Doctor of Agriculture, Hokkaido Univ.

Applied biochemistry, Enzyme chemistry, Applied microbiology, Genetic engineering

2007
Part-time Lecturer, Hirosaki Univ.

2005
Associate Professor, Shinshu Univ.

1999 - 2004
Assistant Professor, Shinshu Univ.

1995, Hirosaki University, Graduate School, Division of Agriculture

1998, Hokkaido University, Graduate School, Division of Agriculture

1993, Hirosaki University, Faculty of Agriculture

Antimicrobial and mechanical properties of silver ion-exchanged transparent lithium-mica glass-ceramics.
Taruta S, Matsuyama K, Nozaki K
Ceramics International, 49, 15959-15968, 2023

Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor
T. Arai, P. Biely, I. Uhliariková, N. Sato, S. Makishima, M. Mizuno,K. Nozaki, S. Kaneko, Y. Amano
J Biosci Bioeng, 127(2), 2019, Refereed

Orientation control of Trametes laccases on a carbon electrode surface to understand the orientation effect on the electrocatalytic activity
Hoshikawa Y, Castro-Muñiz A, Tawata H, Nozaki K, Yamane S, Itoh T, Kyotani T
Bioconjug Chem, 2018, Refereed

Comparison of catalytic properties of multiple β-glucosidases of Trichoderma reesei
B. Guo, N. Sato, P. Biely, Y. Amano. K. Nozaki
Appl Microbiol Biotechnol, 08 Feb. 2016, Refereed
Corresponding

Improvements in glucose sensitivity and stability of Trichoderma reesei β-glucosidase using site-directed mutagenesis
B. Guo, Y. Amano, K. Nozaki
PLOS ONE, 18 Jan. 2016, Refereed

Trichoderma reesei におけるセルラーゼ誘導・生産メカニズムの解明 ーβ-グルコシダーゼの役割についてー（総説）
野﨑功一，郭 博洋，畠中理志，天野良彦
応用糖質科学, 6(2), 96-102, 2016

Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability
Nishijima, H; Nozaki, K; Mizuno, M; Arai, T; Amano, Y
Biosci Biotechnol Biochem, 79(5), 738-746, 04 May 2015

Irpex lacteus由来GH10キシラナーゼの新規セルロース高吸着CBM1（総説）
西島拓人，野﨑功一，水野正浩，荒井勉，天野良彦
応用糖質科学, 4(2), 154-159, 01 Apr. 2014

担子菌バイオマス分解系酵素群の機能解析（総説）
野﨑功一
応用糖質科学, 3(1), 61-67, 01 Apr. 2013

Production of feruloylated arabino-oligosaccharides (FA-AOs) from beet fiber by hydrothermal treatment
N. Sato, Y. Takano, M. Mizuno, K. Nozaki, S. Umemura, T. Matsuzawa, Y. Amano, S. Makishima
J Supercritical Fluids, 79, 84-91, 2013

Structure of regenerated celluloses treated with ionic liquid and comparison of their enzymatic digestibility by purified cellulose components.
M. Mizuno, S. Kachi, E. Togawa, N. Hayashi, K. Nozaki, T. Itoh, Y. Amano
Aust. J. Chem., 65, 1491-1496, 2012

Creation of bacterial cellulose-fabric complexed material
Mizuno, M; Kamiya, Y; Katsuta, T; Oshima, N; Nozaki, K; Amano, Y
Sen-i Gakkaishi, 68(2), 42-47, 2012

Ultrafine cellulose fibers produced by Asaia bogorensis, an acetic acid bacterium
Kumagai, A; Mizuno, M; Kato, N; Nozaki, K; Togawa, E; Yamanaka, S; Okuda, K; Inder M. Saxena, Amano, Y
Biomacromol, 12(7), 2815-2821, 2011

Comparative study on the ability to produce gentiobiose in cellulose-producing bacteria Asaia bogorensis and Gluconacetobacter xylinus
Kumagai, A; Mizuno, M; Nozaki, K; Saxena, I M; Amano, Y
J Appl Glycosci, 58(4), 147-150, 2011

Regulation of adsorption behavior of carbohydrate-binding domain family 1 and endo-β-1,4-glucanase onto crystalline cellulose.
Nozaki, K; Nishijima, H; Arai, T; Mizuno, M; Sato, N; Amano, Y
J Appl Glycosci, 58(4), 133-138, 2011

Enzymatic production of glucosylxylose using a cellobiose phosphorylase-yeast combined system
Kumagai, A; Tada, S; Nozaki, K; Mizuno, M; Kanda, T; Suzuki, S; Kusumoto, K; Sasaki, T; Kashiwagi, Y; Amano, Y
J Appl Glycosci, 58, 31-34, 2011

Improvement in the productivity of xylooligosaccharides from waste medium after mushroom cultivation by hydrothermal treatment with suitable pretreatment
Sato, N; Shinji, K; Mizuno, M; Nozaki, K; Suzuki, M; Makishima, S; Shiroishi, M; Onoda, T; Takahashi, F; Kanda, T; Amano, Y
Bioresour Technol, 101(15), 6006-6011, Aug. 2010

Development of continuous flow type hydrothermal reactor for hemicellulose fraction recovery from corncob
S. Makishima, M. Mizuno, N. Sato, K. Shinji, M. Suzuki, K. Nozaki, F. Takahashi, T. Kanda, Y. Amano
Bioresour Technol, 100(1), 2842-2848, 2009, Refereed

Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression in Pichia pastoris
H. Toda, N. Nagahata, Y. Amano, K. Nozaki, T. Kanda, M. Okazaki, M. Shimosaka
Biosci Biotechnol Biochem, 72(12), 3142-3147, 2008, Refereed

Screening and investigation of dye decolorization activity produced by basidiomycetes
Nozaki, K; Beh, CH; Mizuno, M; Isobe, T; Shiroishi, M; Kanda, T; Amano, Y
J Biosci Bioeng, 2008, Refereed

Structure and characteristics of an endo-beta-1,4-glucanase, isolated from Trametes hirsuta, with high degradation to crystalline cellulose
Nozaki, K; Seki, T; Matsui, K; Mizuno, M; Kanda, T; Amano, Y
Biosci Biotechnol Biochem, 71(10), 2375-2382, 2007, Refereed

Structural analysis of the water-soluble carbohydrate from Asaia bogorensis by NMR spectroscopy
Kato, N; Mizuno, M; Nakai, Y; Nozaki, K; Suga, H; Kanda, T; Yamanaka, S; Amano, Y
J Appl Glycosci, 54, 231-233, 2007, Refereed

Viability and cellulose synthesizing ability of Gluconacetobacter xylinus cells under high-hydrostatic pressure.
Kato, N; Sato, T; Kato, C; Yajima, M; Sugiyama, J; Kanda, T; Mizuno, M; Nozaki, K; Yamanaka, S; Amano, Y
Extremophiles, 11, 693-698, 2007, Refereed

Recovery of soluble sugars from waste medium for Enokitake (Flammulina velutipes) mushroom cultivation with hydrothermal reaction and enzyme digestion
Makishima, S; Nozaki, K; Mizuno, M; Netsu, E; Shinji, K; Shibayama, T; Kanda, T; Amano, Y
J App Glycosci, 53, 261-266, 2006, Refereed

Effects of culture medium components on cultivation of Flammulina velutipes and characteristics of fruit body
Shiroishi, M; Nishizawa, K; Nozaki, K; Amano, Y; Kanda, T
J Appl Glycosci, 52, 133-136, 2005, Refereed

Accumulation of cello-oligosaccharides during bacterial cellulose production by Acetobacter xylinum
Ito, F; Amano, Y; Shiroishi, M; Nozaki, K; Saxena, IM; R Malcolm Brown Jr.; Kanda, T
J Appl Glycosci, 52, 27-30, 2005, Refereed

Morphological observation of partially carboxymethylated cotton treated by cellulase
Yamada, M; Amano, Y; Nozaki, K; Kanda, T
Sen-i Gakkaishi, 61(2), 48-54, 2005, Refereed

Action of cellulase on partially modified cotton by carboxymethylation
Yamada, M; Amano, Y; Horikawa, E; Izawa, Y; Nozaki, K; Kanda, T
Sen-i Gakkaishi, 61(1), 13-18, 2005, Refereed

Mode of action of cellulases on dyed cotton with a reactive dye
Yamada, M; Amano, Y; Horikawa, E; Nozaki, K; Kanda, T
Biosci Biotechnol Biochem, 69(1), 45-50, 2005, Refereed

The relationship between cellulase activity, oligosaccharides and cellulose production by Acetobacter xylinum ATCC23769
Ito, F; Amano, Y; Nozaki, K; Saxena, IM;RM Brown Jr.; Kanda, T
J Biol Macromol, 4, 83-90, 2004, Refereed

Transglycosylation reaction and acceptor specificity of exo- and endo-type cellulases
Nozaki, K; Kano, A; Amano, Y; Murata, T; Usui, T; Ito, K; Kanda, T
J Appl Glycosci, 51, 87-92, 2004, Refereed

Hydrolysis of water-soluble and water-insoluble cellulosic substrates by endo-beta-1,4-glucanase from Acetobacter xylinum
Ito, F; Amano, Y; Nozaki, K; Saxena, IM; R. M. Brown Jr.; Kanda,T
J Appl Glycosci, 51, 297-301, 2004, Refereed

Analysis of cellulase action using bacterial cellulose with unique structure.
Amano, Y; Nozaki, K; Kanda, T; Bowling, AJ; Saxena, IM; Brown, RM
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 227, U306-U306, 2004, Refereed

Starch properties of the sago palm (Metroxylon sagu Rottb.) in different soils
Nozaki, K; Nuyim, T; Shinano, T; Hamada, S; Ito, H; Matsui, H; Osaki, M
Plant Foods Hum Nutr, 59(3), 85-92, 2004, Refereed

Cloning and characterization of endo-type cellulase gene from Coriolus hirsutus
Matsui, K; Seki, T; Nozaki, K; Amano, Y; Kanda, T
Proceedings of MIE BIOFORUM 2003, 576-578, 2003

New insight on cellulose degradation by cellulase from fungi
Amano, Y; Nozaki, K; Kanda, T
Proceedings ofMIE BIOFORUM 2003, 509-516, 2003

The function of endo-beta-1,4-glucanase from Acetobacter xylinum during cellulose biosynthesis
Ito, F; Amano, Y; Nozaki, K; Kanda, T; Saxena, IM; Brown Jr., MR
Proceedings of MIE BIOFORUM 2003, 875-883, 2003

Molecular cloning of a cDNA encoding granule-bound starch synthase I from kidney bean seeds and expression in Esherichia coli
Isono, N; Ito, H; Senoura, T; Yoshikawa, H; Nozaki, K; Matsui, H
J Appl Glycosci, 50, 355-360, 2003, Refereed

Structure and physicochemical properties ofstarches from kidney bean seeds at immature, premature and mature stages of development
Yoshida, H; Nozaki, K; Hanashiro, I; Yagi, F; Ito, H; Honma, M; Matsui, H; Takeda, Y
Carbohydr Res, 338, 463-469, 2003, Refereed

Some characteristics of three groups in Flammulina velutipes classified by analysis of esterase isozymes
Nisizawa, K; Amano, Y; Nozaki, K; Hosokawa, N; Shiroishi, M; Kanda, T
Mycosci, 44, 19-23, 2003, Refereed

Synergistic effects of cellobiose dehydrogenase, cellulases, and beta-glucosidase from Irpex lacteus in the degradation of various type of cellulose
Hai, PQ; Nozaki, K; Amano, Y; Kanda, T
J Appl Glycosci, 49, 9-17, 2002, Refereed

Progress on studying the biological role of cellobiose dehydrogenase from Irpex lacteus
Hai, PQ; Nozaki, K; Amano, Y; Kanda, T
J Biol Macromol, 2, 11-18, 2002, Refereed

Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L..
Hamada, S; Ito, H; Hiraga, S; Inagaki, K; Nozaki, K; Isono, N; Yoshimoto, Y; Takeda, Y; Matsui, H
J Biol Chem, 277, 16538-16546, 2002, Refereed

Aglycone specificity in transglycosylation of a xylanase produced from Basidiomycete, Hypsizigus marmoreus during the mushroom cultivation
Nishizawa,K; Amano, Y; Isobe, T; Nozaki, K; Shiroishi, M; Kanda, T
J Appl Glycosci, 49, 137-143, 2002, Refereed

Peroxidase assay in mycelia of Flammulina velutipes for screening undesirable changes inpinning
Nishizawa, K; Amano, Y; Shiroishi, M; Suzuki, I; Nozaki, K; Miyashita, K; Kanda, T
Mokuzai Gakkaishi, 48(1), 38-43, 2002, Refereed

Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)
Nozaki, K; Isono, N; Hamada, S; Sagisaka, S; Hiraga, S; Ito, H; Matsui, H
J Grad School Agr Hokkaido Univ, 70(2), 89-109, 2001

Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)
Nozaki, K; Hamada, S; Nakamori, T; Ito, H; Sagisaka, S; Yoshida, H;Takeda, Y; Honma, M; Matsui, H
Biosci Biotechnol Biochem, 65, 1141-1148, 2001, Refereed

Two starch branching enzyme isoforms occur in different fractions of developing seeds of kidney bean
Hamada, S; Nozaki, K; Ito, H; Yoshimoto, Y; Yoshida, H; Hiraga, S; Onodera, S; Honma, M; Takeda, Y; Matsui, H
Biochem J, 359, 23-34, 2001, Refereed

Analysis of genetic differences among the strains of Flammulina veltipes (Enokitake)
Nishizawa, K; Amano, Y; Nozaki, K; Shiroishi, M; Hosokawa, N; Miyashita, K; Kanda, T
J Appl Glycosci, 48, 215-221, 2001, Refereed

Reactivities of cellulases from fungi towards ribbon-type bacterial cellulose and band-shaped bacterial cellulose
Amano, Y; Nozaki, K; Araki, T; Shibasaki, H; Kuga, S; Kanda, T
Cellulose, 8(4), 267-274, 2001, Refereed

Purification and characterization of cellobiose dehydrodenase from Irpex lacteus and its adsorption on cellulose
Hai, PQ; Nozaki, K; Amano, Y; Kanda, T
J Appl Glycosci, 47, 311-318, 2000, Refereed

Trafficking of starch synthase from the cytosol to functional sites in younger and older proplastidsin developing stolons of potato
Sagisaka, S; Akita, T; Akase, T; Matsumoto, K; Nozaki, K; Matsui, H; Ito, H; Honma, M
J Plant Physiol, 154, 310-318, 1999, Refereed

Amylases and branching enzyme of developing kidney bean seeds on native electrophoretic gel
Nozaki, K; Matsui, H; Tonokawa, T; Mori, H; Ito, H; Honma, M; Chiba, S
J Appl Glycosci, 45, 117-122, 1998, Refereed

Novel exopolygalacturonases produced by Alternaria mali
Nozaki, K; Miyairi, K; Hozumi, S; Fukui, Y; Okuno, T
Biosci Biotechnol Biochem, 61, 75-80, 1997, Refereed

バイオマス分解酵素の改良と利用
野﨑功一
技報こまくさ, 12, 19 - 20, 2014

セルラーゼの酵素反応を促進する新規タンパク質（スオレニン）の機能解析
野﨑功一
技報こまくさ, 11, 6-9, 01 Nov. 2013

リグニンを分解する第３のペルオキシダーゼ（解説）
野﨑功一
化学と生物, 40, 180 - 181, 2002

Insight in the biological role of cellobiose dehydrogenase (Review article)
Hai, PQ, Amano, Y, Nozaki, K, Kanda, T
J Biol Macromol, 1, 37 - 43, 2001, Refereed

Branching enzyme（解説）
Nozaki, K, Hamada, S
Glycoforum, 2000

生体触媒“酵素”の謎に迫る
野﨑功一
経済月報，(財)長野経済研究所, 2022

特集：革新的環境技術の創製ー糸状菌バイオマス分解酵素の生産能力の増強技術
野﨑功一
ケミカルエンジニヤリング，化学工業社, 2018

バイオマス分解酵素研究の最前線ーセルラーゼ・ヘミセルラーゼを中心としてー　第１章 5. 担子菌 (Irpex lacteus) のセルラーゼ
天野良彦，野﨑功一
シーエムシー出版, 2012

Protein observation by giant carbon nano test tubes
H. Sayama, T. Ogura, Y. Tanaka, K. Nozaki, T. Itoh, H. Nishihara
第33回日本MRS年次大会, 14 Nov. 2023

LPMO9Aによるセルロースの酵素分解を促進する新規タンパク質Cip1の機能解析
岩永光喜，野﨑功一
令和５年度日本応用糖質科学会東日本支部シンポジウム, 14 Oct. 2023

Aspergillus oryzae 由来エンド-1,6-β-グルカナーゼ（AoBG5A）の発現系構築とβ-グルカンへの反応性
谷 悠太，國吉優斗，野﨑功一
令和５年度日本応用糖質科学会東日本支部シンポジウム, 14 Oct. 2023

Trichoderma reesei 由来β-グルコシダーゼ（TrCel3A）のアミノ酸変異によるグルコース耐性の改良
田中大幹，野﨑功一
日本応用糖質科学会2023年度大会, 13 Sep. 2023

麹菌クチナーゼアイソザイムの生分解性プラスチックに対する反応性の比較
三宅陽介，野﨑功一
日本農芸化学会2023年度大会, 16 Mar. 2023

糸状菌Trichoderma reesei 由来クチナーゼのアミノ酸変異による反応特性の改良
島 帆花，野﨑功一
日本生物工学会2022年度大会, 19 Oct. 2022

Trichoderma reesei 由来エンド-1,6-β-グルカナーゼの発現系構築とβ-グルカンへの作用特性
國吉優斗，野﨑功一
日本応用糖質科学会2022年度大会, 31 Aug. 2022

Trichoderma reesei 由来GH55エキソ-1,3-β-グルカナーゼの発現系構築と酵素化学的性質の調査
梶 暉，野﨑功一
日本応用糖質科学会2022年度大会, 31 Aug. 2022

セルロースで誘導発現する機能未知タンパク質Cip1の遺伝子破壊株の作製と解析
細川由芙子，野﨑功一
日本応用糖質科学会2021年度大会, 02 Sep. 2021

α-ガラクトシダーゼのアミノ酸変異によるガラクトマンナン側鎖ガラクトース残基の分解性の改良
俊成勇紀，野﨑功一
日本応用糖質科学会2021年度大会, 02 Sep. 2021

耐熱性および熱活性化を示すクチナーゼの性質
田口七海，野﨑功一
日本農芸化学会2021年度大会, 10 Mar. 2021

物質化学概論（分担）

The Japanese Society of Applied Glycoscience

Japan Society for Bioscience, Biotechnology, and Agrochemistry

The Cellulose Society of Japan

The Society for Biotechnology, Japan

糸状菌由来β-glucosidase活性を有する酵素の異種発現とパルプ糖化性評価
技術指導
2022 - 2023

生分解性フィルムの酵素分解に関する研究
共同研究
2020 - 2024

農工連携によるオリゴタンニンの高度利用に向けた基盤研究
信州大学URAファンド（分担）
2018

担子菌キノコの潜在能力追求による新規バイオ燃料製造プロセスの開発
信州大学グリーンイノベーション研究支援事業（分担）
2012

酒粕の有効利用を目指した機能性成分抽出法の開発
上田市・新技術助成金
2011

あんずの有効利用に関する共同研究
共同研究
2010 - 2011

産業用インクジェットプリンタ周辺装置の高機能化
ものづくり中小企業製品開発等支援助成金（経済産業省）
2009 - 2010

Investigation and Characterization of Biomass Degradation Enzymes

Study on Ligninolytic Enzymes from Fungi

Induction mechanism of cellulolytic enzymes in filamentous fungi

Study on Biomass Utilization