Shinshu University Researcher Directory｜Shinshu University Online system of General Academic Resources SOAR

Academic Organization	Academic Assembly　School of Science and Technology　Institute of Textile Science and Technology	TEL	+81-268-21-5333
Education and Research Organization	Faculty of Textile Science and Technology　Department of Applied Biology	FAX
Position	professor	Mail Address	moriwaki＠shinshu-u.ac.jp
Address	3-15-1 Tokita,Ueda, Nagano 386-8567	Web site	https://www.ftst.jp/bi9/wordpress/

Profile

Research Field: Environmental dynamic analysis
Environmental impact assessment
Environmental engineering and reduction of environmental burden
Analytical chemistry
Green/Environmental chemistry
Environmental policy and social systems
Keywords:Environment Measurement , Influence Evaluation Technique , Environmentl Restoration Technology , Green Chemistry , Environmental Analysis , Instrumental Analysis

Current Subject: Environmental Influence Valuation
Keywords:urine , 8-OHdG , metabolism , 2004-
Environmental Analysis
Keywords:LC/MS , ICP/MS , core sample , wastes , 2000-
Decomposition of pollutants
Keywords:sonochemistry , photochemistry , 1994-

Academic Societies: Academic Societies
The Mass Spectrometry Society of Japan
Japan Society on Water Environment
Japan Society for Environmental Chemistry
The Japan Society for Analytical Chemistry

Academic Background: Graduate School
Osaka University , (Engineering , Department of Applied Chemistry) , 1996
Osaka University , (Engineering , Department of Applied Chemistry) , 1993

College
Osaka University , (Department of Applied Chemistry) , 1991

Degree
Dr. (Engineering) , Osaka University

Research Career: Research Career
2015- , Shinshu University; professor
2007- , Shinshu University; associate professor
1996-2007 , Osaka City Institute of Public Health&Environment Sciences; researcher

Overseas Education
2004-2005 , Institute of Cancer Research in UK

Research

Books, Articles, etc.: Books
質量分析学, 88-93
日本質量分析学会 2016
Author:豊田岐聡、荒川隆一、中村健道、石濱泰、角井伸次、森脇　洋
Abstract:The basic principles and application of liquid chromatography/ mass spectrometry have been described.
Keywords:液体クロマトグラフィー質量分析法

Waste Management in Japan, 109-117
WIT press 2004(Sep.)
Author:H. Itoh (Editor), Y. Yoshida, Y. Ishii, Y. Ito, M.Uebori, K. Kawata,　M. Takino, A. Hasegawa, H. Fukui, M. Murakami, H. Moriwaki, J. Yonekubo and S.Suzuki　
Abstract:What kind of chemical substances may be contained in illegally disposed wastes can not be known; however in order to verify their environmental safety, there is much need for them to be analyzed. Since the analysis of unidentified substances can be extremely dangerous, the methodology for analyzing the environmental pollutants among illegally disposed wastes has been established. It is especially structured with the emphasis on how to apply LC/MS.
Keywords:不法投棄

Articles
“Plasma-structural coloring” of penciling on a paper
ACS Applied materials & interfaces,15(3):4781-4788 2023
Author:Hiroshi Moriwaki, Tomoya Kamine
Abstract:We have developed a method of coloring pencil-written parts on paper in various colors by plasma irradiation. The pencil layer on the paper must be thick to form the various structural colors. Therefore, it was necessary to use a pencil containing high graphite component and to be sufficiently painted pencil lead component on the paper sample.
Keywords:構造色・鉛筆・プラズマエッチング・紙

Interaction between nanoplastics and pectin, a water-soluble polysaccharide, in the presence of Fe(III) ion
Journal of Environmental Chemical Engineering,:108054 2022
Author:Hiroshi Moriwaki, Naoya Komori, Yoshitake Akiyama
Abstract:Interaction between polystyrene nanoparticles and pectin has been studied. Flocculation of nanoplastics was occurred by the addition of pectin and Fe(III). This result contributes to the understanding of the behavior of nanoplastics in the water environment and the development of the method to remove nanoplastic from water.
Keywords:ナノプラスチック・凝集沈殿・ペクチン・バイオポリマー

Structural color on pencil lead formed by plasma etching
Advanced Optical Materials,:2102127 2022
Author:Hiroshi Moriwaki, Tomoya Kamine, Yukari Kawabe, Yusuke Okada
Abstract:A novel simple method to form the structural color on a pencil lead by the plasma irradiation for 40-120 seconds has been developed. The structural color was reproducibly formed by the plasma irradiation to the surface of a pencil lead. The color changed with the plasma irradiation time. The formed colors exhibited a luster and turned blue, yellow, red-purple, green, or pale red by the plasma irradiation time of 40, 60, 80, 100, and 120 sec, respectively. The mechanism to form the structural color was discussed.
Keywords:構造色・鉛筆・プラズマエッチング

Effect of visible light irradiation on catalytic activity of gold nanoparticles and perylene adsorbed on silica gel
Catalysis Communication,145:106116 2020
Author:Hiroshi Moriwaki, Mahiro Oshima
Abstract:A novel material (Au/Pe/SiO2: gold naoparticles and perylnee adsorbed on silica gel) was developed, and the effect of visible light irradiation on the catalytic activity of gold nanoparticles was studied. The irradiation resulted in the acceleration of the decompositions of nitrophenols and congo red by the catalytic reaction of gold nanoparticles in the presence of NaBH4.
Keywords:可視光応答型光触媒・金ナノ粒子・ペリレン・触媒反応

Simple method for removal of pollutants from water using freeze-thaw treatment
Separation and purificatiobn technology,237:116382 2020
Author:Hiroshi Moriwaki, Suzu Fujii, Mahiro Oshima
Abstract:Simple method to remove environmental pollutants from water using freeze-thaw method has been developed. The method needs only a syringe, elastic band and cotton wool. The solution (20 mL) containing an environmental pollutant was added to a syringe. The part around the outlet of the syringe was covered with cotton wool in order to control the place of the pollutants in the frozen solution. The syringe was frozen by a freezer. The frozen sample was thawed. The pollutant was concentrated in the initial fraction (1 mL) of the thawing solution. The rest was fully thawed and frozen. By repeating the treatment 3 times, the pollutant was removed over 80% from the solution.
Keywords:水浄化・凍結解凍・フッ素・ペンタクロロフェノール・鉛

Acoustic focusing of microplastics in microchannels: A promising continuous collection approach
Sensors and Actuators B: Chemical,304:127328 2020
Author:Yoshitake Akiyama, Takatoshi Egawa, Kiyoshi Koyano, Hiroshi Moriwaki
Abstract:Microplastics (microparticles and small fibers) in water were collected by acoustic focusing. The method would be useful for the removal of microplastics from aqueous suspentions, such as the effluent from landry machines.
Keywords:マイクロプラスティック・アコースティックフォーカシング

Two-step extraction method for lead isotope fractionation to reveal anthoropogenic lead pollution
Environmental Technology,40(26):3473-3478 2019
Author:Kenshi Katahira, Hiroshi Moriwaki, Kazuo Kamura, Hideo Yamazaki
Abstract:The lead isotope ratio is very important indicator to estimate the source of Pb pollution. We have developed the novel method to estimate the anthropogenic lead pollution in sediment samples by analyzing lead isotope ratio by two-step extraction.
Keywords:底質・鉛同位体比・分析

Glowing gold nanoparticle coating: restoring the lost property from bulk gold
Nanoscale,11:3786-3793 2019
Author:Yukari Kawabe, Takashi Ito, Hiroaki Yoshida, Hiroshi Moriwaki
Abstract:Gold nanoparticles (AuNP) usually show a red or blue color because of the localized surface plasmon resonance. We found that the AuNPs on the pecil graphite revealed golden color. The phenomenon would be caused by the interaction between the free electron of AuNPs and pencil graphite. The golden-colored AuNPs maintain a catalytic activity for the reduction of nitrocompounds in the presence of sodium borohydride.
Keywords:金ナノ粒子・表面プラズモン共鳴・金色・鉛筆・グラファイト

Removal of polycyclic hydrocarbon from soil using a composite material containing iron and activated carbon in the freeze-dried calcium alginate matrix: Novel soil cleanup technique
Journal of Hazardous Materials,351:232-239 2018
Author:Mako Funada, Takeshi Nakano, Hiroshi Moriwaki
Abstract:A novel clean-up technoology for the removal of polycyclic aromatic hydrocarbons in soil by magnetic separation using a composite containing iron powder as a magnetic material and activated carbon as an adsorbent in the freeze-dried calcium alginate matrix (Fe-AC-alg) has been developed.
Keywords:土壌汚染・複合材料・多環芳香族化合物・除去技術

Application of porous TiC ceramic powder as a substrate for the surface-assisted laser desorption/ionization mass spectrometry to detection environmental pollutants
International Journal of Mass Spectrometry,428:49-54 2018
Author:Hiroshi Moriwaki, Takumi Otsuka, Yukari Kawabe, Issey Osaka, Akio Miyazato, Junpei Maruo, Osamu Yamada
Abstract:Porous TiC ceramic powder was used as a substrate for the SALDI/MS method for the detection of environmental pollutants (<500 Da). PFOS, pentachlorophenol, 2,4,5-T, imidacloprid, symetryn, benzyldimethyldocecylammonium chloride, N,N-dimethyldodecylamine-N-oxide, sodium dodecyl sulfate, methyl orange, methyl red, triclosan, pyrene, and benzo(a)pyrene were detected by this method.
Keywords:SALDI/MS・環境汚染物質・多孔質TiCセラミックス

Evaluation of the interaction between pesticides and a cell membrane model by surface plasmon resonance spectroscopy analysis
Journal of Agricultural and Food Chemistry,65(26):5390-5396 2017
Author:Hiroshi Moriwaki, Kotaro Yamada, Hiromitsu Nakanishi
Abstract:Interaction between a pesticide and the cell membrane was evaluated by SPR analysis. The pattern of the interaction of the 17 pesticides with the cell membrane model were visualized. The pesticides, showed a strong interaction with the lipid bilayer, reveal toxicities for cell membranes. In addition, the method reveal a correlation between the residual ratios and the no observable adverse effect level (NOAEL) values for chronic toxicity (1 year toxicity study in dogs).
Keywords:SPR・農薬・リポソーム・相互作用・慢性毒性

Electrochemical extraction of gold from wastes as nanoparticles stabilized by phospholipid
Waste Management,60:591-595 2017
Author:Hiroshi Moriwaki, Kotaro Yamada, Hisanao Usami
Abstract:Novel method to extract gold as nano-particulate from wastes is demonstrated. The extraction of gold from wastes is achieved by applying AC voltage (100 V) for 5 seconds to the gold-containing wastes in a HEPES buffer solution containing phospholipid (DOPC) and sodium chloride.
Keywords:金・抽出・電子廃棄物・ナノ粒子

Photodegradation of environmental pollutants using perylene adsorbed on silica gel as a visible-light photocatalyst
Applied Catalysis B：Environmental,204:456-464 2017
Author:Hiroshi Moriwaki, Yasutaka Akaishi, Michiya Akamine, Hisanao Usami
Abstract:Visible light induced electron transfer reaction of Pe/SiO2 (perylene adsorbed on silica gel) was studied. Irradiation by visible light to aqueous solution containing environmental pollutants, such as methyl orange, pentachlorophenol, and imidacloprid, resulted in the decomposition of these compounds. The continuous flow system of the reaction using a clolumn packed Pe/SiO2 powder was studied. The methyl orange aqueous solution in the presence of ascorbic acid was flowed in the column, and the column was irradiated by sunlight or visible light. The continuous decomposition of methyl orange was achieved by the system.)
Keywords:ペリレン・光触媒・可視光・メチルオレンジ・ペンタクロロフェノール

Application of freeze-dried powders of Genetically engineered microbial strains as adsorbents for rare earth metal ions
ACS Applied materials & interfaces,8(40):26524-26531 2016
Author:Hiroshi Moriwaki, Reiko Masuda, Yuki Yamazaki, Kaoru Horiuchi, Mari Miyashita, Jun Kasahara, Tatsuhito Tanaka, Hiroki Yamamoto
Abstract:The adsorption behaviors of the rare earth metal ions onto freeze-dried powders of wild type and genetically engineered microbial strains, Bacillus subtilis 168 wild type (WT), lipoteichoic acid defective (ΔLTA), wall teichoic acid-defective (ΔWTA), and cell wall hydrolases-defective (EFKYOJLp), were compared. The distribution coeﬃcients between the kinds of lanthanide ions by the powders were different. The results indicate that these powders could be applicable for the selective extraction of the lanthanide ions.
Keywords:枯草菌・遺伝子操作・希土類・選択性・抽出

Electrospray ionization mass spectrometric detection of low polar compounds by adding NaAuCl4
Journal of Mass Spectrometry,51:1096-1102 2016
Author:Hiroshi Moriwaki
Abstract:LC/ESI/MS is very useful technique for trace analysis. However, the technique is not suitable for the analysis of low-polar compounds. In this report, a novel method for the ESI/MS detection of low-polar compounds by adding NaAuCl4 has been developed.
Keywords:エレクトロスプレー　質量分析　多環芳香族化合物　塩化金酸

Decomposition of methyl orange using C60 fullerene adsorbed on silica gel as a photocatalyst via visible-light induced electron transfer
Applied Catalysis B：Environmental,166:544-550 2015
Author:Ryo Wakimoto, Tamako Kitamura, Fuyu Ito, Hisanao Usami, Hiroshi Moriwaki
Abstract:Visible light induced electron transfer reaction of C60/SiO2 (C60 adsorbed on silica gel) was studied. Irradiation by visible light to methyl orange aqueous solution with ascorbic acid resulted in the decomposition of methyl orange. The reaction was very efficeintly. The continuous flow system of the reaction using a clolumn packed C60/SiO2 powder was studied. The methyl orange aqueous solution in the presence of ascorbic acid was flowed in the column, and the column was irradiated by sunlight or visible light. The continuous decomposition of methyl orange was achieved by the system.)
Keywords:フラーレン・光誘起電子移動反応・メチルオレンジ・可視光応答型触媒


Application of fullerenes-extracted soot modified with ethylenediamine as a novel adsorbent of hexavalent chromium in water
Journal of Environmental Chemical Engineering,2(2):1191-1198 2014
Author:Takahiro Nakagawa, Ken Kokubo, Hiroshi Moriwaki
Abstract:This is the first report that fullerenes-extracted soot, the by-product of fullerene production, was used to purify water. Fullerenes-extracted soot modified with ethylenediamine (FES-ED) shows high adsorption activity for Cr(VI). The maximum adsorption capacity of FES-ED for Cr(VI) was 93.5 mg/g. The adsorbent showed good reusability.
Keywords:フラーレンスート・六価クロム・除去・水浄化


Development of a novel evaluation method for air particles using surface plasmon resonance spectroscopy analysis
Analyst,138(18):5437-5443 2013
Author:Ryoya Tanaka, Ryusaku Gomi, Kunihiro Funasaka, Daichi Asakawa, Hiromitsu Nakanishi, Hiroshi Moriwaki
Abstract:This study develops a novel method to visualize the interaction between particulate matters and the liposome using a surface plasmon resonance spectroscope with a sensor-chip immobilized liposome as a model of the membrane of epithelial cells in the organ of respiration. Results obtained by the presented method are useful for not only the analysis of the risk assessment of aspirated particulates in the body, but also for estimation of the generation source of air pollution.
Keywords:表面プラズモン共鳴分析・大気微粒子・PM2.5・新規評価法


Removal of Pb(II) from water using keratin colloidal solution obtained from wool
Environ. Sci. Pollut. Res.,20(9):6531-6538 2013
Author:Yuri Sekimoto, Tomoki Okiharu, Haruka Nakajima, Toshihiro Fujii, Koji Shirai, Hiroshi Moriwaki
Abstract:Keratin colloidal solution extacted from wool has been used for the removal of Pb(II). The addition of keratin colloidal solution to a Pb(II) solution resulted in the formation and sedimentation of keratin-Pb aggregate. This method contributes to the water purification and the reuse of waste materials.
Keywords:羊毛・ケラチン・鉛


Nishiyama reservoir: Lead sources, inventory, and the influence of the Nagasaki atomic bomb
Soil Sediment Contam.,22:1003-1012 2013
Author:Kenshi Katahira, Hiroshi Moriwaki, Miho Ishitake, Yoko Saito-Kokubu, Hideo Yamazaki, Shusaku Yoshikawa
Abstract:The sediment core was obtained from Nishiyama Reservoir, 3 km east from the hypocenter of Nagasaki atomic bomb. The historical trends of heavy metal ion in the core samples were analyzed, and spiked peaks of Pu and Pb were observed at around 1945. It has been suggested that the peak of Pb would due to the influence of the atomic bomb attack by the analysis of Pb isotopic ratios.
Keywords:原爆・プルトニウム・底質コア試料・鉛同位体比

Adsorption of rare earth ions onto the cell walls of wild type and lipoteichoic acid-defective strains of Bacillus subtilis
Appl. Microbiol. Biotech.,97(8):3721-3728 2013
Author:Hiroshi Moriwaki, Remi Koide, Ritsuko Yoshikawa, Yuya Warabino, Hiroki Yamamoto
Abstract:The adsorption of rare earth metal ions onto cell walls of wild-type (WT) and lipoteichoic acid-defective (ΔLTA) strains of Bacillus subtilis 168 were studied. Removal (in percent) of Tm(III), La(III), and Eu(III) from aqueous solution by WT powder was greater than by ΔLTA powder strains of Bacillus subtilis 168. Furthermore, the ΔLTA powder readily coagulated and sedimented in the presence of rare earth ions, though such coagulation and sedimentation did not occur with WT powder. From these facts, it is concluded that the bioengineered microorganism, ΔLTA powder, can be applied to a novel useful adsorbent of rare earth ions.
Keywords:枯草菌・レアアース・吸着・遺伝子操作株・リポテイコ酸


Interactions of microorganisms with rare earth ions and their utilization for separation and environmental technology
Appl. Microbiol. Biotech.,97(1):1-8 2013
Author:Hiroshi Moriwaki, Hiroki Yamamoto
Abstract:The application and mechanisms of microorganisms for the removal and extraction of rare earth elements from aqueous solutions are described.
Keywords:枯草菌・レアアース・吸着


Electrospray ionization mass spectrometric observation of the interaction between cesium ions and amino acids
Rapid Communication in Mass Spectrometry,26:2822-2826 2012
Author:H. Moriwaki, T. Nakagawa, H. Nakanishi
Abstract:Large quantity of radioactive cesium species were emitted to environment by disaster at Fukushima Daiichi Nuclear Power Plant. It is thought that people who lived around the accident site were exposed radioactive cesium, and the possibility that they exhibit the adverse effects of the exposure is of particular concern. In this research, aqueous solutions containing cesium ion and amino acids were analyzed by ESI/MS with the aim of gaining information on the interaction of cesium ion with amino acids or peptides. Acidic amino acid, in particular, showed a high affinity with cesium ion.
Keywords:放射性セシウム・アミノ酸・相互作用・エレクトロスプレーイオン化法

大阪市域土壌における自然由来の鉛の同位体比
地学雑誌,120(4):599-614 2011(Sep.)
Author:加田平賢史, 森脇　洋, 吉川周作, 新矢将尚, 北野雅昭
Abstract:We examinedPb isotope ratios in boring cores taken from sediments in the Osaka City area, and found that the isotople ratio of lead could be used to determine whether lead was anthoropogenic or natural.
Keywords:酸溶解成分・鉛同位体比・ボーリングコア

Reaction of congo red in water by irradiating pulsed intense relativistic electron beam
Plasma and Fusion Research,6:1206021 2011(May)
Author:Takashi Kikuchi, Hiroshi Moriwaki, Hiromitsu Nakanishi, Hironobu Kondo, Toru Sasaki, Go Imada, Nob. Harada
Abstract:Congo red, a well-known toxic azo dye, was reacted by irradiation of a pulsed intense relativistic electron beam. The products were the hydroxylated compounds of the dye.
Keywords:染料・分解・電子ビーム

Application of the powder of porous titanium carbide ceramics to a reusable adsorbent for environmental pollutants
Journal of Hazardous Materials,185(2-3):725-731 2011(Feb.)
Author:Hiroshi Moriwaki, Shiori Kitajima, Koji Shirai, Kenji Kiguchi, Osamu Yamada
Abstract:TiC porous ceramics is a novel material prepared by Prof. Yamada at Osaka Sangyo University. We studied the application of the material on the environmental clean-up technology. The powder of TiC ceramics could use for the adsorbent for pollutants in water and the sorbent for solid-phase extraction in environmental analysis.
Keywords:多孔質吸着体・TiC・セラミックス・鉛・PFOS・PFOA

旧硫黄鉱山による汚染が河川環境に与える影響－百々川水系の水質ならびに微生物の生息状況－
生活衛生,54(4):321-329 2010(Oct.)
Author:伊藤吹夕, 野村隆臣,加田平賢史, 北川幹也, 森脇　洋
Abstract:The water of the Dodo river is acidic because of the influence of abandoned sulfur mines. The water quarity and microbial habitats in Dodo river system were investigated. The river water showed high concentration of sulfate ion (46 ppm). In addition, using the genetic PCR-DGGE method, it was confirmed that there were many microorganisms living.
Keywords:百々川・旧硫黄鉱山・バクテリア

Effect of deuterated solvents toward 2,2,2-trichloroethyl esters with a benzylic methylene moiety
Tetrahedron Letter,51:6045-6048 2010(Oct.)
Author:Tomoko Mineno, Haruyasu Hirayama,　Kazuhide Nakahara, Mitsuaki Yamashita, Hisao Kansui, Hiroshi Moriwaki
Abstract: the 2,2,2-trichloroethyl moiety has beenused as a convenient protecting unit. The indium-promoted chemoselective deprotection of 2,2,2-trichloroethyl esters containing a benzylicmethylene was successfully achieved by employing deuterated solvents.
Keywords:インジウム・脱保護基・2,2,2-トリクロロエチルエステル

Electrospray ionization-mass spectrometric measurement of sake, the Japanese traditional alcohol beverage, for characterization
Analytical Sciences,26(3):379-382 2010(Mar.)
Author:Hiroshi Moriwaki, Ayaka Hagiwara, Midori Takasaki, Fuminori Izumi, Arata Watanabe, Ryo Shimizu, Natsuko Kuribayashi, Yui Totani, Yuka Suzuki
Abstract:Electrospray ionization-mass spectrometric measurement of sake and principle analysis of peak intensities of ions corresponding to the contents have been applied to the characterization of sake.
Keywords:日本酒・エレクトロスプレーイオン化法・キャラクタリゼーション


新しくPOPs登録された化学物質のLC/MSによる分析
環境浄化技術,8(11):43-47 2009(Nov.)
Author:森脇　洋
Abstract:The LC/MS techniques for the determination of POPs additionally resisterred in 2009 were discussed.
Keywords:POPｓ・LC/MS

Simultaneous LC/MS Analysis of Hexachlorobenzene and　Pentachlorophenol by Atmospheric Pressure Chemical Ionization (APCI)　and Photoionization (APPI) Methods
Analytical Sciences,25(11):1373-1376 2009(Nov.)
Author:Issey Osaka, Arifumi Yoshimoto, Kazuyoshi Nozaki, Hiroshi Moriwaki, Hideya Kawasaki, Ryuichi Arakawa
Abstract:Since the polarities of hexachlorobenzene (registered as POPs) and pentachlorophenol differ largely, it has been difficult to conduct mass analysis without derivatization. This research has indicated that it is possible to analyze these compounds simultaneously, byusing LC/APCI/MS, or LC/APPI/MS. Especially when using APPI/MS, highly-sensitive analysis was possible.
Keywords:ヘキサクロロベンゼン・ペンタクロロフェノール・LC/MS

Statistical analysis of metal concentrations in a sediment core to reveal influences of human activities on atmospheric environment for 200 years
Water, Air,&Soil Pollution,204:215-225 2009(Nov.)
Author:Kenshi Katahira, Miho Ishitake, Hiroshi Moriwaki, Osamu Yamamoto, Tadao Fujita, Hideo Yamazaki, Shusaku Yoshikawa
Abstract:The bottom sediment core sample, which consisted of atmospheric fallouts of 200 years, was collected from the moat of Osaka Castle. By dating, measuring the concentration of heavy metals depth by depth, and carrying out analysis statistically, the historical transitions of heavy metal contamination into the atmosphere, at the central urban city, for the last 200 years were successfully comprehended.
Keywords:環境分析・大気汚染

納豆を用いた凝集沈殿の実験―環境教育における実験への生物資源の応用―
生活衛生,53(4):271-274 2009(Oct.)
Author:森脇　洋, 矢口　祐, 原口和真, 赤嶺総哉, 鵜之沢英理, 北原恵里子, 松尾京子, 松島祐達
Abstract:Flocculation using natto, a traditional Japanese food, as flocculant was investigated. We found that natto can be useful for a flocculant and experimental material for school environmental education.
Keywords:納豆・凝集沈殿・環境教育

Electrospray ionization mass spectrometric observation of ligand exchange of zinc pyrithione with amino acids
Rapid Commun. Mass Spectrom,23:2161-2166 2009(Jul.)
Author:Hiroshi Moriwaki, Masanori Okabayashi, Takehiro Watanabe, Hideya Kawasaki, Ryuichi Arakawa
Abstract:The antibacterial agent, zinc pyrithione is widely used in shampoos and antifoulant paints. Recently, it has been reported, that the zinc pyrithione causes teratogenesis in fish, however the details of such mechanism has not been revealed. Therefore, an investigation to examine the dynamic state of zinc pyrithione in organisms was carried out, by using electrospray ionization/mass spectrometry, so that how zinc pyrithione reacts with different kinds of amino acids could be found out. As a result, it has become apparent, that zinc pyrithione easily reacts with histidine.
Keywords:ジンクピリチオン・毒性


大阪市域のボーリングコア試料を用いた自然起源の鉛・ヒ素の分布特性
地質汚染－医療地質－社会地質学会誌,5:11-24 2009(Jun.)
Author:加田平賢史・森脇洋・吉川周作・七山太・山本攻・藤田忠雄
Abstract:Osaka plains possess the geological stratum which is called the marine clay, containing relatively high concentration of elements, such as arsenic and iron. Also, since the marine clay contains a lot of sulfide, when it is exposed to the sun, its eluate becomes acidic. This leads to a possibility, that once the marine clay gets exposed by underground constructions, exceeding amount of arsenic and lead could be found during an elution test, in comparison to the environmental standard. In this research, by using the boring core sample from Osaka city area, elution behavior of arsenic and lead within the marine clay was observed, and correlation between the marine clay and soil contamination was examined.
Keywords:海成粘土層・ヒ素

Utilization of silkworm cocoon waste as a sorbentfor the removal of oil from water
Journal of Hazardous Materials,165:266-270 2009(Jun.)
Author:Hiroshi Moriwaki, Shiori Kitajima, Masahiro Kurashima, Ayaka Hagiwara, Kazuma Haraguchi, Koji Shirai, Rensuke Kanekatsu and Kenji Kiguchi;
Abstract:The damaged silkworm cocoons are generated by the sericulture industry. The possible utilization of such damaged silkworm cocoons as oil sorbent material was investigated. It was discovered that 1g of flocculate material made from the damaged silkworm cocoons absorbs about 40g of vegetable and motor oils. Thus, it has been made evident that it possesses high oil-sorption capacity compared to other types of oil sorbent materials. It has been deduced, that such utilization of damaged silkworm cocoons is meaningful, because it reuses industrial waste and enviromentally friendly material for the environmental purification.
Keywords:くず繭・油除去


大阪平野沖積層の自然由来の重金属等の溶出挙動－ボーリングコア試料を用いた検討－
地学雑誌,118(2):261-279 2009(May)
Author:加田平賢史・森脇　洋・吉川周作・七山　太・山本　攻
Abstract:The core samples of soil collected at Osaka Plains were analized, for heavy metals and ions, in order to examine them compared to the environmental quality standard for soil. In the layer under no artificial influences, fluorine which exceeded the regulation was detected. In addition, acid soluble elements were also measured, and the elution behaviors of various elements were described.
Keywords:地質・環境科学

Analysis of chlorothalonil by liquid chromatography/mass spectrometry using negative atmospheric pressure photoionization
Analytical Sciences,25:693-697 2009(May)
Author:Atsushi Yamamoto, Iori Miyamoto, Mikiya Kitagawa, Hiroshi Moriwaki, Hidekazu Miyakoda, Hideya Kawasaki, Ryuichi Arakawa
Abstract:Conventionally, chlorothalonil, which is commonly used as a pestiside, has been a difficult object of sensitive analysis by using the mass spectrometry. Therefore, liquid chromatography/tandem mass spectrometry using the photoionization has been applied to an analysis of chlorothalonil and its metabolites. By this method, the trace analysis of these chemical compounds has become possible. Application of this method in the environmental analyses and in the food analyses have been indicated.
Keywords:環境分析・LC/MS

Waste on the roadside, 'poi-sute' waste: Its distribution and elution potential of pollutants into environment
Waste Management,29:1192-1197 2009(Mar.)
Author:Hiroshi Moriwaki, Shiori Kitajima, Kenshi Katahira
Abstract:The littered trash on the roadside called 'Poi - Sute' garbage around the campus of the Faculty of Textile Science, Shinshu University, were collected every month for the purpose of sampling, in order to check their compositions, distributions, and amounts. Furthermore, an amount of environmental pollutants generated by these littered trash were estimated. When studying the compositions of such garbages, the largest number was exhibited by cigarette butts. It is also indicated, that harmful substances such as arsenic and polycyclic aromatic hydrocarbons are eluted from littered cigarette butts possibly, into the environment.
Keywords:ポイ捨てごみ


Layer-by-Layer Self-Assembled Muｌtilayer Films of Gold Nanoparticles for Surface-Assisted Laser Desorption/Ionization Mass Spectrometry
Analytical Chemistry,80(19):7524-7533 2008(Oct.)
Author:Hideya Kawasaki, Tsuyoshi Sugitani, Takehiro Watanabe, Tetsu Yonezawa, Hiroshi Moriwaki, Ryuichi Arakawa;
Abstract: It has been revealed, that when using multilayer film of gold nanoparticles as a matrix for the surface-assisted laser desorption/ionization, highly-sensitive measurements of peptide and environmental pollutants (pyrene, etc.) by SALDI/TOF/MS　are possible.
Keywords:分析法・質量分析


Detection of Pb-LIII edge XANES spectra of urban atmospheric particles combined with simple acid extraction
Science of the Total Environment,403:230-234 2008(Aug.)
Author:Kunihiro Funasaka, Toshiki Tojo, Kenshi. Katahira, Masashi Shinya, Takeji Miyazaki, Toshikazu Kamiura, Osamu Yamamoto, Hiroshi. Moriwaki, H. Tanida, M. Takaoka
Abstract:It is extremely important to know the configuration of hazardous metals existing in the environment, in order to know the origins of such metals and to discuss their toxicities or risks in the environment. By observing the different extraction methods and XANES spectra of led, which are found in the atmospheric particles, the new method to discuss its configuration has been suggested in this article.
Keywords:環境分析・形態


Effect of mixing metal ions on DNA oxidative stress via Fenton-type reactions
Toxicology in Vitro,22:36-44 2008(Jan.)
Author:Hiroshi Moriwaki, Martin R. Osborn, David H. Phillips
Abstract:The combined effect of hazardous metals against DNA has been investigated. After mixing each of Fe, Cu, Cd, Cr, and Ni with DNA, hydrogen peroxide has been added in order to generate activated oxygen by Fenton-type reactions, so that the DNA stress can be measured. Next, 2 elements out of these 5 elements were chosen and mixed with DNA simultaneously, in order to measure the DNA stress after adding hydrogen peroxide. Afterward, the summations of DNA oxidative stress in both cases were compared. Cd and Ni had inhibited DNA oxidative stress caused by other metal ions. On the other hand, the synergistic effect was observed in the mixed Cu and Cr. Although the discussion is based on "in vitro", it has been revealed, that DNA oxidative stress induced by hazardous metals receives combined effect due to the interaction between hazardous metals and DNA.
Keywords:毒性・複合影響


重金属濃度と鉛同位対比からみた長崎湾底質コアにおける環境変遷史
第18回環境地質シンポジウム論文集,18:151-154 2008
Author:高坂由依子、加田平賢史、森脇　洋、山崎秀夫、國分（齋藤）陽子、吉川周作

Vertical profile of polycyclic aromatic hydrocarbons in a sediment core from a reservoir in Osaka City
Environment Geology,52:123-129 2007(Mar.)
Author:Miho Ishitake, Hiroshi Moriwaki, Kenshi Katahira, Osamu Yamamoto, Kenshiro Tsuruho, Hideo Yamazaki, Shusaku Yoshikawa
Abstract:After collecting the bottom sediment core sample from a reservoir in Osaka City and performing the dating, the concentration of polycyclic aromatic hydrocarbons for each sediment layer was measured. Since there was hardly any inflow or outflow of water into a reservoir, it was considered that its sediment mainly consisted of airborne deposits, and that sediment core sample had recorded the historical changes in the atmospheric pollution. As a result of the analysis, the highest concentration of polycyclic aromatic hydrocarbons was recorded during World War II. It was estimated that this was due to the air raid of Osaka City by the US forces.
Keywords:環境分析・大気汚染


Analysis of p,p’-DDT by liquid chromatography-atmospheric pressure photoionization/ mass spectrometry
Rapid Communication in Mass Spectrometry,21:1090-1092 2007(Mar.)
Author:Hiroshi Moriwaki
Abstract:The p,p’-DDT is known as one of the persistent organic pollutants (POPs), and is a significant target within the environmental analysis. However, no measurement example by LC/MS had been reported. This article addresses the detection method of p,p’-DDT by LC/MS. It has been confirmed, that by using APPI method, p,p’-DDT can be detected. Furthermore,by connecting LC and constructing a calibration curve, the concentration to ppb level can be measured by LC/MS.
Keywords:環境分析・LC/MS

Method for the estimation of the past illegal dumping recorded in a sediment core
Water, Air,&Soil Pollution,179:197-206 2007(Feb.)
Author:Kenshi Katahira, Miho Ishitake, Hiroshi Moriwaki, Syusaku Yoshikawa, Osamu Yamamoto, Tadao Fujita, Hideo Yamazaki
Abstract:After collecting the bottom sediment core sample from a reservoir in Osaka City and performing the dating, the concentration of heavy metals for each sediment layer was measured. Only in the heavy metal cases such as Cd, Ni, and Cr, that were used in metal platingindustry, a sudden elevation in concentration was observed around 1960s. After the statistical analyzation, it was deduced, that this sharp rise of concentration in 1960s was caused by the illegal dumpings of liquid waste from the metal plating factories. It was extremely rare for sediment core sample to be recording such environmental event.
Keywords:環境分析・重金属


Liquid chromatography-mass spectrometry for the analysis of environmental mutagens
Current Anal. Chem.,3:69-79 2007(Jan.)
Author:Hiroshi Moriwaki
Abstract:The environmental mutagens possess the danger to operate in such a way, which brings harmful influence to living creatures. Therefore, an establishment of analytical method regarding these substances in the environment is extremely important. Since there are many chemical substances within mutagens, that are highly polar and are not fit for an analysis by GC, the analytical method to use LC/MS has been actively pursued recently. Within this article, the environmental mutagens have been detected by LC/MS, and their measurement examples have been summarized. It has also revealed, that the application of LC/MS towards the environmental analysis is important, and that such application is highly effective.
Keywords:総説・環境分析

液体クロマトグラフィー質量分析による残留性有機汚染物質 (POPs)類の分析
日本質量分析科学会誌,55:183-191 2007
Author:森脇　洋

パーフルオロオクタンスルホン酸(PFOS)とパーフルオロオクタン酸(PFOA)の超音波による分解
超音波テクノ,19:30-33 2007
Author:森脇　洋

自然由来の土壌中重金属の含有量と溶出量
環境浄化技術,6:31-36 2007
Author:加田平賢史、森脇洋、山本攻、吉川周作

Electrospray ionization-mass spectrometric detection of hexachlorocyclohexane
Rapid Communication in Mass Spectrometry,20:1963-1964 2006(Jun.)
Author:Hiroshi Moriwaki
Abstract:The hexachlorocyclohexane is known as one of the persistent environmental pollutants (POPs) and is a significant target within the environmental analysis. However, there had been no report of measurement examples by LC/MS. This article addresses the detection method of hexachlorocyclohexane by LC/MS. Hexachlorocyclohexane could be detected by using ESI/MS. As for the mechanism of ionization, the possibility of SN1 reaction occuring within the ESI interface to produce ions was indicated.
Keywords:環境分析・大気汚染


不法投棄廃棄物分析のための方法論と技術
環境浄化技術,5:48-52 2006
Author:森脇　洋

Liquid chromatographic- mass spectrometric methods for the analysis of persistent pollutants: polycyclic aromatic hydrocarbons, organochlorine compounds and perfluorinated compounds
Current Organic Chemistry,9:849-857 2005(Sep.)
Author:Hiroshi Moriwaki
Abstract:The analysis of persistent pollutants is of utter importance. However, it is difficult to analyze many chlorine-based persistent pollutants, if using LC/MS, which has been popularly used recently. This article describes the detection method of polycyclicaromatic hydrocarbons, that are considered to be difficult to detect by LC/MS, and of lowly polarcompounds such as organochlorine pesticides. The detection of these compounds by LC/MS have been summarized so that the importance of such method and its future development can be recounted.
Keywords:総説・環境分析

Determination of phenolic metabolites in urine of rats treated with 1,2,3- and 1,3,5- trimethylbenzenes
J. Occup. Health,47:337-339 2005(Aug.)
Author:Yuji Tsujimoto, Munehiro Warashina, Tsutomu Noda, Mitsuru Shimizu, Yukihiko Yamaguchi, Hiroshi Moriwaki, Masanobu Tanaka
Abstract:The trimethylbenzenes are widely used as organic solvents and exist extensively in the indoor environment. In order to know the metabolism of 1,2,3-trimethylbenzenes and 1,3,5-trimethylbenzes, these substances were administered to rats and metabolites within their urine were measured. In general, methylbenzenes turn into safe hippuric acid during a metabolism process. However, it has been discovered, that trimethylbenzenes have high tendency to take part in a phenolic metabolism, which has high toxicity.
Keywords:尿中代謝物・LC/MS

Influence of sodium benzoate on the metabolism of o-xylene
Xenobiotica,35:487-497 2005(May)
Author:Hiroshi Moriwaki, Yuji Tsujimoto, Mitsuru Shimizu, Tsutomu Noda, Munehiro Warashina, Masanobu Tanaka
Abstract:The sodium benzoate has been used extensively as a food additive. It is known to be safely metabolized and be discharged from urine as hippuric acid. On the other hand, the indoor pollutant methylbenzenes turn themselves into benzoates during the process of metabolism. Thus, we had exposed both sodium benzoate and methylbenzene to rats and observed what would happen to their metabolism. As a result, it was revealed that the metabolism of methylbenzene was inhibited.
Keywords:毒性・代謝


Sonochemical decomposition of perfluorooctane sulfonate and perfluorooctanoic acid
Environment Science&Technology,39:3388-3392 2005(May)
Author:Hiroshi Moriwaki, Youichi Takagi , Masanobu Tanaka, Kenshiro Tsuruho, K. Okitsu, Y. Maeda
Abstract:The decomposition method of PFOS and PFOA by sonication has been developed. PFOS and PFOA are persistent substances, and the establishment of their decomposition method has been much desired. Within this article, the sonication has been irradiated to the PFOS and PFOA solution, and by using measurements by LC/MS, its mechanism has been observed. As a result, more than 50% of PFOS and PFOA had resolved after an hour of sonication irradiation.
Keywords:分解反応・環境科学


Historical trends of polycyclic aromatic hydrocarbons in the reservoir sediment core at Osaka
Atmospheric Environment,39(6):1019-1025 2005(Feb.)
Author:Hiroshi Moriwaki, Kenshi Katahira, Osamu Yamamoto, Joji Fukuyama, Toshikazu Kamiura, Hideo Yamazaki, Shusaku Yoshikawa
Abstract:After collecting the bottom sediment from Osaka Castle, the dating was performed per each depth. When the polycyclic aromatic hydrocarbons within the sample was analyzed, it was ascertained, that the greatest amount of polycyclic aromatic hydrocarbons were recorded during the war. It was considered, that this result was due to the influence inflicted by air raids to military arsenals, which were located around Osaka Castle. Within the 350 years of history in Osaka, it has become evident, that it was World War II, which had caused the most impact to the environment.
Keywords:環境分析・大気汚染


Simultaneous determination of 2-phenylbenzotriazole-type mutagens, PBTA-1~8, in river water by liquid chromatography-tandem mass spectrometry
Journal of Environmental Monitroing,6:897-902 2004(Nov.)
Author:Hiroshi Moriwaki,　Hiroya Harino, Taro Yoshikura, Takeshi Ohe , Haruo Nukaya, Yoshiyasu Terao, Hiroyuki Sawanishi, Keiji Wakabayashi, Hidekazu Miyakoda, Jean-Francois ALARY
Abstract:Regarding the high mutagenicity of water in Yodo river district, 8 kinds of PBTA compounds, the PBTA1-8, have been isolated as part of the substances responsible for mutagenicity. The simultaneous highly sensitive analytical method has been developed for these 8 kinds of substances, by using LC/MS/MS. By this method, easy analysis of minimal amount in short time was possible. Several PBTAs have been detected by this method from the actual river water, proving that this method can actually be applied to the environmental analysis.
Keywords:環境分析・LC/MS

Detection of chlordecone by liquid chromatography tandem mass spectrometry
Rapid Communication in Mass Spectrometry,18:1243-1244 2004(Jun.)
Author:Hiroshi Moriwaki, Atsuko Hasegawa
Abstract:The chlordecone is widely known as a highly persistent pesticide and is an important target within the environmental analysis. However, the measurement examples of the compound by LC/MS had not been reported. This article describes the analytical method of chlordecone by LC/MS/MS. By using LC/MS/MS, highly selective and sensitive analysis was achieved. In comparison with the traditional GC/MS method, the quantitative limit of this method is nearly 100 times lower, and it has been ascertained that this method is exceptionally effective in environmental analysis of infinitesimal quantity of chlordecone.
Keywords:環境分析・LC/MS

Determination of polycyclic aromatic hydrocarbons in sediment by liquid chromatography-atmospheric pressure photo ionization-mass spectrometry
Analytical Science,20:375-377 2004(Feb.)
Author:Hiroshi Moriwaki,　Miho Ishitake, Shusaku Yoshikawa, H. Miyakoda,J-F.Alary
Abstract:Polycyclic aromatic hydrocarbons are referred to as carcinogens and are present widely in the environment as pollutants. The development of its analytical method is important, however in the past, trace analysis of polycyclic aromatic hydrocarbons by LC/MS has been extremely difficult. This article addresses the aim to analyze polycyclic aromatic hydrocarbons within the sediment by LC/APPI/MS method. As a result, the analysis of these substances within an environmental sample at the ppb level was successfully achieved. Furthermore, the acquired analytical method was actually applied to the sample sediment, displaying its availability for the environmental analysis.
Keywords:環境分析・LC/MS

Detection of technical chlordane by liquid chromatography / atmospheric pressure chemical ionization / mass spectrometry
Rapid Communication in Mass Spectrometry,18:225-227 2004(Jan.)
Author:Hiroshi Moriwaki, Masashi Yamaguchi, Hiroyuki Hashimoto and Ryuichi Arakawa
Abstract:The chlordane is known as one of the persistent organic pollutants and is a significant target in the environmental analysis. However, anexample of its measurement by LC/MS had not been reported. This article describes the detection method of chlordane by LC/MS. By using APCI, chlordane can be detected. It also indicates that by connecting LC and constructing a calibration curve, its concentration can be measured at the ppb level.
Keywords:環境分析・LC/MS

Concentrations of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) in the vacuum cleaner dust collected in Japanese homes
Journal of Environmental Monitroing,5:753-757 2003(Sep.)
Author:Hiroshi Moriwaki, Yumiko Takata, Ryuichi Arakawa
Abstract:PFOS, PFOA, that are used in water repellants are extensively dispersed in the environment and are extremely difficult to decompose. Their influence on human bodies have been of great concern. The fluorine compounds, PFOS and PFOA within dusts collected from a vacuum cleaner have been measured, and the possibility of humans being exposed to these substances through living environment has been presented.
Keywords:環境分析・LC/MS

Determination of aromatic amine mutagens, PBTA-1 and PBTA-2, in river water by solid phase extraction followed by liquid chromatography-tandem mass spectrometry
Journal of Chromatography A,995:239-243 2003(May)
Author:Hiroshi Moriwaki, Hiroya Harino, Hiroyuki Hashimoto, Ryuichi Arakawa, Takeshi Ohe, Taro Yoshikura
Abstract:The high mutagenicity of river water in Yodo river has generated the pursuit for such substances that are causing it. As a result, substances were identified as PBTAs produced from dyes, which originate from drainage at wastewater treatment plant in Kyoto. This article describes the novel analytical method of PBTA, in river water using LC/MS. The present method is very simple and the analysis of PBTAs is accomplished within a short time.
Keywords:環境分析・LC/MS

Simultaneous determination of metabolites of trimethylbenzenes, dimethylbenzylmercapturic acid and dimethylhippuricacid, in human urine by solid-phase extraction followed by liquid chromatography-tandem mass spectrometry
Journal of Mass Spectrometry,37:1152-1157 2002(Nov.)
Author:Hiroshi Moriwaki, Ayumi Watanabe, Ryuichi Arakawa, Yuji Tsujimoto, Mitsuru Shimizu, Tsutomu Noda, Munehiro Warashina, Masanobu Tanaka
Abstract:The trimethylbenzenes are widely used as solvents, and exist extensively in the indoor environment. It is important to comprehend their metabolic reactions, in order to grasp an amount and effects of these substances, when they are taken into human bodies. In this article, themethod to simultaneously analyze hippuric acid and mercapturic acid, that are urine metabolites of trimethylbenzenes has been introduced. The mercapturic acid produced from trimethylbenzenes within urine was detected successfully for the very first time.
Keywords:尿中代謝物・LC/MS

Complexes of cadmium ion with guanine bases detected by electrospray ionization-mass spectrometry
Journal of Mass Spectrometry,38:321-327 2002(Mar.)
Author:Hiroshi Moriwaki
Abstract:The cadmium is known as carcinogens. It is acutely important to gain information regarding the interaction between DNA base and cadmium, in order to consider the behavior cadmium gives to genes. Therefore, a solution mix of various DNA bases and cadmium was prepared, and by ESI/MS, its complex formation was observed. As a result, many different complexes were found regarding guanosine, such as a one which formed complex with guanosine quadroplex. However, regarding other bases, such diverse complex formation was not observed.
Keywords:毒性・LC/MS

Complexes of Paraquat with guanine bases detected by electrospray ionization-mass spectrometry
Journal of Mass Spectrometry Society in Japan,50(1):18-20 2002(Jan.)
Author:Hiroshi Moriwaki
Abstract:The interaction between paraquat, a pesticide, and guanine base was observed using ESI/MS. A mass spectrum ion peak, which corresponds with complexes formed from 1-8 guanine bases attached to 1 paraquat molecular was detected.
Keywords:超分子・LC/MS

Direct detection of Ziram by liquid chromatography / atmospheric pressure chemical ionization mass spectrometry
Rapid Communication in Mass Spectrometry,15:2374-2376 2001(Dec.)
Author:Hiroshi Moriwaki, Yukihiko Yamaguchi, Minoru Fukushima
Abstract:Ziram is an organometallic complex which consists of zinc coordinated by two dithiocarbamates, and is used as a pesticide. It possesses various toxicities, therefore it is important to grasp its concentration distribution. Organometallic complex is lowly volatile, thus it is difficult to analyze by applying GC/MS, which are widely used in the environmental analysis. The detection was attempted using LC/MS, since it allowed the detection of nonvolatile substances. By using APCI method, ziram was directly detected.
Keywords:環境分析・LC/MS

Detection of 1:1 and 2:1 complexes of nonylphenol ethoxylates with alkali metal cations by electrospray ionization mass spectrometry
Rapid Communication in Mass Spectrometry,15:2208-2210 2001(Nov.)
Author:Hiroshi Moriwaki, Takeshi Nakano, Shinji Tsunoi, Minoru Tanaka
Abstract:It has been discovered by ESI/MS, that nonylphenol ethoxylates forms complexes with alkali metals as if with crown ether. Difference in length of ethoxylate chains caused different complex formations with alkali metals. The longer the chains were, the more tendency there was to form complexes with alkali metals, which possessed larger atomic radiuses, indicating the supramolecular behavior.
Keywords:超分子・LC/MS

Direct determination of NO2-substituted pyrenes by liquid chromatography- atmospheic pressure chemical ionization- mass spectrometry
Analytical Sciences,16:1247-1248 2000(Dec.)
Author:Hiroshi Moriwaki, Kunihiro Funasaka, Michiko Uebori
Abstract:The NO2-substituted polycyclic aromatic compounds are known to reveal mutagenicity, thus their environmental analysis is of significant meaning. These substances are unstable thermally, and a measurement by GC has proved to be difficult. Although the luminescent method to perform sensitive analysis had been reported, derivatization was also required. Within this article,the direct detection method of nitro aromatic compounds such as nitropyrene, by using LC/APCI/MS has been described. By applying this method, nitropyrenes which were isolated within the environment were successfully detected.
Keywords:LC/MS

Determination of mercapturic acids in urine by solid-phase extraction followed by liquid chromatography ionization mass spectrometry
Analyst,125:715-718 2000(Apr.)
Author:Hiroshi Moriwaki, Yuji Tsujimoto, Tsutomu Noda, Mitsuru Shimizu, Masanobu Tanaka
Abstract:The mercapturic acid in urine is a metabolite of methylbenzenes, which are considered to be significant targets as indoor pollutants. Therefore mercapturic acid is an effective barometer of exposures to methylbenzenes. In this article, the analytical method to detect mercapturic acid in urine by using solid-phase extraction and LC/MS has been described. By using octadecylsilyl silica gels as solid-phase extraction, mercapturic acid was successfully extracted from urine. With this method, an easy and speedy analysis of mercapturic acid has been achieved.
Keywords:メルカプツール酸・尿中代謝物


Identification of the dimethylbenzyl mercapturic acid in urine of rats administered with 1,2,4-trimethylbenzene
Chemosphere,40:893-896 2000(Apr.)
Author:Yuji Tsujimoto, Tsutomu Noda, Mitsuru Shimizu, Hiroshi Moriwaki, Masanobu Tanaka
Abstract:The trimethylbenzenes that are used extensively as organic solvents are known as indoor pollutant, therefore it is important to reveal their influences on human bodies. This article describes the dimethylbenzyl mercapturic acid, as a urinary metabolite of 1,2,4-trimethylbenzenes, was detected from the urine of rats administered with 1,2,4-trimethylbenzenes.
Keywords:メルカプツール酸・尿中代謝物


Determination of 8-Hydroxy-2’-deoxyguanosine in urine by liquid chromatography-electrospray ionization- mass spectrometry
Analytical Sciences,16:105-106 2000(Feb.)
Author:Hiroshi Moriwaki
Abstract:When DNA is attacked by hydroxy radical, especially C8 of guanine base is hydroxylated. This hydroxylated guanine base is cut and emitted into the bloodstream as 8-OHdG. This is to say the amount of 8-OHdG within urine is a good barometer to see the stress level caused by hydroxy radical in a living organism. Within this article, easy method to measure 8-OHdG in urine by LC/MS has been developed.
Keywords:尿中代謝物・LC/MS

Conformational specificity in photoinduced intramolecular 1,7-hydrogen abstraction of homonaphthoquinones with a spiro-linked dibenzocycloheptene ring
Organic Lett,2:559-562 2000(Feb.)
Author:Ken Kokubo, Kazuhito Kawahara, Toshihiko Takatani, Hiroshi Moriwaki, Tatsuya Kawamoto, Takumi Oshima
Abstract:An observation was made on the photoreaction of homonaphthoquinone with spiro-linked diphenylcyclopropane rings. The carbonyl group of naphthoquinone was photoexcited and pulled hydrogen from ethano spiro-linked portion. Furthermore, it was disclosed,that carbon from ethano portion and carbonyl carbon bind themselves within intramolecular reaction. Also, it has been revealed that this reaction differs owed to the configuration of ethano spiro-linked portion.
Keywords:光反応・シクロプロパン


Electrospray mass spectrometric determination of 1-nitropyrene and non-substituted polycyclic aromatic hydrocarbons using tropylium cation as a post-column HPLC reagent
Analyst,125:417-420 2000(Feb.)
Author:Hiroshi Moriwaki
Abstract:The polycyclic aromatic hydrocarbons reveal carcinogens and exist widely in the environment as pollutant. Conventionally, it had been difficult to detect polycyclic aromatic hydrocarbons by using LC/MS. There were few examples of detection of PAHs by ESI method, because the polarity of PAHs were low. This article describes the trace analysis of PAHs with LC/MS, by having tropylium cation as a post-column HPLC reagent, since it interacts with PAHs.
Keywords:LC/MS

Identification of the dimethylbenzyl mercapturic acid in urine of rats treated with 1,2,3-trimethylbenzene
Chemosphere,39:725-730 1999(Aug.)
Author:Yuji Tsujimoto, Tsutomu Noda, Mitsuru Shimizu, Hiroshi Moriwaki, Masanobu Tanaka
Abstract:The trimethylbenzenes that are used extensively as organic solvents are known as indoor pollutant, therefore it is important to reveal their influences on human bodies. This article describes that dimethylbenzyl mercapturic acid as a urinary metabolite of 1,2,3-trimethylbenzenes. It was detected from the urine of rats administered with 1,2,3-trimethylbenzenes.
Keywords:メルカプツール酸・尿中代謝物


Cation complexation of quinocrown ethers in electrospray ionization mass spectrometry. A comparison with benzocrown ethers
J. Chem. Soc., Perkin Trans. 2,:1235-1240 1999(Jun.)
Author:Akihiko Tsuda, Hiroshi Moriwaki, Takumi Oshima
Abstract:By using ESI (Electrospray Ionization) method, a comparing investigation was made between cation complexation of quinocrown and cation complexation of benzocrown ethers. Quinocrown ethers are constructed by adopting crown ethers onto quinone skeleton, which possesses electron-withdrawing capability. When quinocrown ethers are ring-fused with quinone, it forms distortion, revealing how quinocrown ethers have less metallic affinity than benzocrown ethers. The new knowledge was acquired regarding the influence on cation complexation due to the structures of crown ethers.
Keywords:超分子・LC/MS

Synthesis of regioisomeric dimethylbenzyl mercapturic acids anticipated from the metabolism of 1,2,3-trimethylbenzene
Chemosphere,38:2065-2070 1999(Apr.)
Author:Yuji Tsujimoto, Tsutomu Noda, Mitsuru Shimizu, Hiroshi Moriwaki, Masanobu Tanaka
Abstract:The trimethylbenzenes that are used extensively as organic solvents, are known as indoor pollutant, therefore it is important to reveal their influences on human bodies. This article describes the synthetic method of dimethylbenzyl mercapturic acid, which is expected to be urinary metabolite of 1,2,3-trimethylbenzenes. By this method, the effective synthesis of these estimated metabolites has become possible.
Keywords:メルカプツール酸・尿中代謝物


Electrospray mass spectrometric determination of polycyclic aromatic hydrocarbons by detecting the p-p complexes with tropylium cation
Analytical Communication,36:53-56 1999(Feb.)
Author:Hiroshi Moriwaki, Akira Imaeda, Ryuichi Arakawa
Abstract:It is difficult to detect polycyclic aromatic hydrocarbons directly byESI/MS. By using how polycyclic aromatic hydrocarbons possess electrons, tropylium cation with π-π interaction was added to them, so that tropylium cation can form π-π complexes with PAHs. The method to detect such complexes by ESI/MS has been developed. By adding tropylium cation after a column separation, an analysis of PAHs by LC/MS has become possible.
Keywords:LC/MS

High performance liquid chromatographic determination of mercapturic acids in urine of rats administered with m- or p-xylene
Chemosphere,36:2491-2495 1998(May)
Author:Yuji Tsujimoto, TsutomuNoda, Hiroshi Moriwaki, Masanobu Tanaka
Abstract:Xylene is a volatile organic compound which has high concentration of pollutant in indoors. The methylbenzyl mercapturic acid is an urine metabolite of xylene, and the method to measure it in urine has been developed. HPLC has been used for the analysis. Xylene was administered to a rat and its collected urine was put through solid-phase extraction, before the analysis by HPLC was carried out. It was made clear that the discharge rate of these substances is actually slower by far than expected.
Keywords:メルカプツール酸・尿中代謝物


Synthesis and characterization of m-　and　p-　methylbenzyl mercapturic acids derived from m-and p-xylenes
Chemosphere,34:2519-2523 1997(Jun.)
Author:Masanobu Tanaka, Tsutomu Noda, Hiroshi Moriwaki, Yuji Tsujimoto
Abstract:Xylene is known as a major indoor pollutant, therefore it is important to understand its influence on human bodies. Within this article, the synthetic method of a glutathione conjugate of m-, p-xylenes, the m-, p-methylbenzyl mercapturic acids has been indicated. The m-, p-methylbenzyl mercapturic acid is expected to be an urine metabolite of m-, or p-xylenes. By this method, the effective synthesis of these expected metabolites has become possible.
Keywords:メルカプツール酸・尿中代謝物


Half-chair conformation of trans-1,4-dimethyl-7,7-diphenylbicyclo[4.1.0]hepta-2,5-dione
Acta Cryst.,C52:2269-2271 1996(Nov.)
Author:Hiroshi Moriwaki, Tatsuya Kawamoto,Takumi Oshima
Abstract:The conformation of trans-1,4-dimethyl-7,7-diphenylbicyclo[4.1.0]hepta-2,5-dione, which generates from a reaction between homobenzoquinone and triethylamine has been determined. It has been discovered, that this substance takes a "half-chair conformation."
Keywords:光誘起電子移動反応・シクロプロパン


Photoreaction of homobenzoquinones with amine donors
J. Chem. Soc. Perkin Trans. 1,:1461-1466 1996(Jun.)
Author:Hiroshi Moriwaki, Takashi Matsumoto, Toshikazu Nagai, Takumi Oshima
Abstract:The photoinduced electron transfer reaction has occured between amine donors and homobenzoquinone, which has methyl group displaced benzoquinone with ring-fused cyclopropane. From amine doners, an addition reaction of hydrogens occurred to the double bond regions of homobenzoquinone. A ring-opening reaction of cyclopropane such as homonaphthoquinone did not occur. By examining the mechanism of this reaction through MOPAC, the difference in reaction compared to homonaphthoquinone has been described.
Keywords:光誘起電子移動反応・シクロプロパン


Conversion of donor-substituted homonaphthoquinones to indenonaphthoquinones via intramolecular photoinduced electron-transfer
J. Chem. Soc., Chem. Commun.,:495-496 1996(Feb.)
Author:Hiroshi Moriwaki, Takumi Oshima, Toshikazu Nagai
Abstract:A new example regarding the intramolecular photoinduced electron transfer has been presented. Irradiation of anisyl homonaphthoquinone in the presence of magnesium ion resulted in the quantitative formation of indenonaphthoquinone. When there was no magnesium, this reaction did not proceed at all. It is considered, that after the intramolecular electron transfer occured from anisyl group to naphthoquinone skelton, the charge separation was stabilized by magnesium, triggering cleavage reaction of cyclopropane and resulting in the reaction.
Keywords:光誘起電子移動反応・シクロプロパン


Photoinduced electron-transfer reactions of homonaphthoquinones with amine and arene donors
J. Chem. Soc. Perkin Trans. 1,:2517-2523 1995(Oct.)
Author:Hiroshi Moriwaki, Takumi Oshima, Toshikazu Nagai
Abstract:An unique reaction would be expected from cyclopropane, due to its large steric distortion. In this article, photoinduced electron-transfer was carried out between various kinds of donors and homonaphthoquinones, which are constructed from naphthoquinone skeleton with electron-withdrawing capability and ring-fused cyclopropane. An observation was made from the perspective of reaction mechanism, concerning what causes different donors to produce largely different products. Furthermore, it was made clear, that different reactions have occured, when the substituent of cyclopropane are changed.
Keywords:光誘起電子移動反応・シクロプロパン


Photoreduction of carbon dioxide using chalcogenide semiconductor microcrystals
J. Photochem. Photobiol. A,86:191-196 1995(Feb.)
Author:Hiroshi Inoue, Hiroshi Moriwaki, Kotaro Maeda, Hiroshi Yoneyama
Abstract:The amount of carbon dioxide emission has been increasing and its influence on the environment is of great concern. It is desirable to exchange carbon dioxide into useful substances and use them. In this article, by using semiconductor ultrafine particles of ZnS or CdS, metals from zinc or cadmium are deposited on the surface of ultrafine particles. Then, composites of metals and semiconductor ultrafine particles are adjusted in order to use them for the photoreduction of carbon dioxide as photocatalyst.
Keywords:光誘起電子移動反応・シクロプロパン


Photoisomerization of bromonaphthoquinone-fused diphenylcyclopropane into xanthylium salt in the presence of arene donors
J. Chem. Soc., Chem. Commun.,:1681-1682 1994(Jul.)
Author:Hiroshi Moriwaki, Takumi Oshima, Toshikazu Nagai
Abstract:The cleavage reaction of carbon-carbon bond, which possesses large steric distortion is expected for many applications in various reactions. In this article, photoinduced electron-transfer was carried out between arene donors and homonaphthoquinones, which were constructed from naphthoquinone skeleton with electron-withdrawing capability and ring-fused cyclopropane. Under the coexistence with naphthalene, when irradiated with light, homonaphthoquinone produced xanthylium. This was considered to have happened, due to the mechanism which was produced by aryl cation after the ring-opening reaction.
Keywords:光誘起電子移動反応・シクロプロパン


Photoinduced reductive cleavage of diarylcyclopropanes fused with bromonaphthoquinone in the presence of amines
J. Chem. Soc., Chem. Commun.,:255-256 1994(Feb.)
Author:Hiroshi Moriwaki, Takumi Oshima, Toshikazu Nagai
Abstract:An unique reaction is expected from cyclopropane due to its large steric distortion. In this article, photoinduced electron-transfer was carried out between amines and homonaphthoquinones, which were constructed from naphthoquinone skeleton with electron-withdrawing capability and ring-fused cyclopropane. Under the coexistence with triethylamine, when irradiated with light, homonaphthoquinone produced dimer. This was considered to have happened, due to the mechanism which was produced by aryl radical after the ring-opening reaction.
Keywords:光誘起電子移動反応・シクロプロパン


Presentations
Removal of PCB and POPs from soil using the composite material containing iron and activated carbon powder in the freezing-dried calcium alginate matrix
The 9th International PCB Workshop 2016
Presenter:Hiroshi Moriwaki, Mako Funada, Takeshi Nakano

Electrospray mass spectrometric observation of the interaction between environmental pollutants and biologic compounds
19th International Mass Spectrometry Conference 2012
Presenter:Hiroshi Moriwaki, Takahiro Nakagawa

Long-range transport of atmospheric pollutants to Japan and remote islands in Japan
Techno-Ocean 2010 2010
Presenter:H. Moriwaki, K. Haraguchi, K. Katahira, H. Yamazaki, S. Yoshikawa

Themes for Joint Research: Environmental monitoring
Instrumental Analysis
Geology
MALDI/TOF/MS
LC/MS

Research Grants: Grants‐in‐aid for Scientific Research(Research Representative)
2017 - 2019 , 枯草菌遺伝子操作株を滅菌・凍結乾燥させた粉体によるレアメタルの抽出法の開発

Grants‐in‐aid for Scientific Research(Researcher)
2007 - 2008 , 閉鎖水域堆積物の微小球状粒子を用いた東アジア越境大気汚染の長期時空間変動解析
2004 - 2004 , 長期歴史トレンド解析による大陸起源大気汚染物質長距離輸送の環境影響評価
2002 - 2004 , 不法投棄廃棄物などに含まれる科学物質の包括的計測手法の開発に関する研究

Other Research Grants
2006 - 2007 , 都市の地盤環境に関わる基盤的研究－大阪平野表層部の地層形成過程と地盤環境特性の解明－

Other
2008 - 2008 , 環境汚染による複合的な生体影響の評価－バイオテクノロジーで複合汚染を見る－

Education

Lectures: 2020 , Analytical chemistry
2020 , Lecture for environmental chemistry
2020 , Environmental chemistry

Shinshu University Researcher Directory

Moriwaki Hiroshi

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Moriwaki　Hiroshi