下坂 誠 (シモサカ マコト)
繊維学部
名誉教授
研究者情報
研究キーワード
- 微生物, 酵素, 遺伝子, 代謝
電話番号
- 0268-21-5341
メールアドレス
- mashimo★shinshu-u.ac.jp
経歴
研究活動情報
論文
- Identification and Characterization of Apigenin 6-C-Glucosyltransferase Involved in Biosynthesis of Isosaponarin in Wasabi (Eutrema japonicum)
Kyoko Mashima, Mayu Hatano, Hideyuki Suzuki, Makoto Shimosaka, Goro Taguchi
Plant Cell Physiol., 60(12), 2733-2743, 2019年12月 - Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum(common buckwheat)
Koja E, Ohata S, Maruyama Y, Suzuki H, Shimosaka M, Taguchi G.
Biosci Biotechnol Biochem, 82(10), 1790-1802, 2018年, 査読有り
- C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants
Takamitsu Ito, Shunsuke Fujimoto, Fumiaki Suito, Makoto Shimosaka, Goro Taguchi
The Plant Journal, 91, 187-198, 2017年 - pFungiway: a series of plasmid vectors used for gene manipulation in fungi.
Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Toru Okuhara, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka
Ann. Microbiol., 66(2), 825–832, 2016年 - Taste characterization of the fruit body derived from a novel strain belonging to the genus Pleurotus.
Yoshihito Azumi, Kahori Doi, Kana Kogiso, Goro Taguchi, Makoto Shimosaka, Kenji Ouchi, Satoshi Inatomi
Mushroom Sci Biotechnol, 24(3), 129-135, 2016年 - ヒラタケ属新品種子実体がすまし汁の呈味性に及ぼす影響
安積良仁、小木曽加奈、土居香織、下坂 誠、大内謙二、稲冨 聡
日本きのこ学会誌, 24(1), 29-35, 2016年 - Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis.
Miruku Ueda, Makoto Shimosaka and Ryoichi Arai
Acta Cryst, F71, 1516-1520, 2015年 - Crystal structure of ChiL, a chitinase from Chitiniphilus shinanonensis
Miruku Ueda, Makoto Shimosaka, Ryoichi Arai
Photon Factory Activity Report 2014, 32(B), 206, 2015年 - pFungiway: a series of plasmid vectors used for gene manipulation in fungi
Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka
Annals of Microbiology, 2015年, 査読有り - Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase.
Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.
Plant Biotech., 31, 519-524, 2014年 - Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.
Plant J., 80(3), 437–448, 2014年
- Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes
Shuichi Sekiya, Masato Yamada, Kou Shibata, Toru Okuhara, Masumi Yoshida, Satoshi Inatomi, Goro Taguchi, and Makoto Shimosaka
J. Biosci. Bioeng., 115(4), 360-365, 2013年 - キチン分解細菌Chitiniphilus shinanonensis由来の新規キチン分解酵素を用いたN-アセチルグルコサミンの生産
下坂 誠
バイオインダストリー, 30(4), 52-59, 2013年 - Knockdown of electron transfer flavoprotein b-subunit reduced TGF-b-induced a-SMA mRNA expression but not COL1A1 in fibroblast-populated three-dimensional collagen gel cultures
Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka
JOURNAL OF DERMATOLOGICAL SCIENCE, 68, 179-186, 2012年 - Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold, Aspergillus oryzae
Akihiro Sugita, Ayako Sugii, Kazuaki Sato, Xiao-Yong Zhang, An-Lan Dai, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 76(1), 193-195, 2012年 - Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase
Lanxiang Huang, Ewelina Garbulewska, Kazuaki Sato,Yuichi Kato, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
J. Biosci. Bioeng., 113(3), 293-299, 2012年 - Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium, Chitiniphilus shinanonensis
Lanxiang Huang, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 76(3), 517-522, 2012年 - バイオマスに由来する新規ナノ・ハイブリッドファイバーに対する微生物分解過程の解明
下坂 誠
IFO Research Communications, 25, 129-144, 2011年 - Chitin nanowhiskers mediate transformation of Escherichia coli by exogenous plasmid DNA
Alisa Mera, Jun Araki, Takashi Ohtsuki, Makoto Shimosaka, and Naoto Yoshida
J. Biotechnol. Biomater., 1(6), 114, 2011年 - Identification of ETFB as a candidate protein that participates in the mechanoregulation of fibroblast cell number in collagen gel culture.
Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka
JOURNAL OF DERMATOLOGICAL SCIENCE, 64, 119–126, 2011年 - Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of cultivated mushroom Flammulina velutipes
Tomomitsu Okamoto, Masato Yamada, Shuichi Sekiya, Toru Okuhara, Goro Taguchi, Satoshi Inatomi and Makoto Shumosaka
Biosci. Biotechnol. Biochem., 74(11), 2327-2329, 2010年 - Construction and analysis of a bacterial community exhibiting a strong chitinolytic activity
Kazuaki Sato, Yuichi Kato, Ayabi Fukamachi, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 74(3), 636-640, 2010年 - Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Kazuaki Sato, Yasuhito Azama, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka
Journal of Bioscience and Bioengineering, 109(5), 472-478, 2010年 - Agrobacterium tumefaciens-mediated transformation of antifungal lipopeptide producing fungus Coleophoma empetri F-11899
Masato Yamada, Kazunobu Yawata, Yohsuke Orino, Satoshi Ueda, Yasuhiro Isogai, Goro Taguchi, Makoto Shimosaka, and Seiji Hashimoto
Current Genetics, 55, 623-630, 2009年 - Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
Kazuaki Sato, Yuichi Kato, Goro Taguchi, Masahiro Nogawa, Akira Yokota, and Makoto Shimosaka;
J. Gen. Appl. Microbiol., 55(2), 147-153, 2009年 - Gene cloning, expression, and characterization ofa second b-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9
Xiqian Lan, Xin Zhang, Ritsuko Kodaira, Zeyang Zhou, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(2), 492-498, 2008年 - Characterization of a gene conferring red fluorescence isolated from an environmental DNA library constructed from soilbacteria
Xiqian Lan, Kazuaki Sato, Goro Taguchi, Zeyang Zhou, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(7), 1908-1914, 2008年 - Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression inPichia pastoris
Hiroshi Toda, Naoki Nagahata, Yoshihiko Amano, Kouichi Nozaki, Takahisa Kanda, Mitsuo Okazaki, and Makoto Shimosaka
Biosci. Biotechnol. Biochem., 72(12), 3142-3147, 2008年 - Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in thebasidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
Masato Yamada, Michihisa Kurano, Satoshi Inatomi, Goro Taguchi, Mitsuo Okazaki, and Makoto Shimosaka
FEMS Microbiol. Lett., 289(2), 130-137, 2008年 - Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
Masato Yamada, Sou Sakuraba, Kou Shibata, Goro Taguchi, Satoshi Inatomi, Mitsuo Okazaki, and Makoto Shimosaka
FEMS Microbiol. Lett., 254(1), 165-172, 2006年 - Analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis
Makoto Shimosaka, Kazuaki Sato, Naohide Nishiwaki, Takashi Miyazawa, and Mitsuo Okazaki
J. Biosci. Bioeng., 100(5), 545-550, 2005年
書籍等出版物
講演・口頭発表等
- Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis
Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – Development of gene disruption by an allelic exchange system –
Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – chiG, chiJ,and chiK coding for unique chitin-degrading enzymes –
Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka
10th International Conference of the Asian Pacific Chitin Chitosan Symposium, 2013年 - Isolation of genes coding for chitin-degrading enzymes in the chitinolytic bacterium, Chitiniphilus shinanonensis
Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA
6th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda., 2011年 - Analysis of genes coding for chitin-degrading enzymes from the bacterium Chitiniphilus shinanonensis
Lanxiang HUANG, Masahiro NOGAWA, Goro TAGUCHI, and Makoto SHIMOSAKA
International Conference of Future Textile, 2010年 - Agrobacterium tumefaciens-mediated transformation as a toolfor genomics in an industrially important fungus.
Masato Yamada, Kazuki Yawata1,Seiji Hashimoto and Makoto Shimosaka
The Society for Industrial Microbiology, The Annual Meeting and Exhibition 2009, 2009年06月 - Isolation and Characterization of a Novel Chitinolytic Bacterium, -Construction of Stable Chitinolytic Microbial Community-.
Sato K, Aoyama H, Kato Y, Nogawa M, Taguchi G, Yokota A, and Shimosaka M
7th East Asian Symposium on Polymers for Advanced Technology, 2009年 - Degradation of chitin by a novel bacterium Chitiniphilus shinanonensis originated from a bacterial community expressing a chitinolytic activity
Sato K, Shizume A, Aoyama H, Kato Y, Nogawa M, Taguchi G, and Shimosaka M
International Conference on Advanced Fiber/Textile Materials, 2009年 - Analysis of Bacterial Consortium Growing on Chitin Flake by Using Metagenomic Approach
Sato K., Azama Y., Kodaira R., Taguchi G., Shimosaka M
2007年, The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda - Isolation, gene cloning, and characterization of the chitinolytic enzymes in the bacterium Aeromonas hydrophila strain SUWA-9
Lan, X., Zhang, X., Hu, J., and Shimosaka, M.
2007年, The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda - Functional analysis of a novel C-terminal domain found in a chitosanase from the koji mold Aspergillus oryzae
Makoto Shimosaka, Ayako Sugii, Akihiro Sugita, Xiao-Yong Zhang, and Kazuaki Sato
2006年08月, 第10回キチン・キトサン国際会議
