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保地 眞一  ホチ シンイチ

教員組織学術研究院(繊維学系)電話番号0268-21-5350
教育組織繊維学部 応用生物科学科FAX番号0268-21-5830
職名教授メールアドレスshochi@shinshu-u.ac.jp
住所〒386-8567 長野県上田市常田3-15-1ホームページURLhttp://fiber.shinshu-u.ac.jp/hochi/

プロフィール

兼担研究科・学部
大学院総合理工学研究科(修士)
大学院総合医理工学研究科(博士)
研究分野
生殖生物学
低温生物学
発生工学
現在の研究課題
哺乳動物資源の保存と有効利用
キーワード:凍結乾燥 形質転換動物 顕微授精 , 1996-
所属学会
所属学会
日本繁殖生物学会
日本畜産学会
日本卵子学会

所属学会役職担当
2019-2020 , 日本繁殖生物学会 , 理事
2018-2018 , 日本繁殖生物学会 , 第111回大会長
2009-2018 , 日本繁殖生物学会 , 評議員/評議委員
2007-2018 , 日本卵子学会 , 評議員/代議員
学歴
出身大学院
1986 , 岡山大学大学院 , 農学研究科 , 畜産学専攻

出身学校・専攻等(大学院を除く)
1984 , 岡山大学 , 農学部 , 畜産学科

取得学位
1991 , 博士(農学) 論農博1695号 , 京都大学
受賞学術賞
2006 , 日本実験動物技術者協会 研究奨励賞
2002 , 日本繁殖生物学会 学術賞
研究職歴等
研究職歴
2008- , 信州大学繊維学部 教授
1999-2008 , 信州大学繊維学部 助教授/准教授
1996-1999 , 信州大学繊維学部 助手
1995-1996 , 帯広畜産大学畜産学部 客員助教授
1992-1995 , 帯広畜産大学畜産学部 寄附講座教員(助手相当)
1986-1992 , 雪印乳業生物科学研究所 研究員

留学歴
1995-1996 , University of Guelph (Canada)

研究活動業績

研究業績(著書・
発表論文等)
著書
【著11】 Embryonic stem cells and gene modification in rat (Chapter 5)
Humana Press , "Rat Genomics (Eds. G.T. Hayman, J.R. Smith, M.R. Dwinell and M. Shimoyama) / Methods in Molecular Biology" , 2018:115-130 2019
Author:M. Hirabayashi, A. Takizawa, S. Hochi


【著10】 Organ generation from knocked-out rat blastocysts complemented with pluripotent stem cells (Chapter 18)
Humana Press , "Microinjection (Eds. C. Liu and Y. Du) / Methods in Molecular Biology" , 1874:313-326 2019
Author:M. Hirabayashi, S. Hochi


【著9】 Intracytoplasmic sperm injection in cattle (Chapter 2)
Nova Science Publishers , "In Vitro Fertilization (Ed. S.P. Steiger)" , :35-63 2011
Author:S. Hochi, H. Abdalla, M. Hirabayashi


【著8】 Rat embryonic stem cells: establishement and their use for transgenesis (Chapter 22)
InTech , "Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Application (Ed. C. Atwood)" , :397-410 2011
Author:M. Hirabayashi, S. Hochi


【著7】 Generation of transgenic rats by ooplasmic injection of sperm cells exposed to exogenous DNA (Chapter 9)
Humana Press , "Rat Genomics (Ed. I. Anegon) / Methods in Molecular Biology" , 597:127-136 2010
Author:M. Hirabayashi, S. Hochi


【著6】 Cloning in the rat (Chapter 10)
Taylar and Francis Books , "Epigenetic Risks of Cloning (Ed. A. Inui)" , :165-175 2006
Author:M. Hirabayashi, S. Hochi


【著5】 Systematic production of transgenic rats by pronuclear DNA microinjection (Section 6)
Hokuto Shobo , "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)" , :243-250 2001
Author:M. Hirabayashi, S. Hochi


【著4】 Cryopreservation of in vitro-produced bovine embryos (Section 7)
Hokuto Shobo , "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)" , :333-339 2001
Author:S. Hochi


【著3】 卵子の応用(臨床)技術;卵子の凍結保存法:未成熟卵・成熟卵(第四章 第三節)
養賢堂出版 , "生殖工学のための講座「卵子研究法」(佐藤 英明・鈴木 秋悦 編)" , :316-322 2001
Author:保地 眞一


【著2】 Cryopreservation of preimplantation embryos and follicular oocytes in cattle and horses (Section 6)
Shoukadoh Booksellers , "Reproductive Biology Update (Eds. H. Miyamoto and N. Manabe)" , :425-438 1998
Author:S. Hochi


【著1】 第III部 発生工学的方法、第10章 トランスジェニック動物、2. ラット
東京化学同人 , 新生化学実験講座 動物実験法 , 19:289-293 1991
Author:結城 惇, 保地 真一


論文
【論119】 Nylon mesh cryodevice for bovine mature oocytes, easily removable excess vitrification solution
Cryobiology,90:96-99 2019
Author:S. Chinen, T. Yamanaka, K. Nakayama, H. Watanabe, Y. Akiyama, M. Hirabayashi, S. Hochi


【論118】 Supplementary cryoprotective effect of carboxylated ε-poly-L-lysine during vitrification of rat pancreatic islets
Cryobiology,88:70-74 2019
Author:K. Nakayama, T. Yamanaka, Y. Tamada, M. Hirabayashi, S. Hochi


【論117】 Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats
Nat. Commun.,10(1):451 2019
Author:T. Goto, H. Hara, M. Sanbo, H. Masaki, H. Sato, T. Yamaguchi, S. Hochi, T. Kobayashi, H. Nakauchi, M. Hirabayashi


【論116】 Production of human tissue-type plasminogen activator (htPA) using in vitro cultured transgenic pig mammary gland cells
Anim. Biotechnol.,30(4):317-322 2019
Author:H-J. Chung, H-J. Park, S-Y. Baek, J-K. Park, W-Y. Lee, K-W. Kim, Y-M. Jo, S. Hochi, Y-M. Kim, T-J. Choi, E-S. Cho, K-H. Cho


【論115】 FGF2 has distinct molecular functions from GDNF in the mouse germline niche
Stem Cell Rep.,10(6):1782-1792 2018
Author:K. Masaki, M. Sakai, S. Kuroki, J. Jo, K. Hoshina, Y. Fujimori, K. Oka, T. Amano, T. Yamanaka, M. Tachibana, Y. Tabata, T. Shinozawa, O. Ishizuka, S. Hochi, S. Takashima


【論114】 Haploid embryonic stem cell lines derived from andorogenetic and parthenogenetic rat blastocysts
J. Reprod. Dev.,63(6):611-616 2017
Author:M. Hirabayashi, H. Hara, T. Goto, A. Takizawa, M.R. Dwinell, T. Yamanaka, S. Hochi, H. Nakauchi


【論113】 Nylon mesh device for vitrification of large quantities of rat pancreatic islets
Biopreserv. Biobank.,15(5):457-462 2017
Author:T. Yamanaka, T. Goto, M. Hirabayashi, S. Hochi


【論112】 Downsizing cumulus cell layers to improve cryotolerance of germinal vesicle-stage bovine oocytes
Theriogenology,95:1-7 2017
Author:K. Tashima, Y. Kubo, M. Hirabayashi, S. Hochi


【論111】 Direct comparison of Cryotop® vitrification and Bicell® freezing on recovery of functional rat pancreatic islets
Cryobiology,73(3):376-382 2016
Author:T. Yamanaka, K. Tashima, R. Takahashi, S. Takashima, T. Goto, M. Hirabayashi, S. Hochi


【論110】 Hypomorphic phenotype of Foxn1 gene-modified rats by CRISPR/Cas9 system
Transgenic Res.,25(4):533-544 2016
Author:T. Goto, H. Hara, H. Nakauchi, S. Hochi, M. Hirabayashi


【論109】 Rat blastocysts from nuclear injection and time-lagged enucleation and their commitment to embryonic stem cells
Cell. Reprogram.,18(2):108-115 2016
Author:H. Hara, T. Goto, A. Takizawa, H.J. Jacob, M. Sanbo, T. Kobayashi, H. Nakauchi, S. Hochi, M. Hirabayashi


【論108】 High incidence of polyspermic fertilization in bovine oocytes matured in vitro after Cryotop vitrification
Cryo Letters,37(1):27-33 2016
Author:I-S. Hwang, D-J. Kwon, G-S. Im, K. Tashima, S. Hochi, S. Hwang


【論107】 Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries
Zygote,24(1):115-120 2016
Author:H. Hara, M. Tagiri, M. Hirabayashi, S. Hochi


【論106】 Knock-in of a histone H2B-tdTomato reporter into the Rosa26 locus allows visualization of cell nuclei in rats
Mol. Reprod. Dev.,82(12):916-917 2015
Author:T. Goto, T. Kobayashi, H. Hara, M. Sanbo, S. Hochi, H. Nakauchi, M. Hirabayashi


【論105】 High revivability of vitrified-warmed bovine oocytes during recovery culture with α-tocopherol
Reproduction,149(4):347-355 2015
Author:I. Yashiro, M. Tagiri, H. Ogawa, K. Tashima, S. Takashima, H. Hara, M. Hirabayashi, S. Hochi


【論104】 Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa
Cryobiology,68(3):354-360 2014
Author:H. Hara, M. Tagiri, I-S. Hwang, M. Takahashi, M. Hirabayashi, S. Hochi


【論103】 Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines
J. Reprod. Dev.,60(1):78-82 2014
Author:M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, S. Hochi


【論102】 Derivation of embryonic stem cell lines from parthenogenetically developing rat blastocysts
Stem Cells Dev.,23(2):107-114 2014
Author:M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, M. Kato-Itoh, H. Sato, T. Kobayashi, H. Nakauchi, S. Hochi


【論101】 Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization
Zygote,22(4):476-482 2014
Author:H. Hara, I. Yamane, I. Noto, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi


【論100】 Rescue of vitrified-warmed bovine oocytes with Rho-associated coiled-coil kinase inhibitor
Biol. Reprod.,89(2):26 1-6 2013
Author:I-S. Hwang, H. Hara, H-J. Chung, M. Hirabayashi, S. Hochi


【論99】 A retrospective analysis of germline competence in rat embryonic stem cell lines
Transgenic Res.,22(2):411-416 2013
Author:M. Hirabayashi, C. Tamura, M. Sanbo, M. Kato-Itoh, T. Kobayashi, H. Nakauchi, S. Hochi


【論98】 Proteins associated with reproductive disorders in testes of human erythropoietin gene-harboring transgenic boars
Theriogenology,78(5):1020-1029 2012
Author:M-S. Choi, M-R. Shim, M-Y. Oh, K-W. Kim, H-C. Lee, B-C. Yang, H-K. Chung, J-H. Kim, H-T. Lee, I-S. Hwang, S. Hochi, Y-T. Heo, N-H. Kim, S-J. Uhm, J-K. Park, W-K.Chang, H-J. Chung


【論97】 Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells
Mol. Reprod. Dev.,79(6):402-412 2012
Author:M. Hirabayashi, C. Tamura, M. Sanbo, T. Goto, M. Kato, T. Kobayashi, H. Nakauchi, S. Hochi


【論96】 High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization
Theriogenology,77(5):908-915 2012
Author:H. Hara, I-S. Hwang, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi


【論95】 Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center
J. Reprod. Dev.,57(3):428-432 2011
Author:H. Hara, H. Abdalla, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi


【論94】 In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification
J. Assist. Reprod. Genet.,28(4):355-361 2011
Author:H. Iwayama, S. Hochi, M. Yamashita


【論93】 Birefringent parameter available for quantitative analysis of human zona hardness
Zygote,19(4):323-329 2011
Author:H. Iwayama, S. Hochi, M. Yamashita


【論92】 Low stretching ability of human ooplasma during piezo-ICSI as a risk factor on post-injection survival and implantation
J. Mammal. Ova Res.,27(3):150-156 2010
Author:H. Iwayama, S. Hochi, M. Yamashita


【論91】 Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
Theriogenology,74(6):1028-1035 2010
Author:H. Abdalla, M. Shimoda, H. Hara, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi


【論90】 Rat transgenesis via embryonic stem cells electroporated with Kusabira-orange gene
Mol. Reprod. Dev.,77(6):474 2010
Author:M. Hirabayashi, M. Kato, M. Sanbo, T. Kobayashi,S. Hochi, H. Nakauchi


【論89】 Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
Theriogenology,73(8):1139-1145 2010
Author:S. Hochi, H. Abdalla, M. Shimoda, H. Hara, H. Morita, M, Kuwayama, M. Hirabayashi


【論88】 Establishment of rat embryonic stem cell lines that can participate into germline chimerae at high efficiency
Mol. Reprod. Dev.,77(2):94 2010
Author:M. Hirabayashi, M. Kato, T. Kobayashi, M. Sanbo, T. Yagi, S. Hochi, H. Nakauchi


【論87】 Impaired active demethylation of paternal genome in pronuclear-stage rat zygotes produced by in vitro ferilization or intracytoplasmic sperm injection
Mol. Reprod. Dev.,77(1):69-75 2010
Author:Y. Yoshizawa, M. Kato, M. Hirabayashi, S. Hochi


【論86】 A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
Theriogenology,72(4):453-460 2009
Author:H. Abdalla, M. Shimoda, M. Hirabayashi, S. Hochi


【論85】 Demethylation dynamics of paternal genome in pronuclear-stage bovine zygotes produced by in vitro fertilization and ooplasmic injection of freeze-thawed or freeze-dried spermatozoa
J. Reprod. Dev.,55(4):433-439 2009
Author:H. Abdalla, M. Hirabayashi, S. Hochi


【論84】 Activation regimens for full-term development of rabbit oocytes injected with round spermatids
Mol. Reprod. Dev.,76(6):573-579 2009
Author:M. Hirabayashi, M. Kato, K. Kitada, N. Ohnami, M. Hirao, S. Hochi


【論83】 Availability of subfertile transgenic rats expressing c-myc gene as recipients for spermatogonial transplantation
Transgenic Res.,18(1):135-141 2009
Author:M. Hirabayashi, Y. Yoshizawa, M. Kato, T. Tsuchiya, S. Nagao, S. Hochi


【論82】 The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis
Theriogenology,71(3):543-552 2009
Author:H. Abdalla, M. Hirabayashi, S. Hochi


【論81】 Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids
Exp. Anim.,57(4):401-405 2008
Author:M. Hirabayashi, M. Kato, S. Hochi


【論文80】 Effect of post-IVF developmental kinetics on in-vitro survival of vitrified-warmed domestic cat blastocysts
Reprod. Domest. Anim.,43(3):323-327 2008
Author:T.Tsujioka, C. Otzdorff, J. Braun, S. Hochi


【論79】 Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats
Exp. Anim.,57(2):145-148 2008
Author:M. Hirabayashi, M. Kato, K. Amemiya, S. Hochi


【論78】 Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
Mol. Reprod. Dev.,75(5):890-894 2008
Author:S. Hochi, K. Watanabe, M. Kato, M. Hirabayashi


【論77】 Follicle size-dependent changes of follicular fluid components and oocyte diameter in Antarctic minke whales (Balaenoptera bonaerensis)
J. Reprod. Dev.,53(6):1265-1272 2007
Author:H. Nagai, T. Mogoe, H. Ishikawa, S. Hochi, S. Ohsumi, Y. Fukui


【論76】 Attempt at intracytoplasmic sperm injection to in vitro matured oocytes in common minke whales (Balaenoptera acutorostrata) captured at Kushiro coast survey
J. Reprod. Dev.,53(4):945-952 2007
Author:Y. Fukui, H. Iwayama, T. Matsuoka, H. Nagai, N. Koma, T. Mogoe, H. Ishikawa, Y.Fujise, M. Hirabayashi, S. Hochi, H. Kato, S. Ohsumi


【論75】 Effect of enucleation on inactivation of cytostatic factor activity in matured rat oocytes
Cloning Stem Cells,9(2):257-266 2007
Author:J. Ito, M. Kato, S. Hochi, M. Hirabayashi


【論74】 The ability of whale haploid spermatogenic cells for inducing calcium oscillations and its relevance to oocyte activation
Zygote,15(2):103-108 2007
Author:K. Amemiya, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi


【論73】 Conventional freezing of in vitro produced and biopsied bovine blastocysts in the presence of low concentration glycerol with sucrose
J. Reprod. Dev.,53(2):443-447 2007
Author:K. Tominaga, F. Iwaki, S. Hochi


【論72】 Quality analysis of buffalo blastocysts derived from oocytes vitrified before and after enucleation and reconstructed with somatic cell nuclei
Theriogenology,67(4):893-900 2007
Author:S. Muenthaisong, C. Laowtammathron, M. Katudat-Cairns, R. Parnpai, S. Hochi


【論71】 Involvement of Ca(2+)-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of rat matured oocytes
Theriogenology,67(3):475-485 2007
Author:J. Ito, M. Shimada, S. Hochi, M. Hirabayashi


【論70】 No effect of recombinase-mediated DNA transfer on production efficiency of transgenic rats
Exp. Anim.,55(2):131-135 2006
Author:M. Hirabayashi, M. Kato, R. Kaneko, T. Hirabayashi, M. Morira, S. Hochi


【論69】 Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection
Zygote,14(2):89-95 2006
Author:T. Fujihira, M. Kobayashi, S. Hochi, M. Hirabayashi, H. Ishikawa, S. Ohsumi, Y. Fukui


【論68】 Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)
Zygote,14(1):45-51 2006
Author:T. Kobayashi, K. Amemiya, K. Takeuchi, T. Tsujioka, K. Tominaga, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi


【論67】 Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR
Theriogenology,65(2):288-298 2006
Author:Y. Iwanami, T. Kobayashi, M. Kato, M. Hirabayashi, S. Hochi


【論66】 Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer
J. Mammal. Ova Res.,22(3):178-184 2005
Author:K. Tominaga, Y. Hamada, S. Hochi


【論65】 Factors affecting cryosurvival of nuclear-transferred bovine and swamp buffalo blastocysts: effects of hatching stage, linoleic acid-albumin in IVC medium and Ficoll supplementation to vitrification solution
Theriogenology,64(5):1185-1196 2005
Author:C. Laowtammathron, C. Lorthongpanich, M. Ketudat-Cairns, S. Hochi, R. Parnpai


【論64】 Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA
Mol. Reprod. Dev.,70(4):422-428 2005
Author:M. Hirabayashi, M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi


【論63】 Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
Zygote,13(1):79-85 2005
Author:M. Hirabayashi, M. Kato, J. Ito, S. Hochi


【論62】 Contribution of high p34(cdc2) kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes
Reproduction,129(2):171-180 2005
Author:J. Ito, M. Hirabayashi, M. Kato, A. Takeuchi, M. Ito, M. Shimada, S. Hochi


【論61】 Effects of cryodevice type and donors' sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicle-stage
Zygote,12(4):333-338 2004
Author:H. Iwayama, S. Hochi, M. Kato, M. Hirabayashi, M. Kuwayama, H. Ishikawa, S. Ohsumi, Y. Fukui


【論60】 Acquirement of oocyte activating factor in Antarctic minke whale (Balaenopterabonaerensis) spermatogenic cells, assessed by meiosis resumption of microinseminated mouse oocytes
J. Mammal. Ova Res.,21(3):149-156 2004
Author:K. Amemiya, Y. Iwanami, T. Kobayashi, T. Terao, Y. Fukui, H. Ishikawa, S. Ohsumi, M. Hirabayashi, S. Hochi


【論59】 Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts
J. Reprod. Dev.,50(2):191-195 2004
Author:M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi


【論58】 Effect of activation regimens for rat oocytes on full-term development after round spermatid injection
Contem. Top. Lab. Anim. Sci.,43(2):13-15 2004
Author:M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi


【論57】 Production of transgenic rats by ooplasmic injection of spermatogenic cells exposed to exogenous DNA: A preliminary study
Mol. Reprod. Dev.,69(2):153-158 2004
Author:M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi, M. Hirabayashi


【論56】 Successful vitrification of pronuclear-stage rabbit zygotes by minimum volume cooling procedure
Theriogenology,61(2-3):267-275 2004
Author:S. Hochi, T. Terao, M. Kamei, M. Kato, M. Hirabayashi, M. Hirao


【論55】 Factors affecting premature chromosome condensation of cumulus cell nuclei injected into rat oocytes
J. Reprod. Dev.,49(2):121-126 2003
Author:M. Hirabayashi, M. Kato, A. Takeuchi, A. Ishikawa, S. Hochi


【論54】 Factors influencing chromosome condensation and development of cloned rat embryos
Cloning Stem Cells,5(1):35-42 2003
Author:M. Hirabayashi, M. Kato, A. Ishikawa, S. Hochi


【論53】 Rescue of infertile transgenic rat lines by intracytoplasmic injection of cryopreserved round spermatids
Mol. Reprod. Dev.,62(3):295-299 2002
Author:M. Hirabayashi, M. Kato, T. Aoto, M. Ueda, S. Hochi


【論52】 Effect of Ca(2+)/Mg(2+)-free culture condition on spontaneous first cleavage of rat oocytes
J. Reprod. Dev.,48(3):243-248 2002
Author:A. Takeuchi, M. Kato, K. Ito, K. Kimura, A. Hanada, M. Hirabayashi, S. Hochi


【論51】 Offspring derived from intracytoplasmic injection of transgenicrat sperm
Transgenic Res.,11(2):221-228 2002
Author:M. Hirabayashi, M. Kato, T. Aoto, A. Sekimoto, M. Ueda, I. Miyoshi, N. Kasai, S. Hochi


【論50】 Strontium-induced activation regimen for rat oocytes in somatic cell nuclear transplantation
J. Reprod. Dev.,47(6):407-413 2001
Author:M. Kato, M. Hirabayashi, T. Aoto, K. Ito, M. Ueda, S. Hochi


【論49】 Production of transgenic rats using young Sprague-Dawley females treated with PMSG and hCG
Exp. Anim.,50(5):365-369 2001
Author:M. Hirabayashi, K. Ito, A. Sekimoto, S. Hochi, M. Ueda


【論48】 Effects of cryopreservation of pronuclear-stage rabbit zygotes on the morphological survival, blastocyst formation, and full-term development after DNA microinjection
Mol. Reprod. Dev.,60(2):227-232 2001
Author:S. Hochi, M. Hirabayashi, M. Hirao, M. Kato, T. Kobayashi, K. Kimura, K. Hirasawa, S.P. Leibo, M. Ueda


【論47】 A comparative study on the integration of exogenous DNA into mouse, rat, rabbit, and pig genomes
Exp. Anim.,50(2):125-131 2001
Author:M. Hirabayashi, R. Takahashi, K. Ito, N. Kashiwazaki, M. Hirao, K. Mirasawa, S. Hochi, M. Ueda


【論46】 Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes
Cryobiology,42(1):69-73 2001
Author:S. Hochi, M. Akiyama, G. Minagawa, K. Kimura, A. Hanada


【論45】 フィーダー細胞なしで培養した凍結融解ES細胞の胞胚腔注入による生殖系列キメラマウスの作製
実験動物技術,35(2):99-104 2000
Author:大竹 誠一, 堰本 晃代, 伊藤 和美, 保地 眞一, 平林 真澄, 上田 正次


【論44】 Production of transgenic rabbits using centrifuged pronuclear zygotes
J. Vet. Med. Sci.,62(10):1047-1052 2000
Author:M. Hirabayashi, M. Hirao, R. Takahashi, K. Kimura, K. Hirasawa, M. Ueda, S. Hochi


【論43】 Nuclear transfer in cattle: effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes
J. Vet. Med. Sci.,62(10):1111-1113 2000
Author:S. Hochi, M. Kato, K. Ito, M. Hirabayashi, M. Ueda, A. Sekimoto, Y. Nagao, K. Kimura, A. Hanada


【論42】 ウサギ胚の採取効率と発生能に及ぼす過剰排卵誘起法の影響
実験動物技術,35(1):23-28 2000
Author:平林 真澄, 平尾 雅郎, 保地 眞一, 木村 建, 平澤 和男, 上田 正次


【論41】 Effects of timing of oocyte cryopreservation on in vitro development of nuclear-transferred bovine zygotes
Mol. Reprod. Dev.,54(1):81-85 1999
Author:K. Ito, M. Hirabayashi, M. Ueda, Y. Nagao, K. Kimura, A. Hanada, S. Hochi


【論40】 Effect of time interval between biopsy and vitrification on survival of in vitro-produced bovine blastocysts
J. Reprod. Dev.,45(5):351-355 1999
Author:K. Ito, A. Sekimoto, M. Hirabayashi, S. Hochi, K. Kimura, M. Ueda, Y. Nagao


【論39】 Effect of linoleic acid-albumin in the culture medium on freezing sensitivity of in vitro-produced bovine morulae
Theriogenology,52(3):497-504 1999
Author:S. Hochi, K. Kimura, A. Hanada


【論38】 Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes
J. Mammal. Ova Res.,16(1):19-22 1999
Author:S. Hochi, A. Kanamori, K. Sugisawa, K. Kimura, A. Hanada


【論37】 Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage
Theriogenology,50(7):1093-1100 1998
Author:K. Ito, S. Otake, M. Hirabayashi, S. Hochi, M. Ueda


【論36】 Effects of percoll washing and glycosaminoglycans on the lysophosphatidylcholin induced acrosomal reaction in stallion
J. Equine Sci.,9(4):101-106 1998
Author:M. Kobayashi, M.P.B. Wijayagunawardane, Y.H. Choi, A.Y. Kojima, S. Hochi, N. Oguri, M. Takagi, K. Miyazawa, K. Sato


【論35】 Effect of nuclear stages during IVM on the survival of vitrified-warmed bovine oocytes
Theriogenology,49(4):787-796 1998
Author:S. Hochi, K. Ito, M. Hirabayashi, M. Ueda, K. Kimura, A. Hanada


【論34】 ガラス化保存したウシ卵母細胞の胚盤胞発育と凍害保護物質の希釈工程との関係
日本胚移植学雑誌,19(2):95-100 1997
Author:保地 眞一, 金森 明, 伊藤 和美, 平林 真澄, 上田 正次, 木村 建, 花田 章.


【論33】 In vitro fertilizing ability of bovine oocytes frozen-thawed at immature, maturing, and mature stages
J. Mammal. Ova Res.,14(1):61-65 1997
Author:S. Hochi, A. Kanamori, K. Kimura, A. Hanada


【論32】 Direct transfer of equine blastocysts frozen-thawed in the presence of ethylene glycol and sucrose
Theriogenology,46(7):1217-1224 1996
Author:S. Hochi, K. Maruyama, N. Oguri


【論31】 Effect of cooling and warming rates during cryopreservation on survival of in vitro-produced bovine embryos
Theriogenology,46(5):837-847 1996
Author:S. Hochi, E. Semple, S.P. Leibo


【論30】 In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification
Cryobiology,33(3):300-310 1996
Author:S. Hochi, M. Kozawa, T. Fujimoto, E. Hondo, J. Yamada, N. Oguri


【論29】 In vitro capacitation of stallion spermatozoa assessed by the lysophoshatidylcholine-induced acrosome reaction and the penetration rate into in vitro-matured, zona-free mare oocytes
J. Equine Vet. Sci.,16(6):244-248 1996
Author:S. Hochi, K. Korosue, Y.H. Choi, N. Oguri


【論28】 Activation of glutathione transferase P gene by lead requires glutathione transferase P enhancer I
J. Biol. Chem.,271(3):1626-1632 1996
Author:T. Suzuki, S. Morimura, M.B. Diccianni, R. Yamada, S. Hochi, M. Hirabayashi, A. Yuki, K. Nomura, T. Kitagawa, M. Imagawa, M. Muramatsu


【論27】 Large equine blastocysts are damaged by vitrification procedures
Reprod. Fertil. Dev.,7(1):113-117 1995
Author:S. Hochi, T. Fujimoto, N. Oguri


【論26】 Relationship among seminal characteristics, fertility, and suitability for semen preservation in Draft stallions
J. Vet. Med. Sci.,57(2):225-229 1995
Author:F. Torres-Boggino, K. Sato, A. Oka, Y. Kanno, S. Hochi, N. Oguri, J. Braun


【論25】 Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa
Cryobiology,32(5):487-492 1995
Author:J. Braun, S. Hochi, N. Oguri, K. Sato, F. Torres-Boggino


【論24】 Tissue specific activation of tumor marker glutathione transferase P transgenes in transgenic rats
J. Can. Res. Clin. Oncol.,121(9-10):606-611 1995
Author:T. Suzuki, M. Imagawa, K. Nomura, S. Hochi, M. Hirabayashi, M. Ueda, T. Kitagawa, M. Muramatsu


【論23】 An attempt to produce acellular embryonic capsule in equine blastocysts cultured in vitro
J. Reprod. Dev.,41(4):367-371 1995
Author:S. Hochi, T. Azuma, K. Miyaushiro, N. Oguri


【論22】 Effect of glucose in the culture medium on development of horse oocytes matured and microfertilized in vitro
Reprod. Fertil. Dev.,7(5):1067-1071 1995
Author:T. Azuma, Y.H. Choi, S. Hochi, N. Oguri


【論21】 ウマ先体脱落精子を評価する染色方法の比較
日本胚移植研究会誌,1(1):4-9 1994
Author:頃末 憲治, 保地 真一, 小栗 紀彦


【論20】 Influence of sera on in vitro hatching of equine blastocysts
J. Reprod. Dev.,40(1):13-18 1994
Author:S. Hochi, J. Braun, A. Miyamoto, Y. Fukui, N. Oguri


【論19】 Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezing
Theriogenology,41(4):809-818 1994
Author:J. Braun, M. Sakai, S. Hochi, N. Oguri


【論18】 Follicular development, progesterone and estradiol 17-β levels during spontaneous and FSH-stimulated estrous cycles in Hokkaido Native pony and Thoroughbred mares
J. Reprod. Dev.,40(2):141-147 1994
Author:J. Braun, Y. Moriuchi, S. Nakagawa, S. Hochi, A. Miyamoto, N. Oguri


【論17】 Pregnancies following transfer of equine embryos cryopreserved by vitrification
Theriogenology,42(3):483-488 1994
Author:S. Hochi, T. Fujimoto, J. Braun, N. Oguri


【論16】 Effect of seminal plasma on motion characteristics of epididymal and ejaculated stallion spermatozoa during storage at 5˚C
Deutsche Tierärztliche Wochenschrift,101(8):319-322 1994
Author:J. Braun, F. Torres-Boggino, S. Hochi, N. Oguri


【論15】 Influence of relative embryonic volumes during glycerol equilibration on the survival of frozen-thawed equine blastocysts
J. Reprod. Dev.,40(3):243-249 1994
Author:S. Hochi, M. Ogasawara, J. Braun, N. Oguri


【論14】 In vitro fertilization rate of horse oocytes with partially removed zonae
Theriogenology,42(5):795-802 1994
Author:Y.H. Choi, Y. Okada, S. Hochi, J. Braun, K. Sato,N. Oguri


【論13】 2歳齢の北海道和種牝馬における胚の採取
日本胚移植研究会誌,1(3):169-174 1994
Author:伊藤 博史, 保地 真一, ヨアヒム ブラウン, 小栗 紀彦


【論12】 Cryopreservation of equine oocytes by 2-step freezing
Theriogenology,42(7):1085-1094 1994
Author:S. Hochi, T. Fujimoto, Y.H. Choi, J. Braun, N. Oguri


【論11】 Effects of individual stallions and mare breeds on assisted fertilization in vitro
J. Equine Sci.,5(3):77-81 1994
Author:S. Hochi, Y.H. Choi, K. Korosue, K. Sato, N. Oguri


【論10】 Trans-activation of glutathione transferase P gene during chemical hepatocarcinogenesis of the rat
Proc. Natl. Acad. Sci. USA,90(3):2065-2068 1993
Author:S. Morimura, T. Suzuki, S. Hochi, A. Yuki, K. Nomura, T. Kitagawa, I. Nagatsu, M. Imagawa, M. Muramatsu


【論9】 The hatching process of equine embryos in vitro
Equine Vet. J. Suppl.,15:91-94 1993
Author:S. Hochi, J. Braun, N. Oguri


【論8】 In vitro maturation of equine oocytes collected by follicle aspiration and by the slicing of ovaries
Theriogenology,40(5):959-966 1993
Author:Y.H. Choi, S. Hochi, J. Braun, K. Sato, N. Oguri


【論7】 ウマ卵胞卵子の採取効率と体外成熟率に影響する要因
日本ウマ科学会雑誌,4(2):145-150 1993
Author:保地 真一, 崔 龍鎬, ヨアヒム ブラウン, 佐藤 邦忠, 小栗 紀彦


【論6】 Cryoprotective effect of polyols on rat embryos during two-step freezing
Cryobiology,29(3):332-341 1992
Author:K. Utsumi, S. Hochi, A. Iritani


【論5】 Secretion of bovine α-lactalbumin into the milk of transgenic rats
Mol. Reprod. Dev.,33(2):160-164 1992
Author:S. Hochi, T. Ninomiya, M. Waga-Honma, J. Sagara, A. Yuki


【論4】 Fate of exogenous DNA carried into mouse eggs by spermatozoa
Anim. Biotechnol.,1(1):21-31 1990
Author:S. Hochi, T. Ninomiya, A. Mizuno, M. Honma, A. Yuki


【論3】 DNA breaks and repair in the mouse leukemia L1210 cells exposed to three types of interstrand DNA cross-linkers
Mutat. Res.,236(1):43-50 1990
Author:N. Yajima, S. Hochi, N. Miyata, T. Kishi, G. Kawanishi


【論2】 Successful production of transgenic rats
Anim. Biotechnol.,1(2):175-184 1990
Author:S. Hochi, T. Ninomiya, M. Honma, A. Yuki


【論1】 凍結ラット胚の生存性に対する血清の効果
家畜人工授精研究会誌,8(1):2-5 1986
Author:保地 真一, 内海 恭三, 入谷 明, 湯原 正高


その他
【総説17】 多能性幹細胞を用いた三次元臓器再生 –胚盤胞補完の齧歯類モデル–
BIO Clinica , 34(12):25-29 2019
Author:平林 真澄, 保地 眞一


【総説16】 Pluripotent stem cell-derived organogenesis in the rat model system
Transgenic Res. , 28(3-4):287-297 2019
Author:M. Hirabayashi, T. Goto, S. Hochi


【総説15】 Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies
Anim. Sci. J. , 87(9):1076-1083 2016
Author:S. Hochi


【総説14】 Recent progress in cryopreservation of bovine oocytes
BioMed Res. Int. , 2014:570647 1-11 2014
Author:I-S. Hwang, S. Hochi


【総説13】 Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in rats
Reprod. Med. Biol. , 10(4):231-238 2011
Author:M. Hirabayashi, S. Hochi


【総説12】 Challenging endeavour for preservation of freeze-dried mammalian spermatozoa
J. Reprod. Dev. , 57(5):557-563 2011
Author:S. Hochi, H. Abdalla, H. Hara, M. Hirabayashi


【総説11】 Active demethylation of paternal genome in mammalian zygotes
J. Reprod. Dev. , 55(4):356-360 2009
Author:H, Abdalla, Y. Yoshizawa, S. Hochi


【総説10】 ラットのICSI (卵細胞質内精子注入法) を利用して -外来遺伝子の導入ならびに精子の凍結乾燥-
実験動物技術 , 43(1):51-56 2007
Author:平林 真澄, 保地 眞一


【総説9】 Transgenesis via intracytoplasmic sperm injection (ICSI) in rodents
J. Mammal. Ova Res. , 23(3):86-90 2006
Author:M. Hirabayashi, M. Kato, S. Hochi


【総説8】 特集:ラットにおける顕微授精技術
LABIO 21 , (23):9-14 2006
Author:平林 真澄, 保地 眞一


【総説7】 ラットにおける顕微授精技術
実験動物技術 , 39(1):13-18 2004
Author:平林 真澄, 保地 眞一


【総説6】 Cryopreservation of follicular oocytes and preimplantation embryos in cattle and horses
J. Reprod. Dev. , 49(1):13-21 2003
Author:S. Hochi


【総説5】 Maintenance of transgenes in rats: the contributions of embryo cryopreservation and intracytoplasmic sperm/spermatid injection
J. Reprod. Dev. , 48(3):205-213 2002
Author:S. Hochi, M. Hirabayashi


【総説4】 ウシ未受精卵ならびに前核期卵の超低温保存
日本胚移植学雑誌 , 22(1):17-22 2000
Author:保地 眞一


【総説3】 リノール酸アルブミンによる体外受精由来ウシ胚の耐凍性増強効果
日本胚移植学雑誌 , 21(2):101-105 1999
Author:保地 眞一


【総説2】 Current status of IVM/IVF/IVC technology and embryo cryopreservation in the equine species
J. Mammal. Ova Res. , 15(3):117-127 1998
Author:S. Hochi


【総説1】 ウマ胚の初期発生とカプセル
家畜繁殖技術研究会誌 , 15(3):199-204 1993
Author:保地 真一

研究費
科学研究費補助金(研究代表者)
2016 - 2019 , 複雑な細胞集合構造体 (ウシ卵丘卵子複合体・ラット膵島) の保存技術開発 , 基盤研究
2012 - 2015 , 凍結乾燥したウシ精子ならびに二倍体細胞からの胚盤胞作製システム , 基盤研究
2008 - 2011 , 凍結乾燥ウシ精子の同種および異種顕微授精系による評価 , 基盤研究
2001 - 2002 , 形質転換ウサギ作製のための前核期卵の凍結保存技術の開発 , 基盤研究

科学研究費補助金(研究分担者)
2005 - 2007 , テロメレース高発現系トランスジェニックラットからの未分化細胞株の樹立 , 萌芽研究
2004 - 2006 , 体細胞核の細胞周期に応じたクローンラット作製方法の開発 , 基盤研究
2003 - 2007 , 脳機能解析に用いるノックアウトラットの開発 , 特定領域研究
2000 - 2002 , 産学連携による遺伝子操作動物・発生工学技術支援ネットワークの構築 , 地域連携推進研究
2000 - 2001 , ウシ及びウマにおける除核未受精卵の保存と核移植に関する研究 , 基盤研究
研究諸活動
学術専門雑誌等編集
2008- , Theriogenology (Elsevier Inc.)
2008- , J. Reprod. Dev. (IPEC Inc.)

学術論文査読件数
2005- , Theriogenology / J. Reprod. Dev. / Sci. Rep. / Biol. Reprod. / Human Reprod. / Reproduction / Mol. Reprod. Dev. / Cryobiology / Anim. Reprod. Sci. / Reprod. Domest. Anim. / J. Assist. Reprod. Genet.等. 計32誌, 186論文 (2019年9月1日現在)

教育活動実績

授業等
2019 , 前期 , 応用生物学への招待 / 学部1年 (1/15 x 2)
2019 , 前期 , 安全教育 / 学部2年 (1/10)
2019 , 後期 , 生殖工学特論 / 博士1年
2019 , 後期 , 細胞工学 / 学部3年
2019 , 後期 , 科学英語演習 / 学部3年 (5/15)
2019 , 後期 , 家畜生産学特論 / 修士1年
2019 , 後期 , 動物生理学 / 学部2年