論文 Phenomenological interpretations of the mechanism for the concentration-dependent positive effect of antibiotic lincomycin on Streptomyces coelicolor A3(2) Applied and Environmental Microbiology,89(10) 2023 Author:Mukai K, Shibayama T, Imai Y, Hosaka T
Heterologous biosynthesis of myxobacterial lanthipeptides melittapeptins Applied Microbiology and Biotechnology, in press 2023 Author:Kaweewan I, Mukai K, Rukthanapitak P, Nakagawa H, Hosaka T, Kodani S
Heterologous production and structure determination of a new lanthipeptide sinosporapeptin using a cryptic gene cluster in an actinobacterium Sinosporangium siamense Journal of Microbiology,61:641-648 2023 Author:Saito K, Mukai K, Kaweewan I, Nakagawa H, Hosaka T, Kodani S
Unique physiological and genetic features of ofloxacin-resistant Streptomyces mutants Applied and Environmental Microbiology,88(3) 2022 Author:Hoshino K, Hamauzu R, Nakagawa H, Kodani S, Hosaka T
A putative mechanism underlying secondary metabolite overproduction by Streptomyces strains with a 23S rRNA mutation conferring erythromycin resistance Applied Microbiology and Biotechnology,104(5):2193-2203 2020 Author:Hoshino K, Imai Y, Mukai K, Hamauzu R, Ochi K, Hosaka T
Lincomycin-induced secondary metabolism in Streptomyces lividans 66 with a mutation in the gene encoding the RNA polymerase beta subunit Current Microbiology,77:2933-2939 2020 Author:Mukai K, Kobayashi M, Hoshino K, Maruyama T, Hayashi D, Hamauzu R, Hosaka T
Isolation and structure determination of a new cytotoxic peptide curacozole from Streptomyces curacoi based on genome mining The Journal of Antibiotics,72:1-7 2019 Author:Kodani S, Kaweewan I, Komaki H, Hemmi H, Hoshino K, Hosaka T, Isokawa G, and Oyoshi T
A possible mechanism for lincomycin induction of secondary metabolism in Streptomyces coelicolor A3(2) Antonie van Leeuwenhoek,111:705-716 2018 Author:Ishizuka M, Imai Y, Mukai K, Shimono K, Hamauzu R, Ochi K, Hosaka T
Combined Drug-Resistance Mutations Substantially Enhance Enzyme Production in Paenibacillus agaridevorans Journal of Bacteriology,200:e00188-18 2018 Author:Funane K, Tanaka Y, Hosaka T, Murakami K, Miyazaki T, Shiwa Y, Gibu S, Inaoka T, Kasahara K, Fujita N, Yoshikawa H, Hiraga Y, Ochi K
Lincomycin at subinhibitory concentrations potentiates secondary metabolite production by Streptomyces spp. Applied and Environmental Microbiology,81:3869-3879 2015 Author:Imai Y, Sato S, Tanaka Y, Ochi K, Hosaka T.
New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters Applied Microbiology and Biotechnology,97:87-98 2013 Author:Ochi K. and Hosaka T.
Heterologous expression of plant RelA/SpoT homolog results in increased stresstolerance inSaccharomyces cerevisiae by accumulating the bacterial alarmone ppGpp Microbiology,158:2213 - 2224 2012 Author:Ochi K., Nishizawa T., Inaoka T., Yamada Y., Hashimoto K., Hosaka T., Okamoto S., Ozeki, Y.
Development of the ability to produce secondary metabolites in Streptomyces through the acquisition of erythromycin resistance The journal of Antibiotics,65:323–326 2012 Author:Imai Y., Fujiwara T., Ochi K., and Hosaka, T.
Rare earth elements activate the secondary metabolite-biosynthesis gene clusters in Streptomyces coelicolor A3(2) J Antibiot,63:477-481 2010 Author:Tanaka, Y., Hosaka T., and Ochi, K.
Antibacterial discovery in actinomycetes strains with mutations in RNA polymerase or ribosomal protein S12 Nature Biotechnology,27(5):462-464 2009 Author:Hosaka T., Ohnishi-Kameyama M., Muramatsu H., Murakami K., Tsurumi Y., Kodani S., Yoshida M., Fujie A., and Ochi K..
Bacterialalarmone, guanosine 5'-diphosphate 3'-diphosphate (ppGpp), predominantly binds the beta' subunit of plastid-encoded plastid RNA polymerasein chloroplasts. Chembiochem,10:1227-1233 2009 Author:Sato M., Takahashi K, Ochiai Y, Hosaka T, Ochi K, Nabeta K
Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations Appl Environ Microbiol,74:2834-2840 2008 Author:Wang G., Hosaka T., and Ochi K.
Mutations in rsmG, encoding a16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2) J Bacteriol,189:3876-3883 2007 Author:Nishimura, K., Hosaka T., Tokuyama S., Okamoto S., and Ochi K.
Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants J Bacteriol,189:6068-6073 2007 Author:Nishimura K., Johansen SK., Inaoka T., Hosaka T., Tokuyama S., Tahara Y., Okamoto S., Kawamura F., Douthwaite S., and Ochi K. .
Improvement of alpha-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168 Appl Environ Microbiol,72:71-77 2006 Author:Kurosawa, K., T. Hosaka, N. Tamehiro, T. Inaoka and K. Ochi
EshA accentuates ppGpp accumulation andis conditionally required for antibiotic production in Streptomyces coelicolor A3(2) J Bacteriol,188:4952-4961 2006 Author:Saito, N., J. Xu, T. Hosaka, S. Okamoto, H.Aoki, M. J. Bibb and K. Ochi.
Physiological analysis of the stringent response elicited in an extreme thermophilic bacterium, Thermus thermophilus J Bacteriol,188:7111-7122 2006 Author:Kasai, K., T. Nishizawa, K. Takahashi, T. Hosaka, H. Aoki and K. Ochi
Increased expression of ribosome recycling factor is responsible for the enhanced protein synthesis during the late growth phase in an antibiotic-overproducing Streptomyces coelicolor ribosomal rpsL mutant Mol Microbiol,61:883-897 2006 Author:Hosaka, T., J. Xu, and K. Ochi
Effects of Escherichia coli ribosomal protein S12 mutations on cell-free protein synthesis Eur J Biochem,271:1127-1134 2004 Author:Chumpolkulwong, N., C. Hori-Takemoto, T. Hosaka, T. Inaoka, T. Kigawa, M. Shirouzu, K. Ochi and S. Yokoyama
The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli Mol Genet Genomics,271:317-324 2004 Author:Hosaka, T., N. Tamehiro, N. Chumpolkulwong, C. Hori-Takemoto, M. Shirouzu, S. Yokoyama and K.Ochi
Structural basis for transcription regulation by alarmone ppGpp Cell,117:299-310 2004 Author:Artsimovitch, I., V. Patlan, S. Sekine, M. N. Vassylyeva, T. Hosaka, K. Ochi, S. Yokoyama and D. G. Vassylyev
Ribosome engineering and secondary metabolite production Adv Appl Microbiol,56:155-184 2004 Author:Ochi, K., S. Okamoto, Y. Tozawa, T. Inaoka, T. Hosaka, J. Xu and K. Kurosawa
Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2) J Bacteriol,185:601-609 2003 Author:Okamoto, S., A. Lezhava, T. Hosaka, Y. Okamoto-Hosoya and K. Ochi
Innovative approach for improvement of an antibiotic-overproducing industrial strain of Streptomyces albus Appl Environ Microbiol,69:6412-6417 2003 Author:Tamehiro, N., T. Hosaka, J. Xu, H. Hu, N. Otake and K. Ochi
An aberrant protein synthesis activity is linked with antibiotic overproduction in rpsL mutants of Streptomyces coelicolor A3(2) Microbiology,149:3299-309 2003 Author:Okamoto-Hosoya, Y., T. Hosaka and K. Ochi
Acetylacetoin synthase as a marker enzyme for detecting the 2,3-butanediol cycle J Biosci Bioeng,93:248-251 2002 Author:Ui, S., T. Hosaka, K. Mizutani, T. Ohtsuki and A. Mimura
Characterization of the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase gene from Bacillus cereus YUF-4 J Biosci Bioeng,91:539-544 2001 Author:Hosaka, T., S. Ui, T. Ohtsuki, A. Mimura, M. Ohkuma and T. Kudo
Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4 Biosci Biotechnol Biochem,63:199-201 1999 Author:Hosaka, T., S. Ui and A. Mimura
Discovery of a new mechanism of 2,3-butanediol stereoisomer formation Bacillus cereus YUF-4 J Biosci Bioeng,85:79-83 1998 Author:Ui, S., T. Hosaka, K. Watanabe, and A. Mimura
Purification andproperties of acetylacetoin synthase from Bacillus sp. YUF-4 Biosci Biotechnol Biochem,62:795-797 1998 Author:Ui, S., T. Hosaka, K. Mizutani and A. Mimura
講演・口頭発表等 Molecular physiological studies on actinomycetes for a fundamental understanding of antibiotics 第93回日本細菌学会総会シンポジウム 2020(Feb. 19) Presenter:保坂毅（invited）
Analysis of mechanisms for antibiotic induction of secondary metabolism 18th International Symposium on the Biology of Actinomycetes (JEJU/KOREA) 2017 Presenter:SHIMONO K, ISHIZUKA M, IMAI Y, OCHI K, and HOSAKA T
Ribosome-targeting antibiotics at subinhibitory concentrations potentiate secondary metabolite production by streptomycetes Society for Industrial Microbiology and Biotechnology Annual Meeting 2015 Presenter:Hosaka T（Invited）
Activation of the potential to produce secondary metabolites in actinomycetes by modulating the RNA polymerase and/or ribosome ISIM2014 (Dalian/China） 2014 Presenter:Hosaka T (Invited)
Lincomycin at subinhibitory concentrations activates the potential of Streptomyces strains to produce secondary metabolites 17th International Symposium on the Biology of Actinomycetes [ISBA’17]（Aydin/Turkey） 2014 Presenter:Imai Y., Sato S., Tanaka Y., Ochi K., and Hosaka T.
Activation of secondary metabolite production in Streptomyces spp. by exposure to subinhibitory concentrations of lincomycin. IUMS2014 [XIVth International Congress of Bacteriology and Applied Microbiology] 2014 Presenter:Imai Y., Sato S., Tanaka Y., Ochi K., and Hosaka T.
Activation of silent genes in Streptomyces sp. 631689 by generating a spontaneous gentamicin-resistant mutant 15th International Symposium on the Biology of Actinomycetes 2009 Presenter:Hosaka, T., T. Fujiwara, Y. Imai, K. Sen and K. Ochi
The impact of “ribosome engineering” on antibiotic discovery Japan-UK Workshop 2008 (Genomics of Antibiotic-producing Actinomycete: Implicationsand Applications) 2008 Presenter:Hosaka T (invited)
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