信州大学HOMEENGLISH交通・キャンパス案内

研究者総覧研究者総覧

研究者、研究内容などで検索
項目別検索はこちら

下坂 誠  シモサカ マコト

教員組織学術研究院(繊維学系)電話番号0268-21-5341
教育組織繊維学部 応用生物科学科FAX番号0268-21-5341
職名教授メールアドレスmashimo@shinshu-u.ac.jp
住所〒386-8567 長野県上田市常田3-15-1ホームページURLhttp://fiber.shinshu-u.ac.jp/biotech/home.html

更新日:2019/08/12

プロフィール

研究分野
応用微生物学
現在の研究課題
未利用微生物資源の探索

微生物の生産するキチン・キトサン分解酵素に関する研究

糸状菌の遺伝子操作系の開発と有用菌株育種への応用

所属学会
所属学会
糸状菌分子生物学研究会
日本農芸化学会
日本生物工学会
日本キチン・キトサン学会
日本きのこ学会
学歴
出身大学院
1984 , 京都大学 , 農学研究科

出身学校・専攻等(大学院を除く)
1979 , 京都大学 , 農学部

取得学位
農学博士 , 京都大学
研究職歴等
研究職歴
2004- , 信州大学繊維学部 教授
1998-2004 , 信州大学繊維学部 助教授
1996-1998 , 信州大学遺伝子実験施設 助教授
1993-1996 , 信州大学繊維学部 助教授
1987-1993 , 信州大学繊維学部 講師
1986-1987 , 信州大学繊維学部 助手

留学歴
1989-1989 , 米国コーネル大学研究員
1988-1988 , 米国ノートルダム大学研究員

研究活動業績

研究業績(著書・
発表論文等)
著書
応用生物学入門 第8章 微生物の有用物質
オーム社 2010
Author:平林公男・白井孝治共編


ポイントがわかる分子生物学(第2版)3-10 ブロッティング(サザン、ノーザン、ウエスタン)
丸善 2010
Author:真野佳博・川向 誠編著


キチン・キトサンの開発技術(平野茂博監修) 第7章「キトサナーゼ遺伝子のクローニングと解析」
シーエムシー出版 2009


論文
Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum(common buckwheat)
Biosci Biotechnol Biochem 2018
Author:Koja E, Ohata S, Maruyama Y, Suzuki H, Shimosaka M, Taguchi G.


C-Glycosyltransferases catalyzing the formation of di-C-glucosyl flavonoids in citrus plants
The Plant Journal,91:187-198 2017
Author:Takamitsu Ito, Shunsuke Fujimoto, Fumiaki Suito, Makoto Shimosaka, Goro Taguchi


Taste characterization of the fruit body derived from a novel strain belonging to the genus Pleurotus.
Mushroom Sci Biotechnol,24(3):129-135 2016
Author:Yoshihito Azumi, Kahori Doi, Kana Kogiso, Goro Taguchi, Makoto Shimosaka, Kenji Ouchi, Satoshi Inatomi


pFungiway: a series of plasmid vectors used for gene manipulation in fungi.
Ann. Microbiol. ,66(2):825–832 2016
Author:Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Toru Okuhara, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka


ヒラタケ属新品種子実体がすまし汁の呈味性に及ぼす影響
日本きのこ学会誌,24(1):29-35 2016
Author:安積良仁、小木曽加奈、土居香織、下坂 誠、大内謙二、稲冨 聡


Crystal structure of ChiL, a chitinase from Chitiniphilus shinanonensis
Photon Factory Activity Report 2014,32(B):206 2015
Author:Miruku Ueda, Makoto Shimosaka, Ryoichi Arai


Expression, purification, crystallization and X-ray diffraction analysis of ChiL, a chitinase from Chitiniphilus shinanonensis.
Acta Cryst,F71:1516-1520 2015
Author:Miruku Ueda, Makoto Shimosaka and Ryoichi Arai


pFungiway: a series of plasmid vectors used for gene manipulation in fungi
Annals of Microbiology 2015
Author:Ryohei Nishikawa, Masumi Yoshida, Tomotsugu Noda, Goro Taguchi, Satoshi Inatomi, Makoto Shimosaka


Production of C-glucosides of flavonoids and related compounds by Escherichia coli expressing buckwheat C-glucosyltransferase.
Plant Biotech.,31:519-524 2014
Author:Ito, T., Fujimoto, S., Shimosaka, M., Taguchi, G.


Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon.
Plant J.,80(3):437–448 2014
Author:Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M., Taguchi, G.


キチン分解細菌Chitiniphilus shinanonensis由来の新規キチン分解酵素を用いたN-アセチルグルコサミンの生産
バイオインダストリー,30(4):52-59 2013
Author:下坂 誠


Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the basidiomycete Flammulina velutipes
J. Biosci. Bioeng.,115(4):360-365 2013
Author:Shuichi Sekiya, Masato Yamada, Kou Shibata, Toru Okuhara, Masumi Yoshida, Satoshi Inatomi, Goro Taguchi, and Makoto Shimosaka


Knockdown of electron transfer flavoprotein b-subunit reduced TGF-b-induced a-SMA mRNA expression but not COL1A1 in fibroblast-populated three-dimensional collagen gel cultures
Journal of Dermatological Science,68:179-186 2012
Author:Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka


Heterologous expression and functional characterization of a novel chitinase from the chitinolytic bacterium, Chitiniphilus shinanonensis
Biosci. Biotechnol. Biochem.,76(3):517-522 2012
Author:Lanxiang Huang, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase
J. Biosci. Bioeng.,113(3):293-299 2012
Author:Lanxiang Huang, Ewelina Garbulewska, Kazuaki Sato,Yuichi Kato, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Cloning and characterization of a gene coding for a major extracellular chitosanase from the koji mold, Aspergillus oryzae
Biosci. Biotechnol. Biochem.,76(1):193-195 2012
Author:Akihiro Sugita, Ayako Sugii, Kazuaki Sato, Xiao-Yong Zhang, An-Lan Dai, Goro Taguchi, and Makoto Shimosaka


バイオマスに由来する新規ナノ・ハイブリッドファイバーに対する微生物分解過程の解明
IFO Research Communications,25:129-144 2011
Author:下坂 誠


Chitin nanowhiskers mediate transformation of Escherichia coli by exogenous plasmid DNA
J. Biotechnol. Biomater.,1(6):114 2011
Author:Alisa Mera, Jun Araki, Takashi Ohtsuki, Makoto Shimosaka, and Naoto Yoshida


Identification of ETFB as a candidate protein that participates in the mechanoregulation of fibroblast cell number in collagen gel culture.
Journal of Dermatological Science,64:119–126 2011
Author:Shigenari Hirokawa, Tomomasa Shimanuki, Hiroyuki Kitajima, Yasutomo Nishimori, and Makoto Shimosaka


Agrobacterium tumefaciens-mediated transformation of the vegetative dikaryotic mycelium of cultivated mushroom Flammulina velutipes
Biosci. Biotechnol. Biochem.,74(11):2327-2329 2010
Author:Tomomitsu Okamoto, Masato Yamada, Shuichi Sekiya, Toru Okuhara, Goro Taguchi, Satoshi Inatomi and Makoto Shumosaka


Construction and analysis of a bacterial community exhibiting a strong chitinolytic activity
Biosci. Biotechnol. Biochem.,74(3):636-640 2010
Author:Kazuaki Sato, Yuichi Kato, Ayabi Fukamachi, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Journal of Bioscience and Bioengineering,109(5):472-478 2010
Author:Kazuaki Sato, Yasuhito Azama, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria
J. Gen. Appl. Microbiol.,55(2):147-153 2009
Author:Kazuaki Sato, Yuichi Kato, Goro Taguchi, Masahiro Nogawa, Akira Yokota, and Makoto Shimosaka;


Agrobacterium tumefaciens-mediated transformation of antifungal lipopeptide producing fungus Coleophoma empetri F-11899
Current Genetics,55:623-630 2009
Author:Masato Yamada, Kazunobu Yawata, Yohsuke Orino, Satoshi Ueda, Yasuhiro Isogai, Goro Taguchi, Makoto Shimosaka, and Seiji Hashimoto


Gene cloning of cellobiohydrolase II from the white rot fungus Irpex lacteus MC-2 and its expression inPichia pastoris
Biosci. Biotechnol. Biochem.,72(12):3142-3147 2008
Author:Hiroshi Toda, Naoki Nagahata, Yoshihiko Amano, Kouichi Nozaki, Takahisa Kanda, Mitsuo Okazaki, and Makoto Shimosaka


Characterization of a gene conferring red fluorescence isolated from an environmental DNA library constructed from soilbacteria
Biosci. Biotechnol. Biochem.,72(7):1908-1914 2008
Author:Xiqian Lan, Kazuaki Sato, Goro Taguchi, Zeyang Zhou, and Makoto Shimosaka


Gene cloning, expression, and characterization ofa second b-N-acetylglucosaminidase from the chitinolytic bacterium Aeromonas hydrophila strain SUWA-9
Biosci. Biotechnol. Biochem.,72(2):492-498 2008
Author:Xiqian Lan, Xin Zhang, Ritsuko Kodaira, Zeyang Zhou, and Makoto Shimosaka


Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in thebasidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
FEMS Microbiol. Lett.,289(2):130-137 2008
Author:Masato Yamada, Michihisa Kurano, Satoshi Inatomi, Goro Taguchi, Mitsuo Okazaki, and Makoto Shimosaka


Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
FEMS Microbiol. Lett.,254(1):165-172 2006
Author:Masato Yamada, Sou Sakuraba, Kou Shibata, Goro Taguchi, Satoshi Inatomi, Mitsuo Okazaki, and Makoto Shimosaka


Analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis
J. Biosci. Bioeng.,100(5):545-550 2005
Author:Makoto Shimosaka, Kazuaki Sato, Naohide Nishiwaki, Takashi Miyazawa, and Mitsuo Okazaki


学会発表
Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – chiG, chiJ,and chiK coding for unique chitin-degrading enzymes –
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :PE-02 2013
Author:Moe Nakano, Jiayi Yu, Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka


Analysis of genes coding for chitinolytic enzymes in the bacterium, Chitiniphilus shinanonensis – Development of gene disruption by an allelic exchange system –
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :PE-01 2013
Author:Norie Sonoda, Jiayi Yu, Arisa Shizume, Masahiro Nogawa, Goro Taguchi, Makoto Shimosaka


Isolation and characterization of genes encoding chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis
10th International Conference of the Asian Pacific Chitin Chitosan Symposium , :IE-06 2013
Author:Makoto Shimosaka, Lanxiang Huang, Norie Sonoda, Moe Nakano, Masahiro Nogawa, Goro Taguchi


Isolation of genes coding for chitin-degrading enzymes in the chitinolytic bacterium, Chitiniphilus shinanonensis
6th International Conference on Adovanced Fiber/Textile Materials 2009 in Ueda. , :IP-08 2011
Author:Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Analysis of genes coding for chitin-degrading enzymes from the bacterium Chitiniphilus shinanonensis
International Conference of Future Textile 2010
Author:Lanxiang Huang, Masahiro Nogawa, Goro Taguchi, and Makoto Shimosaka


Agrobacterium tumefaciens-mediated transformation as a toolfor genomics in an industrially important fungus.
The Society for Industrial Microbiology, The Annual Meeting and Exhibition 2009 , :131 2009(Jun.)
Author:Masato Yamada, Kazuki Yawata1,Seiji Hashimoto and Makoto Shimosaka


Degradation of chitin by a novel bacterium
 Chitiniphilus shinanonensis originated from a bacterial community expressing a chitinolytic activity
International Conference on Advanced Fiber/Textile Materials 2009
Author:Sato K, Shizume A, Aoyama H, Kato Y, Nogawa M, Taguchi G, and Shimosaka M


Isolation and Characterization of a Novel Chitinolytic Bacterium, 
-Construction of Stable Chitinolytic Microbial Community-.
7th East Asian Symposium on Polymers for Advanced Technology 2009
Author:Sato K, Aoyama H, Kato Y, Nogawa M, Taguchi G, Yokota A, and Shimosaka M


Isolation, gene cloning, and characterization of the chitinolytic enzymes in the bacterium Aeromonas hydrophila strain SUWA-9
The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda 2007
Author:Lan, X., Zhang, X., Hu, J., and Shimosaka, M.


Analysis of Bacterial Consortium Growing on Chitin Flake by Using Metagenomic Approach
The 4th International Conference on Advanced Fiber/Textile Materials 2007 in Ueda 2007
Author:Sato K., Azama Y., Kodaira R., Taguchi G., Shimosaka M


Functional analysis of a novel C-terminal domain found in a chitosanase from the koji mold Aspergillus oryzae
第10回キチン・キトサン国際会議 2006(Aug.)
Author:Makoto Shimosaka, Ayako Sugii, Akihiro Sugita, Xiao-Yong Zhang, and Kazuaki Sato

共同研究等希望テーマ
キチン・キトサン分解酵素を用いたバイオマスの有効利用
未利用微生物資源の探索
ヒカリゴケの人工培養
提供可能な資源
フザリウム菌保存菌株

教育活動実績

授業等
2019 , 前期 , 食品工学
2019 , 前期 , 遺伝子工学
2019 , 後期 , 応用微生物学