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田口悟朗  タグチ ゴロウ

教員組織学術研究院(繊維学系)電話番号0268-21-5342
教育組織繊維学部 応用生物科学科FAX番号
職名准教授メールアドレスgtagtag@shinshu-u.ac.jp
住所〒386-8567 長野県上田市常田3-15-1ホームページURLhttp://fiber.shinshu-u.ac.jp/taguchi/index.html

更新日:2019/05/29

プロフィール

兼担研究科・学部
大学院総合理工学研究科
大学院総合工学系研究科
研究分野
応用生物化学
植物生化学
植物分子生物学
現在の研究課題
植物の低分子化合物修飾酵素反応の解析
キーワード:配糖化酵素 , アシル化酵素
植物における異物解毒機構の研究
キーワード:異物代謝 , 解毒 , 修飾酵素 , 蓄積
植物の香気成分生合成の解析
キーワード:香気成分 , 酵素反応
キノコの遺伝子操作系の開発
キーワード:きのこ
ヒカリゴケの人工培養に関する研究
キーワード:ヒカリゴケ
所属学会
所属学会
日本農芸化学会
日本植物生理学会
日本生物工学会
日本植物細胞分子生物学会

所属学会役職担当
2015-2019 , 日本生物工学会 , 代議員
2012-2016 , 日本植物細胞分子生物学会 , 評議員
2005-2018 , 日本生物工学会中部支部 , 企画幹事
学歴
出身大学院
1993 , 京都大学 , 薬学研究科

出身学校・専攻等(大学院を除く)
1992 , 京都大学 , 薬学部

免許・資格等
1992 , 薬剤師
受賞学術賞
2006 , 日本植物細胞分子生物学会奨励賞
研究職歴等
研究職歴
2008- , 信州大学繊維学部 准教授
2005-2008 , 信州大学繊維学部 講師
2003-2004 , 信州大学ヒト環境科学研究支援センター 助手
1996-2003 , 信州大学遺伝子実験施設 助手
1993-1996 , 信州大学繊維学部 助手

留学歴
2005-2006 , ミシガン大学 客員研究員

研究活動業績

研究業績(著書・
発表論文等)
著書
Flavonoid Biosynthesis in Buckwheat. (Chapter 30)
Academic Press (Elsevier) , Molecular Breeding and Nutritional Aspects of Buckwheat (Zhou et al. Eds) , :377-386 2016
Author:Goro Taguchi


植物の異物修飾機構を応用した配糖体生産法.
シーエムシー出版 , 濱田博喜監修『機能性配糖体の合成と応用−糖転移酵素を中心に−』 , :5-15 2013
Author:田口悟朗


生物工学実験書改訂版
培風館 , :164-172 2002(Apr.)
Author:日本生物工学会編 (岡崎光雄,田口悟朗)


Effect of cork tissues on the production of secondary metabolites in various plantcell cultures.
Recent Res. Devel. Biotech.&Bioeng. , 2:273-281 1999
Author:H. Yamamoto, K. Yazaki, H. Hayashi, G. Taguchi, A. Yato, K. Inoue


論文
Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)
Biosci. Biotechnol. Biochem.,82(10):1790-1802 2018
Author:Koja, E., Ohata, S., Maruyama, Y., Suzuki, H., Shimosaka, M., Taguchi, G.


C-Glycosyltransferases catalyzing the formation of di- C-glucosyl flavonoids in citrus plants.
Plant J,91(2):187-198 2017
Author:Takamitsu Ito, Shunsuke Fujimoto, Fumiaki Suito, Makoto Shimosaka, Goro Taguchi


Taste characterization of the fruit body derived from a novel strain belonging to the genus Pleurotus
Mushroom Sci Biotechnol,24(3):129-135 2016
Author:Yoshihito Azumi, Kahori Doi, Kana Kogiso, Goro Taguchi, Makoto Shimosaka, Kenji Ouchi, Satoshi Inatomi


pFungiway: a series of plasmid vectors used for gene manipulation in fungi.
Annals of Microbiology,66(2):825–832 2016
Author:Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Toru Okuhara, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka


Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C- glucosyltransferases
Plant Biotechnol.,31(5):519-524 2014
Author:Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.


Purification, molecular cloning and functional characterization of flavonoid-C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
Plant J,80(3):437-448 2014
Author:Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.


Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
J. Biosci. Bioeng.,115(4):360-365 2013
Author:Sekiya, S., Yamada, M., Shibata, K., Okuhara, T., Yoshida, M., Inatomi, S., Taguchi, G., Shimosaka, M.


Structural basis for modification of flavonol and naphthol glucoconjugates by Nicotiana tabacum malonyltransferase (NtMaT1).
Planta,236:781-793 2012
Author:Manjasetty, B.A., Yu, X.-H., Panjikar, S., Taguchi, G., Chance, M.R., Liu, C.-J.


Contribution of CoA ligases to benzenoid biosynthesis in petunia flowers.
Plant Cell,24:2015-2030 2012
Author:Klempien, A., Kaminaga, Y., Qualley, A., Nagegowda, D. A., Widhalm, J. R., Orlova, I., Kumar-Shasany, A., Taguchi, G., Kish, C.M., Cooper, B.R., D’Auria, J.C., Rhodes, D., Pichersky, E., Dudareva, N.


Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium, Chitiniphilus shinanonensis
Biosci. Biotech. Biochem.,76:517-522 2012
Author:Huang L., Shizume, A., Nogawa, M., Taguchi, G., Shimosaka, M.


Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold, Aspergillus oryzae
Biosci. Biotech. Biochem.,76:193-195 2012
Author:Akihiro Sugita, A., Sugii, A., Sato, K., Zhang, X.-Y., Dai, A.-L., Taguchi, G., Shimosaka, M


Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase.
J. Biosci. Bioeng.,113:293-299 2011(Feb. 12)
Author:Huang L., Garbulewska, E., Sato, K., Kato , Y., Shizume, A., Nogawa, M., Taguchi, G., Shimosaka, M.


Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of cultivated mushroomFlammulina velutipes
Biosci. Biotech. Biochem.,74(11):2327-2329 2010(Nov.)
Author:Okamoto, T., Yamada, M., Sekiya,S., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M.


Malonylation is a key reaction in the metabolism of xenobiotic phenolic glucosides in Arabidopsis and tobacco
Plant J.,63(6):1031-1041 2010(Sep.)
Author:Taguchi, G., Ubukata,T., Nozue, H., Kobayashi, Y., Takahi, M., Yamamoto, H., Hayashida, N.


Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
J. Biosci. Bioeng.,109(5):472-478 2010(May)
Author:Sato K., Azama Y., Nogawa M., Taguchi G., Shimosaka M.


Suppression of Arogenate Dehydratase1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals.
Plant Cell,22(3):832-849 2010(Mar.)
Author:MaedaH, Shasany AK, Schnepp J, Orlova I, Taguchi G, Cooper BR, Rhodes D, Pichersky E, Dudareva N.


Construction and analysis of a bacterial community exhibiting a strong chitinolytic activity
Biosci. Biotech. Biochem.,74(3):636-640 2010(Mar.)
Author:Sato, K., Kato, Y., Fukamachi, A., Nogawa, M., Taguchi, G., Shimosaka M.


Agrobacterium tumefaciens-mediated transformation of antifungal lipopeptide producing fungus Coleophoma empetri.
Current Genetics,55(6):623-630 2009(Dec.)
Author:Yamada, M., Yawata, K., Orino, Y., Ueda, S., Isogai, Y., Taguchi, G. Shimosaka, M, Hashimoto,S.


Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
J. Gen. Appl. Microbiol.,55(2):147-153 2009(May)
Author:Sato, K., Kato, Y.,Taguchi, G., Nogawa, M., Yokota, A., Shimosaka, M.
Keywords:Betaproteobacteria,chitin,Chitiniphilus shinanonensis gen. nov., sp. Nov,chitinolytic bacterium,Neisseriaceae,16S rRNA gene sequence


Construction of a Practical SCAR Marker Linked toClubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes
J. Japan. Soc. Hort. Sci.,77(2):150-154 2008(Apr.)
Author:Hayashida, N., Takabatake, Y., Nakazawa, N., Aruga, D., Nakanishi, H., Taguchi, G., Sakamoto, K., Matsumoto, E.
Keywords:clubroot resistance,HC352,paralog,sequence characterized amplified region (SCAR),CRa


Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
FEMS Microbiol Lett.,289:130-137 2008
Author:Yamada, M., Kurano, M., Inatomi, S., Taguchi, G., Okazaki, M., Shimosaka,M.


Characterization of a gene conferring red fluorescence isolated from an environmental DNA library constructed from soil bacteria.
Biosci. Biotech. Biochem.,72:1908-1914 2008
Author:Lan, X.-Q., Sato, K. Taguchi, G., Zhou,Z.-Y., Shimoaka, M.


Mapping of Isolate-Specific QTLs for Clubroot Resistance in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).
Theor. Appl. Genet.,117:759-767 2008
Author:Sakamoto, K., Saito, A., Taguchi, G., Hayashida, N., Matsumoto, E.


Convergent evolution in the BAHD family of acyl transferases: identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana
Plant J.,50(4):678-695 2007
Author:Luo, J; Nishiyama, Y; Fuell, C; Taguchi, G; Elliott, K; Hill, L; Tanaka, Y; Kitayama, M; Yamazaki, M; Bailey, P; Parr, A; Michael, AJ; Saito, K;Martin, C


Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display
FEMS Microbiol. Lett.,254(1):165-172 2006
Author:Masato Yamada;Sou Sakuraba;Kou Shibata;Goro Taguchi;Satoshi Inatomi;Mitsuo Okazaki;Makoto Shimosaka


Molecular cloning, characterization, and downregulation of an acyltransferase that catalyzes the malonylation of flavonoid and naphthol glucosides in tobacco cells.
Plant J.,42:481-491 2005(May)
Author:Goro Taguchi, Yoshihiro Shitchi, Sakiko Shirasawa, Hirobumi Yamamoto, Nobuaki Hayashida


Characterization of the Arabidopsis thaliana mutant pcb2 which accumulates divinyl chlorophylls.
Plant Cell Physiol.,46(3):467-473 2005(Mar.)
Author:Hiromitsu Nakanishi, Hatsumi Nozue, Kenji Suzuki, Yasuko Kaneko, Goro Taguchi, Nobuaki Hayashida


Metabolism of administered (2RS)-naringenin in flavonoidproducing cultured cells of Sophora flavescens
Plant Biotech.,21(5):355-359 2004
Author:Hirobumi Yamamoto, Hiroki Kuribayashi, Yasuharu Seshima, Ping Zhao, Isao Kouno, Goro Taguchi, Koichiro Shimomura
Keywords:Naringenin, (2R)-naringenin 4′,7-di-O-β-D-glucoside, Sophora flavescens, sophoraflavanone G


Collection and analysis of Arabidopsis color mutants.
Endocytobiosis Cell Res,15(1):328-338 2004
Author:Hiromitsu Nakanishi, Kenji Suzuki, Takashi Jouke, Ritsuko Kodaira, Goro Taguchi, Mitsuo Okazaki, Nobuaki Hayashida


Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.
Arch. Biochem. Biophys.,420(1):95-102 2003(Nov.)
Author:Goro Taguchi, Takahisa Ubukata, Nobuaki Hayashida, Hirobumi Yamamoto, Mitsuo Okazaki


Exogenously added naphthols induce three glucosyltransferases, and are accumulated as glucosides in tobacco cells.
Plant Science,164:231-240 2003
Author:Goro Taguchi, Minako Nakamura, Nobuaki Hayashida, Mitsuo Okazaki


Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis.
Glycobiology,13:139-145 2003
Author:E.K. Lim, S. Baldauf, Y. Li, L. Elias, D. Worrall, S.P. Spencer, R.G. Jackson, G. Taguchi, J. Ross, D.J. Bowles


Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.
Eur. J. Biochem.,268:4086-4094 2001(Aug.)
Author:G. Taguchi, T. Yazawa, N. Hayashida, M. Okazaki


Plant hormone regulation on scopoletin metabolism from culture medium into tobacco cells
Plant Science,160:905-911 2001
Author:Goro Taguchi, Kotarou Yoshizawa, Ritsuko Kodaira, Nobuaki Hayashida, Mitsuo Okazaki


Increases of secondary metabolite production in various plant cell cultures by co-cultivation with cork.
Biosci. Biotechnol. Biochem.,65:853-860 2001
Author:H. Yamamoto, A. Yato,K. Yazaki, H. Hayashi, G. Taguchi, K. Inoue


Scopoletin Uptake from Culture Medium and Accumulation in the Vacuoles after Conversion to Scopolin in 2,4-D-Treated Tobacco Cells.
Plant Science,151(2):153-161 2000
Author:Goro Taguchi, Shinobu Fujikawa, Teruyoshi Yazawa, Ritsuko Kodaira, Nobuaki Hayashida, Makoto Shimosaka, Mitsuo Okazaki


Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells
Plant Science,157(1):105-112 2000
Author:Goro Taguchi, Hirofumi Imura, Yoshio Maeda, Ritsuko Kodaira, Nobuaki Hayashida, Makoto Shimosaka, Mitsuo Okazaki


Effect of methyl jasmonate and elicitor on PAL gene expression in tobacco cultured cells.
J. Plant Biochem. Biotech.,7(2):79-84 1998
Author:Goro Taguchi, Manisha Sharan, Keiichi Gonda, Kuninori Yanagisawa, Makoto Shimosaka, Nobuaki Hayashida, Mitsuo Okazaki


Effects of methyl jasmonate and elicitor on the activation of phenylalanine ammonia-lyase and the accumulation of scopoletin and scopolin in tobacco cell cultures
Plant Science,132(1):13-19 1998
Author:Manisha Sharan, Goro Taguchi, Keiichi Gonda, Takashi Jouke, Makoto Shimosaka, Nobuaki Hayashida,Mitsuo Okazaki


A temperature-sensitive osmophilic mutant of Zygosaccharomyces rouxii
Biotechnol. Lett.,18(6):655-658 1996(Jun.)
Author:S. Yoshikawa, Y. Togawa, N. Mitsui, K. Chikara, G. Taguchi, M. Shimosaka, M. Okazaki


Cloning and characterization of mycovirus double-stranded RNA from the plant pathogenic fungus, Fusarium solani f. sp. robiniae.
Biosci. Biotech. Biochem.,60(5):784-788 1996
Author:M. Nogawa, T. Kageyama, A.Nakatani,G. Taguchi, M. Shimosaka, M. Okazaki


Cloning of theSAT1 gene concerned with salt tolerance of the yeast Zygosaccharomyces rouxii.
J. Ferment. Bioeng.,82(1):16-21 1996
Author:K. Ushio, H. Otsuka, S. Yoshikawa, G. Taguchi, M. Shimosaka, N. Mitsui, M. Okazaki


Transformation of tobacco cultured cell by particle bombardment : Expression of phenylalanine ammonia-lyase gene in transgenic tobacco cells.
Plant Tissue Culture Letters,12(2):165-171 1995
Author:Nobuo Nagai, Kuninori Yanagisawa, Kazuko Mizuno, Hirofumi Imura, Goro Taguchi, Makoto Shimosaka, Daisuke Shibata, Mitsuo Okazaki


Role of malic acid in solubilizing excess berberine accumulating in vacuoles ofCoptis japonica.
Phytochemistry,31:3451-3454 1992
Author:Hideharu Sato, Goro Taguchi, Hiroshi Fukui, Mamoru Tabata


学会発表
Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis;
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :IE-06 2013
Author:Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi


Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – Development of gene disruption by an allelic exchange system –
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :PE-01 2013
Author:Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka


Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – chiG, chiJ, and chiK coding for unique chitin-degrading enzymes –
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :PE-02 2013
Author:Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka:


Isolation of Genes Coding for Chitin-Degrading Enzymes in the Chitinolytic Bacterium, Chitiniphilus shinanonensis and Characterization of a Gene Coding for a Novel Chitinaese
6th International Conference on Advanced Fiber/Textile Materials , :IP-08 2011
Author:Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA


Analysis of genes coding for chitin-degrading enzymes from the bacterium Chitiniphilus shinanonensis
International Conference of Future Textile 2010 , :P-07 2010
Author:L. Huang, M. Nogawa, G.Taguchi, M. Shimosaka


An approach to improving plant productivity by use of a LED unit equipped with control modules.
6th International Symposium on Light in Horticulture. , :114 2009
Author:H. Nozue, A. Shimada, Y. Yabuuchi, T. Itoh, J. Kinoshita, H. Jibu, I. Umayahara, G. Taguchi, M. Nozue


Isolation and Characterization of a Novel Chitinolytic Bacterium, 
-Construction of Stable Chitinolytic Microbial Community-.
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009) , :PS01 2009
Author:K. Sato, H. Aoyama, Y. Kato, M. Nogawa, G. Taguchi, A. Yokota, M. Shimosaka


Degradation of chitin by a novel bacterium
Chitiniphilus shinanonensis originated from a bacterial community expressing a chitinolytic activity
5th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda. , :S1-P-28 2009
Author:K. Sato, A. Shizume, H. Aoyama, Y. Kato, M. Nogawa, G. Taguchi, M. Shimosaka


Analysis of Bacterial Consortium Growing on Chitin Flake by Using Metagenomic Approach
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007) , :142 2007
Author:K. Sato, Y. Azama, R. Kodaira, G. Taguchi, M. Shimosaka


その他
ソバのフラボノイドC-配糖化酵素の同定と利用
バイオインダストリー協会 , バイオサイエンスとインダストリー , 73(3):232−233 2015
Author:田口悟朗


切れない糖修飾を作る酵素 (総説)
生物工学会誌 , 93(1):36 2015
Author:田口悟朗


植物工場技術の研究・開発および実証・展示・教育拠点 (9)信州大学
植物環境工学 , 25(2):65−69 2013
Author:野末雅之,野末はつみ,宇佐美久尚,高橋伸英,田口悟朗,梶浦善太


植物の異物代謝における配糖化とマロニル化 (総説)
バイオサイエンスとインダストリー , 69:215-217 2011
Author:田口悟朗


植物の異物修飾機構を応用した配糖体生産への取り組み (総説)
生物工学会誌 , 89(11):643-645 2011
Author:田口悟朗

特許等知的財産
登録済特許
ヒカリゴケ培養方法
共同研究等希望テーマ
ヒカリゴケの増殖・保護と有効利用
酵素反応を利用した物質生産
研究費
科学研究費補助金(研究代表者)
2014 - 2016 , 植物のフラボノイドC配糖化酵素の反応機構の解明 , 基盤研究(C)
2011 - 2013 , シロイヌナズナのフェノール性異物配糖体の輸送機構の解析 , 基盤研究(C)
2004 - 2005 , タバコにおけるフェノール性異物の配糖化・マロニル化と解毒・蓄積機構の解析 , 若手研究(B)

科学研究費補助金(研究分担者)
2012 - 2015 , 基盤研究(C)
2007 - 2009 , エノキタケ子実体誘導に関与する遺伝子群の機能解析と遺伝子操作系の開発 , 基盤研究(C)

奨学寄附金
2007

その他公的資金
2001 - , タバコの配糖化酵素遺伝子群の機能解析

教育活動実績

授業等
2017 , 前期 , 応用生物学への招待 , (分担, 2012年~)
2017 , 前期 , 植物工学特論 , (2012年~)
2017 , 前期 , 天然物化学 , (2014年~)
2017 , 前期 , 生物機能科学実験Ⅰ , (2014年~)
2017 , 後期 , 有機化学2 , (2008年~)