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Tomotsune Daihachiro

Academic OrganizationAcademic Assembly School of Medicine and Health Sciences Institute of MedicineTEL+81-263-37-2590
Education and Research OrganizationInterdisciplinary Cluster for Cutting Edge Research, Institute for Biomedical Sciences , Department of Biotechnology and Biomedical EngineeringFAX+81-263-37-3093
PositionAssistant ProfessorMail
AddressDepartment of Histology and Embryology,Shinshu University of Medicine3-1-1 Asahi, Matsumoto, Nagano 390-8621, JapanWeb site


Research Field
Keywords:ES細胞 , iPS細胞 , 分化誘導
Current Subject
Differetiation of pluripotent stem cells

Academic Societies
Academic Societies
The Japanese Society for Regenerative Medicine
Academic Background
Graduate School
大阪大学大学院理学研究科 , 1994

理学博士 , 大阪大学
Research Career
Research Career
2007- , 信州大学医学部 助教
2006- , 信州大学医学部 助手
2002- , 千葉県がんセンター 研究員
2001- , 千葉大学医学部 研究員
2000- , 米国ベイラー医科大学 研究員
1996- , 大阪大学微生物病研究所 助手


Books, Articles, etc.
Induce Differentiation of Embryonic Stem Cells by Co-Culture System., Regenerative Medicine and Tissue Engineering , 117-139
Author:Yue F, Shirasawa S, Ichikawa H, Yoshie S, Mogi A, Masuda S, Nagai M, Yokohama T, Tomotsune D Sasaki K

A Strategy Using Pluripotent Stem Cell-Derived Hepatocytes for Stem Cell-Based Therapies, Advances in Regenerative Medicine , 145-164
Author:Tomotsune D., Sato F., Yoshie S., Shirasawa S., Yokoyama T., Kanoh Y., Ichikawa H., Mogi M., Yue F., Sasaki K.

Cell biological profiling of reprogrammed cancer stem cell-like colon cancer cells maintained in culture
2018(Oct. 08)
Author:Hirashima, K., Yue, F., Kobayashi, M., Uchida, Y., Nakamura, S., Tomotsune, D., Matsumoto, K., Takizawa, S., Yokoyama, T., Tani, R., Sasaki, K

Simulation of Embryonic Environments Produced by the Effect of Estradiol on Induced Pluripotent Stem Cells
2018(Jul. 01)
Author:Sakai, S., Tomotsune, D., Hirashima, K., Takizawa, S., Yokoyama, T., Yue, F., Tani, R., Matsumoto, K., Sasaki, K

Cardiac portion of the stomach: a deep region within the abdomen
Abdomen,First online 2017(Jul. 31)
Author:Hirashima K, Yue F, Tomotsune D, Sasaki K

Simple Isolation of Pancreatic Progenitor Cells from Human Induced Pluripotent Stem Cells Using the ALDEFLUOR.
Stem Cell Adv Res Ther.,:CRT-111 2017(Jan. 17)
Author:Nakamura, S., Takizawa-Shirasawa S., Yokoyama T., Tomotsune, D*., Sasaki,K.

Reprogramming of retinoblastoma cancer cells into cancer stem cells.
Biochem Biophys Res Commun.,:S0006-291X(16) 31928-3 2016(Nov. 14)
Author:Yue, F., Hirashima, K., Tomotsune D., Takizawa-Shirasawa S., Yokoyama T.,Sasaki,K

Earlobe-like peritoneal appendage near the angle of His: a useful landmark for demarcating the lateral margin of the gastric cardia.
Anatomical Science International.,:s12565-016-374-6 2016(Sep. 22)
Author:Hirashima, K., Yue, F., Tomotsune D., Sasaki K.

Enrichment of Pluripotent Stem Cell-Derived Hepatocyte-Like Cells by Ammonia Treatment.
PLoS One,11(9):e0162693 2016(Sep. 15)
Author:Tomotsune, D., Hirashima K., Fujii M., Yue F., Matsumoto K., Takizawa-Shirasawa S., Yokoyama T., Sasaki K.

New model for cardiomyocyte sheet transplantation using a virus-cell fusion technique
World J Stem Cells,7(5):883-893 2015
Author:Takahashi Y., Tomotsune D., Takizawa S., Yue F., Nagai M., Yokoyama T., Hirashima K., Sasaki K.

Effects of Fluid Dynamic Forces Created by Rotary Orbital Suspension Culture on Cardiomyogenic Differentiation of Human Embryonic Stem Cells
J. Med. Biol. Eng.,32(2):101-108 2014
Author:Mogi A, Takei S, Shimizu H, Miura H, Tomotsune D, Sasaki K

FGF7 and cell density are required for final differentiation of pancreatic amylase-positive cells from human ES cells.
Cell Tissue Res.,354:751-759 2013
Author:Takizawa-Shirasawa S, Yoshie S, Yue F, Mogi A, Yokoyama T, Tomotsune D, Sasaki K.

Unique kinetics of Oct3/4 microlocalization following dissociation of human embryonic stem cell colonies.
Ann Anat.,195:50-56 2012
Author:Ichikawa H, Kanoh Y, Shirasawa S, Yokoyama T, Yue F, Tomotsune D, Sasaki K.

Gene pathway analysis of the mechanism by which the Rho-associated kinase inhibitor Y-27632 inhibits apoptosis in isolated thawed human embryonic stem cells
Cryobiology,64(1):12-22 2012
Author:Ichikawa H, Nakata N, Abo Y, Shirasawa S, Yokoyama T, Yoshie S, Yue F, Tomotsune D, Sasaki K.

Establishment of novel detection system for embryonic stem cell-derived hepatocyte-like cells based on nongenetic manipulation with indocyanine green
Tissue Eng Part C Methods,18(1):12-20 2012
Author:Yoshie S, Ito J, Shirasawa S, Yokoyama T, Fujimura Y, Takeda K, Mizuguchi M, Matsumoto K, Tomotsune D, Sasaki K.

In vitro transdifferentiation of HepG2 cells to pancreatic-like cells by CCl2, D-galactosamine, and ZnCl2
Pancreas,40(8):1245-1252 2011
Author:Kanoh Y, Tomotsune D, Shirasawa S, Yoshie S, Ichikawa H, Yokoyama T, Mae S, Ito J, Mizuguchi M, Matsumoto K, Yue F, Sasaki K.

Pancreatic exocrine enzyme-producing cell differentiation via embryoid bodies from human embryonic stem cells
Biochem Biophys Res Commun,410(3):608-613 2011
Author:Shirasawa S, Yoshie S, Yue F, Ichikawa H, Yokoyama T, Nagai M, Tomotsune D, Hirayama M, Sasaki K

Freeze-thawing single human embryonic stem cells induce E-cadherin and actin filament network disruption viaG13 signaling
Cryo Letter,32(6):516-524 2011
Author:Ichikawa H, Yoshie S, Shirasawa S, Yokoyama T, Yue F, Tomotsune D, Sasaki K.

A novel potent tumour promoter aberrantly overexpressed in most human cancers
Scientific Reports,1(15):1-12 2011
Author:Takahashi A, Tokita H, Takahashi K, Takeoka T, Murayama K, Tomotsune D, Ohira M, Iwamatsu A, Ohara K, Yazaki K, Koda T, Nakagawara A, Tani K.

A novel stepwise differentiation of functional pancreatic exocrine cells from embryonic stem cells
Stem Cells Dev,20(6):1071-1078 2011
Author:Shirasawa S, Yoshie S, Yokoyama T, Tomotsune D, Yue F, Sasaki K

Bone marrow stromal cells as an inducer for cardiomyocyte differentiation from mouse embryonic stem cells
Ann Anat,192(5):314-321 2010
Author:Yue F, Johkuraa K, Tomotsune D, Shirasawa S, Yokoyama T, Nagai M, Sasaki K

Differentiation of primate ES cells into retinal cells induced by ES cell-derived pigmented cells
,394(4):877-883 2010
Author:Yue F, Johkura K, Shirasawa S, Yokoyama T, Inoue Y, Tomotsune D, Sasaki K

Combination of small molecules enhances differentiation of mouse embryonic stem cells into intermediate mesoderm through BMP7-positive cells
Biochem Biophys Res Commun,393(4):877-882 2010
Author:Mae S, Shirasawa S, Yoshie S, Sato F, Kanoh Y, Ichikawa H, Yokoyama T, Yue F, Tomotsune D, Sasaki K

Lanford medium induces high quality hepatic lineage cell differentiation directly from mouse embryonic stem cell-derivedmesendoderm
Biochem Biophys Res Commun,391(3):1477-1482 2010
Author:Yoshie S, Shirasawa S,Yokoyama T, Kanoh Y, Takei S, Mizuguchi M, Matsumoto K, Tomotsune D, Sasaki K

Hepatocyte differentiation from Human ES Cells using the simple embryoid body formation method and the staged-additional cocktail
ScientificWorld JOURNAL,9:884-890 2009
Author:Sasaki K, Ichikawa H, Takei S, No H-S, Tomotsune D, Kano Y, Yokoyama T, Sirasawa S, Mogi A, Yoshie S, Sakaki S, Yamada S, Matsumoto K, Mizuguchi M, Yue F, Tanaka Y

Bone morphogenetic protein-4 promotes induction of cardiomyocytes from human embryonic stem cells in serum-based embryoid body development
Am J Physiol Heart Circ Physiol,296(6):H1793-803 2009
Author:Takei S, Ichikawa H, Johkura K, Mogi A, No H, Yoshie S, Tomotsune D, Sasaki K

The method of mouse embryoid body establishment affects structure and developmental gene expression
Tissue Cell,41(1):79-84 2009
Author:Mogi A, Ichikawa H, Matsumoto C, Hieda T, Tomotsune D, Sakaki S, Yamada S, Sasaki K

Increased expression of proapoptotic BMCC1, a novel gene with the BNIP2 and Cdc42GAP homology (BCH) domain, is associated with favorable prognosis in human neuroblastomas
ONCOGENE,25(13):1931-1942 2006
Author:Machida, T; Fujita, T; Ooo, ML; Ohira, M; Isogai, E; Mihara, M; Hirato, J; Tomotsune, D; Hirata, T; Fujimori, M; Adachi, W; Nakagawara, A

第21回 再生医療学会 2022(Mar.)
Presenter:藤本 俊介、正田 雅輝、鬼柳 由美子、冨田 優太、岳 鳳鳴、友常 大八郎、滝澤 佐季子

第20回再生医療学会総会 2021(Mar.)

第20回再生医療学会総会 2021(Mar.)
Presenter:滝澤佐季子、藤本俊介、岳 鳳鳴、鬼柳由美子、冨田優太、友常 大八郎

第20回再生医療学会総会 2021(Mar.)
Presenter:藤本俊介、正田 雅輝、鬼鬼柳由美子、冨田優太、岳 鳳鳴、友常 大八郎、滝澤佐季子

第43回日本分子生物学会年会 2020(Dec.)
Presenter:Shunsuke Fujimoto, Mieko Takasaka, Yumiko Kiyanagi, Yuta Tomita, Fengming Yue, Daihachiro Tomotsune, Katsunori Sasaki, Naoto Saito, Sakiko Takizawa-Shirasawa

ISSCR 2020(Jun.)
Presenter:S., Fujimoto, M., Takasaka, Y., Kiyanagi, Y., T., F., Yue, D., Tomotsune, K., Sasaki, N., Saito, S., Takizawa-Shirasawa

Purification of ALDH positive pancreatic cancer stem cells using ALDEFLUOR
第18回 日本再生医療学会 2020(Mar.)
Presenter:岳鳳鳴、佐々木志織、城倉 浩平、友常 大八郎

Research Grants
Grants‐in‐aid for Scientific Research(Research Representative)
2019 - 2021 , 多能性幹細胞の分化状態変化におけるポリコーム遺伝子の役割
2015 - 2017 , ヒト多能性幹細胞におけるMBTファミリーの役割 , 基盤研究(C)

Grants‐in‐aid for Scientific Research(Researcher)
28 - 29 , 骨粗鬆症患者における個人差研究のためのリプログラミング因子の探索 , 文部科学省挑戦的萌芽
27 - 30 , 多能性幹細胞を用いた新規副次肝作成技術開発―腸管の肝臓化― , 文部科学省基盤研究A