Sakurai Takayuki
Academic Assembly School of Medicine and Health Sciences Institute of Medicine
Interdisciplinary Cluster for Cutting Edge Research Institute for Biomedical Sciences
Associate Professor
Researcher Information
Research Keyword
- Developmental engineering, Genome Editing, Developmental biology, Transgenic animal
Mail Address
- tsakurai★shinshu-u.ac.jp
Career
Research activity information
Paper
- An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility
Negishi K, Aizawa K, Shindo T, Suzuki T, Sakurai T, Saito Y, Miyakawa Y, Tanokura M, Kataoka Y, Maeda M, Shota Tomida, Hiroyuki Morita, Takeda N, Komuro I, Kario K, Nagai R, Imai Y
Sci Rep, 12(1), 8844, 2022 - Receptor activity modifying protein RAMP sub-isoforms and their functional differentiation, which regulates functional diversity of adrenomedullin
Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Sakurai T
Biology, 11(5), 788, 2022 - Adrenomedullin-RAMP2 system ameliorates subretinal fibrosis by suppressing epithelial-mesenchymal transition in age-related macular degeneration
Tanaka M, Kakihara S, Hirabayashi K, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Cui N, Wei Y, Zhao Y, Aruga K, Yamauchi A, Murata T, Shindo T
Am J Pathol, 191(4), 652-668, 2021 - Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress
Cui N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Tanaka M, Wei Y, Kakihara S, Zhao Y, Aruga K, Kawagishi H, Nakada T, Yamada M, Shindo T
Endocrinology, 162(3), 1-20, 2021 - Adrenomedullin Ameliorates Pulmonary Fibrosis by Regulating TGF-ß-Smads Signaling and Myofibroblast Differentiation
Wei Y, Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Cui N, Kakihara S, Zhao, Y, Aruga K, Sanjo H, Shindo T
Endocrinology, 162(8), 1-22, 2021 - Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing
Sakurai T, Shindo T
STAR Protoc, 2(2), 2021 - Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
Sakurai, T.; Kamiyoshi, A.; Kawate, H.; Watanabe, S.; Sato, M.; Shindo, T.
Sci.Rep, 10(1), 1091, 2020 - Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts.
Dai, K.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Matsui, S.; Shindo, T.
Oncogene, 39(10), 1914-1930, 2020 - Development of a Novel Model of Central Retinal Vascular Occlusion and the Therapeutic Potential of the Adrenomedullin-Receptor Activity-Modifying Protein 2 System.
Hirabayashi, K.; Tanaka, M.; Imai, A.; Toriyama, Y.; Iesato, Y.; Sakurai, T.; Kamiyoshi, A.; Ichikawa-Shindo, Y.; Kawate, H.; Tanaka, M.; Dai, K.; Cui, N.; Wei, Y.; Nakamura, K.; Iida, S.; Matsui, S.; Yamauchi, A.; Murata, T.; Shindo, T.
Am J Pathol.,, 189, 449-466, 2019 - Endogenous Calcitonin Gene-Related Peptide Deficiency Exacerbates Postoperative Lymphedema by Suppressing Lymphatic Capillary Formation and M2 Macrophage Accumulation.
Matsui, S.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Dai, K.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Nakamura, K.; Yamauchi, A.; Ishida, K.; Tanaka, S.; Kawamata, M.; Shindo, T.
Am J Pathol.,, 189, 2487-2502, 2019 - Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice.
Yamauchi, A.; Kamiyoshi, A.; Sakurai, T.; Miyazaki, H.; Hirano, E.; Lim, HS.; Kaku, T.; Shindo, T.
Heliyon, 5, e02655, 2019 - Regulation of cardiovascular development and homeostasis by the adrenomedullin-RAMP system
Shindo T, Tanaka M, Kamiyoshi, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Sakurai T
PEPTIDES, 111, 55-61, 2019 - Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.
Sci Rep, 9(1), 9923, 2019 - Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid-gestational murine fetuses.
Nakamura S, Ishihara M, Ando N, Watanabe S, Sakurai T, Sato M
IUBMB Life, 71(7), 835-844, 2019 - RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders
Liu T, Kamiyoshi A, Tanaka M, Iida S, Sakurai T, Ichikawa-Shindo Y, Kawate H, Hirabayashi K, Dai K, Cui N, Tanaka M, Wei Y, Nakamura K, Matsui S, Yamauchi A, Shindo T
PEPTIDES, 110, 10-18, 2018 - In vivo genome editing targeted towards the female reproductive system.
Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Gurumurthy CB
Arch Pharm Res., 41(9), 898-910, 2018 - The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.
Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M.
Int J Mol Sci., 19(4), E1075, 2018 - Endogenous calcitonin gene-related peptide suppresses ischemic brain injuries and progression of cognitive decline.
Zhai L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Xian X, Hirabayashi K, Dai K, Cui N, Tanimura K, Liu T, Wei Y, Tanaka M, Tomiyama H, Yamauchi A, Igarashi K, Shindo T.
J Hypertens, 36(4), 876-891, 2018 - Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.
Theriogenology, 108, 29-38, 2018
- Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy.
Imai A, Toriyama Y, Iesato Y, Hirabayashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Liu T, Xian X, Zhai L, Dai K, Tanimura K, Liu T, Cui N, Yamauchi A, Murata T, Shindo T.
Am J Pathol., 187(5), 999-1015, 2017 - Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
Endocrinology., 158(5), 1359-1372, 2017 - Endogenous Calcitonin Gene-Related Peptide Regulates Lipid Metabolism and Energy Homeostasis in Male Mice.
Liu T, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Yang L, Tanaka M, Xian X, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.
Endocrinology., 158(5), 1194-1206, 2017 - Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.
Int J Mol Sci., 18(12), E2610, 2017 - Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K
Int.J.Mol.Sci, 16, 17838-17856, 2016 - The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis
Tanaka M, Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Liu T, Xian X, Imai A, Zhai L, Hirabayashi K, Owa S, Yamauchi A, Igarashi K, Taniguchi S, Shindo T
Cardiovasc.Res, 111, 398-409, 2016 - The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K
17, E1424, 2016 - Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury
Ayuzawa N, Nagase M, Ueda K, Nishimoto M, Kawarazaki W, Marumo T, Aiba A, Sakurai T, Shindo T, Fujita T
Hypertension, 67, 99-106, 2016 - Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques
Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K
Sci.Rep, 6, srep35861, 2016 - A non-inheritable maternal Cas9-based multiple-gene editing system in mice
Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T
Sci.Rep, 6, srep20011, 2016 - Pathophysiological Function of Endogenous Calcitonin Gene-Related Peptide in Ocular Vascular Diseases
Toriyama Y, Iesato Y, Imai A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Igarashi K, Tanaka M, Liu T, Xian X, Zhai L, Owa S, Murata T, Shindo T
Am J Pathol, 185, 1783-1794, 2015 - Adrenomedullin-RAMP2 System in Vascular Endothelial Cells
Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Shindo T
J Atheroscler Thromb, 22, 647-653, 2015 - A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S
Biotechnol J, 10, 143-153, 2015 - Generation of α-1,3-Galactosyltransferase-Deficient Porcine Embryonic Fibroblasts by CRISPR/Cas9-Mediated Knock-in of a Small Mutated Sequence and a Targeted Toxin-Based Selection System
Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S.
Reprod Domest Anim, 50, 872-880, 2015 - Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T
PLOS ONE, 9, e87667, 2014 - Functional differentiation of RAMP2 and RAMP3 in their regulation of vascular system
Yamauchi A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Igarashi K, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T
J Mol Cell Cardiol, 77, 73-85, 2014 - A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T
BMC Biotechnol, 14, 69, 2014 - Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia
Igarashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T
PEPTIDES, 62, 21-31, 2014 - The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
Xenotransplantation, 21, 291-300, 2014 - Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T
PLOS ONE, 9(2), e87667, 2014 - The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
Xenotransplantation, 21, 291-300, 2014 - Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
Biotechnol J, 8, 1355-1361, 2014 - Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression
Sato M Akasaka E Saitoh I Ohtsuka M Nakamura S Sakurai T, Watanabe S
Biology, 2, 341-355, 2013 - Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury
Yang L, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T..
J Mol Cell Cardiol., 59, 55-66, 2013 - Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study
Sato M Kubota N Inada E Saitoh I Ohtsuka M Nakamura S Sakurai T Watanabe S
Advances in Stem Cells, 2013(2013), ID 208514, 2013 - Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
Biotechnol J., 8(11), 1355-1361, 2013 - Regulation of adrenomedullin and its family peptide by RAMP system--lessons from genetically engineered mice
Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Shimoyama N, Iinuma N, Arai T, Miyagawa S.
Curr Protein Pept Sci., 14(5), 347-357, 2013 - Adrenomedullin-RAMP2 system is crucially involved in retinal angiogenesis.
Iesato Y, Toriyama Y, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Igarashi K, Murata T, Shindo T
Am J Pathol., 182(6), 2380-2390, 2013 - Novel Regulation of Cardiac Metabolism and Homeostasis by the Adrenomedullin-Receptor Activity-Modifying Protein 2 System
Yoshizawa T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Nakada T, Kashihara T, Yamada M, Kawakami H, Nakanishi H, Taguchi R, Nakanishi T, Akazawa H, Shindo T.
Hypertension, 61(2), 341-351, 2013 - Vascular Endothelial Adrenomedullin-RAMP2 System Is Essential for Vascular Integrity and Organ Homeostasis
Koyama T, Ochoa-Callejero L, Sakurai T ,Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Arai T, Yoshizawa T, Iesato Y, Lei Y, Uetake R, Okimura A, Yamauchi A, Tanaka M, Igarashi K, Toriyama Y, Kawate H, Adams RH, Kawakami H, Mochizuki N, Martínez A, Shindo T
Circulation., 127(7), 842-853, 2013 - Regulation of Adrenomedullin and its Family Peptide by RAMP System - Lessons from Genetically Engineered Mice
Shindo, T; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Shimoyama, N; Iinuma, N; Arai, T; Miyagawa, S
CURRENT PROTEIN & PEPTIDE SCIENCE, 14(5), 347-357, 2013 - A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates
Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S
J. Biomedical Science and Engineering, 5, 406-408, 2012 - A novel glycosylation signal regulates transforming growth factor b receptors as evidenced by endo-b-galactosidase C expression in rodent cells
Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Sato M
GLYCOBIOLOGY, 21, 486-492, 2011 - Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression
Watanabe S; Nakamura S; Sakurai T; Akasaka K; Sato M
N Biotechnol, 28, 312-319, 2011 - Induction of LYVE-1/stabilin-2 -positive liver sinusoidal endothelial-like cells from embryoid bodies by modulation of adrenomedullin-RAMP2 signaling.
Arai T, Sakurai T, Kamiyoshi A, Shindo Y, Iinuma N, Iesato Y, Koyama T, Yoshizawa T, Uetake R, Yamauchi A, Yang L, Kawate H, Ogawa S, Kobayashi A, Miyagawa S, Shindo T
PEPTIDES, 32, 1855-1865, 2011 - Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver
Iinuma, N; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Arai, T; Yoshizawa, T; Koyama, T; Uetake, R; Kawate, H; Muto, S; Tagawa, Y Miyagawa, S; Shindo, T
PEPTIDES, 31(5), 865-871, 2010 - Endogenous alpha-calcitonin gene-related peptide mitigates liver fibrosis in chronic hepatitis induced by repeated administration of concanavalin A.
Kamiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Iinuma N; Kawate, H; Yoshizawa T; Koyama T; Muto, S; Shindo, T
Liver Int, 29(5), 642-649, 2009 - Development of CRTEIL and CETRIZ, Cre-loxP-based system, whichi allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
Ohtsuka M; Warita T; Sakurai T; Watanabe S; Inoko H; Sato M
J Biomed Biotechnol, 2009(2009), 985140, 2009 - Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution
Sakurai T; Kamiyoshi A; Watanabe S; Sato M: Shindo T
Transgenic Res, 17(1), 149-155, 2008 - The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity.
Ichikawa-Shindo Y; Sakurai T; Kamiyoshi A; Kawate H et al.,
J Clin Invest, 118(1), 29-39, 2008 - Production and characterization of transgenic mice systemically expressing endo-beta-galactosidase C.
Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Yokomine TA; Sato M
GLYCOBIOLOGY, 18(1), 9-19, 2008 - Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
Nakanishi T; Kumagai S; Kimura M; Watanabe H; Sakurai T; Kimura M;Kashiwabara S; Baba T
Biochemical and biophysical research communications, 364(1), 14-19, 2007 - Preparationof enzymatically active human Myc tagged NCre recombinase exhibiting immunoreactivity with anti Myc antibody
Watanabe S; Honnma D; Furusawa T; Sakurai T; Sato M
Mol Reprod Dev, 73(11), 1345-1352, 2006 - Strain difference in tolerance to low-temperature treatment of fertilized mouse oocytes.
Sakurai T; Watanabe S; Sato M: Kimura M
Reprod Med Bio, 5(1), 43-50, 2006 - Folliculogenesis following syngeneic transplantation of young murine ovaries into the testes.
Sato M: Sakurai T; Kiryu K; Takeda M; Yasuoka Y
Reprod Med Bio, 5(1), 71-77, 2006 - Endogenous alpha CGRP protects against concanavalin A-induced hepatitis in mice
Kainiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Fukuchi, J; Kawate, H; Muto, S; Tagawa, Y; Shindo, T
Biochemical and biophysical research communications, 343(1), 152-158, 2006 - Azelnidipine, a long-acting dihydropyridine calcium channel blocker, suppresses the development of cardiac hypertrophy and fibrosis in adrenomedullin knock out mice.
Shindo, T; Niu, P; Kamiyoshi, A; Sakurai, T; Ichikawa, Y; Iinuma, N; Kawate, H
Circulation, 114(18), 13-13, 2006 - Testatin transgenic and knockout mice exhibit normal sex-differentiation
Hasegawa, K; Chuma, S; Tada, T; Sakurai, T; Tamura, M; Suemori, H; Nakatsuji, N
Biochemical and biophysical research communications, 341(2), 369-375, 2006 - A novel method for constructing murine cDNA library enriched with maternal mRNAs exhibiting de novo independent post-fertilization polyadenylation
Sakurai T; Sato M: Kimura M
Biochemical and biophysical research communications, 327(3), 688-699, 2005 - Temporal developmental arrest after storage of fertilized mouse eggs at 4oC: Effects on embryonic development, maternal mRNA processing and cell cycle
Sakurai T; Sato M: Kimura M
MORECULAR HUMAN REPRODUCTION, 11(5), 325-333, 2005 - Divers patterns of poiy(A) tail elongation and shortning of murinematernal mRNAs from fully-grown oocyte to 2-cell embryo stages
Sakurai T; Sato M: Kimura M
Biochemical and biophysical research communications, 336(4), 1181-1189, 2005, Refereed
Books and other publications
- Microinjection: Methods and Protocols Methods in Molecular Biology
Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B Gurumurthy, Masahiro Sato, Takayuki Shindo, Joint work, Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line
Springer Nature, Chapter 15 2019, RefereedResults and Problems in Cell Differentiation 63
Takayuki Sakurai, Takayuki Shindo, Masahiro Sato, Joint work, Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals
Springer, Chapter 21 2017, Refereed細胞工学 35 1
新藤隆行、桜井敬之、神吉昭子、市川優佳、田中愛、小山晃英, アドレノメデュリン-RAMP2シグナル
33-38 2016医学のあゆみ 223 13
新藤隆行 桜井敬之, アドレノメデュリンによる血管形成の調節-遺伝子操作動物における検討ー
医歯薬出版, 1063-1068 2007RNAi A GUIDE TO GENE SILENCING
桜井敬之 木村穣 (訳）, マウスの卵母細胞と着床前胚に対するRNAi実験入門
メディカルサイエンスインターナショナル, 303-333 2004Courses
Affiliated academic society
Research Themes
- 信州医学振興会
2023 - 2023 - 受容体活性調節システムによる生体内恒常性制御機構の解明と創薬への展開
科学研究費補助金, 基盤研究（B）
2021 - 2023 - アイバワークス医学研究者助成
2021 - 2021 - 血管およびリンパ管の恒常性制御は、癌転移抑制法となりうるか？
科学研究費補助金, 挑戦的研究(萌芽)
2020 - 2020 - 体系的複数同時ゲノム編集法を応用したRAMP1、2、3の機能分化と相互作用の解明
科学研究費補助金, 基盤研究 (C)
2019 - 2021 - 癌特異的代謝を介し増悪化に寄与するGPCR OR7C1の作用機序解明と治療応用
科学研究費補助金, 基盤研究(C)
01 Apr. 2018 - 31 Mar. 2021 - 信州医学振興会
2017 - 2017 - 「母性Cas9」による多遺伝子編集マウス開発と多因子性疾患の解明
科学研究費補助金, 萌芽研究
2016 - 2017 - 生理活性因子の情報制御システムに基づく、革新的な医薬品の創出, AMED-CREST
受託研究
2015 - 2016 - 創薬研究の加速に向けた、次世代遺伝子編集技術の開発 JST研究成果展開事業 マッチングプランナープログラム｢探索試験｣
科学研究費補助金
2015 - 2015 - 次世代遺伝子改変技術を応用した炎症性腸疾患におけるR2・R3の機能連関の解明
科学研究費補助金, 基盤研究（B）
2014 - 2017 - 臓器間連携と恒常性を司る、新しい生体内情報制御システムの解明と応用展開
科学研究費補助金, 基盤研究（B）
2014 - 2017 - 体系的・多遺伝子同時改変マウス作製法開発によるアドレノメデュリンRAMP機能解明
科学研究費補助金, 萌芽研究
2014 - 2016 - 受容体活性調節タンパクＲＡＭＰを標的とした創薬 科学技術振興機構 研究成果最適展開支援プログラム
受託研究
2013 - 2014 - アドレノメデュリン-RAMP2・RAMP3システムの破綻が惹起する炎症病態の解明
科学研究費補助金, 基盤研究（Ｃ）
2011 - 2014 - 内藤記念科学奨励金 血管統合機構の解明と治療応用
2011 - 2011 - 創薬シーズ開発の効率化に向けた次世代疾患モデルマウスの迅速作製技術開発
科学研究費補助金, JST A-Step 研究成果最適化支援事業
2009 - 2011 - アストラゼネカグラントリサーチ2009
2009 - 2009 - 多機能に関わるアドレノメデュリンーＲＡＭＰシステムの発生工学的病態解析と治療検討
科学研究費補助金, 基盤研究（Ｃ）
2008 - 2010 - RAMPによる代謝、血管制御メカニズムの解明と、代謝異常、血管合併症治療への展開
科学研究費補助金, 基盤研究（B）
2008 - 2010 - キメラマウスによる多機能ペプチド、アドレノメデュリンと関連因子の全組織包括的解析
科学研究費補助金, 基盤研究（Ｃ）
2006 - 2007 - 三井生命医学研究助成 アドレノメデユリンーRAMP2システムを標的とした､メタポリックシンドローム､動脈硬化症の新規治療法開発
2006 - 2006 - アストラゼネカグラントリサーチ2006
2006 - 2006 - マウス受精卵で転写を伴わないポリA鎖伸長を示す母性RNA群の多様性と生物学的意義
科学研究費補助金, 基盤研究（Ｃ）
2004 - 2005 - マウス初期胚における細胞質RNAの動的変化の重要性の解明
科学研究費補助金, 基盤研究（B）
2003 - 2005 - 受精後の初期発生プログラムに連動するポリA鎖伸長母性RNAの網羅的アプローチ
科学研究費補助金, 特定領域研究（C）
2001 - 2001 - マウス受精卵における時期特異的ポリ(A)鎖伸長母性RNA群の系統的解明
科学研究費補助金, 奨励研究（A)
2000 - 2001 - マウス初期胚母性RNAの時期特異的ポリ(A)鎖伸長の意義
科学研究費補助金, 特定領域研究（A)
2000 - 2000 - マウス着床前初期胚における遺伝子発現の総合的解析
科学研究費補助金, 国際学術
1996 - 1997 - Generation and characterization of new mouse models of human diseases
- Development of new technologies needed for generating gene-modified animal
- Understanding of Adrenomedullin/Ramps functions that are involved in cardiovascular diseases and inflammation
