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水野 正浩  ミズノ マサヒロ

教員組織学術研究院(工学系)電話番号
教育組織工学部 物質化学科FAX番号
職名准教授メールアドレスm-mizuno(at)shinshu-u.ac.jp
住所〒380-8553 長野県長野市若里4丁目17-1ホームページURLhttp://www.shinshu-u.ac.jp/faculty/engineering/chair/chem007/index.html

プロフィール

研究分野
応用生物化学
木質科学
現在の研究課題
糸状菌が生産するセルロース分解酵素に関する研究

酢酸菌におけるバクテリアセルロースの生合成機構に関する研究

イオン液体によるセルロースの溶解と再生セルロースの酵素分解に関する研究

所属学会
所属学会
日本応用糖質科学会
日本農芸化学会
セルロース学会
学歴
出身大学院
2005 , 東京農工大学 , 連合農学研究科 , 生物工学専攻
2002 , 東京農工大学 , 農学研究科 , 応用生命化学専攻

出身学校・専攻等(大学院を除く)
2000 , 東京農工大学 , 農学部 , 応用生物科学科

取得学位
博士(農学) , 東京農工大学
受賞学術賞
2016 , 日本応用糖質科学会 奨励賞
研究職歴等
研究職歴
2017- , 信州大学 工学部 准教授
2007-2017 , 信州大学 工学部 助教
2006-2007 , 信州大学 工学部 助手
2005-2005 , 日本学術振興会特別研究員

研究活動業績

研究業績(著書・
発表論文等)
書籍等出版物
第3章 酵素処理による食品残渣の有効利用, 応用細胞資源利用学 第2巻
大学教育出版 2015(Dec. 20)
Author:水野正浩


バイオマス分解酵素研究の最前線-セルラーゼ・ヘミセルラーゼを中心として-, 259-264
シーエムシー出版 2012(Mar.)
Author:近藤昭彦,天野良彦,田丸浩 監修


第4章 種々の細菌によるセルロース生産, 機能性セルロース次元材料の開発と応用
シーエムシー出版
Author:天野良彦,水野正浩


論文
Xylanase from marine filamentous fungus Pestalotiopsis sp. AN-7 was activated with diluted salt solution like brackish water
Journal of Applied Glycoscience,68(1):11-18 2021(Mar. 11)
Author:Sangho Koh, Masahiro Mizuno, Yuto Izuoka, Naoto Fujino, Naoko Hamada-Sato, Yoshihiko Amano


Photoelectrochemical complete decomposition of cellulose for electric power generation
ChemCatChem,https://doi.org/10.1002/cctc.202001665 2020(Dec. 14)
Author:Yosuke Kagoshima, Takumi Yoshimura, Sangho Koh, Masahiro Mizuno, Katsuya Teshima, Hiromasa Nishikiori


Growth of dispersed hydroxyapatite crystals highly intertwined with TEMPO-oxidized cellulose nanofiber
CrystEngComm,22:4933-4941 2020(Jul. 06)
Author:Tetsuya Yamada, Takeo Kitamura, Yuko Morita, Masahiro Mizuno, Kunio Yubuta, Katsuya Teshima


高標高地域で生産された「山恵錦」の玄米品質,加工適性および麴製造適性
北陸作物学会報,55:4-6 2020(Jun. 01)
Author:細井淳,豊田敦至,水野正浩,岩下和裕,岩野君夫


イオン液体を用いた天然型キシランの抽出とその構造的特徴
応用糖質科学,10(2):76-82 2020(May)
Author:高相昊, 水野正浩, 河本啓太, 三森亮, 早川源矢, 天野良彦


Characterization of acetylxylan esterase from white-rot fungus Irpex lacteus
Journal of Applied Glycoscience,66(4):131-137 2019(Nov.)
Author:Sangho Koh, Seika Imamura, Naoto Fujino, Masahiro Mizuno, Nobuaki Sato, Satoshi Makishima, Peter Biely, Yoshihiko Amano


Structural characterization of hemicellulose released from corn cob in continuous flow type hydrothermal reactor
Journal of Bioscience and Bioengineering,127(2):222-230 2019(Feb.)
Author:TsutomuArai, Peter Biely, Iveta Uhliariková, Nobuaki Sato, Satoshi Makishima, Masahiro Mizuno, Kouichi Nozaki, Satoshi Kaneko, Yoshihiko Amano


Multimethod approach to understand the assembly of cellulose fibrils in the biosynthesis of bacterial cellulose
Cellulose,25(5):2771-2783 2018(May)
Author:Paavo A. Penttilä, Tomoya ImaiMarie CapronMasahiro MizunoYoshihiko AmanoRalf SchweinsJunji Sugiyama


Mutagenesis-induced conformational change in domain B of a pullulan-hydrolyzing α-amylase TVA I
Amylase,2:1-10 2018(Mar.)
Author:Takashi Tonozuka, Takanori Nihira, Masahiro Mizuno, Atsushi Nishikawa, Shigehiro Kamitori


セルロースの高次構造改変とその酵素分解性の評価
応用糖質科学,7(1):10-15 2017(Feb.)
Author:水野正浩


Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability
Bioscience, Biotechnology, and Biochemistry,79(5):738-746 2015(Jan. 03)
Author:Hiroto Nishijima, Kouichi Nozaki, Masahiro Mizuno, Tsutomu Arai, Yoshihiko Amano


Irpex lacteus由来GH10キシラナーゼの新規セルロース高吸着CBM1
応用糖質科学,4(2):154-159 2014(May 20)
Author:西島拓人,野﨑功一,水野正浩,荒井勉,天野良彦


Irpex lacteus由来GH10キシラナーゼの新規セルロース高吸着CBM1
応用糖質科学,4(2):154-159 2014(May)
Author:西島拓人,野﨑功一,水野正浩,荒井勉,天野良彦


Structure of regenerated celluloses treated with ionic liquids and comparison of their enzymatic digestibility by purified cellulase components
Australian Journal of Chemistry,65(11):1491-1496 2012(Oct. 29)
Author:Masahiro Mizuno, Shuji Kachi, Eiji Togawa, Noriko Hayashi, Kouichi Nozaki, Toshiyuki Itoh, Yoshihiko Amano


Creation of bacterial cellulose-fabric complexed material
Sen'i Gakkaishi,68(2):42-47 2012(Mar.)
Author:Masahiro Mizuno, Yousuke Kamiya, Tomoka Katsuta, Naohisa Oshima, Kouichi Nozaki, Yoshihiko Amano


Design of cellulose dissolving ionic liquids inspired by nature
ChemSusChem,5(2):388-391 2012
Author:Kazutaka Ohira, Yoshikazu Abe, Motoi Kawatsura, Kazuhiko Suzuki, Masahiro Mizuno, Yoshihiko Amano, Toshiyuki Itoh


Comparative study on the ability to produce gentiobiose in cellulose-producing bacteria Asaia bogorensis and Gluconacetobacter xylinus
Journal of Applied Glycoscience,58(4):147-150 2011
Author:Akio Kumagai, Masahiro Mizuno, Kouichi Nozaki, Inder M. Saxena, Yoshihiko Amano


Regulation of adsorption behavior of carbohydrate-binding module family 1 and endo-β-1,4-glucanase onto crystalline cellulose
Journal of Applied Glycoscience,58(4):133-138 2011
Author:Kouichi Nozaki, Hiroto Nishijima, Tsutomu Arai, Masahiro Mizuno, Nobuaki Sato, Yoshihiko Amano


Ultrafine cellulose fibers produced by Asaia bogorensis, an acetic acid bacterium.
Biomacromolecules,12(7):2815-2821 2011
Author:Akio Kumagai, Masahiro Mizuno, Naoto Kato, Kouichi Nozaki, Eiji Togawa, Shigeru Yamanaka, Kazuo Okuda, Inder M. Saxena, Yoshihiko Amano


Hyper-production and characterization of the iota-carrageenase useful for iota-carrageenan oligosaccharide production from a deep-sea bacterium, Microbulbifer thermotolerans JAMB-A94(T), and insight into the unusual catalytic mechanism.
Marine biotechnology,13(3):411-422 2011
Author:Yuji Hatada, Masahiro Mizuno, Zhijun Li, Yukari Ohta


Improvement in the productivity of xylooligosaccharides from waste medium after mushroom cultivation by hydrothermal treatment with suitable pretreatment.
Bioresource Technology,101(15):6006-6011 2010
Author:Sato, N., Shinji, K., Mizuno, M., Nozaki, K., Suzuki, M., Makishima, S., Shiroishi, M., Onoda, T., Takahashi, F., Kanda, K., Amano, Y.


Enzymatic production of glucosylxylose using a cellobiose phosphorylase-yeast combined system
Journal of Applied Glycoscience,58(1):31-34 2010
Author:Akio Kumagai, Sawaki Tada, Kouichi Nozaki, Masahiro Mizuno, Takahisa Kanda, Satoshi Suzuki, Kenichi Kusumoto, Takashi Sasaki, Yutaka Kashiwagi, Yoshihiko Amano


Development of continuous flow type hydrothermal reactor for hemicellulose fraction recovery from corncob.
Bioresource Technology,100:2842-2848 2009
Author:Makishima, S., Mizuno, M., Sato, N., Shinji, K., Suzuki, M., Nozaki, K., Takahashi, F., Kanda, T., Amano, Y.


Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49.
Journal of Molecular Biology,376:210-220 2008
Author:Mizuno, M., Koide, A., Yamamura, A., Akeboshi, H., Yoshida, H., Kamitori, S., Sakano, Y., Nishikawa, A., Tonozuka, T.


Screening and investigation of dye decolorization activities of basidiomycetes
J. Biosci. Bioeng.,105(1):69-72 2008
Author:Nozaki, K., Hai Beh, C., Masahiro Mizuno, M., Isobe, T., Shiroishi, M., Kanda, T., Amano, Y.


Structure and characteristics of an endo-β-1,4-glucanase, isolated from Trametes hirsuta, with high degradation to crystalline cellulose.
Biosci Biotechnol. Biochem.,71:2375-2382 2007
Author:Nozaki, K., Seki, T., Matsui, K., Mizuno, M., Kanda, T., Amano, Y.


Structural analysis of the water-soluble carbohydrate from Asaia bogorensis by NMR spectroscopy
J. Appl. Glycosci.,54:231-233 2007
Author:Kato, N., Mizuno, M., Nakai, Y., Nozaki, K., Suga, H., Kanda, T., Yamanaka, S., Amano, Y.


Viability and cellulose synthesizing ability of Gluconacetobacter xylinus cells under high-hydrostatic pressure.
Extremophiles,11:693-698 2007
Author:Kato, N., Sato, T., Kato, C., Yajima, M., Sugiyama, J., Kanda, T., Mizuno, M., Nozaki, K., Yamanaka, S., Amano, Y.


Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo/maltodextrin-binding protein.
FEBS J.,274:2109-2120 2007
Author:Tonozuka, T., Sogawa, A., Yamada, M., Matsumoto, N., Yoshida, H., Kamitori, S., Ichikawa, K., Mizuno, M., Nishikawa, A., Sakano, Y.


Recovery of soluble sugars from waste medium for Enokitake (Flammulina velutipes) mushroom cultivation with hydrothermal reaction and enzyme digestion
J. Appl. Glycosci.,53:261-266 2006
Author:Makishima, S., Nozaki, K., Mizuno, M., Netsu, E., Shinji, K., Shibayama, T., Kanda, T., Amano, Y.


Structure of a complex of Thermoactinomyces vulgaris R-47 α-amylase 2 with maltohexaose demonstrates the important role of aromatic residues at the reducing end of the substrate binding cleft.
Carbohydrate Research,341:1041-1046 2006
Author:Ohtaki, A., Mizuno, M., Yoshida, H., Tonozuka, T., Sakano, Y., Kamitori, S.


Three-dimensional structure of glucodextranase, a glycoside hydrolase family 15 enzyme.
Biologia Bratislava,60:171-176 2005
Author:Mizuno, M., Tonozuka, T., Ichikawa, T., Kamitori, S., Nishikawa, A., Sakano, Y.


X-ray crystallographic study of glucodextranase from a Gram-positive bacterium, Arthrobacter globiformis I42.
J. Appl. Glycosci.,52:145-151 2005
Author:Mizuno, M., Tonozuka, T., Ichikawa, K., Kamitori, S., Nishikawa, A., Sakano, Y.


Bacterial and archaeal enzymes homologous to glucoamylase: characterization and subsite affinities of a glucoamylase from Thermoactinomyces vulgaris R-47.
Biologia Bratislava,60:161-165 2005
Author:Ichikawa, K., Tonozuka, T., Uotsu-Tomita, R., Mizuno, M., Nishikawa, A., Sakano, Y.


Kinetic studies of site-directed mutational isomalto-dextranase-catalyzed hydrolytic reactions on a 27 MHz quartz-crystal microbalance.
Biochemistry,44:9456-9461 2005
Author:Nihira, T., Mizuno, M., Tonozuka, T., Sakano, Y., Mori, T., Okahata, Y.


Site-directed mutagenesis of Tryptophan 622 of Thermoactinomyces vulgaris R-47 glucoamylase: pH optima and activities of five mutants.
J. Appl. Glycosci.,52:277-279 2005
Author:Ichikawa, K., Tonozuka, T., Mizuno, M., Nishikawa, A., Sakano, Y.


Mutagenesis and structural analysis of Thermoactinomyces vulgaris R-47 α-amylase II (TVA II).
J. Appl. Glycosci.,52:225-231 2005
Author:Mizuno, M., Ichikawa, K., Tonozuka, T., Ohtaki, A., Shimura, Y., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 maltooligosaccharide-metabolizing enzyme homologous to glucoamylase.
Acta Crystallographica Sec. F,61:302-304 2005
Author:Ichikawa, K., Tonozuka, T., Mizuno, M., Tanabe, Y., Kamitori, S., Nishikawa, A., Sakano, Y.


Complex structures of Thermoactinomyces vulgaris R-47 α-amylase 2 with acarbose and cyclodextrins demonstrate the multiple substrate recognition.
J. Biol. Chem.,279:31033-31040 2004
Author:Ohtaki, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.


The crystal structure of Thermoactinomyces vulgaris R-47 α-amylase II (TVA II) complexed with transglycosylated product.
Eur. J. Biochem.,271:2530-2538 2004
Author:Mizuno, M., Tonozuka, T., Uechi, A., Ohtaki, A., Ichikawa, K., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray analysis of Escherichia coli K12 Ygjk protein, a member of glycosyl hydrolase family 63.
Acta Crystallographica Sec. D,60:1284-1285 2004
Author:Tonozuka, T., Uechi, A., Mizuno, M., Ichikawa, K., Nishikawa, A., Sakano, Y.


Structural insights into substrate specificity and function of glucodextranase.
J. Biol. Chem.,279:10575-10583 2004
Author:Mizuno, M., Tonozuka, T., Suzuki, S., Uotsu-Tomita, R., Kamitori, S., Nishikawa, A., Sakano, Y.


Crystallization and preliminary X-ray study of isomaltodextranase from Arthrobacter globiformis.
Acta Crystallographica Sec. D,60:572-573 2004
Author:Akita, M., Mizuno, M., Tonozuka, T., Sakano, Y., Matsui, H., Hidaka, Y., Hatada, Y., Ito, S., Horikoshi, K.


Heterologous production and characterization of Arthrobacter globiformis T6 Isomalto-dextranase.
J. Appl. Glycosci.,51:27-32 2004
Author:Tonozuka, T., Suzuki, S., Ikehara, Y., Mizuno, M., Kim, YK., Nishikawa, A., Sakano, Y.


Mutual conversion of substrate specificities of Thermoactinomyces vulgaris R-47 α-amylases TVA I and TVA II by site-directed mutagenesis.
Carbohydrate Research,388:1553-1558 2003
Author:Ohtaki, A., Iguchi, A., Mizuno, M., Tonozuka, T., Sakano, Y., Kamitori, S.


Crystal structures and substrate specificities of two α-amylases hydrolyzing cyclodextrins and pullulan from Thermoactinomyces vulgaris R-47
Biologia Bratislava,57:71-76 2002
Author:Tonozuka, T., Yokota, T., Ichikawa, K., Mizuno, M., Kondo, S., Nishikawa, A., Kamitori, S., Sakano, Y.


Insights into the electrocatalytic oxidation of cellulose in solution toward applications in direct cellulose fuel cells
,125(27):14576-14582
Author:Yosuke Kageshima, Yurina Ojima, Hiromasa Wada, Sangho Koh, Masahiro Mizuno, Katsuya Teshima, Hiromasa Nishikiori

研究費
受託研究
2015 - 2019 , 麹製造適性に基づく酒造好適米の新たな選抜技術の確立と品種育成
2009 - 2009 , 耐塩性セルラーゼ製剤の耐熱化と利用技術の開発
2008 - 2008 , 耐塩性・耐熱性を有する新規セルラーゼ製剤の開発

科学研究費補助金(研究代表者)
2017 - 2019 , 水熱処理及びイオン液体処理から得られる天然型キシランの構造と酵素による分解挙動 , 基盤研究(C)
2013 - 2014 , モノコンポーネントセルラーゼによるイオン液体処理セルロースの酵素分解性評価 , 若手研究(B)
2010 - 2011 , 海洋性糸状菌が生産するセルラーゼの耐塩・耐熱性機構の解明 , 若手研究(B)

科学研究費補助金(研究分担者)
2015 - 2016 , セルロース高次階層構造に対するセルロース膨潤タンパク質の作用 , 挑戦的萌芽研究
2012 - 2014 , 微生物ナノファイバー紡糸装置における繊維排出機構の解明 , 基盤研究(C)