Doctor of Agriculture, Kyoto University, 1991

Reproductive biology

Cryobiology

Developmental engineering

Euglycemic control (Diabetes)

1984 - 1986, Graduate School, Okayama University, Division of Agriculture, Course of Animal Science

1980 - 1984, Okayama University, Faculty of Agriculture, Department of Animal Science

2019 - 2022
Governor, Society for Reproduction and Development (SRD)

2009 - 2018
Board, Society for Reproduction and Development (SRD)

2008 - 2025
Editorial Board, Theriogenology (Elsevier Inc.)

2008 - 2023
Editorial Board, Journal of Reproduction and Development (IPEC Inc.)

2007 - 2018
Board, Japanese Society for Ova Research (JSOR)

2006
Japanese Association for Experimental Animal Technologists: 25th Award for Research Encouragement

2002
Japanese Society for Animal Reproduction: Award for Outstanding Research

Detection of sow pregnancy in day-20 urine samples using monoclonal antibody against synthesized porcine early pregnancy factor: Preliminary results
S. Park, E-S. Cho, C-H. Kim, S. Lee, Y-D. Jeong, M. Park, D. Kim, D. Seo, Y-H. Kim, S. Hochi, I. Choi, H-J. Chung
Theriogenology, 229, 23-29, Nov. 2024, Refereed

Lower developmental potential of rat zygotes produced by ooplasmic injection of testicular spermatozoa versus cauda epididymal spermatozoa
M. Ide, I. Saito, M. Sanbo, M. Kanatsu-Shinohara, T. Shinohara, M. Hirabayashi, S. Hochi
J. Reprod. Dev., 70(4), 254-258, Aug. 2024, Refereed
Last, Corresponding

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos
W-J. Jiang, S-H. Lee, G. Heo, H-J. Chung, E-S. Cho, S-J. Sa, S. Hochi, X-S. Cui
Front. Cell Dev. Biol., 11, 1238546, Oct. 2023, Refereed

Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
K. Iwatsuki, M. Oikawa, H. Kobayashi, C.A. Penfold, M. Sanbo, T. Yamamoto, S. Hochi, K. Kurimoto, M. Hirabayashi, T. Kobayashi
Cell Rep. Methods, 3(8), 100542, Aug. 2023, Refereed

Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10
S-W. Park, Y-M. Kim, M-J. Lee, D-Y. Jeon, S-J. Sa, Y-D. Jeong, H-S. Seong, S. Hochi, E-S. Cho, H-J. Chung
J. Anim. Sci. Technol., 65(2), 401-411, Mar. 2023, Refereed

Characterization of homozygous Foxn1 mutations induced in rat embryos by different delivery forms of Cas9 nuclease
T. Goto, K. Yogo, S. Hochi, M. Hirabayashi
Mol. Biol. Rep., 50(2), 1231-1239, Feb. 2023, Refereed

High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection
S. Hochi, M. Ide, S. Ueno, M. Hirabayashi
J. Reprod. Dev., 68(5), 335-339, Aug. 2022, Refereed
Lead, Corresponding

Fabrication of functional rat pseudo-islets after cryopreservation of pancreatic islets or dispersed islet cells
K. Nakayama-Iwatsuki, M. Hirabayashi, S. Hochi
J. Tissue Eng. Regen. Med., 15(7), 686-696, May 2021, Refereed
Last, Corresponding

All-in-One Silk Fibroin Sponge as the Vitrification Cryodevice of Rat Pancreatic Islets and the VEGF-Embedded Scaffold for Subrenal Transplantation
T. Yamanaka, K. Nakayama-Iwatsuki, S. Fujimoto, N. Hirono, J. Negishi, Y. Tamada, M. Hirabayashi, S. Hochi
Transplant. Proc., 53(5), 1744-1750, Apr. 2021, Refereed
Last, Corresponding

Acellular matrix derived from rat liver improves the functionality of rat pancreatic islets before and after vitrification
K. Nakayama-Iwatsuki, K. Yanagisawa, D. Tanaka, M. Hirabayashi, J. Negishi, S. Hochi
Cryobiology, 100, 90-95, Mar. 2021, Refereed
Last, Corresponding

Altrenogest affects expression of galectin-3 and fibroblast growth factor 9 in the reproductive tract of sows
S-Y. Baek, S-J. Sa, Y-D. Jeong, E-S. Cho, J-G. Hong, Y-S. Kim, K-H. Cho, S-H. Park, K-W. Kim, H-C. Lee, S. Hochi, S-H. Lee, I-C. Choi, H-J. Chung
Anim. Biotechnol., 32(5), 537-543, Feb. 2021, Refereed

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
T. Kobayashi, T. Goto, M. Oikawa, M. Sanbo, F. Yoshida, R. Terada, N. Niizeki, N. Kajitani, K. Kazuki, Y. Kazuki, S. Hochi, H. Nakauchi, M.A. Surani, M. Hirabayashi
Nat. Commun., 12(1), 1328, Feb. 2021, Refereed

Rescue of vitrified-warmed bovine mature oocytes by short-term recovery culture with resveratrol
S. Chinen, T. Yamanaka, M. Hirabayashi, S. Hochi
Cryobiology, 97, 185-190, Dec. 2020, Refereed
Last, Corresponding

Transplantation of rat pancreatic islets vitrified-warmed on the nylon mesh device and the silk fibroin sponge disc
K. Nakayama-Iwatsuki, T. Yamanaka, J. Negishi, J. Teshima, Y. Tamada, M. Hirabayashi, S. Hochi
Islets, 12(6), 145-155, Dec. 2020, Refereed
Last, Corresponding

Silk fibroin sheet multilayer suitable for vitrification of in vitro-matured bovine oocytes
K. Nakayama, S. Chinen, J. Teshima, Y. Tamada, M. Hirabayashi, S. Hochi
Theriogenology, 145, 109-114, Mar. 2020, Refereed
Last, Corresponding

【論119】 Nylon mesh cryodevice for bovine mature oocytes, easily removable excess vitrification solution
S. Chinen, T. Yamanaka, K. Nakayama, H. Watanabe, Y. Akiyama, M. Hirabayashi, S. Hochi
Cryobiology, 90, 96-99, Oct. 2019, Refereed
Last, Corresponding

【論118】 Production of human tissue-type plasminogen activator (htPA) using in vitro cultured transgenic pig mammary gland cells
H-J. Chung, H-J. Park, S-Y. Baek, J-K. Park, W-Y. Lee, K-W. Kim, Y-M. Jo, S. Hochi, Y-M. Kim, T-J. Choi, E-S. Cho, K-H. Cho
Anim. Biotechnol., 30(4), 317-322, Aug. 2019, Refereed

【論117】 Supplementary cryoprotective effect of carboxylated ε-poly-L-lysine during vitrification of rat pancreatic islets
K. Nakayama, T. Yamanaka, Y. Tamada, M. Hirabayashi, S. Hochi
Cryobiology, 88, 70-74, Jun. 2019, Refereed
Last, Corresponding

【論116】 Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats
T. Goto, H. Hara, M. Sanbo, H. Masaki, H. Sato, T. Yamaguchi, S. Hochi, T. Kobayashi, H. Nakauchi, M. Hirabayashi
Nat. Commun., 10(1), 451, Feb. 2019, Refereed

【論115】 FGF2 has distinct molecular functions from GDNF in the mouse germline niche
K. Masaki, M. Sakai, S. Kuroki, J. Jo, K. Hoshina, Y. Fujimori, K. Oka, T. Amano, T. Yamanaka, M. Tachibana, Y. Tabata, T. Shinozawa, O. Ishizuka, S. Hochi, S. Takashima
Stem Cell Rep., 10(6), 1782-1792, Jun. 2018, Refereed

【論114】 Haploid embryonic stem cell lines derived from andorogenetic and parthenogenetic rat blastocysts
M. Hirabayashi, H. Hara, T. Goto, A. Takizawa, M.R. Dwinell, T. Yamanaka, S. Hochi, H. Nakauchi
J. Reprod. Dev., 63(6), 611-616, Dec. 2017, Refereed

【論113】 Nylon mesh device for vitrification of large quantities of rat pancreatic islets
T. Yamanaka, T. Goto, M. Hirabayashi, S. Hochi
Biopreserv. Biobank., 15(5), 457-462, Oct. 2017, Refereed
Last, Corresponding

【論112】 Downsizing cumulus cell layers to improve cryotolerance of germinal vesicle-stage bovine oocytes
K. Tashima, Y. Kubo, M. Hirabayashi, S. Hochi
Theriogenology, 95, 1-7, Jun. 2017, Refereed
Last, Corresponding

【論111】 Direct comparison of Cryotop® vitrification and Bicell® freezing on recovery of functional rat pancreatic islets
T. Yamanaka, K. Tashima, R. Takahashi, S. Takashima, T. Goto, M. Hirabayashi, S. Hochi
Cryobiology, 73(3), 376-382, Dec. 2016, Refereed
Last, Corresponding

【論110】 Hypomorphic phenotype of Foxn1 gene-modified rats by CRISPR/Cas9 system
T. Goto, H. Hara, H. Nakauchi, S. Hochi, M. Hirabayashi
Transgenic Res., 25(4), 533-544, Aug. 2016, Refereed

【論109】 Rat blastocysts from nuclear injection and time-lagged enucleation and their commitment to embryonic stem cells
H. Hara, T. Goto, A. Takizawa, H.J. Jacob, M. Sanbo, T. Kobayashi, H. Nakauchi, S. Hochi, M. Hirabayashi
Cell. Reprogram., 18(2), 108-115, Apr. 2016, Refereed

【論108】 Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries
H. Hara, M. Tagiri, M. Hirabayashi, S. Hochi
Zygote, 24(1), 115-120, Feb. 2016, Refereed

【論107】 High incidence of polyspermic fertilization in bovine oocytes matured in vitro after Cryotop vitrification
I-S. Hwang, D-J. Kwon, G-S. Im, K. Tashima, S. Hochi, S. Hwang
Cryo Letters, 37(1), 27-33, Jan. 2016, Refereed

【論106】 Knock-in of a histone H2B-tdTomato reporter into the Rosa26 locus allows visualization of cell nuclei in rats
T. Goto, T. Kobayashi, H. Hara, M. Sanbo, S. Hochi, H. Nakauchi, M. Hirabayashi
Mol. Reprod. Dev., 82(12), 916-917, Dec. 2015, Refereed

【論105】 High revivability of vitrified-warmed bovine oocytes during recovery culture with α-tocopherol
I. Yashiro, M. Tagiri, H. Ogawa, K. Tashima, S. Takashima, H. Hara, M. Hirabayashi, S. Hochi
Reproduction, 149(4), 347-355, Apr. 2015, Refereed

【論104】 Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization
H. Hara, I. Yamane, I. Noto, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi
Zygote, 22(4), 476-482, Nov. 2014, Refereed

【論103】 Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa
H. Hara, M. Tagiri, I-S. Hwang, M. Takahashi, M. Hirabayashi, S. Hochi
Cryobiology, 68(3), 354-360, Jun. 2014, Refereed

【論102】 Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines
M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, S. Hochi
J. Reprod. Dev., 60(1), 78-82, Mar. 2014, Refereed

【論101】 Derivation of embryonic stem cell lines from parthenogenetically developing rat blastocysts
M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, M. Kato-Itoh, H. Sato, T. Kobayashi, H. Nakauchi, S. Hochi
Stem Cells Dev., 23(2), 107-114, Jan. 2014, Refereed

【論100】 Rescue of vitrified-warmed bovine oocytes with Rho-associated coiled-coil kinase inhibitor
I-S. Hwang, H. Hara, H-J. Chung, M. Hirabayashi, S. Hochi
Biol. Reprod., 89(2), 26, Aug. 2013, Refereed

【論99】 A retrospective analysis of germline competence in rat embryonic stem cell lines
M. Hirabayashi, C. Tamura, M. Sanbo, M. Kato-Itoh, T. Kobayashi, H. Nakauchi, S. Hochi
Transgenic Res., 22(2), 411-416, Apr. 2013, Refereed

【論98】 Proteins associated with reproductive disorders in testes of human erythropoietin gene-harboring transgenic boars
M-S. Choi, M-R. Shim, M-Y. Oh, K-W. Kim, H-C. Lee, B-C. Yang, H-K. Chung, J-H. Kim, H-T. Lee, I-S. Hwang, S. Hochi, Y-T. Heo, N-H. Kim, S-J. Uhm, J-K. Park, W-K.Chang, H-J. Chung
Theriogenology, 78(5), 1020-1029, Sep. 2012, Refereed

【論97】 Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells
M. Hirabayashi, C. Tamura, M. Sanbo, T. Goto, M. Kato, T. Kobayashi, H. Nakauchi, S. Hochi
Mol. Reprod. Dev., 79(6), 402-412, Jun. 2012, Refereed

【論96】 High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization
H. Hara, I-S. Hwang, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi
Theriogenology, 77(5), 908-915, Mar. 2012, Refereed

【論95】 Birefringent parameter available for quantitative analysis of human zona hardness
H. Iwayama, S. Hochi, M. Yamashita
Zygote, 19(4), 323-329, Nov. 2011, Refereed

【論94】 Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center
H. Hara, H. Abdalla, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi
J. Reprod. Dev., 57(3), 428-432, Jun. 2011, Refereed

【論93】 In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification
H. Iwayama, S. Hochi, M. Yamashita
J. Assist. Reprod. Genet., 28(4), 355-361, Apr. 2011, Refereed

【論92】 Low stretching ability of human ooplasma during piezo-ICSI as a risk factor on post-injection survival and implantation
H. Iwayama, S. Hochi, M. Yamashita
J. Mammal. Ova Res., 27(3), 150-156, Oct. 2010, Refereed

【論91】 Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
H. Abdalla, M. Shimoda, H. Hara, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi
Theriogenology, 74(6), 1028-1035, Oct. 2010, Refereed

【論90】 Rat transgenesis via embryonic stem cells electroporated with Kusabira-orange gene
M. Hirabayashi, M. Kato, M. Sanbo, T. Kobayashi,S. Hochi, H. Nakauchi
Mol. Reprod. Dev., 77(6), 474, Jun. 2010, Refereed

【論89】 Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
S. Hochi, H. Abdalla, M. Shimoda, H. Hara, H. Morita, M, Kuwayama, M. Hirabayashi
Theriogenology, 73(8), 1139-1145, May 2010, Refereed

【論88】 Establishment of rat embryonic stem cell lines that can participate into germline chimerae at high efficiency
M. Hirabayashi, M. Kato, T. Kobayashi, M. Sanbo, T. Yagi, S. Hochi, H. Nakauchi
Mol. Reprod. Dev., 77(2), 94, Feb. 2010, Refereed

【論87】 Impaired active demethylation of paternal genome in pronuclear-stage rat zygotes produced by in vitro ferilization or intracytoplasmic sperm injection
Y. Yoshizawa, M. Kato, M. Hirabayashi, S. Hochi
Mol. Reprod. Dev., 77(1), 69-75, Jan. 2010, Refereed

【論86】 A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
H. Abdalla, M. Shimoda, M. Hirabayashi, S. Hochi
Theriogenology, 72(4), 453-460, Sep. 2009, Refereed

【論85】 Demethylation dynamics of paternal genome in pronuclear-stage bovine zygotes produced by in vitro fertilization and ooplasmic injection of freeze-thawed or freeze-dried spermatozoa
H. Abdalla, M. Hirabayashi, S. Hochi
J. Reprod. Dev., 55(4), 433-439, Aug. 2009, Refereed

【論84】 Activation regimens for full-term development of rabbit oocytes injected with round spermatids
M. Hirabayashi, M. Kato, K. Kitada, N. Ohnami, M. Hirao, S. Hochi
Mol. Reprod. Dev., 76(6), 573-579, Jun. 2009, Refereed

【論83】 Availability of subfertile transgenic rats expressing c-myc gene as recipients for spermatogonial transplantation
M. Hirabayashi, Y. Yoshizawa, M. Kato, T. Tsuchiya, S. Nagao, S. Hochi
Transgenic Res., 18(1), 135-141, Feb. 2009, Refereed

【論82】 The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis
H. Abdalla, M. Hirabayashi, S. Hochi
Theriogenology, 71(3), 543-552, Feb. 2009, Refereed

【論81】 Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids
M. Hirabayashi, M. Kato, S. Hochi
Exp. Anim., 57(4), 401-405, Jul. 2008, Refereed

【論80】 Effect of post-IVF developmental kinetics on in-vitro survival of vitrified-warmed domestic cat blastocysts
T.Tsujioka, C. Otzdorff, J. Braun, S. Hochi
Reprod. Domest. Anim., 43(3), 323-327, Jun. 2008, Refereed

【論79】 Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
S. Hochi, K. Watanabe, M. Kato, M. Hirabayashi
Mol. Reprod. Dev., 75(5), 890-894, May 2008, Refereed

【論78】 Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats
M. Hirabayashi, M. Kato, K. Amemiya, S. Hochi
Exp. Anim., 57(2), 145-148, Apr. 2008, Refereed

【論77】 Follicle size-dependent changes of follicular fluid components and oocyte diameter in Antarctic minke whales (Balaenoptera bonaerensis)
H. Nagai, T. Mogoe, H. Ishikawa, S. Hochi, S. Ohsumi, Y. Fukui
J. Reprod. Dev., 53(6), 1265-1272, Dec. 2007, Refereed

【論76】 Attempt at intracytoplasmic sperm injection to in vitro matured oocytes in common minke whales (Balaenoptera acutorostrata) captured at Kushiro coast survey
Y. Fukui, H. Iwayama, T. Matsuoka, H. Nagai, N. Koma, T. Mogoe, H. Ishikawa, Y.Fujise, M. Hirabayashi, S. Hochi, H. Kato, S. Ohsumi
J. Reprod. Dev., 53(4), 945-952, Aug. 2007, Refereed

【論75】 Effect of enucleation on inactivation of cytostatic factor activity in matured rat oocytes
J. Ito, M. Kato, S. Hochi, M. Hirabayashi
Cloning Stem Cells, 9(2), 257-266, Jul. 2007, Refereed

【論74】 The ability of whale haploid spermatogenic cells for inducing calcium oscillations and its relevance to oocyte activation
K. Amemiya, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi
Zygote, 15(2), 103-108, May 2007, Refereed

【論73】 Conventional freezing of in vitro produced and biopsied bovine blastocysts in the presence of low concentration glycerol with sucrose
K. Tominaga, F. Iwaki, S. Hochi
J. Reprod. Dev., 53(2), 443-447, Apr. 2007, Refereed

【論72】 Quality analysis of buffalo blastocysts derived from oocytes vitrified before and after enucleation and reconstructed with somatic cell nuclei
S. Muenthaisong, C. Laowtammathron, M. Katudat-Cairns, R. Parnpai, S. Hochi
Theriogenology, 67(4), 893-900, Mar. 2007, Refereed

【論71】 Involvement of Ca(2+)-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of rat matured oocytes
J. Ito, M. Shimada, S. Hochi, M. Hirabayashi
Theriogenology, 67(3), 475-485, Feb. 2007, Refereed

【論70】 Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection
T. Fujihira, M. Kobayashi, S. Hochi, M. Hirabayashi, H. Ishikawa, S. Ohsumi, Y. Fukui
Zygote, 14(2), 89-95, May 2006, Refereed

【論69】 No effect of recombinase-mediated DNA transfer on production efficiency of transgenic rats
M. Hirabayashi, M. Kato, R. Kaneko, T. Hirabayashi, M. Morira, S. Hochi
Exp. Anim., 55(2), 131-135, Apr. 2006, Refereed

【論68】 Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)
T. Kobayashi, K. Amemiya, K. Takeuchi, T. Tsujioka, K. Tominaga, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi
Zygote, 14(1), 45-51, Feb. 2006, Refereed

【論67】 Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR
Y. Iwanami, T. Kobayashi, M. Kato, M. Hirabayashi, S. Hochi
Theriogenology, 65(2), 288-298, Jan. 2006, Refereed

【論66】 Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer
K. Tominaga, Y. Hamada, S. Hochi
J. Mammal. Ova Res., 22(3), 178-184, Oct. 2005, Refereed

【論65】 Factors affecting cryosurvival of nuclear-transferred bovine and swamp buffalo blastocysts: effects of hatching stage, linoleic acid-albumin in IVC medium and Ficoll supplementation to vitrification solution
C. Laowtammathron, C. Lorthongpanich, M. Ketudat-Cairns, S. Hochi, R. Parnpai
Theriogenology, 64(5), 1185-1196, Sep. 2005, Refereed

【論64】 Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA
M. Hirabayashi, M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi
Mol. Reprod. Dev., 70(4), 422-428, Apr. 2005, Refereed

【論62】 Contribution of high p34(cdc2) kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes
J. Ito, M. Hirabayashi, M. Kato, A. Takeuchi, M. Ito, M. Shimada, S. Hochi
Reproduction, 129(2), 171-180, Feb. 2005, Refereed

【論63】 Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
M. Hirabayashi, M. Kato, J. Ito, S. Hochi
Zygote, 13(1), 79-85, Feb. 2005, Refereed

【論61】 Effects of cryodevice type and donors' sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicle-stage
H. Iwayama, S. Hochi, M. Kato, M. Hirabayashi, M. Kuwayama, H. Ishikawa, S. Ohsumi, Y. Fukui
Zygote, 12(4), 333-338, Nov. 2004, Refereed

【論59】 Production of transgenic rats by ooplasmic injection of spermatogenic cells exposed to exogenous DNA: A preliminary study
M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi, M. Hirabayashi
Mol. Reprod. Dev., 69(2), 153-158, Oct. 2004, Refereed

【論60】 Acquirement of oocyte activating factor in Antarctic minke whale (Balaenopterabonaerensis) spermatogenic cells, assessed by meiosis resumption of microinseminated mouse oocytes
K. Amemiya, Y. Iwanami, T. Kobayashi, T. Terao, Y. Fukui, H. Ishikawa, S. Ohsumi, M. Hirabayashi, S. Hochi
J. Mammal. Ova Res., 21(3), 149-156, Oct. 2004, Refereed

【論58】 Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts
M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi
J. Reprod. Dev., 50(2), 191-195, Apr. 2004, Refereed

【論57】 Effect of activation regimens for rat oocytes on full-term development after round spermatid injection
M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi
Contem. Top. Lab. Anim. Sci., 43(2), 13-15, Mar. 2004, Refereed

【論56】 Successful vitrification of pronuclear-stage rabbit zygotes by minimum volume cooling procedure
S. Hochi, T. Terao, M. Kamei, M. Kato, M. Hirabayashi, M. Hirao
Theriogenology, 61(2-3), 267-275, Jan. 2004, Refereed

【論55】 Factors affecting premature chromosome condensation of cumulus cell nuclei injected into rat oocytes
M. Hirabayashi, M. Kato, A. Takeuchi, A. Ishikawa, S. Hochi
J. Reprod. Dev., 49(2), 121-126, Apr. 2003, Refereed

【論54】 Factors influencing chromosome condensation and development of cloned rat embryos
M. Hirabayashi, M. Kato, A. Ishikawa, S. Hochi
Cloning Stem Cells, 5(1), 35-42, 2003, Refereed

【論53】 Rescue of infertile transgenic rat lines by intracytoplasmic injection of cryopreserved round spermatids
M. Hirabayashi, M. Kato, T. Aoto, M. Ueda, S. Hochi
Mol. Reprod. Dev., 62(3), 295-299, Jul. 2002, Refereed

【論52】 Effect of Ca(2+)/Mg(2+)-free culture condition on spontaneous first cleavage of rat oocytes
A. Takeuchi, M. Kato, K. Ito, K. Kimura, A. Hanada, M. Hirabayashi, S. Hochi
J. Reprod. Dev., 48(3), 243-248, Jun. 2002, Refereed

【論51】 Offspring derived from intracytoplasmic injection of transgenicrat sperm
M. Hirabayashi, M. Kato, T. Aoto, A. Sekimoto, M. Ueda, I. Miyoshi, N. Kasai, S. Hochi
Transgenic Res., 11(2), 221-228, Apr. 2002, Refereed

【論50】 Strontium-induced activation regimen for rat oocytes in somatic cell nuclear transplantation
M. Kato, M. Hirabayashi, T. Aoto, K. Ito, M. Ueda, S. Hochi
J. Reprod. Dev., 47(6), 407-413, Dec. 2001, Refereed

【論48】 Effects of cryopreservation of pronuclear-stage rabbit zygotes on the morphological survival, blastocyst formation, and full-term development after DNA microinjection
S. Hochi, M. Hirabayashi, M. Hirao, M. Kato, T. Kobayashi, K. Kimura, K. Hirasawa, S.P. Leibo, M. Ueda
Mol. Reprod. Dev., 60(2), 227-232, Oct. 2001, Refereed

【論49】 Production of transgenic rats using young Sprague-Dawley females treated with PMSG and hCG
M. Hirabayashi, K. Ito, A. Sekimoto, S. Hochi, M. Ueda
Exp. Anim., 50(5), 365-369, Oct. 2001, Refereed

【論47】 A comparative study on the integration of exogenous DNA into mouse, rat, rabbit, and pig genomes
M. Hirabayashi, R. Takahashi, K. Ito, N. Kashiwazaki, M. Hirao, K. Mirasawa, S. Hochi, M. Ueda
Exp. Anim., 50(2), 125-131, Apr. 2001, Refereed

【論46】 Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes
S. Hochi, M. Akiyama, G. Minagawa, K. Kimura, A. Hanada
Cryobiology, 42(1), 69-73, Feb. 2001, Refereed

【論43】 Nuclear transfer in cattle: effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes
S. Hochi, M. Kato, K. Ito, M. Hirabayashi, M. Ueda, A. Sekimoto, Y. Nagao, K. Kimura, A. Hanada
J. Vet. Med. Sci., 62(10), 1111-1113, Oct. 2000, Refereed

【論44】 Production of transgenic rabbits using centrifuged pronuclear zygotes
M. Hirabayashi, M. Hirao, R. Takahashi, K. Kimura, K. Hirasawa, M. Ueda, S. Hochi
J. Vet. Med. Sci., 62(10), 1047-1052, Oct. 2000, Refereed

【論41】 Effect of time interval between biopsy and vitrification on survival of in vitro-produced bovine blastocysts
K. Ito, A. Sekimoto, M. Hirabayashi, S. Hochi, K. Kimura, M. Ueda, Y. Nagao
J. Reprod. Dev., 45(5), 351-355, Oct. 1999, Refereed

【論40】 Effects of timing of oocyte cryopreservation on in vitro development of nuclear-transferred bovine zygotes
K. Ito, M. Hirabayashi, M. Ueda, Y. Nagao, K. Kimura, A. Hanada, S. Hochi
Mol. Reprod. Dev., 54(1), 81-85, Sep. 1999, Refereed

【論39】 Effect of linoleic acid-albumin in the culture medium on freezing sensitivity of in vitro-produced bovine morulae
S. Hochi, K. Kimura, A. Hanada
Theriogenology, 52(3), 497-504, Aug. 1999, Refereed

【論38】 Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes
S. Hochi, A. Kanamori, K. Sugisawa, K. Kimura, A. Hanada
J. Mammal. Ova Res., 16(1), 19-22, Apr. 1999, Refereed

【論37】 Effects of percoll washing and glycosaminoglycans on the lysophosphatidylcholin induced acrosomal reaction in stallion
M. Kobayashi, M.P.B. Wijayagunawardane, Y.H. Choi, A.Y. Kojima, S. Hochi, N. Oguri, M. Takagi, K. Miyazawa, K. Sato
J. Equine Sci., 9(4), 101-106, Dec. 1998, Refereed

【論36】 Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage
K. Ito, S. Otake, M. Hirabayashi, S. Hochi, M. Ueda
Theriogenology, 50(7), 1093-1100, Nov. 1998, Refereed

【論35】 Effect of nuclear stages during IVM on the survival of vitrified-warmed bovine oocytes
S. Hochi, K. Ito, M. Hirabayashi, M. Ueda, K. Kimura, A. Hanada
Theriogenology, 49(4), 787-796, Mar. 1998, Refereed

【論33】 In vitro fertilizing ability of bovine oocytes frozen-thawed at immature, maturing, and mature stages
S. Hochi, A. Kanamori, K. Kimura, A. Hanada
J. Mammal. Ova Res., 14(1), 61-65, Apr. 1997, Refereed

【論32】 Direct transfer of equine blastocysts frozen-thawed in the presence of ethylene glycol and sucrose
S. Hochi, K. Maruyama, N. Oguri
Theriogenology, 46(7), 1217-1224, Nov. 1996, Refereed

【論31】 Effect of cooling and warming rates during cryopreservation on survival of in vitro-produced bovine embryos
S. Hochi, E. Semple, S.P. Leibo
Theriogenology, 46(5), 837-847, Oct. 1996, Refereed

【論29】 In vitro capacitation of stallion spermatozoa assessed by the lysophoshatidylcholine-induced acrosome reaction and the penetration rate into in vitro-matured, zona-free mare oocytes
S. Hochi, K. Korosue, Y.H. Choi, N. Oguri
J. Equine Vet. Sci., 16(6), 244-248, Jun. 1996, Refereed

【論30】 In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification
S. Hochi, M. Kozawa, T. Fujimoto, E. Hondo, J. Yamada, N. Oguri
Cryobiology, 33(3), 300-310, Jun. 1996, Refereed

【論28】 Activation of glutathione transferase P gene by lead requires glutathione transferase P enhancer I
T. Suzuki, S. Morimura, M.B. Diccianni, R. Yamada, S. Hochi, M. Hirabayashi, A. Yuki, K. Nomura, T. Kitagawa, M. Imagawa, M. Muramatsu
J. Biol. Chem., 271(3), 1626-1632, Jan. 1996, Refereed

【論26】 Effect of glucose in the culture medium on development of horse oocytes matured and microfertilized in vitro
T. Azuma, Y.H. Choi, S. Hochi, N. Oguri
Reprod. Fertil. Dev., 7(5), 1067-1071, Oct. 1995, Refereed

【論27】 Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa
J. Braun, S. Hochi, N. Oguri, K. Sato, F. Torres-Boggino
Cryobiology, 32(5), 487-492, Oct. 1995, Refereed

【論25】 Tissue specific activation of tumor marker glutathione transferase P transgenes in transgenic rats
T. Suzuki, M. Imagawa, K. Nomura, S. Hochi, M. Hirabayashi, M. Ueda, T. Kitagawa, M. Muramatsu
J. Can. Res. Clin. Oncol., 121(9-10), 606-611, Sep. 1995, Refereed

【論24】 An attempt to produce acellular embryonic capsule in equine blastocysts cultured in vitro
S. Hochi, T. Azuma, K. Miyaushiro, N. Oguri
J. Reprod. Dev., 41(4), 367-371, Aug. 1995, Refereed

【論23】 Relationship among seminal characteristics, fertility, and suitability for semen preservation in Draft stallions
F. Torres-Boggino, K. Sato, A. Oka, Y. Kanno, S. Hochi, N. Oguri, J. Braun
J. Vet. Med. Sci., 57(2), 225-229, Apr. 1995, Refereed

【論22】 Large equine blastocysts are damaged by vitrification procedures
S. Hochi, T. Fujimoto, N. Oguri
Reprod. Fertil. Dev., 7(1), 113-117, Feb. 1995, Refereed

【論21】 Cryopreservation of equine oocytes by 2-step freezing
S. Hochi, T. Fujimoto, Y.H. Choi, J. Braun, N. Oguri
Theriogenology, 42(7), 1085-1094, Nov. 1994, Refereed

【論20】 In vitro fertilization rate of horse oocytes with partially removed zonae
Y.H. Choi, Y. Okada, S. Hochi, J. Braun, K. Sato,N. Oguri
Theriogenology, 42(5), 795-802, Oct. 1994, Refereed

【論18】 Effects of individual stallions and mare breeds on assisted fertilization in vitro
S. Hochi, Y.H. Choi, K. Korosue, K. Sato, N. Oguri
J. Equine Sci., 5(3), 77-81, Sep. 1994, Refereed

【論16】 Effect of seminal plasma on motion characteristics of epididymal and ejaculated stallion spermatozoa during storage at 5˚C
J. Braun, F. Torres-Boggino, S. Hochi, N. Oguri
Deutsche Tierärztliche Wochenschrift, 101(8), 319-322, Aug. 1994, Refereed

【論17】 Pregnancies following transfer of equine embryos cryopreserved by vitrification
S. Hochi, T. Fujimoto, J. Braun, N. Oguri
Theriogenology, 42(3), 483-488, Aug. 1994, Refereed

【論15】 Influence of relative embryonic volumes during glycerol equilibration on the survival of frozen-thawed equine blastocysts
S. Hochi, M. Ogasawara, J. Braun, N. Oguri
J. Reprod. Dev., 40(3), 243-249, Jun. 1994, Refereed

【論14】 Follicular development, progesterone and estradiol 17-β levels during spontaneous and FSH-stimulated estrous cycles in Hokkaido Native pony and Thoroughbred mares
J. Braun, Y. Moriuchi, S. Nakagawa, S. Hochi, A. Miyamoto, N. Oguri
J. Reprod. Dev., 40(2), 141-147, Apr. 1994, Refereed

【論13】 Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezing
J. Braun, M. Sakai, S. Hochi, N. Oguri
Theriogenology, 41(4), 809-818, Mar. 1994, Refereed

【論12】 Influence of sera on in vitro hatching of equine blastocysts
S. Hochi, J. Braun, A. Miyamoto, Y. Fukui, N. Oguri
J. Reprod. Dev., 40(1), 13-18, Feb. 1994, Refereed

【論9】 In vitro maturation of equine oocytes collected by follicle aspiration and by the slicing of ovaries
Y.H. Choi, S. Hochi, J. Braun, K. Sato, N. Oguri
Theriogenology, 40(5), 959-966, Nov. 1993, Refereed

【論8】 The hatching process of equine embryos in vitro
S. Hochi, J. Braun, N. Oguri
Equine Vet. J. Suppl., 15, 91-94, Oct. 1993, Refereed

【論7】 Trans-activation of glutathione transferase P gene during chemical hepatocarcinogenesis of the rat
S. Morimura, T. Suzuki, S. Hochi, A. Yuki, K. Nomura, T. Kitagawa, I. Nagatsu, M. Imagawa, M. Muramatsu
Proc. Natl. Acad. Sci. USA, 90(5), 2065-2068, Mar. 1993, Refereed

【論6】 Secretion of bovine α-lactalbumin into the milk of transgenic rats
S. Hochi, T. Ninomiya, M. Waga-Honma, J. Sagara, A. Yuki
Mol. Reprod. Dev., 33(2), 160-164, Oct. 1992, Refereed

【論5】 Cryoprotective effect of polyols on rat embryos during two-step freezing
K. Utsumi, S. Hochi, A. Iritani
Cryobiology, 29(3), 332-341, Jun. 1992, Refereed

【論4】 Successful production of transgenic rats
S. Hochi, T. Ninomiya, M. Honma, A. Yuki
Anim. Biotechnol., 1(2), 175-184, Dec. 1990, Refereed

【論3】 DNA breaks and repair in the mouse leukemia L1210 cells exposed to three types of interstrand DNA cross-linkers
N. Yajima, S. Hochi, N. Miyata, T. Kishi, G. Kawanishi
Mutat. Res., 236(1), 43-50, Jul. 1990, Refereed

【論2】 Fate of exogenous DNA carried into mouse eggs by spermatozoa
S. Hochi, T. Ninomiya, A. Mizuno, M. Honma, A. Yuki
Anim. Biotechnol., 1(1), 21-31, Jun. 1990, Refereed

Cryodevices developed for minimum volume cooling vitrification of bovine oocytes
S. Hochi
Anim. Sci. J., 93(1), e13683, Jan. 2022, Refereed, Invited
Lead, Last, Corresponding

Pluripotent stem cell-derived organogenesis in the rat model system
M. Hirabayashi, T. Goto, S. Hochi
Transgenic Res., 28(3-4), 287-297, Aug. 2019, Refereed
Last

Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies
S. Hochi
Anim. Sci. J., 87(9), 1076-1083, Sep. 2016, Refereed
Lead, Last, Corresponding

Recent progress in cryopreservation of bovine oocytes
I-S. Hwang, S. Hochi
BioMed. Res. Intern., 2014, 570647, Mar. 2014, Refereed
Last, Corresponding

Challenging endeavour for preservation of freeze-dried mammalian spermatozoa
S. Hochi, H. Abdalla, H. Hara, M. Hirabayashi
J. Reprod. Dev., 57(5), 557-563, Oct. 2011, Refereed, Invited
Lead, Corresponding

【総説12】 Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in rats
M. Hirabayashi, S. Hochi
Reprod. Med. Biol., 10(4), 231-238, Jun. 2011, Refereed

【総説11】 Active demethylation of paternal genome in mammalian zygotes
H. Abdalla, Y. Yoshizawa, S. Hochi
J. Reprod. Dev., 55(4), 356-360, Aug. 2009, Refereed, Invited
Last, Corresponding

【総説9】 Transgenesis via intracytoplasmic sperm injection (ICSI) in rodents
M. Hirabayashi, M. Kato, S. Hochi
J. Mammal. Ova Res., 23(3), 86-90, Oct. 2006, Refereed

【総説6】 Cryopreservation of follicular oocytes and preimplantation embryos in cattle and horses
S. Hochi
J. Reprod. Dev., 49(1), 13-21, Feb. 2003, Refereed

【総説5】 Maintenance of transgenes in rats: the contributions of embryo cryopreservation and intracytoplasmic sperm/spermatid injection
S. Hochi, M. Hirabayashi
J. Reprod. Dev., 48(3), 205-213, Jun. 2002, Refereed

【総説2】 Current status of IVM/IVF/IVC technology and embryo cryopreservation in the equine species
S. Hochi
J. Mammal. Ova Res., 15(3), 117-127, Oct. 1998, Refereed

Silk fibroin sponge as a cryodevice combined with transporter and scaffold functions in cellular transplantation
S. Hochi, Y. Tamada, Joint work
Nova Science Publishers, "Silk Fibroin: Advances in Applications and Research (Ed. S.H. Pawar)", 103-115, Jan. 2023
ISBN:979-8-88697-402-7

Organ generation from knocked-out rat blastocysts complemented with pluripotent stem cells (Chapter 18)
M. Hirabayashi, S. Hochi, Joint work
Humana Press, "Microinjection (Eds. C. Liu and Y. Du) / Methods in Molecular Biology 1874", 313-326, Nov. 2019
ISBN:978-1-4939-8831-0

Embryonic stem cells and gene modification in rat (Chapter 5)
M. Hirabayashi, A. Takizawa, S. Hochi, Joint work
Humana Press, "Rat Genomics (Eds. G.T. Hayman, J.R. Smith, M.R. Dwinell and M. Shimoyama) / Methods in Molecular Biology 2018", 115-130, Jun. 2019
ISBN:978-1-4939-9581-3

Intracytoplasmic sperm injection in cattle (Chapter 2)
S. Hochi, H. Abdalla, M. Hirabayashi, Joint work
Nova Science Publishers, "In Vitro Fertilization (Ed. S.P. Steiger)", 35-63, Oct. 2011
ISBN:978-1612099613

Rat embryonic stem cells: establishement and their use for transgenesis (Chapter 22)
M. Hirabayashi, S. Hochi, Joint work
InTech, "Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Application (Ed. C. Atwood)", 397-410, Apr. 2011
ISBN:978-953-307-197-8

【著7】 Generation of transgenic rats by ooplasmic injection of sperm cells exposed to exogenous DNA (Chapter 9)
M. Hirabayashi, S. Hochi, Joint work
Humana Press, "Rat Genomics (Ed. I. Anegon) / Methods in Molecular Biology 597", 127-136, Dec. 2009
ISBN:978-1-60327-389-3_9

【著6】 Cloning in the rat (Chapter 10)
M. Hirabayashi, S. Hochi, Joint work
Taylar and Francis Books, "Epigenetic Risks of Cloning (Ed. A. Inui)", 165-175, Jul. 2005
ISBN:0-8493-2527-7

【著3】 Cryopreservation of in vitro-produced bovine embryos (Section 7)
S. Hochi, Single work
Hokuto Shobo, "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)", 333-339, 2001
ISBN:4-89467-056-9

【著4】 Systematic production of transgenic rats by pronuclear DNA microinjection (Section 6)
M. Hirabayashi, S. Hochi, Joint work
Hokuto Shobo, "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)", 243-250, 2001
ISBN:4-89467-056-9

【著2】 Cryopreservation of preimplantation embryos and follicular oocytes in cattle and horses (Section 6)
S. Hochi, Single work
Shoukadoh Booksellers, "Reproductive Biology Update (Eds. H. Miyamoto and N. Manabe)", 425-438, 1998
ISBN:4-87974-981-8

Scientific English
Shinshu University

Topic in Animal Reproductive Technology (Doctor course)
Shinshu University

Topic in Animal Science (Master course)
Shinshu University

Cell Technology
Shinshu University

Animal Physiology
Shinshu University

Safety Education
Shinshu University

Invitation to Applied Biology
Shinshu University

Biology World
Shinshu University

1990 - 2025
Society for Reproduction and Development (SRD)

1990 - 2023
Japanese Society of Animal Science (JSAS)

1990 - 2020
Japanese Society for Mammalian Ova Research (JSMOR)

Rat model study for optimal processing of donor pancreatic islets for euglycemic control by subrenal transplantation
Grant-in-Aid for Scientific Research, Japan Society for the Promotion of Science, (C)
2024 - 2026

Novel cryodevices for vitrification of cumulus-oocyte complexes and pancreatic islets
Grant-in-Aid for Scientific Research, Japan Society for the Promotion of Science, (C)
2020 - 2023

Cryopreservation of complex structures comprised from different cell types (bovine cumulus-oocyte complexes and rat pancreatic islets)
Grant-in-Aid for Scientific Research, Japan Society for the Promotion of Science, (C)
2016 - 2019

System for bovine blastocyst production from lyophilized sperm and diploid cells
Grant-in-Aid for Scientific Research, Japan Society for the Promotion of Science, (C)
2012 - 2015

Evaluation of freeze-dried bull spermatozoa by intra- or inter-species microinsemination
Grant-in-Aid for Scientific Research, Japan Society for the Promotion of Science, (C)
2008 - 2011

Cryopreservation of pronuclear-stage zygotes for transgenic rabbit production
Grant-in-Aid for Scientific Research, (C)
2001 - 2002

Chairperson
Planning etc
The 111th Annual Meeting, The Society for Reproduction and Development, Shinshu University, Ueda, Nagano, Japan, 12 Sep. 2018 - 16 Sep. 2018

Peer review
Peer review
Theriogenology (75) / Reprod. Domest. Anim. (21) / J. Reprod. Dev. (17) / Cryobiology (16) / J. Assist. Reprod. Genet. (11) / Reproduction (6) / Zygote (5) etc. Total 43 journals, 235 manuscripts, Jan. 1998 - Feb. 2025