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山本博規  ヤマモト ヒロキ

教員組織学術研究院(繊維学系)電話番号
教育組織繊維学部 応用生物科学科FAX番号
職名准教授メールアドレス
住所長野県上田市常田3-15-1ホームページURL

更新日:2019/08/07

プロフィール

兼担研究科・学部
大学院総合工学系研究科
研究分野
資源微生物学
環境微生物学
ゲノム微生物学
現在の研究課題
微生物資源の有効利用
キーワード:グラム陽性細菌 環境微生物 バイオフィルム , 2007-
枯草菌の細胞表層修飾と細胞形態維持機構の研究
キーワード:細胞形態 細胞壁修飾機構 , 2006-
細胞表層タンパク質の局在化機構の解明
キーワード:局在機構 細胞表層タンパク質 , 2003-
所属学会
所属学会
日本農芸化学会
日本生物工学会
日本ゲノム微生物学会
学歴
出身大学院
1991 , 信州大学 , 繊維学研究科

出身学校・専攻等(大学院を除く)
1989 , 信州大学 , 繊維学部

取得学位
工学博士 , 信州大学
農学修士 , 信州大学
農学士 , 信州大学
研究職歴等
研究職歴
2008- , 信州大学繊維学部准教授
2007-2008 , 信州大学繊維学部附属農場 准教授
2005-2007 , 信州大学大学院総合工学系研究科 助手
1994-2005 , 信州大学繊維学部 助手
1991-1994 , 鐘紡株式会社生化学研究所 研究員

留学歴
2003-2004 , パスツール研究所

研究活動業績

研究業績(著書・
発表論文等)
著書
Cell wall structure of E. coli and B. subtilis
Kerala: Research Signpost , In: Y. Sadaie and K. Matsumoto, editors, The Frontiers of Molecular Microbiology Revisited , :115-148 2012
Author:Sekiguchi, J., and H. Yamamoto


Murein Peptidase LytF
Oxford: Academic Press , In: Neil D. Rawlings and Guy S. Salvesen, editors, Handbook of Proteolytic Enzymes, 3rd Edition. , :2479-2482 2012
Author:Sekiguchi, J., and H. Yamamoto


バイオマスとその利用
オーム社 , 応用生物科学入門 , :第6章 135-157頁 2010
Author:白井孝治、山本博規


Northern Blot Analysis with non-RI Detection Systems; Functional Analysis of Bacterial Genes
A Practical Manual, Chap. 5B, John Wiley&Sons , :41-44 2001
Author:W. Schumann/S. D. Ehrlich /N. Ogasawara eds./ Yamamoto, H./K. Asai /K. Kobayashi


論文
The transmembrane segment of TagH is required for wall teichoic acid transport under heat stress in Bacillus subtilis
Microbiology,164:935-945 2018
Author:Yamada, T., Miyashita, M., Kasahara, J., Tanaka, T., Hashimoto, M., Yamamoto, H.


Digestion of peptidoglycan near the cross-link is necessary for the growth of Bacillus subtilis
Microbiology,164:299~307 2018
Author:Hashimoto, M., Matsushima, H., Suparthana I. P., Ogasawara, H., Yamamoto, H., Teng, C., Sekiguchi, J.


Application of freeze-dried powders of genetically engineered microbial strains as adsorbents for rare earth metal ions
ACS Appl. Mater. Interfaces,8(40):26524-26531 2016
Author:Moriwaki, H., Masuda, R., Yamazaki, Y., Horiuchi, K., Miyashita, M., Kasahara, J., Tanaka, T., and Yamamoto, H.


Teichoic acid polymers affect expression and localization of DL-endopeptidase LytE required for lateral cell wall hydrolysis in Bacillus subtilis.
J. Bacteriol.,198(11):1585-1594 2016
Author:Kasahara, J., Y. Kiriyama, M. Miyashita, T. Kondo, T. Yamada, K. Yazawa, R. Yoshikawa and H. Yamamoto.


分泌シグナルに基づく細胞表層タンパク質のターゲティング機構の解明
IFO Research Communications,28:132-133 2014
Author:山本博規


Localization and expression of the Bacillus subtilis DL-endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes.
Microbiology,160:2639-2649 2014
Author:Kiriyama, Y., K. Yazawa, T. Tanaka, R. Yoshikawa, H. Yamane, M. Hashimoto, J. Sekiguchi, and H. Yamamoto.


A cell wall protein (YqgA) is genetically related to the cell wall-degrading DL-endopeptidases in Bacillus subtilis.
Biosci. Biotech. Biochem.,78(8):1428-1434 2014
Author:Hashimoto, M., K. Fujikura, Y. Miyake, Y. Higashitsuji, Y. Kiriyama, T. Tanaka, H. Yamamoto and J. Sekiguchi.


Interactions of microorganisms with rare earth ions and their utilization for separation and environmental technology
Appl. Microbiol. Biotech.,97(1):1-8 2013
Author:Moriwaki, H., and H. Yamamoto


Adsorption of rare earth ions onto the cell walls of wild type and lipoteichoic acid-defective strains of Bacillus subtilis
Appl. Microbiol. Biotech.,97(8):3721-3728 2013
Author:Moriwaki, H., R. Koide, R. Yoshikawa, Y. Warabino and H. Yamamoto


バクテリアにおける細胞骨格蛋白質の機能解明
倉田奨励金研究報告,41:47-49 2011
Author:山本博規


バクテリアにおける細胞骨格蛋白質の機能解明
倉田奨励金研究報告,40:99-101 2010
Author:山本博規


細菌細胞壁の合成・修飾・分解機構の解明
財団法人長瀬科学技術振興財団研究報告集,21:89-96 2010
Author:山本博規


枯草菌における細胞の分離
繊維学会誌,65:282-286 2009
Author:橋本昌征,山本博規,関口順一


The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis.
MOLECULAR MICROBIOLOGY,70(2):297-310 2008
Author:Yamamoto, H., Y. Miyake, M. Hisaoka, S. Kurosawa and Junichi Sekiguchi.


Post-translational control of vegetative cell separation enzymes through a direct interaction with specific inhibitor IseA in Bacillus subtilis.
MOLECULAR MICROBIOLOGY,70(1):168-182 2008
Author:Yamamoto, H., M. Hashimoto, Y. Higashitsuji, H. Harada, N. Hariyama, L. Takahashi, T. Iwashita, S. Ooiwa and J. Sekiguchi.


Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities
JOURNAL OF BIOLOGICAL CHEMISTRY,283(17):11117-11125 2008
Author:Fukushima, T., Kitajima, T., H. Yamaguchi, Q. Ouyang, K. Furuhata, H. Yamamoto, T. Shida, and J. Sekiguchi


Characterization of new L,D-endopeptidase gene product CwlK (previous YcdD) that hydrolyzes peptidoglycan in Bacillus subtilis
MOLECULAR GENETICS AND GENOMICS,278(4):371-383 2007
Author:Fukushima, T; Yao, Y; Kitajima, T; Yamamoto, H; Sekiguchi, J


A new D,L-endopeptidase gene product, YojL (renamed CwlS), plays a role in cell separation with LytE and LytF in Bacillus subtilis
J. Bacteriol.,188(15):5541-5550 2006
Author:Fukushima, T., A. Afkham, S. Kurosawa, T. Tanabe, H. Yamamoto, and J. Sekiguchi.


Functional analysis of the YvrGHb two component system of Bacillus subtilis: Identification of the regulated genes by DNA microarray and Northern blot analyses
Biosci. Biotechnol. Biochem.,59(11):2155-2169 2005
Author:Serizawa, M., K. Kodama, H. Yamamoto, K. Kobayashi, N. Ogasawara, and J. Sekiguchi.


Characterization of a polysaccharide deacetylase gene homologue (pdaB) on sporulation of Bacillus subtilis
J. Biochemistry (Tokyo),136(3):283-291 2004
Author:Fukushima, T., T. Tanabe, H. Yamamoto, S. Hosoya, T. Sato, H. Yoshikawa and J. Sekiguchi


Characterization of a new Bacillus subtilis peptidoglycan hydrolase gene, yvcE (named cwlO), and the enzymatic properties of its encoded protein
J. Biosci. Bioeng.,98(3):174-181 2004
Author:Yamaguchi, H., K. Furuhata, T. Fukushima, H. Yamamoto, and J. Sekiguchi


Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses
Gene,329:125-136 2004
Author:Serizawa, M., H. Yamamoto, H. Yamaguchi, Y. Fujita, K. Kobayashi, N. Ogasawara, and J. Sekiguchi


Localization of vegetative cell wall hydrolases, LytC, LytE and LytF, on the Bacillus subtilis cell surface, and the stability of these enzymes to cell wall-bound or extracellular proteases
J. Bacteriol.,185(22):6666-6677 2003
Author:Yamamoto, H., S. Kurosawa, and J. Sekiguchi


Transcriptional, functional and cytochemical analyses of the veg gene in Bacillus subtilis
J. Biochemistry (Tokyo),133(4):475-483 2003
Author:Fukushima, T., S. Ishikawa, H. Yamamoto, N. Ogasawara, and J. Sekiguchi


Essential Bacillus subtilis genes
Proc. Natl. Acad. Sci. USA,100(8):4678-4683 2003
Author:Kobayashi, K., T. Fukushima, S. Ishikawa, J. Sekiguchi, H. Yamamoto, N. Ogasawara (他93名)


枯草菌はどのように環境変化に適応するか ー細胞表層の蛋白質の転写を制御する二成分制御系が明らかにー
化学と生物,40(12):778-780 2002
Author:山本博規;関口順一


A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic _-lactam residues in the spore cortex of Bacillus subtilis
J. Bacteriol.,184(21):6007-6015 2002
Author:Fukushima, T., H. Yamamoto, A. Atrih, S. J. Foster, and J. Sekiguchi


Stabilization of cell wall proteins in Bacillus subtilis: a proteomic approach
Proteomics,2(5):591-602 2002
Author:Antelmann, H., H. Yamamoto, J. Sekiguchi, and M. Hecker


Simultaneous display of bacterial and fungal lipases on the cell surface of Bacillus subtilis
J. Biosci. Bioeng.,93(1):15-19 2002
Author:Kobayashi, G., K. Fujii, M. Serizawa, H. Yamamoto, and J. Sekiguchi


Production of long-chain levan by a sacC insertional mutant from Bacillus subtilis 327UH
Biosci. Biotechnol. Biochem.,66(7):1555-1558 2002
Author:Shida, T., K. Mukaijo, S. Ishikawa, H. Yamamoto, and J. Sekiguchi


Regulation of the glv operon in Bacillus subtilis: YfiA (GlvR) is a positive regulator of the operon that is repressed through CcpA and cre
J. Bacteriol.,183(17):5110-5121 2001
Author:H. Yamamoto/ M. Serizawa/ J. Thompson / J. Sekiguchi


Accumulation of a recombinant Aspergillus oryzae lipase artificially localized on the Bacillus subtilis cell surface
J. Biosci. Bioeng.,90:422-425 2000
Author:G. Kobayashi/J. Toida/T. Akamatsu/ H. Yamamoto/T. Shida / J. Sekiguchi


The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
MOLECULAR MICROBIOLOGY,37(4):898-912 2000
Author:Yamamoto, H; Murata, M; Sekiguchi, J


Accumulation of an artificial cell wall-binding lipase by Bacillus subtilis wprA and/or sigD mutants
FEMS Microbiol. Letters,188(2):165-169 2000
Author:Kobayashi, G; Toida, J; Akamatsu, T; Yamamoto, H; Shida, T; Sekiguchi, J


枯草菌のゲノムサイエンス
生物工学会誌,77(9):399-402 1999
Author:関口順一;山本博規


枯草菌ゲノムと遺伝子の特徴
蛋白質・核酸・酵素,44(10):1449-1459 1999
Author:小笠原直毅;定家義人;藤田昌也;吉田健一;藤田泰太郎;吉川博文;三輪泰彦;山本博規;関口順一;熊野みゆき;山根國男;村田麻喜子;大木玲子


Transcription of genes near the sspE locus of the Bacillus subtilis genome
Microbiology,145:2171-2180 1999
Author:Yamamoto, H., M. Mori, and J. Sekiguchi


Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface
FEMS Microbiol Letters,176(2):373-378 1999
Author:Tsuchiya, A; Kobayashi, G; Yamamoto, H; Sekiguchi, J


Characterization of a new sigma K-dependent cell wall hydrolase gene that plays a role in Bacillus subtilis mother cell lysis
JOURNAL OF BACTERIOLOGY,181(20):6230-6237 1999
Author:Nugroho, FA; Yamamoto, H; Kobayashi, Y; Sekiguchi, J


Crystallization and preliminary X-ray analysis of the 6-phospho-alpha-glucosidase from Bacillus subtilis
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,55:1212-1214 1999
Author:Varrot, A; Yamamoto, H; Sekiguchi, J; Thompson, J; Davies, GJ


The gene glvA of Bacillus subtilis 168encodes a metal-requiring, NAD (H)-dependent 6-phospho-alpha-glucosidase - Assignment to family 4 of the glycosylhydrolase superfamily
JOURNAL OF BIOLOGICAL CHEMISTRY,273(42):27347-27356 1998
Author:Thompson, J; Pikis, A; Ruvinov, SB; Henrissat, B; Yamamoto, H; Sekiguchi, J


The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
NATURE,390(6657):249-256 1997
Author:Kunst, F; Ogasawara, N; Moszer, I; Albertini, AM; Alloni, G; Azevedo, V; Bertero, MG; Bessieres, P; Bolotin, A; Borchert, S; Borriss, R; Boursier, L; Brans, A; Braun, M; Brignell, SC; Bron, S; Brouillet, S; Bruschi, CV; Caldwell, B; Capuano, V; Carter, NM; Choi, SK; Codani, JJ; Connerton, IF; Cummings, NJ; Daniel, RA; Denizot, F; Devine, KM; Dusterhoft, A; Ehrlich, SD; Emmerson, PT; Entian, KD; Errington, J; Fabret, C; Ferrari, E; Foulger, D; Fritz, C; Fujita, M; Fujita, Y; Fuma, S; Galizzi, A; Galleron, N; Ghim, SY; Glaser, P; Goffeau, A; Golightly, EJ; Grandi, G; Guiseppi, G; Guy, BJ; Haga, K; Haiech, J; Harwood, CR; Henaut, A; Hilbert, H; Holsappel, S; Hosono, S; Hullo, MF; Itaya, M; Jones, L; Joris, B; Karamata, D; Kasahara, Y; KlaerrBlanchard, M; Klein, C; Kobayashi, Y; Koetter, P; Koningstein, G; Krogh, S; Kumano, M; Kurita, K; Lapidus, A; Lardinois, S; Lauber, J; Lazarevic, V; Lee, SM; Levine, A; Liu, H; Masuda, S; Mauel, C; Medigue, C; Medina, N; Mellado, RP; Mizuno, M; Moestl, D; Nakai, S; Noback, M; Noone, D; OReilly, M; Ogawa, K; Ogiwara, A; Oudega, B; Park, SH; Parro, V; Pohl, TM; Portetelle, D; Porwollik, S; Prescott, AM; Presecan, E; Pujic, P; Purnelle, B; Rapoport, G; Rey, M; Reynolds, S; Rieger, M; Rivolta, C; Rocha, E; Roche, B; Rose, M; Sadaie, Y; Sato, T; Scanlan, E; Schleich, S; Schroeter, R; Scoffone, F; Sekiguchi, J; Sekowska, A; Seror, SJ; Serror,P; Shin, BS; Soldo, B; Sorokin, A; Tacconi, E; Takagi, T; Takahashi, H; Takemaru, K; Takeuchi, M; Tamakoshi, A; Tanaka, T; Terpstra, P; Tognoni, A; Tosato, V; Uchiyama, S; Vandenbol, M; Vannier, F;Vassarotti, A; Viari, A; Wambutt, R; Wedler, E; Wedler, H; Weitzenegger, T; Winters, P; Wipat, A; Yamamoto, H; Yamane, K; Yasumoto, K; Yata, K; Yoshida, K; Yoshikawa, HF; Zumstein, E; Yoshikawa, H; Danchin, A


Cloning and sequencing of a 35.7 kb in the 70 degree - 73 degree region of the Bacillus subtilis genome reveal genes for a new two-component system, three spore germination protein, an iron uptake system and a stress response protein
Gene,194(2):191-199 1997
Author:Hiroki Yamamoto;Shigeki Uchiyama;Fajar Aji Nugroho;Junichi Sekiguchi


A 23.4 kb segment at the 69 degree to 70 degree region of the Bacillus subtilis genome
Microbiology,143(4):1317-1320 1997
Author:Yamamoto, H; Uchiyama, S; Nugroho, FA; Sekiguchi, J


The Bacillus subtilis chromosome region near 78 degrees contains the genes encoding a new two-component system, three ABC transporters and a lipase
GENE,181(1-2):147-151 1996
Author:Yamamoto, H; Uchiyama, S; Sekiguchi, J


Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degree - 81 degree region of the Bacillus subtilis genome containing the sspE locus
DNA Res.,3:257-262 1996
Author:Hiroki Yamamoto;Shigeki Uchiyama;Junichi Sekiguchi


Cloning and sequencing of a 40.6 kb segment in the 73 degree - 76 degree region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization
Microbiology,142(11):3057-3065 1996
Author:Hiroki Yamamoto;Shigeki Uchiyama;Junichi Sekiguchi


Determination of a 12 kb nucleotide sequence around the 76 degrees region of the Bacillus subtilis chromosome
MICROBIOLOGY-UK,142:1417-1421 1996
Author:Yamamoto, H; Uchiyama, S; Fajar, AN; Ogasawara, N; Sekiguchi,J


Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis
J. Bacteriol.,177(19):5582-5589 1995
Author:Junichi Sekiguchi;Kazumi Akeo;Hiroki Yamamoto;Fuat K. Khasanov;Juan C. Alonso;Akio Kuroda


Nucleotide sequence and characterization of the large mitochondrial rRNA gene of Penicillium urticae, and its comparison with those of other filamentous fungi
J. Biochem.,117(4):888-896 1995
Author:Hiroki Yamamoto;Atsushi Naruse;TakeshiOhsaki;Junichi Sekiguchi


Nucleotide sequence of the large mitochondrial rRNA gene of Penicillium chrysogenum
Biochim. Biophys. Acta,1172:353-356 1993
Author:Naruse, A., H. Yamamoto, and J. Sekiguchi.


Autonomously replicating fragments of mitochondrial DNA from Penicillium urticae in Saccharomyces cerevisiae
J. Ferment. Bioeng.,71:275-277 1991
Author:Yamamoto, H., Y. Yoshida, Y. Kitamura, and J. Sekiguchi.


Transformation of Penicillium urticae with plasmids containing the hygromycin B resistance gene
Agric. Biol. Chem.,55:3053-3057 1991
Author:Kiuchi, N., A. Naruse, H. Yamamoto, and J. Sekiguchi.


CHARACTERIZATION AND COMPARISON OF MITOCHONDRIAL DNAS AND RIBOSOMAL-RNAS FROM PENICILLIUM-URTICAE AND PENICILLIUM-CHRYSOGENUM
JOURNAL OF GENERAL MICROBIOLOGY,136:535-543 1990
Author:Sekiguchi, J., T. Ohsaki, H. Yamamoto, K. Koichi, and T. Shida.


学会発表
Proteinaceous inhibitor affecting activity of cell separation enzymes in Bacillus subtilis
4th Conference on Functional Genomics of Gram-Positive Microorganisms , :T42 2007(Jun.)
Author:J.Sekiguchi, T.Iwashita, H.Yamamoto

共同研究等希望テーマ
微生物資源の有効利用
環境微生物ゲノム解析
研究費
受託研究
2011 - 2011 , 植物資源の有効利用に関する研究
2008 - 2011 , 細菌の細胞表層ポリマーに関する研究
2008 - 2010 , 乳酸菌発酵液が植物の生育に及ぼす影響調査
2007 - 2007 , 生ゴミ堆肥化過程における微生物の変動調査

科学研究費補助金(研究代表者)
2017 - 2019 , 細菌細胞壁のテイコ酸修飾機構の解明および高温環境適応におけるテイコ酸の重要性 , 基盤研究(C)
2011 - 2013 , 細菌細胞表層タンパク質のターゲティング機構の解明 , 基盤研究(C)
2007 - 2009 , 細菌細胞壁のテイコ酸修飾における細胞骨格蛋白質の機能解明 , 基盤研究(C)
2002 - 2004 , 枯草菌細胞表層蛋白質の安定性及び局在性における分泌プロテアーゼの影響 , 若手研究(B)

科学研究費補助金(研究分担者)
2017 - 2019 , 枯草菌遺伝子操作株を滅菌・凍結乾燥させた粉体によるレアメタルの抽出法の開発 , 基盤研究(C)
2010 - 2012 , 細菌細胞表層制御による細胞増殖,形態,機能維持メカニズムの解明 , 基盤研究(A)
2007 - 2009 , 細菌細胞の形を支配する新規表層蛋白質の解析 , 基盤研究(B)
2004 - 2006 , 細菌細胞の分離メカニズムとタイコイン酸の機能の研究 , 基盤研究(B)
2001 - 2003 , 細菌細胞表層の合成・修飾機構の研究 , 基盤研究(B)
2000 - 2002 , 細胞表層・細胞膜を介した環境との相互ネットワークの解析 , 特定領域研究(C)

奨学寄附金
2012 , 細胞表層タンパク質のターゲティング機構の解明
2011 , バクテリアにおける細胞表層修飾機構の解明
2009 , バクテリアにおける細胞骨格蛋白質の機能解明
2009 , 細菌細胞壁の合成・修飾・分解機構の解明
2007 , 枯草菌の細胞分離酵素の局在性と細胞壁修飾機構の解明

教育活動実績

授業等
2016 , 前期 , 環境微生物学
2016 , 前期 , バイオマス資源論
2016 , 前期 , 生物資源・環境科学実験I
2016 , 前期 , 新入生ゼミナール
2016 , 前期 , 応用生物学への招待
2016 , 後期 , 微生物学
2016 , 後期 , 生物科学基礎実験
2016 , 後期 , 生物資源・環境科学実験II