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海老沼宏安  エビヌマ ヒロヤス

教員組織学術研究院(繊維学系)電話番号
教育組織繊維学部 応用生物科学科FAX番号
職名教授メールアドレス
住所〒386-8567 長野県上田市常田3-15-1ホームページURL

研究活動業績

研究業績(著書・
発表論文等)
著書
遺伝子組換えユーカリ(4章)
海青社 , 早生樹: 産業植林とその利用 , :123-128 2012
Author:河岡明義、松永悦子、海老沼宏安、島田照久


Marker-free gene targeting by recombinase-mediated cassette exchange (RMCE).
Springer , Methods in Molecular Biology 847, Transgenic Plants 2nd Edition. , :379-390 2012
Author:Ebinuma, H., Nanto, K. Kasahara, S. and Komamine, A.


組換え技術の信頼性向上
海青社 , 森をとりもどすにため②林木の育種 , :107-117 2010
Author:海老沼宏安


Marker-free targeted transformation
Springer , Molecular Techniques in Crop Improvement 2nd Edition , :527-543 2009
Author:Ebinuma, H. and Nanto, K.


論文
Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange
MOLECULAR BREEDING,35(2):82 2015(Feb.)
Author:Ebinuma, H; Nakahama, K; Nanto, K


A field trial to assess the environmental biosafety of codA-transgenic Eucalyptus camaldulensis cultivation
Plant Biotechnology,30:357–363 2013
Author:Yu, X., Kikuchi, A., Matsunaga, E., Kawaoka, A., Ebinuma, H., Watanabe, K.


Agrobacterium-mediated transformation of Eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance.
Plant cell reports,31:225-235 2012
Author:Matsunaga, E., Nanto, K., Oishi, M., Ebinuma, H., Morishita, Y., Sakurai, N., Suzuki, H., Shibata, D., and Shimada, T.


Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonene synthase cDNA
Plant biotechnology journal,8(1):28-37 2009
Author:Ohara, K., Matsunaga, E., Nanto, K., Yamamoto, K., Sasaki, K., Ebinuma, H., Yazaki, K.


26-week oral safety study in macaques for transgenic rice containing major human T-cell epitope peptides from Japanese Cedar pollen allergens
Journal of Agricultural and Food Chemistry,57:5633-5638 2009
Author:Domon, E., Takagi, H., Hirose, S., Sugita, K., Kasahara, S., Ebinuma, H., Takaiwa, F.


Allelopathy assessments for the environmental biosafety of the salt-tolerant transgenic Eucalyptus camaldulensis, genotype codA 12-5B, codA 12-5C, and codA 20C
Journal of Wood Science,55:149-153 2009
Author:Kikuchi, A., Yu, X., Shimazaki, T., Kawaoka, A., Ebinuma, H., Watanabe, K.


Expression of a transgene exchanged by the recombinase-mediated cassette exchange (RMCE) method in plants
Plant Cell Reports,28:777-785 2009
Author:Nanto K, Sato K, Katayama Y., Ebinuma, H.


Marker-free site-specific integration plants
Transgenic Research,17:337-344 2008
Author:Nanto, K., Ebinuma, H.


Reduction of lignin content by suppression of expression of the LIM domain transcription factor in Eucalyptus camaldulensis
Silvae Genetica,55(6):269-277 2006
Author:Kawaoka, A., Nanto, K., Ishii, K., Ebinuma, H.


Production of trans-10, cis-12 Conjugated Linoleic Acid in Rice
Transgenic Research,15(1):95-100 2006
Author:Kohno-Murase, J., Iwabuchi, M. Endo-Kasahara, S., Sugita, K., Ebinuma, H., Imamura, J.


Genetically modified rice seeds accumulating GLP-1 analogue stimulate insulin secretion from a mouse pancreatic beta-cell line
FEBS Letters,579(5):1085-1088 2005
Author:Sugita, K., Endo-Kasahara, S., Tada, Y., Lijun, Y., Yasuda, H., Hayashi, Y., Jomori, T., Ebinuma, H., Takaiwa F.


Agrobacterium-mediated RMCE approach for gene replacement
Plant Biotechnology Journal,3:203-214 2005
Author:Nanto, K. Yamada-Watanabe, K., Ebinuma, H.


Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid aspen
Plant Physiology,132:1177-1185 2003
Author:Kawaoka, A., Matsunaga, E., Endo, S., Kondo, S., Yoshida, K., Shinmyo, A., Ebinuma, H.


The transposon Tip100 from the common morning glory is an autonomous element that can transpose in tobacco plants
Molecular Genetics and Genomics,26:732-739 2002
Author:Ishikawa, N., Johzuka-Hisatomi, Y., Sugita, K., Ebinuma, H., Iida, S.


Single-step transformation for generating marker-free transgenic rice using the ipt-type MAT vector system
The Plant Journal,30:115-122 2002
Author:Endo, S., Sugita, K., Sakai, M., Tanaka, H., Ebinuma, H.


A new GST-MAT vector containing both the ipt gene and the iaaM/H genes can produce marker-free transgenic plants with high frequency
Plant Cell Reports,20:923-928 2002
Author:Endo, S., Kasahara, T., Sugita, K., Ebinuma, H.


Asexual production of selectable marker-free transgenic woody plants, vegetatively propagated species
Molecular Breeding,10:95-106 2002
Author:Matsunaga, E., Sugita, K., Ebinuma, H.


Ntlim1, a PAL-box binding factor, controls promoter activity of the horseradish wound-inducible peroxidase gene
Plant Molecular Biology,49(6):591-599 2002
Author:Kaothien, P., Kawaoka, A., Ebinuma, H., Yoshida, K., Shinmyo, A.


Transcriptional control of lignin biosynthesis by tobacco LIM protein
Phytochemistry,57(7):1149-1157 2001
Author:Kawaoka, A., Ebinuma, H.


The isopentenyl transferase gene is effective as a selectable marker gene for plant transformation in tobacco (Nicotiana tabacum cv. Petite Havana SRI)
Plant Cell Reports,20:60-66 2001
Author:Endo, S., Kasahara, T., Sugita, K., Matsunaga, E., Ebinuma, H.


Transgene stacking in plants in the absence of sexual crossing
Molecular Breeding,6:529-536 2000
Author:Sugita, K., Matsunaga, E., Kasahara, T., Ebinuma, H.


A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency
The Plant Journal,22(5):461-469 2000
Author:Sugita, K., Kasahara, T., Matsunaga, E., Ebinuma, H.


Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis
The Plant Journal,22(4):1-13 2000
Author:Kawaoka, A., Kaothien, P., Yoshida, K., Endo, S., Yamada, K., Ebinuma, H.


Effective selection system for generating marker-free transgenic plants independent of sexual crossing
Plant Cell Reports,18:941-947 1999
Author:Sugita, K., Matsunaga, E., Ebinuma, H.


Compartmentalized expression of two structurally and functionally distinct 4-coumarate: CoA ligase genes in aspen (Populus tremuloides)
Proceedings of the National Academy of Sciences of the United States of America,95:5407-5412 1998
Author:Hu, W-J., Kawaoka, A., Tsai, C-J., Lung, J., Osakabe, K., Ebinuma, H., Chiang, V.L.


A novel glycine-rich/hydrophobic 16 kDa polypeptide gene from tobacco: similarity to proline-rich protein genes and its wound-inducible and developmentally regulated expression
Plant Molecular Biology,33:667–678 1997
Author:Yasuda, E., Ebinuma, H., Wabiko, H.


Selection of marker-free transgenic plants using the isopentenyl transferase gene as a selectable marker
Proceedings of the National Academy of Sciences of the United States of America,94:2117-2121 1997
Author:Ebinuma, H., Sugita, K., Matsunaga, E., Yamakado, M.


The detection of mosaics and polyploids in a hereditary mosaic strain of the silk moth, Bombyx mori, using egg colour mutants
Genetics Research,51:223-229 1988
Author:Ebinuma, H., Kobayashi, M., Kobayashi, J., Shimada, T., Yoshitake, N.


Nucleotide sequence of a rice glutelin gene
FEBS Letters,221(1):43-47 1987
Author:Takaiwa, F, Ebinuma, H, Kikuchi, S. Oono, K.


Selective recombination system in Bombyx mori. I. Chromosome specificity of the modification effect
Genetics,117:521-531 1987
Author:Ebinuma, H.


Expression of homeotic genes in Bombyx mori estimated from asymmetry of dorsal closure in mutant/normal mosaics
Journal of Experimental Zoology,240(3):335-342 1986
Author:Shimada, T., Ebinuma, H., Kobayashi, M.


The genetic system controlling recombination in the silkworm
Genetics,99:231-245 1981
Author:Ebinuma, H., Yoshitake, N.