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IHARA　Masaki

Academic OrganizationAcademic Assembly　School of Science and Technology　Institute of AgricultureTEL
Education and Research OrganizationInterdisciplinary Cluster for Cutting Edge Research　Institute for Biomedical SciencesFAX
PositionAssociated ProfessorMail Addressm_ihara＠shinshu-u.ac.jp
Address8304 Minamiminowa, Nagano, 399-4511, JapanWeb sitehttps://www.masakiihara.info/
https://mihara40.wixsite.com/sustainabilitylab/

Modified:04/12/2022

Profile

Assigned Class
Faculty of Agriculture　Department of Agricultural and Life Sciences　Division of Bioscience and Biotechnology
Research Field
Algae
Carbon fixation
Protein Engineering
Metabolic Engineering
Academic Societies
Academic Societies
日本蛋白質科学会
日本生物工学会
日本農芸化学会
Academic Background
Graduate School
京都大学 , 2002

College
岡山大学 , 1996

Degree
工学博士
Awards
2006 , GSCポスター賞
2006 , GRC Green Chemistry selected poster presenter
Research Career
Research Career
2002-2004 , 日本学術振興機構特別研究員

Research

Books, Articles, etc.
Books
光合成の改造と、エネルギー生産系への挑戦, 人工光合成 実用化に向けた最新技術 , 270-278
情報機構 2013
Author:伊原正喜・河野祐介


天然光合成の改造と、エネルギー物質生産藻類の創製, 化学工業 63 , 31-38
化学工業社） 2012
Author:伊原正喜・河野祐介


人工光合成とエネルギー変換材料への転換, バイオサイエンスシリーズ「光合成のエネルギー・物質変換　人工光合成を目指して」
2012
Author:伊原正喜


Develpment of energy producing phototropha, Kagaku Keizai 58 , 93
2011
Author:M. Ihara


ヒドロゲナーゼの安定化・酸素耐性化, 信州大学農学部紀要
2011
Author:伊原正喜


第三の免疫測定法「オープンサンドイッチ法」の開発, 化学工学 72 , 486-488
2008
Author:伊原正喜・上田宏


水素を作るたんぱく質, 化学と教育誌 56 , 32-35
2008
Author:伊原正喜


光合成微生物の水素発生能の効率化, ケミカルバイオエンジニアリング 52 , 249-257
2007
Author:伊原正喜


Articles
Production of extracellular polysaccharides and phycobiliproteins from Tolypothrix sp. PCC7601 using mechanical milking systems
2020
Author:Alice Uchida, Yukiko Higashi, Shota Yamamoto, Jun Nakanishi, Naoki Kanayama, Kazuhiro Shibata, Masaki Ihara


A novel Mucidosphaerium sp. from Beppu hot spring down-regulates inflammatory gene expression in skin and articular cells
2019
Author:Mitsuyoshi Miyata, Shunsuke Iwata, Chie Mifude, Kazuyuki Matsushima, Masahiro Tajima, Mayu Kameyama, Masaki Ihara, Takanori Matsui, Sho-ichi Yamagishi, Hiroyuki Ishitobi, Shigeru Miyaki, Kuniyoshi Kaseda


Overexpression of endogenous 1-deoxy-D-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation
Journal of Bioscience and Bioengineering,123(5):590-596 2017
Author:Kai Kudoh, Shingo Hotta, Midori Sekine, Arisu Uchida, Genma Kubota, Rintaro Fujii, Yusuke Kawano, Masaki Ihara


Exploration of the 1-deoxy-D-xylulose 5-phosphate synthases suitable for the creation of a robust isoprenoid biosynthesis system
Journal of Bioscience and Bioengineering,123(3):300-307 2017
Author:Kudoh Kai, Kubota Genma, Fujii Rintaro, Kawano Yusuke, Ihara Masaki*


Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
Applied Microbiology and Biotechnology,101(1):341-349 2017
Author:Shigemori S, Ihara M, Sato T, Yamamoto Y, Nigar S, Ogita T, Shimosato T
WebofScienceリポジトリ電子ジャーナル


生物触媒を利用した半人工光合成
化学と工業,69(11):963-965 2016
Author:伊原正喜


Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice
Microbial Cell Factories,14:189 2015
Author:Suguru Shigemori, Takafumi Watanabe, Kai Kudoh, Masaki Ihara, Shireen Nigar, Yoshinari Yamamoto, Yoshihito Suda, Takashi Sato, Haruki Kitazawa* and Takeshi Shimosato*


Expression Systems for Soluble Metal-Dependent Formate Dehydrogenase
Journal of Photochemistry & Photobiology, A: Chemistry 2015


Enhancement of L-cysteine production by disruption of yciW in Escherichia coli
Journal of Bioscience and Bioengineering,119:176-179 2015


Simultaneous retention of thermostability and specific activity in chimeric human alkaline phosphatases
Mol. Biotechnol,56:953-961 2015


微細藻類によるバイオエネルギー及びバイオマテリアル生産
月刊技術士,595(9):12-15 2014
Author:伊原正喜


Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-D-xylulose 5-phosphate synthase and carbon allocation analysis
Journal of Bioscience and Bioengineering,118:20-28 2014


Identification and characterization of UDP-glucose pyrophosphorylase in Anabaena sp. PCC 7120
Journal of Bioscience and Bioengineering,117:531-538 2014


Expression of an antibody-enzyme complex by the L-chain fusion method
Journal of Bioscience and Bioengineering,116:17-21 2013
Author:Chungyong Han, Masaki Ihara, and Hiroshi


A protein-protein interaction assay based on the functional complementation of mutant firefly luciferases
Analytical Chemistry,85:7985-7940 2013


Light Driven CO2 Fixation by Using Cyanobacterial Photosystem I and NADPH-Dependent Formate Dehydrogenase
PLOS ONE,8(8):e71581 2013
Author:Ihara, M; Kawano, Y; Urano, M; Okabe, A
WebofScienceリポジトリ電子ジャーナル


Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments
Bioconjugate Chemistry,23:1463-1469 2012
Author:Koichi Minami, Masaki Ihara, Shou Kuroda, Hirohiko Tsuzuki, and Hiroshi Ueda


“Quenchbodies”: quench-based antibody probes that show antigen-dependent fluorescence
J. Am. Chem. Soc.,133:17386 2011
Author:R. Abe, H. Ohashi, I. Iijima, M. Ihara, H. Takagi, T. Hohsaka and H. Ueda


Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules
Bioconjugate chemistry,:633-641 2011
Author:Kojima et al.


Direct construction of open-sandwich enzyme immunoassay for one-step noncompetitive detection of thyroid hormone T4
Analytical Chemistry,83:1008-1014 2011
Author:Islam et al.


Expression of antibody variable region-human alkaline phosphatase fusion proteins in mammalian cells
Journal of Immunological Methods,361:57-63 2010
Author:SasaJima et al.


Micro open-sandwich ELISA to rapidly evaluate thyroid hormone concentration from serum samples
Bioanalysis,2:1683-1687 2010
Author:Islam et al.


Antibody affinity maturation in vitro using unconjugated peptide antigen
Protein Engineering Design and Selection,23:185-193 2010
Author:Iwai et al.


Antibody affinity maturation in vitro using unconjugated peptide antigen
Lab on a Chip,10:92-100 2010
Author:Ihara et al.


Open-sandwich enzyme immunoassay for one-step noncompetitive detection of corticosteroid 11-deoxycortisol
Analytical Chemistry,81:8298-8304 2009
Author:Ihara et al.


Open sandwich-based immuno-transistor for label-free and non-competitive detection of low molecular weight antigen
Analytical Chemistry,81:7532-7537 2009
Author:Sakata et al.


Trans-splicing as a novel method to rapidly produce antibody fusion proteins
Biochemical and Biophysical Research Communications,384:316-321 2009
Author:Iwasaki et al.


Bovine Insulin Filaments Induced by Reducing Disulfide Bonds Show a Different Morphology, Secondary Structure and Cell Toxicity from Intact Insulin Amyloid Fibrils
Biophysical Journal,96:3331-3340 2009
Author:Zako et al.


Antibody Fab display system that can perform open sandwich ELISA
Analytical Biochemistry,386:36-44 2009
Author:Dong et al.


Photoinduced Hydrogen Production by Direct Electron Transfer from Photosystem I Cross-linked with Cytochrome c3 to [NiFe]-Hydrogenase
Photochemical andPhotobiology,82:1677-1685 2006
Author:Ihara et al.


Design of Artificial Hydrogenase-Photosystem I Complex for Photo-driven Hydrogen Production by Cyanobacteria or Microalga
Photochemical and Photobiology,82:676-682 2006
Author:Ihara et al.


Functions of fluctuation in the heme-binding loops of cytochrome b5 revealed in the process of heme incorporation
2000
Author:Masaki Ihara, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima


Conversion of an electron-transfer protein into an oxygen binding protein: The axial cytochrome b5 mutant with an unusually high O2 affinity
2000
Author:Masaki Ihara, Masato Shintaku, Satoshi Takahashi, Koichiro Ishimori, Isao Morishima


MISC
天然の光合成を凌駕した光水素生産バイオナノデバイス
月刊化学,(6月号) 2012
Author:伊原正喜

Patents
Patents
酸化還元酵素と光合成反応中心との連結体による光駆動物質生産法
目的物質の測定方法
蛍光免疫測定方法
分子構造の再現方法
断片化抗体固定化担体及びその製造方法
抗体を選別するためのベクター
抗体と蛋白質との融合蛋白質の製造方法
抗チロキシン抗体及びそれを用いた免疫測定方法
断片化抗体固定化担体及びその製造方法
分子間の相互作用の検出方法
抗原検出方法及び抗原検出装置
Themes for Joint Research
Phycoerythrin production by microalgae
Molecular Model
Predetor-tolerant microalgae
Oil degradation by photosensitizing effect of chlorophyll
CO2-fixation by bacteria
Research Grants
Joint Research
2017 - 2019 , ギ酸デヒドロゲナーゼを用いた新規人工炭酸固定系
2013 - 2016 , ヒドロゲナーゼの改良を目指した分子進化工学的手法の開発
2009 - 2015 , タンパク質工学によるギ酸生産藻類の創出
2006 - 2009

Grants‐in‐aid for Scientific Research(Research Representative)
2009 - 2014 , 蛋白質工学によるギ酸生産藻類の創製

Education

Lectures
外書講読
有機分析化学
有機分析化学演習
Thesis Guidance
2010
Graduation Thesis Number of Students Total：3(International Students:3)

Social Activities

Social Activitie
Extension Courses
201010 - 201010 , サイエンスカフェ , 信州大学農学部・日本学術会議化学委員会・日本農芸化学会・東京応化科学技術振興財団の共催で行った