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TAKASHIMA Seiji

Academic OrganizationAcademic Assembly School of Science and Technology Institute of Textile Science and TechnologyTEL0268-21-5344
Education and Research OrganizationFaculty of Textile Science and Technology Department of Applied BiologyFAX
PositionAssociate ProfessorMail Addressstakashi@shinshu-u.ac.jp
Address3-15-1, Tokida, Ueda City 386-8567Web site

Profile

Research Field
Regenerative medicine
Reproductive biology
Experimental medicine
Current Subject
Biology of spermatogonial stem cell
Keywords:Spermatogonial stem cell , Germline stem cell , self-renewal , differentiation , ageing
Pluripotency potential of germline stem cell
Keywords:Germline stem cell , Pluripotency , Dedifferentiation , reprograming
Aging of stem cell system
Keywords:aging , stem cell , niche , organ-organ crosstalk , cytokine
Academic Societies
Academic Societies
ISSCR
The Society for Reproduction and Development
Academic Background
Graduate School
2004

College
2001
1999
Awards
2015
Research Career
Research Career
2019-
2018-
2014-
2007-
2004-

Research

Books, Articles, etc.
Articles
(原著)Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
Scientific Reports,10:10796 2020(Jul. 01)
Author:Akihiro Tsuchimoto, Masaaki Tone, Narumi Ogonuki, Masashi Hada, Atsuo Ogura & Seiji Takashima


(原著)FGF2 has distinct molecular functions from GDNF in the mouse germline niche.
Stem Cell Reports,10(6):1782-1792 2018(Apr. 19)
Author:Masaki K, Sakai M, Kuroki S, Jo J, Hoshina K, Fujimori Y, Oka K, Amano T, Yamanaka T, Tachibana M, Tabata Y, Shiozawa T, Ishizuka O, Hochi S, Takashima S.


(原著)Expression dynamics of self-renewal factors for spermatogonial stem cells in the mouse testis.
J. Reprod. Dev.,64(3):267-275 2018(Apr. 16)
Author:Sakai M, Masaki K, Aiba S, Tone M, Takashima S.


(総説)Culture and transplantation of spermatogonial stem cells.
Stem Cell Reports,29:46-55 2018(Mar. 15)
Author:Takashima, S., Shinohara, T.


(総説)Biology and manipulation technologies of male germline stem cells in mammals.
Reprod. Med. Biol.,In Press 2018
Author:Takashima, S.


(総説/日本語)哺乳類精子幹細胞の試験管内操作技術.
日本胚移植学雑誌,39:143-149 2017(Aug. 05)
Author:高島誠司


(原著)Direct comparison of Cryotop® vitrification and Bicell® freezing on recovery of functional rat pancreatic islets.
Cryobiology,73(3):376-382 2016(Dec. 01)
Author:Yamanaka T, Tashima K, Takahashi R, Takashima S, Goto T, Hirabayashi M, Hochi S.


(国内学会発表受賞記念要旨収録)Differential expression of FGF2 and GDNF in the mouse testis
J Reprod. Dev. ,62(1):A8 2016(Feb. 20)
Author:Yagi, M., Takashima, S.


(原著)Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal.
Stem Cell Reports,4(3):489-502 2015(Mar. 10)
Author:Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Morimoto, H., Inoue, K., Ogonuki, N., Jijiwa, M., Takahashi, M., Ogura, A., Shinohara, T.


(原著)High revivability of vitrified-warmed bovine mature oocytes after recovery culture with α-tocopherol.
Reproduction,149(4):347-355 2015(Jan. 27)
Author:Yashiro, I., Tagiri, M., Ogawa, H., Tashima, K., Takashima, S., Hara, H., Hirabayashi, M., Hochi, S.


(原著)Regulation of pluripotency in male germline stem cells by Dmrt1
Genes Dev.,27:1949-1958 2013
Author:Takashima, S., Hirose, M., Ogonuki, N., Ebisuya, M., Inoue, K., Morimoto, H., Kanatsu-Shinohara, M., Nishida, E., Ogura, A., Shinohara, T.


(原著)Reconstitution of mouse spermatogonial stem cell niches in culture
Cell Stem Cell,11:567-578 2012
Author:Kanatsu-Shinohara, M., Inoue, K., Takashima, S., Takehashi, M., Ogonuki, N., Morimoto, H., Nagasawa, T., Ogura, A., Shinohara, T.


(原著)Dynamic changes in EpCAM expression during spermatogonial stem cell differentiation in the mouse testis
PLos One,6:e23663 2011
Author:Kanatsu-Shinohara, M., Takashima, S., Ishii. K., Shinohara, T.


(原著)Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier
Cell Stem Cell,9:463-475 2011
Author:Takashima, S., Kanatsu-Shinohara, M., Tanaka, T., Takehashi, M., Morimoto, H., Shinohara, T.


(原著)Genetic influences in mouse spermatogonial stem cell self-renewal
J. Reprod. Dev,56:145-153 2010
Author:Kanatsu-Shinohara, M., Ogonuki, N., Miki, H., Inoue, K., Morimoto, H., Takashima, S., Ogura, A., Shinohara, T.


(原著)Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation
Proc. Natl. Acad. Sci. USA,107:6210-6215 2010
Author:Kanatsu-Shinohara, M., Takashima, S., Shinohara, T.


(原著)Alpha 1 antitrypsin activity is decreased in human amnion in premature rupture of the fetal membranes
Mol. Hum. Reprod.,15:49-57 2009
Author:Izumi-Yoneda, N., Toda, A., Okabe, M., Koike, C., Takashima, S., Yoshida, T., Konishi, I., Saito, S., Nikaido, T.


(原著)Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
Biol. Reprod.,81:155-164 2009
Author:Takashima, S., Takehashi, M., Lee, J., Chuma, S., Okano, M., Hata, K., Suetake, I., Nakatsuji, N., Miyoshi, H., Tajima, S., Tanaka, Y., Toyokuni, S., Sasaki, H., Kanatsu-Shinohara, M., Shinohara, T.


(原著)Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation
Cell Stem Cell,5:76-86 2009
Author:Lee, J., Kanatsu-Shinohara, M., Morimoto, H., Kazuki, Y., Takashima, S., Oshimura, M., Toyokuni, S., Shinohara, T.


(原著)Phenotypic plasticity of mouse spermatogonial stem cells
Plos One,4:e7909 2009
Author:Morimoto, H., Kanatsu-Shinohara, M., Takashima, S., Chuma, S., Nakatsuji, N., Takehashi, M., Shinohara, T.


(原著)Characterization of laminin isoforms in human amnion
Tissue Cell,40:75-81 2008
Author:Takashima, S., Yasuo, M., Sanzen, N., Sekiguchi, K., Okabe, M., Yoshida, T., Toda, A., Nikaido, T.


(原著)Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells
Biol. Reprod.,79:1121-1128 2008
Author:Kanatsu-Shinohara, M., Kato, M., Takehashi, M., Morimoto, H., Takashima, S., Chuma, S., Nakatsuji, N., Hirabayashi, M., Shinohara, T.


(原著)Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin
Cell Stem Cell,3:533-542 2008
Author:Kanatsu-Shinohara, M., Takehashi, M., Takashima, S., Lee, J., Morimoto, H., Chuma, S., Raducanu, A., Nakatsuji, N., Fässler, R., Shinohara, T.


(原著)Human amniotic epithelial cells possess hepatocyte-like characteristics and functions
Cell Struct. Funct.,29:73-84 2004
Author:Takashima, S., Ise, H., Zhao, P., Akaike, T., Nikaido, T.


(原著)Transplanted human amniotic epithelial cells express connexin 26 and Na-K-ATPase in the inner ear
Transplantation,77:1452-1454 2003
Author:Yuge, I., Takumi ,Y., Koyabu, K., Hashimoto, S., Takashima, S., Fukuyama, T., Nikaido, T., Usami, S.


(原著)Analysis of cell viability and differential activity of mouse hepatocytes under 3D and 2D culture in agarose gel
Biotechnol. Lett.,21:209-213 1999
Author:Ise, H., Takashima, S., Nagaoka, M., Ferdous, A., Akaike, T.


Presentations
(国際学会発表)“Dysregulated interleukin-1 activity results in male infertility”
2017(Oct. 31)
Presenter:Kaito Masaki, Shunta Sakanishi, Shigeru Kyuwa, Shigeru Kakuta, and Seiji Takashima


(国際学会発表)“Depletion of recipient germ cells compromises the spermatogenesis of transplanted testis tissue”
2017(Sep. 27)
Presenter:Akihiro Tsuchimoto, Masaaki Tone and Seiji Takashima


(国際学会発表)“The role of neuroendocrine system in regulating spermatogenesis”
2017(Sep. 27)
Presenter:Shota Aiba, Haruhiko adachi, Kaito Masaki and Seiji Takashima


(国際学会発表)“Ambivalent behavior of fibroblast growth factor 2 in mouse germline niche”
2017(Sep. 27)
Presenter:Kaito Masaki, Shunsuke Kuroki, Jun-ichiro Jo, Makoto Tachibana, Yasuhiko Tabata, Seiji Takashima


(国際学会発表/ポスター)“Differential expression of self-renewal factors for spermatogonial stem cells in the mammalian testicular niche”
ISSCR annual meating 2017(Jun. 15)
Presenter:Seiji Takashima, 、Kaito Masaki、Jun-ichiro Jo, and Yasuhiko Tabata


(国内学会発表・ポスター)FGF2による未分化型精原細胞制御/日本実験動物学会
日本実験動物学会 2017(May 17)
Presenter:(高島誠司),正木魁人、城潤一郎,田畑泰彦


(国内学会発表・口頭)精子幹細胞自己複製因子 FGF2 の精巣内での役割/日本畜産学会第122回大会
日本畜産学会 2017(Mar. 29)
Presenter:(高島誠司),正木魁人


(国内学会発表/ポスター)抗ガン剤Busulfanが精子幹細胞ニッシェへ及ぼす負の影響/第109回日本繁殖生物学会
日本繁殖生物学会 2016(Sep. 12)
Presenter:刀根 将晃 高島 誠司


(国内学会発表/ポスター)精巣機能制御における末梢神経の役割の解明/第109回日本繁殖生物学会
日本繁殖生物学会 2016(Sep. 12)
Presenter:足立晴彦, 八木瑞貴, 高島誠司


(国内学会発表/ポスター)ヒト雄性不妊病態モデルの解析/第109回日本繁殖生物学会
日本繁殖生物学会 2016(Sep. 12)
Presenter:正木魁人、八木瑞貴、角田茂、坂西俊太、久和茂、高島誠司


(国内学会発表・口頭)2つの精子幹細胞自己複製因子の精巣内発現動態と機能の比較/第109回日本繁殖生物学会
日本繁殖生物学会 2016(Sep. 12)
Presenter:高島誠司、正木魁人、黒木俊介、立花誠、保地眞一、八木瑞貴


(国際学会発表/ポスター)“Differential expression of self-renewal factors for spermatogonial stem cells in the mammalian testicular niche”
ISSCR annual meating 2016(May 24)
Presenter:Seiji Takashima, Mizuki Yagi, Kaito Masaki, Shunsuke Kuroki, Makoto Tachibana, Yuki Fujimori, Kazuo Hoshina, Kenji Oka, Toshiyasu Amano, Tanri Shiozawa, Osamu Ishizuka and Shinichi Hochi


(国内学会発表・口頭)2つの精子幹細胞自己複製因子の精巣内差次的発現とその制御メカニズムの解析/アンドロロジー学会
日本アンドロロジー学会 2016(May 24)
Presenter:正木魁人、八木瑞貴、黒木俊介、立花誠、藤森祐紀、保科和夫、岡賢二、天野俊康、塩沢丹里、石塚修、保地眞一、高島誠司


(国内学会発表・口頭)2つの精子幹細胞因子GDNFとFGF2の差次的発現の意義/再生医療学会
日本再生医療学会 2016(Mar. 19)
Presenter:八木瑞貴、正木魁人、黒木俊介、立花誠、藤森祐紀、保科和夫、岡賢二、天野俊康、塩沢丹里、石塚修、保地眞一、高島誠司


(国内学会発表・口頭)精子幹細胞が示す2つの増殖様式/再生医療学会
日本再生医療学会 2016(Mar. 18)
Presenter:高島誠司


(国際学会発表)“Differential expression of spermatogonial self-renewal factors in the mouse testis”
2016(Feb. 18)
Presenter:Mizuki Yagi, Haruhiko Adachi, Masaaki Tone, Kaito Masaki, Yamato Kawahara, Hayato Ogawa and Seiji Takashima


(国際学会発表)“Glial cell line-derived neurotrophic factor independent self-renewal of murine spermatogonial stem cells”
2016(Feb. 18)
Presenter:Seiji Takashima, Mito Kanatsu-Shinohara, Takashi Tanaka, Hiroko Morimoto, Kimiko Inoue, Narumi Ogonuki, Mayumi Jijiwa, Masahide Takahashi, Atsuo Ogura and Takashi Shinohara


(国際学会発表)“Dmrt1 suppress the pluripotency of male germline stem cells”
2016(Feb. 18)
Presenter:Seiji Takashima, Michiko Hirose, Narumi Ogonuki, Miki Ebisuya, Kimiko Inoue, Hiroko Morimoto, Mito Kanatsu-Shinohara, Atsuo Ogura and Takashi Shinohara


(国際学会発表/ポスター)“Differential expression of spermatogonial self-renewal factors in the mouse testis”
International Symposium on Epigenome Dynamics and Regulation in Germ Cell P69 2016(Feb. 17)
Presenter:Mizuki Yagi, Haruhiko Adachi, Masaaki Tone, Kaito Masaki, Yamato Kawahara, Hayato Ogawa and Seiji Takashima


(国際学会発表/ポスター)“Glial cell line-derived neurotrophic factor independent self-renewal of murine spermatogonial stem cells”
International Symposium on Epigenome Dynamics and Regulation in Germ Cell P60-2 2016(Feb. 17)
Presenter:Seiji Takashima, Mito Kanatsu-Shinohara, Takashi Tanaka, Hiroko Morimoto, Kimiko Inoue, Narumi Ogonuki, Mayumi Jijiwa, Masahide Takahashi, Atsuo Ogura and Takashi Shinohara


(国際学会発表/ポスター)“Dmrt1 suppress the pluripotency of male germline stem cells”
International Symposium on Epigenome Dynamics and Regulation in Germ Cell P60-1 2016(Feb. 17)
Presenter:Seiji Takashima, Michiko Hirose, Narumi Ogonuki, Miki Ebisuya, Kimiko Inoue, Hiroko Morimoto, Mito Kanatsu-Shinohara, Atsuo Ogura and Takashi Shinohara


(国内学会発表・ポスター・優秀発表賞受賞)精子幹細胞自己複製因子FGF2の精巣内発現挙動解析/第108回日本繁殖生物学会
2015(Sep. 18)
Presenter:八木瑞貴、高島誠司


(国内学会発表・口頭)精子幹細胞が有する潜在的分化多能性の分子メカニズム/第108回日本繁殖生物学会
2015(Sep. 18)
Presenter:高島誠司


(招待講演)精子幹細胞の新しい自己複製様式
第80回日本インターフェロン・サイトカイン学会 2015(Jul. 16)
Presenter:高島誠司


(招待講演)アステラス病態代謝研究会 研究報告会
2014(Oct. 18)
Presenter:高島誠司


(国内学会発表・口頭)精子幹細胞が有する潜在的分化多能性の分子メカニズム/第07回日本繁殖生物学会
2014(Aug. 22)
Presenter:高島誠司


(招待講演)培養精子幹細胞が有する潜在的分化多能性
第45回精子研究会 2014(Jan. 11)
Presenter:高島誠司


(プロシーディング)“Alpha 1 antitypsin activity in preterm premature rupture of amniotic membranes”
PLACENTA 28 8-9 A37 2007
Presenter:Yoneda, N., Toda, A., Okabe, M., Takashima, S., Yoshida, T., Konishi, I., Saito, S., Nikaido, T.


(国際学会発表)“Human amnion derived cells show characteristics of stem cell and hepatocyte,”
The 1st international symposium on developmental biology and tissue engineering 2003
Presenter:Takashima, S., Zhao, P., Akaike, T., and Nikaido, T.


(国際学会発表)“3-D culture of hepatocytes in agarose gel for application to Bio-Artificial Liver system,”
6th world biomaterials congress 2000
Presenter:Takashima, S., Ise, H., and Akaike, T.,


(国際学会発表)“Hepatocytes forming spheroid in agarose gel survive from apoptosis and maintain cell viability and expression of specific function for long-term,”
4th international conference on cellular engineering, 1999
Presenter:Takashima, S., Ise, H., and Akaike, T.

Research Grants
Joint Research
2015 - , 精子幹細胞のバイオロジー
2015 - , 精子幹細胞のバイオロジー
2015 - , 精子幹細胞のバイオロジー
2015 - , 精子幹細胞のバイオロジー
2014 - , 家畜精子幹細胞の培養法確立
2014 - , ヒト精子幹細胞培養法の確立
2014 - , ヒト精子幹細胞培養法の確立
2014 - , ヒト精子幹細胞培養法の確立
2014 - , ヒト精子幹細胞培養法の確立
2014 - , 精子幹細胞のバイオロジー
2014 - , 精子幹細胞のバイオロジー
2014 - , 精子幹細胞のバイオロジー
Research Activities
Journal Reviewer
2017-2017 , J. Reprod. Dev
2016-2016 , Scientific reports
2016-2016 , J. Reprod. Dev
2015-2015 , JARG
2015-2015 , J. Reprod. Dev
2015-2015 , J. Reprod. Dev

Education

Lectures
2017
2017
2017
2017
2016
2016
2016
2016
2015
2015
2015
2015
2014
2014
2014
2014
Thesis Guidance
2017
Graduation Thesis Number of Students Total:2(International Students:0)
Master's Thesis Number of Students Total:2(International Students:0)
Doctor's Thesis Number of Students Total:0
Researcher's Thesis Number of Students Total:0
Special Researcher's Thesis Number of Students Total:0

2016
Graduation Thesis Number of Students Total:3(International Students:0)
Master's Thesis Number of Students Total:2(International Students:0)
Doctor's Thesis Number of Students Total:0(International Students:0)
Researcher's Thesis Number of Students Total:0(International Students:0)
Special Researcher's Thesis Number of Students Total:0(International Students:0)

2015
Graduation Thesis Number of Students Total:2(International Students:0)
Master's Thesis Number of Students Total:1(International Students:0)
Doctor's Thesis Number of Students Total:0(International Students:0)
Researcher's Thesis Number of Students Total:0(International Students:0)
Special Researcher's Thesis Number of Students Total:0(International Students:0)

2014
Graduation Thesis Number of Students Total:2(International Students:0)
Master's Thesis Number of Students Total:0(International Students:0)
Doctor's Thesis Number of Students Total:0(International Students:0)
Researcher's Thesis Number of Students Total:0(International Students:0)
Special Researcher's Thesis Number of Students Total:0(International Students:0)

Social Activities

Social Activitie
Organization of Conferences and/or Symposia
20180912 - 20180916 , 日本繁殖生物学会第111回上田大会 , 事務局長

Media Appearances
20150213 , 関西テレビ , 京都大学 新たな「精子幹細胞」を発見 , http://www.ktv.jp/news/date/20150213.html
20150213 , 京都新聞(2月13日 31面) , 別タンパク質で精子幹細胞増殖 京大グループ解明 , http://www.kyoto-np.co.jp/education/article/20150213000043
20150213 , 産経新聞(2月13日 30面) , 新型の「精子幹細胞」を発見 , http://www.sankei.com/life/news/150213/lif1502130018-n1.html
20150213 , 日刊工業新聞(2月16日 21面) , 京大など、増殖の仕方が異なる2種類のGS細胞の存在を確認-男性不妊治療に活用へ , http://www.nikkan.co.jp/news/nkx1020150216eaaa.html
20150213 , 日本経済新聞(2月13日 42面) , 男性不妊症の治療に光 京大、精子の幹細胞に別タイプ発見 , http://www.nikkei.com/article/DGXLZO83125460T10C15A2CR8000/
20150213 , 読売新聞(2月13日夕刊 3面) , 不明
20150213 , KBS京都 , 京大チーム、発見 男性不妊症の治療に光 , http://webcache.googleusercontent.com/search?q=cache:QtmF8n4UOscJ:www.kbs-kyoto.co.jp/contents/news/2015/02/post-11934.htm+&cd=7&hl=ja&ct=clnk&gl=jp&client=safari
20150209 , 京都大学広報 , 精子幹細胞の新しい自己複製様式の発見 -男性不妊症の原因解明、遺伝病の発症機序の理解に期待- , http://www.kyoto-u.ac.jp/ja/research/research_results/2014/150213_1.html