信州大学HOMEENGLISH交通・キャンパス案内

研究者総覧研究者総覧

研究者、研究内容などで検索
項目別検索はこちら

水野正浩  ミズノ マサヒロ

教員組織学術研究院(工学系)電話番号
教育組織工学部 物質化学科FAX番号
職名准教授メールアドレスm-mizuno(at)shinshu-u.ac.jp
住所〒380-8553 長野県長野市若里4丁目17-1ホームページURLhttp://wwweng.cs.shinshu-u.ac.jp/CHEM/lab_biochem/

プロフィール

研究分野
応用生物化学
木質科学
現在の研究課題
糸状菌が生産するセルロース分解酵素に関する研究

酢酸菌におけるバクテリアセルロースの生合成機構に関する研究

イオン液体によるセルロースの溶解と再生セルロースの酵素分解に関する研究

所属学会
所属学会
日本応用糖質科学会
日本農芸化学会
セルロース学会
学歴
出身大学院
2005 , 東京農工大学 , 連合農学研究科 , 生物工学専攻
2002 , 東京農工大学 , 農学研究科 , 応用生命化学専攻

出身学校・専攻等(大学院を除く)
2000 , 東京農工大学 , 農学部 , 応用生物科学科

取得学位
博士(農学) , 東京農工大学
受賞学術賞
2016 , 日本応用糖質科学会 奨励賞
研究職歴等
研究職歴
2017- , 信州大学 工学部 准教授
2007-2017 , 信州大学 工学部 助教
2006-2007 , 信州大学 工学部 助手
2005-2005 , 日本学術振興会特別研究員

研究活動業績

研究業績(著書・
発表論文等)
著書
第3章 酵素処理による食品残渣の有効利用
大学教育出版 , 応用細胞資源利用学 第2巻 2015(Dec. 20)
Author:水野正浩


第4章 種々の細菌によるセルロース生産
シーエムシー出版 , 機能性セルロース次元材料の開発と応用
Author:天野良彦,水野正浩


論文
Multimethod approach to understand the assembly of cellulose fibrils in the biosynthesis of bacterial cellulose
Cellulose,25(5):2771-2783 2018(May)
Author:Paavo A. Penttilä, Tomoya ImaiMarie CapronMasahiro MizunoYoshihiko AmanoRalf SchweinsJunji Sugiyama


Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing α-amylase TVA I
Amylase,2:1-10 2018(Mar.)
Author:Takashi Tonozuka, Takanori Nihira, Masahiro Mizuno, Atsushi Nishikawa, Shigehiro Kamitori


Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability
Bioscience, Biotechnology, and Biochemistry,79(5):738-746 2015(Jan. 03)
Author:Hiroto Nishijima, Kouichi Nozaki, Masahiro Mizuno, Tsutomu Arai, Yoshihiko Amano


Structure of regenerated celluloses treated with ionic liquids and comparison of their enzymatic digestibility by purified cellulase components
Australian Journal of Chemistry,65(11):1491-1496 2012(Oct. 29)
Author:Masahiro Mizuno, Shuji Kachi, Eiji Togawa, Noriko Hayashi, Kouichi Nozaki, Toshiyuki Itoh, Yoshihiko Amano


Creation of bacterial cellulose-fabric complexed material
Sen'i Gakkaishi,68(2):42-47 2012(Mar.)
Author:Masahiro Mizuno, Yousuke Kamiya, Tomoka Katsuta, Naohisa Oshima, Kouichi Nozaki, Yoshihiko Amano


Design of cellulose dissolving ionic liquids inspired by nature
ChemSusChem,5(2):388-391 2012
Author:Kazutaka Ohira, Yoshikazu Abe, Motoi Kawatsura, Kazuhiko Suzuki, Masahiro Mizuno, Yoshihiko Amano, Toshiyuki Itoh


Comparative study on the ability to produce gentiobiose in cellulose-producing bacteria Asaia bogorensis and Gluconacetobacter xylinus
Journal of Applied Glycoscience,58(4):147-150 2011
Author:Akio Kumagai, Masahiro Mizuno, Kouichi Nozaki, Inder M. Saxena, Yoshihiko Amano


Regulation of adsorption behavior of carbohydrate-binding module family 1 and endo-β-1,4-glucanase onto crystalline cellulose
Journal of Applied Glycoscience,58(4):133-138 2011
Author:Kouichi Nozaki, Hiroto Nishijima, Tsutomu Arai, Masahiro Mizuno, Nobuaki Sato, Yoshihiko Amano


Ultrafine cellulose fibers produced by Asaia bogorensis, an acetic acid bacterium.
Biomacromolecules,12(7):2815-2821 2011
Author:Akio Kumagai, Masahiro Mizuno, Naoto Kato, Kouichi Nozaki, Eiji Togawa, Shigeru Yamanaka, Kazuo Okuda, Inder M. Saxena, Yoshihiko Amano


Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94(T), and insight into the unusual catalytic mechanism.
Marine biotechnology,13(3):411-422 2011
Author:Yuji Hatada, Masahiro Mizuno, Zhijun Li, Yukari Ohta


Improvement in the productivity of xylooligosaccharides from waste medium after mushroom cultivation by hydrothermal treatment with suitable pretreatment.
Bioresource Technology,101(15):6006-6011 2010
Author:Sato, N., Shinji, K., Mizuno, M., Nozaki, K., Suzuki, M., Makishima, S., Shiroishi, M., Onoda, T., Takahashi, F., Kanda, K., Amano, Y.


Enzymatic production of glucosylxylose using a cellobiose phosphorylase-yeast combined system
Journal of Applied Glycoscience,58(1):31-34 2010
Author:Akio Kumagai, Sawaki Tada, Kouichi Nozaki, Masahiro Mizuno, Takahisa Kanda, Satoshi Suzuki, Kenichi Kusumoto, Takashi Sasaki, Yutaka Kashiwagi, Yoshihiko Amano


Development of continuous flow type hydrothermal reactor for hemicellulose fraction recovery from corncob.
Bioresource Technology,100:2842-2848 2009
Author:Makishima, S., Mizuno, M., Sato, N., Shinji, K., Suzuki, M., Nozaki, K., Takahashi, F., Kanda, T., Amano, Y.


Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49.
Journal of Molecular Biology,376:210-220 2008
Author:Mizuno, M., Koide, A., Yamamura, A., Akeboshi, H., Yoshida, H., Kamitori, S., Sakano, Y., Nishikawa, A., Tonozuka, T.


Screening and investigation of dye decolorization activities of basidiomycetes
J. Biosci. Bioeng.,105(1):69-72 2008
Author:Nozaki, K., Hai Beh, C., Masahiro Mizuno, M., Isobe, T., Shiroishi, M., Kanda, T., Amano, Y.


Structure and characteristics of an endo-β-1,4-glucanase, isolated from Trametes hirsuta, with high degradation to crystalline cellulose.
Biosci Biotechnol. Biochem.,71:2375-2382 2007
Author:Nozaki, K., Seki, T., Matsui, K., Mizuno, M., Kanda, T., Amano, Y.


Structural analysis of the water-soluble carbohydrate from Asaia bogorensis by NMR spectroscopy
J. Appl. Glycosci.,54:231-233 2007
Author:Kato, N., Mizuno, M., Nakai, Y., Nozaki, K., Suga, H., Kanda, T., Yamanaka, S., Amano, Y.


Viability and cellulose synthesizing ability of Gluconacetobacter xylinus cells under high-hydrostatic pressure.
Extremophiles,11:693-698 2007
Author:Kato, N., Sato, T., Kato, C., Yajima, M., Sugiyama, J., Kanda, T., Mizuno, M., Nozaki, K., Yamanaka, S., Amano, Y.


Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein.
FEBS J.,274:2109-2120 2007
Author:Tonozuka, T., Sogawa, A., Yamada, M., Matsumoto, N., Yoshida, H., Kamitori, S., Ichikawa, K., Mizuno, M., Nishikawa, A., Sakano, Y.


Recovery of soluble sugars from waste medium for Enokitake (Flammulina velutipes) mushroom cultivation with hydrothermal reaction and enzyme digestion
J. Appl. Glycosci.,53:261-266 2006
Author:Makishima, S., Nozaki, K., Mizuno, M., Netsu, E., Shinji, K., Shibayama, T., Kanda, T., Amano, Y.


Structure of a complex of Thermoactinomyces vulgaris R-47 α-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft.
Carbohydrate Research,341:1041-1046 2006
Author:Ohtaki, A., Mizuno, M., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.


Three-dimensional structure of glucodextranase, a glycoside hydrolase family 15 enzyme.
Biologia Bratislava,60:171-176 2005
Author:Mizuno, M., Tonozuka, T., Ichikawa, T., Kamitori, S., Nishikawa, A., Sakano, Y.


X-ray crystallographic study of glucodextranase from a Gram-positive bacterium, Arthrobacter globiformis I42.
J. Appl. Glycosci.,52:145-151 2005
Author:Mizuno, M., Tonozuka, T., Ichikawa, K., Kamitori, S., Nishikawa, A., Sakano, Y.


Bacterial and archaeal enzymes homologous to glucoamylase: characterization and subsite affinities of a glucoamylase from Thermoactinomyces vulgaris R-47.
Biologia Bratislava,60:161-165 2005
Author:Ichikawa, K., Tonozuka, T., Uotsu-Tomita, R., Mizuno, M., Nishikawa, A., Sakano, Y.


Kinetic studies of site-directed mutational isomalto-dextranase-catalyzed hydrolytic reactions on a 27 MHz quartz-crystal microbalance.
Biochemistry,44:9456-9461 2005
Author:Nihira, T., Mizuno, M., Tonozuka, T., Sakano, Y., Mori, T., Okahata, Y.


Site-directed mutagenesis of Tryptophan 622 of Thermoactinomyces vulgaris R-47 glucoamylase: pH optima and activities of five mutants.
J. Appl. Glycosci.,52:277-279 2005
Author:Ichikawa, K., Tonozuka, T., Mizuno, M., Nishikawa, A., Sakano, Y.


Mutagenesis and structural analysis of Thermoactinomyces vulgaris R-47 α-amylase II (TVA II).
J. Appl. Glycosci.,52:225-231 2005
Author:Mizuno, M., Ichikawa, K., Tonozuka, T., Ohtaki, A., Shimura, Y., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase.
Acta Crystallographica Sec. F,61:302-304 2005
Author:Ichikawa, K., Tonozuka, T., Mizuno, M., Tanabe, Y., Kamitori, S., Nishikawa, A., Sakano, Y.


Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition.
J. Biol. Chem.,279:31033-31040 2004
Author:Ohtaki, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.


The crystal structure of Thermoactinomyces vulgaris R-47 α-amylase II (TVA II) complexed with transglycosylated product.
Eur. J. Biochem.,271:2530-2538 2004
Author:Mizuno, M., Tonozuka, T., Uechi, A., Ohtaki, A., Ichikawa, K., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray analysis of Escherichia coli K12 Ygjk protein, a member of glycosyl hydrolase family 63.
Acta Crystallographica Sec. D,60:1284-1285 2004
Author:Tonozuka, T., Uechi, A., Mizuno, M., Ichikawa, K., Nishikawa, A., Sakano, Y.


Structural insights into substrate specificity and function of glucodextranase.
J. Biol. Chem.,279:10575-10583 2004
Author:Mizuno, M., Tonozuka, T., Suzuki, S., Uotsu-Tomita, R., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray study of isomaltodextranase from Arthrobacter globiformis.
Acta Crystallographica Sec. D,60:572-573 2004
Author:Akita, M., Mizuno, M., Tonozuka, T., Sakano, Y., Matsui, H., Hidaka, Y., Hatada, Y., Ito, S., Horikoshi, K.


Heterologous production and characterization of Arthrobacter globiformis T6 Isomalto-dextranase.
J. Appl. Glycosci.,51:27-32 2004
Author:Tonozuka, T., Suzuki, S., Ikehara, Y., Mizuno, M., Kim, YK., Nishikawa, A., Sakano, Y.


Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 α-amylases TVA I and TVA II by site-directed mutagenesis.
Carbohydrate Research,388:1553-1558 2003
Author:Ohtaki, A., Iguchi, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.


Crystal structures and substrate specificities of two α-amylases hydrolyzing cyclodextrins and pullulan from Thermoactinomyces vulgaris R-47
Biologia Bratislava,57:71-76 2002
Author:Tonozuka, T., Yokota, T., Ichikawa, K., Mizuno, M., Kondo, S., Nishikawa, A., Kamitori, S., Sakano, Y.