田口 悟朗 (タグチ ゴロウ)
学術研究院（繊維学系）
繊維学部 応用生物科学科
教授
研究者情報
研究キーワード
- 植物の二次代謝, 酵素反応, 生合成, 配糖化, 異物代謝, ヒカリゴケ
メールアドレス
- gtagtag★shinshu-u.ac.jp
経歴
研究活動情報
論文
- A novel endo-type chitinase possessing chitobiase activity derived from the chitinolytic bacterium, Chitiniphilus shinanonensis SAY3T
Okazaki, S., Komatsu, A., Nakano, M., Taguchi, G., Shimosaka, M.
Bioscience, Biotechnology, and Biochemistry, 87(12), 1543-1550, 2023年12月, 査読有り
- Multi‐enzyme machinery for chitin degradation in the chitinolytic bacterium Chitiniphilus shinanonensis SAY3T
Sato, H., Sonoda, N., Nakano, M., Matsuyama, Y., Shizume, A., Arai, R., Nogawa, M., Taguchi, G., Shimosaka, M.
CURRENT MICROBIOLOGY, 80, 360, 2023年09月, 査読有り - Production of flavonoid 7-O-glucosides by bioconversion using Escherichia coli expressing a 7-O-glucosyltransferase from tobacco (Nicotiana tabacum)
Nasanjargal Dorjjugder, Goro Taguchi
Applied Biochemistry and Biotechnology, 194, 3320-3329, 2022年, 査読有り
最終著者, 責任著者 - Enzymatic basis for stepwise C-glycosylation in the formation of flavonoid di-C-glycosides in sacred lotus (Nelumbo nucifera Gaertn.)
Feng, C.Y., Li, S.-S., Taguchi, G., Wu, Q., Yin, D.-D., Gu, Z.-Y., Wu, J., Xu, W.-Z., Liu, C., Wang, L.-S.
Plant J, 106(2), 351-365, 2021年, 査読有り - Production of flavonol and flavone 6-C-glucosides by bioconversion in Escherichia coli expressing a C-glucosyltransferase from wasabi (Eutrema japonicum)
Nasanjargal Dorjjugder, Mayu Hatano, Goro Taguchi
Biotechnology Letters, 43(9), 1913-1919, 2021年, 査読有り
最終著者, 責任著者 - Biochemical characterization of the jasmonic acid methyltransferase gene from wasabi (Eutrema japonicum)
Koeduka,T., Suzuki, H., Taguchi, G., Matsui, K.
Plant Biotechnol., 37, 389-392, 2020年, 査読有り - Identification and characterization of apigenin 6-C-glucosyltransferase involved in biosynthesis of isosaponarin in wasabi (Eutrema japonicum).
Mashima, K, Hatano, M, Suzuki, H, Shimosaka, M, Taguchi G.
Plant Cell Physiol., 60(12), 2733-2743, 2019年, 査読有り
最終著者, 責任著者 - Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)
Koja, E., Ohata, S., Maruyama, Y., Suzuki, H., Shimosaka, M., Taguchi, G.
Biosci. Biotechnol. Biochem., 82(10), 1790-1802, 2018年, 査読有り
- C-Glycosyltransferases catalyzing the formation of di- C-glucosyl flavonoids in citrus plants.
Ito, T., Fujimoto, S., Suito, F., Shimosaka, M., Taguchi, G.
Plant J, 91(2), 187-198, 2017年, 査読有り - Taste characterization of the fruit body derived from a novel strain belonging to the genus Pleurotus
Yoshihito Azumi, Kahori Doi, Kana Kogiso, Goro Taguchi, Makoto Shimosaka, Kenji Ouchi, Satoshi Inatomi
Mushroom Sci Biotechnol, 24(3), 129-135, 2016年, 査読有り - pFungiway: a series of plasmid vectors used for gene manipulation in fungi.
Nishikawa, R., Yoshida, M.,Noda,T., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M.
Annals of Microbiology, 66(2), 825–832, 2016年 - Purification, molecular cloning and functional characterization of flavonoid-C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.
Plant J, 80(3), 437-448, 2014年, 査読有り - Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C- glucosyltransferases
Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.
Plant Biotechnol., 31(5), 519-524, 2014年 - Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes.
Sekiya, S., Yamada, M., Shibata, K., Okuhara, T., Yoshida, M., Inatomi, S., Taguchi, G., Shimosaka, M.
J. Biosci. Bioeng., 115(4), 360-365, 2013年 - Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold, Aspergillus oryzae
Akihiro Sugita, A., Sugii, A., Sato, K., Zhang, X.-Y., Dai, A.-L., Taguchi, G., Shimosaka, M
Biosci. Biotech. Biochem., 76, 193-195, 2012年 - Structural basis for modification of flavonol and naphthol glucoconjugates by Nicotiana tabacum malonyltransferase (NtMaT1).
Manjasetty, B.A., Yu, X.-H., Panjikar, S., Taguchi, G., Chance, M.R., Liu, C.-J.
Planta, 236, 781-793, 2012年, 査読有り - Contribution of CoA ligases to benzenoid biosynthesis in petunia flowers.
Klempien, A., Kaminaga, Y., Qualley, A., Nagegowda, D. A., Widhalm, J. R., Orlova, I., Kumar-Shasany, A., Taguchi, G., Kish, C.M., Cooper, B.R., D’Auria, J.C., Rhodes, D., Pichersky, E., Dudareva, N.
Plant Cell, 24, 2015-2030, 2012年 - Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium, Chitiniphilus shinanonensis
Huang L., Shizume, A., Nogawa, M., Taguchi, G., Shimosaka, M.
Biosci. Biotech. Biochem., 76, 517-522, 2012年 - Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase.
Huang L., Garbulewska, E., Sato, K., Kato , Y., Shizume, A., Nogawa, M., Taguchi, G., Shimosaka, M.
J. Biosci. Bioeng., 113, 293-299, 2011年02月12日 - Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of cultivated mushroomFlammulina velutipes
Okamoto, T., Yamada, M., Sekiya,S., Okuhara, T., Taguchi, G., Inatomi, S., Shimosaka, M.
Biosci. Biotech. Biochem., 74(11), 2327-2329, 2010年11月 - Malonylation is a key reaction in the metabolism of xenobiotic phenolic glucosides in Arabidopsis and tobacco
Taguchi, G., Ubukata,T., Nozue, H., Kobayashi, Y., Takahi, M., Yamamoto, H., Hayashida, N.
Plant J., 63(6), 1031-1041, 2010年09月 - Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Sato K., Azama Y., Nogawa M., Taguchi G., Shimosaka M.
J. Biosci. Bioeng., 109(5), 472-478, 2010年05月 - Construction and analysis of a bacterial community exhibiting a strong chitinolytic activity
Sato, K., Kato, Y., Fukamachi, A., Nogawa, M., Taguchi, G., Shimosaka M.
Biosci. Biotech. Biochem., 74(3), 636-640, 2010年03月 - Suppression of Arogenate Dehydratase1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals.
MaedaH, Shasany AK, Schnepp J, Orlova I, Taguchi G, Cooper BR, Rhodes D, Pichersky E, Dudareva N.
Plant Cell, 22(3), 832-849, 2010年03月 - Agrobacterium tumefaciens-mediated transformation of antifungal lipopeptide producing fungus Coleophoma empetri.
Yamada, M., Yawata, K., Orino, Y., Ueda, S., Isogai, Y., Taguchi, G. Shimosaka, M, Hashimoto,S.
Current Genetics, 55(6), 623-630, 2009年12月 - Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
Sato, K., Kato, Y.,Taguchi, G., Nogawa, M., Yokota, A., Shimosaka, M.
J. Gen. Appl. Microbiol., 55(2), 147-153, 2009年05月 - Construction of a Practical SCAR Marker Linked toClubroot Resistance in Chinese Cabbage, with Intensive Analysis of HC352b Genes
Hayashida, N., Takabatake, Y., Nakazawa, N., Aruga, D., Nakanishi, H., Taguchi, G., Sakamoto, K., Matsumoto, E.
J. Japan. Soc. Hort. Sci., 77(2), 150-154, 2008年04月 - Mapping of isolate-specific QTLs for clubroot resistance in Chinese cabbage (Brassica rapa L. ssp. pekinensis).
Sakamoto, K., Saito, A., Taguchi, G., Hayashida, N., Matsumoto, E.
Theor. Appl. Genet., 117, 759-767, 2008年 - Characterization of a gene conferring red fluorescence isolated from an environmental DNA library constructed from soil bacteria.
Lan, X.-Q., Sato, K. Taguchi, G., Zhou,Z.-Y., Shimoaka, M.
Biosci. Biotech. Biochem., 72, 1908-1914, 2008年 - Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
Yamada, M., Kurano, M., Inatomi, S., Taguchi, G., Okazaki, M., Shimosaka,M.
FEMS Microbiol Lett., 289, 130-137, 2008年 - Convergent evolution in the BAHD family of acyl transferases: identification and characterization of anthocyanin acyl transferases from Arabidopsis thaliana
Luo, J; Nishiyama, Y; Fuell, C; Taguchi, G; Elliott, K; Hill, L; Tanaka, Y; Kitayama, M; Yamazaki, M; Bailey, P; Parr, A; Michael, AJ; Saito, K;Martin, C
Plant J., 50(4), 678-695, 2007年 - Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomyceteFlammulina velutipesby fluorescence differential display
Masato Yamada;Sou Sakuraba;Kou Shibata;Goro Taguchi;Satoshi Inatomi;Mitsuo Okazaki;Makoto Shimosaka
FEMS Microbiol. Lett., 254(1), 165-172, 2006年 - Molecular cloning, characterization, and downregulation of an acyltransferase that catalyzes the malonylation of flavonoid and naphthol glucosides in tobacco cells.
Goro Taguchi, Yoshihiro Shitchi, Sakiko Shirasawa, Hirobumi Yamamoto, Nobuaki Hayashida
Plant J., 42, 481-491, 2005年05月 - Characterization of the Arabidopsis thaliana mutant pcb2 which accumulates divinyl chlorophylls.
Hiromitsu Nakanishi, Hatsumi Nozue, Kenji Suzuki, Yasuko Kaneko, Goro Taguchi, Nobuaki Hayashida
Plant Cell Physiol., 46(3), 467-473, 2005年03月 - Collection and analysis of Arabidopsis color mutants.
Hiromitsu Nakanishi, Kenji Suzuki, Takashi Jouke, Ritsuko Kodaira, Goro Taguchi, Mitsuo Okazaki, Nobuaki Hayashida
Endocytobiosis Cell Res, 15(1), 328-338, 2004年 - Metabolism of administered (2RS)-naringenin in flavonoidproducing cultured cells of Sophora flavescens
Hirobumi Yamamoto, Hiroki Kuribayashi, Yasuharu Seshima, Ping Zhao, Isao Kouno, Goro Taguchi, Koichiro Shimomura
Plant Biotech., 21(5), 355-359, 2004年 - Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.
Goro Taguchi, Takahisa Ubukata, Nobuaki Hayashida, Hirobumi Yamamoto, Mitsuo Okazaki
Arch. Biochem. Biophys., 420(1), 95-102, 2003年11月 - Evolution of substrate recognition across a multigene family of glycosyltransferases in Arabidopsis.
E.K. Lim, S. Baldauf, Y. Li, L. Elias, D. Worrall, S.P. Spencer, R.G. Jackson, G. Taguchi, J. Ross, D.J. Bowles
GLYCOBIOLOGY, 13, 139-145, 2003年 - Exogenously added naphthols induce three glucosyltransferases, and are accumulated as glucosides in tobacco cells.
Goro Taguchi, Minako Nakamura, Nobuaki Hayashida, Mitsuo Okazaki
Plant Science, 164, 231-240, 2003年 - Molecular cloning and heterologous expression of novel glucosyltransferases from tobacco cultured cells that have broad substrate specificity and are induced by salicylic acid and auxin.
G. Taguchi, T. Yazawa, N. Hayashida, M. Okazaki
Eur. J. Biochem., 268, 4086-4094, 2001年08月 - Increases of secondary metabolite production in various plant cell cultures by co-cultivation with cork.
H. Yamamoto, A. Yato,K. Yazaki, H. Hayashi, G. Taguchi, K. Inoue
Biosci. Biotechnol. Biochem., 65, 853-860, 2001年 - Plant hormone regulation on scopoletin metabolism from culture medium into tobacco cells
Goro Taguchi, Kotarou Yoshizawa, Ritsuko Kodaira, Nobuaki Hayashida, Mitsuo Okazaki
Plant Science, 160, 905-911, 2001年 - Scopoletin Uptake from Culture Medium and Accumulation in the Vacuoles after Conversion to Scopolin in 2,4-D-Treated Tobacco Cells.
Goro Taguchi, Shinobu Fujikawa, Teruyoshi Yazawa, Ritsuko Kodaira, Nobuaki Hayashida, Makoto Shimosaka, Mitsuo Okazaki
Plant Science, 151(2), 153-161, 2000年 - Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells
Goro Taguchi, Hirofumi Imura, Yoshio Maeda, Ritsuko Kodaira, Nobuaki Hayashida, Makoto Shimosaka, Mitsuo Okazaki
Plant Science, 157(1), 105-112, 2000年 - Effects of methyl jasmonate and elicitor on the activation of phenylalanine ammonia-lyase and the accumulation of scopoletin and scopolin in tobacco cell cultures
Manisha Sharan, Goro Taguchi, Keiichi Gonda, Takashi Jouke, Makoto Shimosaka, Nobuaki Hayashida,Mitsuo Okazaki
Plant Science, 132(1), 13-19, 1998年 - Effect of methyl jasmonate and elicitor on PAL gene expression in tobacco cultured cells.
Goro Taguchi, Manisha Sharan, Keiichi Gonda, Kuninori Yanagisawa, Makoto Shimosaka, Nobuaki Hayashida, Mitsuo Okazaki
J. Plant Biochem. Biotech., 7(2), 79-84, 1998年 - A temperature-sensitive osmophilic mutant of Zygosaccharomyces rouxii
S. Yoshikawa, Y. Togawa, N. Mitsui, K. Chikara, G. Taguchi, M. Shimosaka, M. Okazaki
Biotechnol. Lett., 18(6), 655-658, 1996年06月 - Cloning of theSAT1 gene concerned with salt tolerance of the yeast Zygosaccharomyces rouxii.
K. Ushio, H. Otsuka, S. Yoshikawa, G. Taguchi, M. Shimosaka, N. Mitsui, M. Okazaki
J. Ferment. Bioeng., 82(1), 16-21, 1996年 - Cloning and characterization of mycovirus double-stranded RNA from the plant pathogenic fungus, Fusarium solani f. sp. robiniae.
M. Nogawa, T. Kageyama, A.Nakatani,G. Taguchi, M. Shimosaka, M. Okazaki
Biosci. Biotech. Biochem., 60(5), 784-788, 1996年 - Transformation of tobacco cultured cell by particle bombardment : Expression of phenylalanine ammonia-lyase gene in transgenic tobacco cells.
Nobuo Nagai, Kuninori Yanagisawa, Kazuko Mizuno, Hirofumi Imura, Goro Taguchi, Makoto Shimosaka, Daisuke Shibata, Mitsuo Okazaki
Plant Tissue Culture Letters, 12(2), 165-171, 1995年 - Role of malic acid in solubilizing excess berberine accumulating in vacuoles ofCoptis japonica.
Hideharu Sato, Goro Taguchi, Hiroshi Fukui, Mamoru Tabata
Phytochemistry, 31, 3451-3454, 1992年
MISC
- 切れない糖修飾を作る酵素 （総説）
田口悟朗
生物工学会誌, 93(1), 36, 2015年 - ソバのフラボノイドC-配糖化酵素の同定と利用
田口悟朗
バイオサイエンスとインダストリー, 73(3), 232−233, 2015年 - 植物工場技術の研究・開発および実証・展示・教育拠点 （9）信州大学
野末雅之，野末はつみ，宇佐美久尚，高橋伸英，田口悟朗，梶浦善太
植物環境工学, 25(2), 65−69, 2013年 - 植物の異物代謝における配糖化とマロニル化 （総説）
田口悟朗
バイオサイエンスとインダストリー, 69, 215-217, 2011年 - 植物の異物修飾機構を応用した配糖体生産への取り組み （総説）
田口悟朗
生物工学会誌, 89(11), 643-645, 2011年
書籍等出版物
- 水谷正治、士反伸和、杉山曉史編 『基礎から学ぶ植物代謝生化学』
飯島陽子，田口悟朗, 共著, 『第１７章 食品成分・薬用成分・毒』
羊土社, 283-300 2018年12月 - Molecular Breeding and Nutritional Aspects of Buckwheat (Zhou et al. Eds)
Goro Taguchi, Flavonoid Biosynthesis in Buckwheat. (Chapter 30)
Academic Press (Elsevier), 377-386 2016年 - 濱田博喜監修『機能性配糖体の合成と応用−糖転移酵素を中心に−』
田口悟朗, 植物の異物修飾機構を応用した配糖体生産法.
シーエムシー出版, 5-15 2013年 - 生物工学実験書改訂版
日本生物工学会編 （岡崎光雄，田口悟朗）, 共著
培風館, 164-172 2002年04月 - Recent Res. Devel. Biotech.&Bioeng. 2
H. Yamamoto, K. Yazaki, H. Hayashi, G. Taguchi, A. Yato, K. Inoue, 共著, Effect of cork tissues on the production of secondary metabolites in various plantcell cultures.
273-281 1999年
講演・口頭発表等
- Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – chiG, chiJ, and chiK coding for unique chitin-degrading enzymes –
Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka:
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – Development of gene disruption by an allelic exchange system –
Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis;
Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Isolation of Genes Coding for Chitin-Degrading Enzymes in the Chitinolytic Bacterium, Chitiniphilus shinanonensis and Characterization of a Gene Coding for a Novel Chitinaese
Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA
6th International Conference on Advanced Fiber/Textile Materials, 2011年 - Analysis of genes coding for chitin-degrading enzymes from the bacterium Chitiniphilus shinanonensis
L. Huang, M. Nogawa, G.Taguchi, M. Shimosaka
International Conference of Future Textile 2010, 2010年 - Degradation of chitin by a novel bacterium Chitiniphilus shinanonensis originated from a bacterial community expressing a chitinolytic activity
K. Sato, A. Shizume, H. Aoyama, Y. Kato, M. Nogawa, G. Taguchi, M. Shimosaka
5th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda., 2009年 - Isolation and Characterization of a Novel Chitinolytic Bacterium, -Construction of Stable Chitinolytic Microbial Community-.
K. Sato, H. Aoyama, Y. Kato, M. Nogawa, G. Taguchi, A. Yokota, M. Shimosaka
7th East Asian Symposium on Polymers for Advanced Technologies (EASPAT 2009), 2009年 - An approach to improving plant productivity by use of a LED unit equipped with control modules.
H. Nozue, A. Shimada, Y. Yabuuchi, T. Itoh, J. Kinoshita, H. Jibu, I. Umayahara, G. Taguchi, M. Nozue
6th International Symposium on Light in Horticulture., 2009年 - Analysis of Bacterial Consortium Growing on Chitin Flake by Using Metagenomic Approach
K. Sato, Y. Azama, R. Kodaira, G. Taguchi, M. Shimosaka
4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda (ICAFTM2007), 2007年
担当経験のある科目_授業
共同研究・競争的資金等の研究課題
- タバコにおけるフェノール性異物の配糖化・マロニル化と解毒・蓄積機構の解析
科学研究費補助金, 若手研究(B)
2004年 - タバコの配糖化酵素遺伝子群の機能解析
2001年 - 異なる反応性を持つフラボノイドＣ−配糖化酵素の反応メカニズムの解明
科学研究費補助金, 基盤研究(C) - 植物のフラボノイドC配糖化酵素の反応機構の解明
科学研究費補助金, 基盤研究(C) - シロイヌナズナのフェノール性異物配糖体の輸送機構の解析
科学研究費補助金, 基盤研究(C) - 新規キチン分解酵素を用いたキチン質バイオマスからの有用糖質素材の生産
科学研究費補助金, 基盤研究(C) - エノキタケ子実体誘導に関与する遺伝子群の機能解析と遺伝子操作系の開発
科学研究費補助金, 基盤研究（C)
社会活動実績
