaki
Mol Biol Rep., 36(4), 711-716, 2009

Purification and cDNA Cloning of Vitellogenin of the Wild Silkworm, Saturnia japonica (Lepidoptera: Saturniidae)
Yan Meng, Chao Liang Liu, Kunihiro Shiomi, Masao Nakagaki and Zenta Kajiura
J. Insect Biotech. Sericology, 77(1), 35-44, 2008

Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield.
Yuansong Zhang, Junhua Hu, Yungen Miao, Aichun Zhao, Tianfu Zhao, Dayang Wu ,Liefeng Liang, Ayumi Miikura, Kunihiro Shiomi, Zenta Kajiura, Masao Nakagaki.
Mol Biol Rep., 35(3), 329-335, 2008

ジョロウグモ（Nephila clavata）牽引糸タンパク質MaSp1遺伝子のcDNAクローニングおよびカイコフィブロイン遺伝子との配列解析．
張 袁松, 清水 一彦, 塩見 邦博, 梶浦 善太,中垣 雅雄．
蚕糸・昆虫バイオテック, 77(1), 39-46, 2008

Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system.
Yue W, Miao Y, Li X, Wu X, Zhao A, Nakagaki M.
Appl Microbiol Biotechnol., 73, 181-186, 2007

The Pitx homeobox gene in Bombyx mori: Regulation of DH-PBAN neuropeptide hormone gene expression.
Shiomi K, Fujiwara Y, Yasukochi Y, Kajiura Z, Nakagaki M, Yaginuma T.
Mol Cell Neurosci., 34, 209-218, 2007

A covariant change of the two highly conserved bases in the GTPase-associated center of 28 S rRNA in silkworms and other moths
T. Uchiumi/T. Nomura/T. Shimizu/Y. Katakai/K. Mita/Y. Koik/ M. Nakagaki,
JOURNAL OF BIOLOGICAL CHEMISTRY, 275(45), 35116-35121, 2000

家蚕濃核病2型ウイルス（BmDNV-2）に感染したカイコの中腸被膜における2種類のウイルスDNAの量的変動
中垣雅雄
日本蚕糸学雑誌, 68, 173-180, 1999

消石灰液によるカイコ核多角体病ウイルス(NPV)の不活化について
周垂欽／中垣雅雄
日本蚕糸学雑誌, 68(3), 201-207, 1999

カイコ中腸におけるNPVの増殖とポリヘドリン遺伝子の発現
津田英利／中垣雅雄
日本蚕糸学雑誌, 68, 19-25, 1999

Determination of nucleotide sequence and transcription initiation site of 25K gene of Bombyx mori Nucleopolyhedrovirus
H. Tsuda/M. Nakagaki
J. Sericult. Sci. Jpn, 68(1), 27-39, 1999

カイコ絹糸腺リボソームの調製とその性質
野村隆臣／内海利男／中垣雅雄
日本蚕糸学雑誌, 67, 311-318, 1998

高度さらし粉水溶液で卵殻を除去した天蚕前幼虫に対するイミダゾール化合物KK-42の休眠覚醒効果
福本匡志／中垣雅雄
日本蚕糸学雑誌, 67, 65-71, 1998

Purification, developmental profile and biosynthesis of arylphorin in the wild silkmoth, Antheraea pernyi
Z. Kajiura/M. N. Yokoyama/M. Nakagaki
Applied Entomology and Zoology, 33(2), 305-313, 1998

幼若ホルモンおよびエクダイソンを処理した劣性３眠蚕における精細胞の出現時期の変更
何麗／中垣雅雄
日本蚕糸学雑誌, 66, 192-199, 1997

遺伝的３眠蚕における精子細胞形成
中垣雅雄
日本蚕糸学雑誌, 66, 17-22, 1997

応用生物学入門
中垣雅雄, コラム15. スパイダーシルクの開発
オーム社, 217-219 2010

機能性繊維の最新技術
中垣雅雄, 第3章 環境調和技術の開発と展望．5 クモの糸を吐く蚕
シーエムシー出版, 130-140 2009

Comparison of Mechanical Strength of Three Kinds of Spider's Drag-lines
Zhang Lei, Wang Yujun, Han Leng, Zhao Tianfu, Masao Nakagaki
International Conference Future Textile 57-58, Jul. 2010

Analysis of a New Kind of Major Ampullate Spider Silk Gene
Han Leng, Zhao Tianfu, Zhang Lei, Wang Yujun, Xia Qingyou, Xiang Zhonghuai, Masao Nakagaki
International Conference Future Textile 59-60, Jul. 2010

A novel bioadhesive protein of silk filaments spun underwater by caddisfly larvae
Y. Wang, M. Nakagaki
5th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda., 2009

Production of silkworm defensinA in Escherichia coli by soluble fusion expression
H. Wen, X. Lan, M. Nakagaki
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009), 2009

Compare the Light-chain fibroins of Trichoptera and Lepidopteron
Y. Wang, M. Nakagaki
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009), 2009

Molecular cloning and expression analysis of thermoTRP homologs of the silkworm, Bombyx mori
A. Sato, H. Uehara, Y. Fukushima, M. Hirabayashi, Z. Kajiura, M. Nakagaki, K. Shiomi
Asia-Pacific Congress of Sericulture and Insect Biotechnology 74, 2008

Polymorophic Analysis of silkworm vitellogenins
Zenta Kajiura，Yan Meng, Chaoliang Liu, Kunihiro Shiomi, Yutaka Banno and Masao Nakagaki
CISC-6, Sep. 2007

Analysis of an Anionic Defensin-Like Gene from Silkworm
H. Wen, X. Lan, K. Shiomi, Z. Kajiura, M. Nakagaki
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007), 2007

Study on expression of spider silk in silkworm (Bombyx mori)
H. Wen, X. Lan, T. Zhao, Y. Zhang, Y. Wang, K. Shiomi, Z. Kajiura, M. Nakagaki
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007), 2007

Studies on Silk Protein in the Tyichoptera (Stenopsychidae marmorata)
Y. Wang, S. Wozumi, H. Wen, K. Shiomi, Z. Kajiura, M. Nakagaki
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007), 2007

昆虫ウィルス病に関する疫学的及び分子生物学的研究