Sakurai, Takayuki | Shinshu University Researcher Directory

Academic Organization	Academic Assembly　School of Medicine and Health Sciences　Institute of Medicine	TEL
Education and Research Organization	Interdisciplinary Cluster for Cutting Edge Research　Institute for Biomedical Sciences	FAX
Position	Associate Professor	Mail Address	tsakurai＊shinshu-u.ac.jp
Address	3－1－1Asahi,Matsumoto,390-0621,Japan	Web site	http://www7a.biglobe.ne.jp/~shindo/

Profile

Research Field: Developmental Biology
Developmental Biothechnology
Cardiovascular Biology
Genome Editing
Keywords:Developmental engineering , Genome Editing , Developmental biology , Transgenic animal

Current Subject: Understanding of Adrenomedullin/Ramps functions that are involved in cardiovascular diseases　and inflammation
Keywords:Adrenomedullin , Ramp , Cardiovascular , Inflammation
Generation and characterization of new mouse models of human diseases

Development of new technologies needed for generating gene-modified animal

Academic Societies: Academic Societies
The Molecular Biology Society of Japan
The Japanese Society for Regenerative Medicine
The Japanese Society for Genome Editing

Academic Background: Graduate School
Grad School Yokohama City Univ , 1995
Grad School Shizuoka Univ , 1992

College
Shizuoka Univ , 1990

Degree
Doctor of Science , Grad School Yokohama City Univ
Master of Science , Grad School Shizuoka Univ

Research Career: Research Career
2019- , Associate Professor, Department of Life Innovation, Institute for Biomedical Sciences, Shinshu University
2008- , Associate Professor Grad School Med　Shinshu Univ
2005-2008 , Senior Assistant Professor Grad School Med　Shinshu Univ
1999-2005 , Assistant Professor Schooi of Med Tokai Unicersity

Research

Books, Articles, etc.: Books
Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line, Microinjection: Methods and Protocols Methods in Molecular Biology , Chapter 15
Springer Nature 2019
Author:Takayuki Sakurai, Akiko Kamiyoshi, Masato Ohtsuka, Channabasavaiah B Gurumurthy, Masahiro Sato, Takayuki Shindo

Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals, Results and Problems in Cell Differentiation 63 , Chapter 21
Springer 2017
Author:Takayuki Sakurai, Takayuki Shindo, Masahiro Sato

アドレノメデュリン-RAMP2シグナル, 細胞工学 35 1, 33-38
2016
Author:新藤隆行、桜井敬之、神吉昭子、市川優佳、田中愛、小山晃英

アドレノメデュリンによる血管形成の調節-遺伝子操作動物における検討ー, 医学のあゆみ 223 13, 1063-1068
医歯薬出版 2007
Author:新藤隆行　桜井敬之

マウスの卵母細胞と着床前胚に対するRNAi実験入門, RNAi A GUIDE TO GENE SILENCING, 303-333
メディカルサイエンスインターナショナル 2004
Author:桜井敬之　木村穣 (訳）

Articles
Receptor activity modifying protein RAMP sub-isoforms and their functional differentiation, which regulates functional diversity of adrenomedullin
Biology,11(5):788 2022
Author:Shindo T, Tanaka M, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Sakurai T

An Myh11 single lysine deletion causes aortic dissection by reducing aortic structural integrity and contractility
Sci Rep,12(1):8844 2022
Author:Negishi K, Aizawa K, Shindo T, Suzuki T, Sakurai T, Saito Y, Miyakawa Y, Tanokura M, Kataoka Y, Maeda M, Shota Tomida, Hiroyuki Morita, Takeda N, Komuro I, Kario K, Nagai R, Imai Y

Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing
STAR Protoc,2(2) 2021
Author:Sakurai T, Shindo T

Adrenomedullin Ameliorates Pulmonary Fibrosis by Regulating TGF-ß-Smads Signaling and Myofibroblast Differentiation
Endocrinology,162(8):1-22 2021
Author:Wei Y, Tanaka M, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Cui N, Kakihara S, Zhao, Y, Aruga K, Sanjo H, Shindo T

Adrenomedullin-RAMP2 and -RAMP3 Systems Regulate Cardiac Homeostasis during Cardiovascular Stress
Endocrinology,162(3):1-20 2021
Author:Cui N, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Tanaka M, Wei Y, Kakihara S, Zhao Y, Aruga K, Kawagishi H, Nakada T, Yamada M, Shindo T

Adrenomedullin-RAMP2 system ameliorates subretinal fibrosis by suppressing epithelial-mesenchymal transition in age-related macular degeneration
Am J Pathol,191(4):652-668 2021
Author:Tanaka M, Kakihara S, Hirabayashi K, Imai A, Toriyama Y, Iesato Y, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Cui N, Wei Y, Zhao Y, Aruga K, Yamauchi A, Murata T, Shindo T

Deficiency of the adrenomedullin-RAMP3 system suppresses metastasis through the modification of cancer-associated fibroblasts.
Oncogene,39(10):1914-1930 2020
Author:Dai, K.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Matsui, S.; Shindo, T.

Production of genetically engineered mice with higher efficiency, lower mosaicism, and multiplexing capability using maternally expressed Cas9.
Sci.Rep,10(1):1091 2020
Author:Sakurai, T.; Kamiyoshi, A.; Kawate, H.; Watanabe, S.; Sato, M.; Shindo, T.

Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice.
Heliyon,5:e02655 2019
Author:Yamauchi, A.; Kamiyoshi, A.; Sakurai, T.; Miyazaki, H.; Hirano, E.; Lim, HS.; Kaku, T.; Shindo, T.

Endogenous calcitonin gene-related peptide deficiency exacerbates postoperative lymphedema by suppressing lymphatic capillary formation and M2 macrophage accumulation.
Am J Pathol.,,189:2487-2502 2019
Author:Matsui, S.; Tanaka, M.; Kamiyoshi, A.; Sakurai, T.; Ichikawa-Shindo, Y.; Kawate, H.; Dai, K.; Cui, N.; Wei, Y.; Tanaka, M.; Kakihara, S.; Nakamura, K.; Yamauchi, A.; Ishida, K.; Tanaka, S.; Kawamata, M.; Shindo, T.

Development of a novel model of central retinal vascular occlusion and the therapeutic potential of the adrenomedullin-receptor activity-modifying protein 2 system.
Am J Pathol.,,189:449-466 2019
Author:Hirabayashi, K.; Tanaka, M.; Imai, A.; Toriyama, Y.; Iesato, Y.; Sakurai, T.; Kamiyoshi, A.; Ichikawa-Shindo, Y.; Kawate, H.; Tanaka, M.; Dai, K.; Cui, N.; Wei, Y.; Nakamura, K.; Iida, S.; Matsui, S.; Yamauchi, A.; Murata, T.; Shindo, T.

Transplacental delivery of genome editing components causes mutations in embryonic cardiomyocytes of mid-gestational murine fetuses.
IUBMB Life,71(7):835-844 2019
Author:Nakamura S, Ishihara M, Ando N, Watanabe S, Sakurai T, Sato M

Regulation of cardiovascular development and homeostasis by the adrenomedullin-RAMP system
Peptides,111:55-61 2019
Author:Shindo T, Tanaka M, Kamiyoshi, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Sakurai T

Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
Sci Rep,9(1):9923 2019
Author:Sakurai T, Kamiyoshi A, Takei N, Watanabe S, Sato M, Shindo T.

Endogenous calcitonin gene-related peptide suppresses ischemic brain injuries and progression of cognitive decline.
J Hypertens,36(4):876-891 2018
Author:Zhai L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Xian X, Hirabayashi K, Dai K, Cui N, Tanimura K, Liu T, Wei Y, Tanaka M, Tomiyama H, Yamauchi A, Igarashi K, Shindo T.

Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Theriogenology,108:29-38 2018
Author:Sato M, Kosuke M, Koriyama M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K.

The Combinational Use of CRISPR/Cas9 and Targeted Toxin Technology Enables Efficient Isolation of Bi-Allelic Knockout Non-Human Mammalian Clones.
Int J Mol Sci.,19(4):E1075 2018
Author:Watanabe S, Sakurai T, Nakamura S, Miyoshi K, Sato M.

In vivo genome editing targeted towards the female reproductive system.
Arch Pharm Res.,41(9):898-910 2018
Author:Sato M, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Gurumurthy CB

RAMP3 deficiency enhances postmenopausal obesity and metabolic disorders
Peptides,110:10-18 2018
Author:Liu T, Kamiyoshi A, Tanaka M, Iida S, Sakurai T, Ichikawa-Shindo Y, Kawate H, Hirabayashi K, Dai K, Cui N, Tanaka M, Wei Y, Nakamura K, Matsui S, Yamauchi A, Shindo T

Endogenous Calcitonin Gene-Related Peptide Regulates Lipid Metabolism and Energy Homeostasis in Male Mice.
Endocrinology.,158(5):1194-1206 2017
Author:Liu T, Kamiyoshi A, Sakurai T, Ichikawa-Shindo Y, Kawate H, Yang L, Tanaka M, Xian X, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.

Vasoprotective Activities of the Adrenomedullin-RAMP2 System in Endothelial Cells.
Endocrinology.,158(5):1359-1372 2017
Author:Xian X, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Tanaka M, Koyama T, Kawate H, Yang L, Liu T, Imai A, Zhai L, Hirabayashi K, Dai K, Tanimura K, Liu T, Cui N, Igarashi K, Yamauchi A, Shindo T.

Adrenomedullin Suppresses Vascular Endothelial Growth Factor-Induced Vascular Hyperpermeability and Inflammation in Retinopathy.
Am J Pathol.,187(5):999-1015 2017
Author:Imai A, Toriyama Y, Iesato Y, Hirabayashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Tanaka M, Liu T, Xian X, Zhai L, Dai K, Tanimura K, Liu T, Cui N, Yamauchi A, Murata T, Shindo T.

Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Int J Mol Sci.,18(12):E2610 2017
Author:Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A.

A non-inheritable maternal Cas9-based multiple-gene editing system in mice
Sci.Rep,6:srep20011 2016
Author:Sakurai T, Kamiyoshi A, Kawate H, Mori C, Watanabe S, Tanaka M, Uetake R, Sato M, Shindo T

Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques
Sci.Rep,6:srep35861 2016
Author:Uemura T, Mori T, Kurihara T, Kawase S, Koike R, Satoga M, Cao X, Li X, Yanagawa T, Sakurai T, Shindo T, Tabuchi K

Rac1-Mediated Activation of Mineralocorticoid Receptor in Pressure Overload-Induced Cardiac Injury
Hypertension,67:99-106 2016
Author:Ayuzawa N, Nagase M, Ueda K, Nishimoto M, Kawarazaki W, Marumo T, Aiba A, Sakurai T, Shindo T, Fujita T

The piggyBac-Based Gene Delivery System Can Confer Successful Production of Cloned Porcine Blastocysts with Multigene Constructs
,17:E1424 2016
Author:Sato M, Maeda K, Koriyama M, Inada E, Saitoh I, Miura H, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S, Miyoshi K

The endothelial adrenomedullin-RAMP2 system regulates vascular integrity and suppresses tumour metastasis
Cardiovasc.Res,111:398-409 2016
Author:Tanaka M, Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Liu T, Xian X, Imai A, Zhai L, Hirabayashi K, Owa S, Yamauchi A, Igarashi K, Taniguchi S, Shindo T

Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations
Int.J.Mol.Sci,16:17838-17856 2016
Author:Sato M, Koriyama M, Watanabe S, Ohtsuka M, Sakurai T, Inada E, Saitoh I, Nakamura S, Miyoshi K

Generation of α-1,3-Galactosyltransferase-Deficient Porcine Embryonic Fibroblasts by CRISPR/Cas9-Mediated Knock-in of a Small Mutated Sequence and a Targeted Toxin-Based Selection System
Reprod Domest Anim,50:872-880 2015
Author:Sato M, Kagoshima A, Saitoh I, Inada E, Miyoshi K, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S.

A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
Biotechnol J,10:143-153 2015
Author:Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S

Adrenomedullin-RAMP2 System in Vascular Endothelial Cells
J Atheroscler Thromb,22:647-653 2015
Author:Koyama T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Shindo T

Pathophysiological function of endogenous calcitonin gene-related peptide in ocular vascular diseases
Am J Pathol,185:1783-1794 2015
Author:Toriyama Y, Iesato Y, Imai A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Igarashi K, Tanaka M, Liu T, Xian X, Zhai L, Owa S, Murata T, Shindo T

Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Biotechnol J,8:1355-1361 2014
Author:Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Xenotransplantation,21:291-300 2014
Author:Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

Adrenomedullin-RAMP2 System Suppresses ER Stress-Induced Tubule Cell Death and Is Involved in Kidney Protection
PLoS ONE,9(2):e87667 2014
Author:Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T

The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts
Xenotransplantation,21:291-300 2014
Author:Sato M, Miyoshi K, Nagao Y, Nishi Y, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

Pathophysiological roles of adrenomedullin-RAMP2 system in acute and chronic cerebral ischemia
Peptides,62:21-31 2014
Author:Igarashi K, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yamauchi A, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T

A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
BMC Biotechnol,14:69 2014
Author:Sakurai T, Watanabe S, Kamiyoshi A, Sato M, Shindo T

Functional differentiation of RAMP2 and RAMP3 in their regulation of vascular system
J Mol Cell Cardiol,77:73-85 2014
Author:Yamauchi A, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Igarashi K, Toriyama Y, Tanaka M, Liu T, Xian X, Imai A, Zhai L, Owa S, Arai T, Shindo T

Adrenomedullin-RAMP2 system suppresses ER stress-induced tubule cell death and is involved in kidney protection
PLoS One,9:e87667 2014
Author:Uetake R, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Yoshizawa T, Koyama T, Yang L, Toriyama Y, Yamauchi A, Igarashi K, Tanaka M, Kuwabara T, Mori K, Yanagita M, Mukoyama M, Shindo T

Regulation of Adrenomedullin and its Family Peptide by RAMP System - Lessons from Genetically Engineered Mice
CURRENT PROTEIN & PEPTIDE SCIENCE,14(5):347-357 2013
Author:Shindo, T; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Shimoyama, N; Iinuma, N; Arai, T; Miyagawa, S

Vascular endothelial adrenomedullin-RAMP2 system is essential for vascular integrity and organ homeostasis
Circulation.,127(7):842-853 2013
Author:Koyama T, Ochoa-Callejero L, Sakurai T ,Kamiyoshi A, Ichikawa-Shindo Y, Iinuma N, Arai　T, Yoshizawa T, Iesato Y, Lei Y, Uetake R, Okimura A, Yamauchi A, Tanaka M, Igarashi K, Toriyama Y, Kawate H, Adams RH, Kawakami H, Mochizuki N, Martínez A, Shindo T

Novel regulation of cardiac metabolism and homeostasis by the adrenomedullin-receptor activity-modifying protein 2 system
Hypertension,61(2):341-351 2013
Author:Yoshizawa T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Iesato Y, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Nakada T, Kashihara T, Yamada M, Kawakami H, Nakanishi H, Taguchi R, Nakanishi T, Akazawa H, Shindo T.

Endogenous CGRP protects against neointimal hyperplasia following wire-induced vascular injury
J Mol Cell Cardiol.,59:55-66 2013
Author:Yang L, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T..

Adrenomedullin-RAMP2 system is crucially involved in retinal angiogenesis.
Am J Pathol.,182(6):2380-2390 2013
Author:Iesato Y, Toriyama Y, Sakurai T,, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Uetake R, Yang L, Yamauchi A, Tanaka M, Igarashi K, Murata T, Shindo T

Regulation of adrenomedullin and its family peptide by RAMP system--lessons from genetically engineered mice
Curr Protein Pept Sci.,14(5):347-357 2013
Author:Shindo T, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Shimoyama N, Iinuma N, Arai T, Miyagawa S.

Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Biotechnol J.,8(11):1355-1361 2013
Author:Sato M, Inada E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

Toxin-Based Selectable Drug-Free Enrichment of Mammalian Cells with High Transgene Expression
Biology,2:341-355 2013
Author:Sato M Akasaka E Saitoh I Ohtsuka M Nakamura S Sakurai T, Watanabe S

Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study
Advances in Stem Cells,2013(2013):ID 208514 2013
Author:Sato M Kubota N Inada E Saitoh I Ohtsuka M Nakamura S Sakurai T Watanabe S

A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates
J. Biomedical Science and Engineering,5:406-408 2012
Author:Sato M, Akasaka E, Saitoh I, Ohtsuka M, Nakamura S, Sakurai T, Watanabe S

Induction of LYVE-1/stabilin-2 -positive liver sinusoidal endothelial-like cells from embryoid bodies by modulation of adrenomedullin-RAMP2 signaling.
Peptides,32:1855-1865 2011
Author:Arai T, Sakurai T, Kamiyoshi A, Shindo Y, Iinuma N, Iesato Y, Koyama T, Yoshizawa T, Uetake R, Yamauchi A, Yang L, Kawate H, Ogawa S, Kobayashi A, Miyagawa S, Shindo T

Improvement of a phiC31 integrase-based gene delivery system that confers high and continuous transgene expression
N Biotechnol,28:312-319 2011
Author:Watanabe S; Nakamura S; Sakurai T; Akasaka K; Sato M

A novel glycosylation signal regulates transforming growth factor b receptors as evidenced by endo-b-galactosidase C expression in rodent cells
Glycobiology,21:486-492 2011
Author:Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Sato M

Adrenomedullin in sinusoidal endothelial cells play protective roles against cold injury of liver
PEPTIDES,31(5):865-871 2010
Author:Iinuma, N; Sakurai, T; Kamiyoshi, A; Ichikawa-Shindo, Y; Arai, T; Yoshizawa, T; Koyama, T; Uetake, R; Kawate, H; Muto, S; Tagawa, Y Miyagawa, S; Shindo, T

Development of CRTEIL and CETRIZ, Cre-loxP-based system, whichi allow change of expression of red to green or green to red fluorescence upon transfection with a cre-expression vector
J Biomed Biotechnol,2009(2009):985140 2009
Author:Ohtsuka M; Warita T; Sakurai T; Watanabe S; Inoko H; Sato M

Endogenous alpha-calcitonin gene-related peptide mitigates liver fibrosis in chronic hepatitis induced by repeated administration of concanavalin A.
Liver Int,29(5):642-649 2009
Author:Kamiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Iinuma N; Kawate, H; Yoshizawa T; Koyama T; Muto, S; Shindo, T

Production and characterization of transgenic mice systemically expressing endo-beta-galactosidase C.
Glycobiology,18(1):9-19 2008
Author:Watanabe S; Misawa M; Matsuzaki T; Sakurai T; Muramatsu T; Yokomine TA; Sato M

The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity.
J Clin Invest,118(1):29-39 2008
Author:Ichikawa-Shindo Y; Sakurai T; Kamiyoshi A; Kawate H et al.,

Rapid zygosity determination in mice by SYBR Green real-time genomic PCR of a crude DNA solution
Transgenic Res,17(1):149-155 2008
Author:Sakurai T; Kamiyoshi A; Watanabe S; Sato M: Shindo T

Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,364(1):14-19 2007
Author:Nakanishi T; Kumagai S; Kimura M; Watanabe H; Sakurai T; Kimura M;Kashiwabara S; Baba T

Testatin transgenic and knockout mice exhibit normal sex-differentiation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,341(2):369-375 2006
Author:Hasegawa, K; Chuma, S; Tada, T; Sakurai, T; Tamura, M; Suemori, H; Nakatsuji, N

Folliculogenesis following syngeneic transplantation of young murine ovaries into the testes.
Reprod Med Bio,5(1):71-77 2006
Author:Sato M: Sakurai T; Kiryu K; Takeda M; Yasuoka Y

Strain difference in tolerance to low-temperature treatment of fertilized mouse oocytes.
Reprod Med Bio,5(1):43-50 2006
Author:Sakurai T; Watanabe S; Sato M: Kimura M

Azelnidipine, a long-acting dihydropyridine calcium channel blocker, suppresses the development of cardiac hypertrophy and fibrosis in adrenomedullin knock out mice.
CIRCULATION,114(18):13-13 2006
Author:Shindo, T; Niu, P; Kamiyoshi, A; Sakurai, T; Ichikawa, Y; Iinuma, N; Kawate, H

Preparationof enzymatically active human Myc tagged NCre recombinase exhibiting immunoreactivity with anti Myc antibody
Mol Reprod Dev,73(11):1345-1352 2006
Author:Watanabe S; Honnma D; Furusawa T; Sakurai T; Sato M

Endogenous alpha CGRP protects against concanavalin A-induced hepatitis in mice
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,343(1):152-158 2006
Author:Kainiyoshi, A; Sakurai, T; Ichikawa-Shindo, Y; Fukuchi, J; Kawate, H; Muto, S; Tagawa, Y; Shindo, T

Temporal developmental arrest after storage of fertilized mouse eggs at 4oC: Effects on embryonic development, maternal mRNA processing and cell cycle
MORECULAR HUMAN REPRODUCTION,11(5):325-333 2005
Author:Sakurai T; Sato M: Kimura M

Divers patterns of poiy(A) tail elongation and shortning of murinematernal mRNAs from fully-grown oocyte to 2-cell embryo stages
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,336(4):1181-1189 2005
Author:Sakurai T; Sato M: Kimura M

A novel method for constructing murine cDNA library enriched with maternal mRNAs exhibiting de novo independent post-fertilization polyadenylation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,327(3):688-699 2005
Author:Sakurai T; Sato M: Kimura M

Patents: Patents
トランスジェニック動物の作製方法、トランスジェニック動物
DNAフラグメント及び医薬組成物

Research Grants: Cooperative research with industry
2015 - 2016 , 生理活性因子の情報制御システムに基づく、革新的な医薬品の創出, AMED-CREST
2013 - 2014 , 受容体活性調節タンパクＲＡＭＰを標的とした創薬科学技術振興機構研究成果最適展開支援プログラム

Grants‐in‐aid for Scientific Research(Research Representative)
2019 - 2021 , 体系的複数同時ゲノム編集法を応用したRAMP1、2、3の機能分化と相互作用の解明 , 基盤研究 (C)
2016 - 2017 , 「母性Cas9」による多遺伝子編集マウス開発と多因子性疾患の解明 , 萌芽研究
2014 - 2017 , 次世代遺伝子改変技術を応用した炎症性腸疾患におけるR2・R3の機能連関の解明 , 基盤研究（B）
2014 - 2016 , 体系的・多遺伝子同時改変マウス作製法開発によるアドレノメデュリンRAMP機能解明 , 萌芽研究
2011 - 2014 , アドレノメデュリン-RAMP2・RAMP3システムの破綻が惹起する炎症病態の解明 , 基盤研究（Ｃ）
2008 - 2010 , 多機能に関わるアドレノメデュリンーＲＡＭＰシステムの発生工学的病態解析と治療検討 , 基盤研究（Ｃ）
2006 - 2007 , キメラマウスによる多機能ペプチド、アドレノメデュリンと関連因子の全組織包括的解析 , 基盤研究（Ｃ）
2004 - 2005 , マウス受精卵で転写を伴わないポリA鎖伸長を示す母性RNA群の多様性と生物学的意義 , 基盤研究（Ｃ）
2000 - 2001 , マウス受精卵における時期特異的ポリ(A)鎖伸長母性RNA群の系統的解明 , 奨励研究（A)

Grants‐in‐aid for Scientific Research(Researcher)
2021 - 2023 , 受容体活性調節システムによる生体内恒常性制御機構の解明と創薬への展開 , 基盤研究（B）
2020 - 2020 , 血管およびリンパ管の恒常性制御は、癌転移抑制法となりうるか？ , 挑戦的研究(萌芽)
2018 - 2021 , 癌特異的代謝を介し増悪化に寄与するGPCR OR7C1の作用機序解明と治療応用 , 基盤研究(C)
2015 - 2015 , 創薬研究の加速に向けた、次世代遺伝子編集技術の開発 JST研究成果展開事業　マッチングプランナープログラム｢探索試験｣
2014 - 2017 , 臓器間連携と恒常性を司る、新しい生体内情報制御システムの解明と応用展開 , 基盤研究（B）
2009 - 2011 , 創薬シーズ開発の効率化に向けた次世代疾患モデルマウスの迅速作製技術開発 , JST A-Step 研究成果最適化支援事業
2008 - 2010 , RAMPによる代謝、血管制御メカニズムの解明と、代謝異常、血管合併症治療への展開 , 基盤研究（B）
2003 - 2005 , マウス初期胚における細胞質RNAの動的変化の重要性の解明 , 基盤研究（B）
2001 - 2001 , 受精後の初期発生プログラムに連動するポリA鎖伸長母性RNAの網羅的アプローチ , 特定領域研究（C）
2000 - 2000 , マウス初期胚母性RNAの時期特異的ポリ(A)鎖伸長の意義 , 特定領域研究（A)
1996 - 1997 , マウス着床前初期胚における遺伝子発現の総合的解析 , 国際学術

Other Research Grants
2021 - 2021 , アイバワークス医学研究者助成
2017 - 2017 , 信州医学振興会
2011 - 2011 , 内藤記念科学奨励金血管統合機構の解明と治療応用
2009 - 2009 , アストラゼネカグラントリサーチ2009
2006 - 2006 , 三井生命医学研究助成　アドレノメデユリンーRAMP2システムを標的とした､メタポリックシンドローム､動脈硬化症の新規治療法開発
2006 - 2006 , アストラゼネカグラントリサーチ2006

Education

Lectures: Introduction to Medical Science
Molecular Embryology
Graft Adaptation

Shinshu University Researcher Directory

Sakurai Takayuki

Profile

Research

Education

Sakurai　Takayuki