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Academic OrganizationAcademic Assembly School of Science and Technology Institute of Textile Science and TechnologyTEL0268-21-5350
Education and Research OrganizationFaculty of Textile Science and Technology Department of Applied BiologyFAX0268-21-5830
Address3-15-1 Tokida, Ueda, Nagano 386-8567Web site


Assigned Class
Graduate School of Science and Technology
Interdisciplinary Graduate School of Medicine, Science and Technology
Research Field
Keywords:Embryos , Spermatozoa , Oocytes , Pancreatic islets , Cryopreservation , Transplantation
Current Subject
Advanced Reproductive Technology in Mammals
Keywords:Freeze-drying: Transgenic animals: Microinsemination , 1996-
Academic Societies
Academic Societies
Society for Reproduction and Development (SRD)
Japanese Society of Animal Science (JSAS)

Committee of Academic Societies
2019-2022 , Society for Reproduction and Development (SRD) , Governor
2018-2018 , The 111th SRD meeting , President
2009-2018 , Society for Reproduction and Development (SRD) , Board
2007-2018 , Japanese Society for Ova Research (JSOR) , Board
Academic Background
Graduate School
Graduate School, Okayama University , (Animal Science , Division of Agriculture) , 1986

Okayama University , (Faculty of Agriculture) , 1984

Doctor of Agriculture , Kyoto University, 1991
2006 , Japanese Association for Experimental Animal Technologists: 25th Award for Research Encouragement
2002 , Japanese Society for Animal Reproduction: Award for Outstanding Research


Books, Articles, etc.
Silk fibroin sponge as a cryodevice combined with transporter and scaffold functions in cellular transplantation
Nova Science Publishers, "Silk Fibroin: Advances in Applications and Research (Ed. S.H. Pawar)", 103-115 2023(Jan.)
Author:S. Hochi, Y. Tamada

【著11】 Organ generation from knocked-out rat blastocysts complemented with pluripotent stem cells (Chapter 18)
Humana Press, "Microinjection (Eds. C. Liu and Y. Du) / Methods in Molecular Biology 1874", 313-326 2019(Nov.)
Author:M. Hirabayashi, S. Hochi

【著10】 Embryonic stem cells and gene modification in rat (Chapter 5)
Humana Press, "Rat Genomics (Eds. G.T. Hayman, J.R. Smith, M.R. Dwinell and M. Shimoyama) / Methods in Molecular Biology 2018", 115-130 2019(Jun.)
Author:M. Hirabayashi, A. Takizawa, S. Hochi

【著9】 Intracytoplasmic sperm injection in cattle (Chapter 2)
Nova Science Publishers, "In Vitro Fertilization (Ed. S.P. Steiger)", 35-63 2011(Oct.)
Author:S. Hochi, H. Abdalla, M. Hirabayashi

【著8】 Rat embryonic stem cells: establishement and their use for transgenesis (Chapter 22)
InTech, "Methodological Advances in the Culture, Manipulation and Utilization of Embryonic Stem Cells for Basic and Practical Application (Ed. C. Atwood)", 397-410 2011(Apr.)
Author:M. Hirabayashi, S. Hochi

【著7】 Generation of transgenic rats by ooplasmic injection of sperm cells exposed to exogenous DNA (Chapter 9)
Humana Press, "Rat Genomics (Ed. I. Anegon) / Methods in Molecular Biology 597", 127-136 2009(Dec.)
Author:M. Hirabayashi, S. Hochi

【著6】 Cloning in the rat (Chapter 10)
Taylar and Francis Books, "Epigenetic Risks of Cloning (Ed. A. Inui)", 165-175 2005(Jul.)
Author:M. Hirabayashi, S. Hochi

【著4】 Systematic production of transgenic rats by pronuclear DNA microinjection (Section 6)
Hokuto Shobo, "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)", 243-250 2001
Author:M. Hirabayashi, S. Hochi

【著3】 Cryopreservation of in vitro-produced bovine embryos (Section 7)
Hokuto Shobo, "Reproductive Biotechnology (Eds. H. Miyamoto and N. Manabe)", 333-339 2001
Author:S. Hochi

【著2】 Cryopreservation of preimplantation embryos and follicular oocytes in cattle and horses (Section 6)
Shoukadoh Booksellers, "Reproductive Biology Update (Eds. H. Miyamoto and N. Manabe)", 425-438 1998
Author:S. Hochi

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos
Front. Cell Dev. Biol.,11:1238546 2023(Oct.)
Author:W-J. Jiang, S-H. Lee, G. Heo, H-J. Chung, E-S. Cho, S-J. Sa, S. Hochi, X-S. Cui

Rat post-implantation epiblast-derived pluripotent stem cells produce functional germ cells
Cell Rep. Methods,3(8):100542 2023(Aug.)
Author:K. Iwatsuki, M. Oikawa, H. Kobayashi, C.A. Penfold, M. Sanbo, T. Yamamoto, S. Hochi, K. Kurimoto, M. Hirabayashi, T. Kobayashi

Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using ProteinChip CM10
J. Anim. Sci. Technol.,65(2):401-411 2023(Mar.)
Author:S-W. Park, Y-M. Kim, M-J. Lee, D-Y. Jeon, S-J. Sa, Y-D. Jeong, H-S. Seong, S. Hochi, E-S. Cho, H-J. Chung

Characterization of homozygous Foxn1 mutations induced in rat embryos by different delivery forms of Cas9 nuclease
Mol. Biol. Rep.,50(2):1231-1239 2023(Feb.)
Author:T. Goto, K. Yogo, S. Hochi, M. Hirabayashi

High survival of bovine mature oocytes after nylon mesh vitrification, as assessed by intracytoplasmic sperm injection
J. Reprod. Dev.,68(5):335-339 2022(Aug.)
Author:S. Hochi, M. Ide, S. Ueno, M. Hirabayashi

Fabrication of functional rat pseudo-islets after cryopreservation of pancreatic islets or dispersed islet cells
J. Tissue Eng. Regen. Med.,15(7):686-696 2021(May)
Author:K. Nakayama-Iwatsuki, M. Hirabayashi, S. Hochi

All-in-one silk fibroin sponge as the vitrification cryodevice of rat pancreatic islets and the VEGF-embedded scaffold for subrenal transplantation
Transplant. Proc.,53(5):1744-1750 2021(Apr.)
Author:T. Yamanaka, K. Nakayama-Iwatsuki, S. Fujimoto, N. Hirono, J. Negishi, Y. Tamada, M. Hirabayashi, S. Hochi

Acellular matrix derived from rat liver improves the functionality of rat pancreatic islets before and after vitrification
Cryobiology,100:90-95 2021(Mar.)
Author:K. Nakayama-Iwatsuki, K. Yanagisawa, D. Tanaka, M. Hirabayashi, J. Negishi, S. Hochi

Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats
Nat. Commun.,12(1):1328 2021(Feb.)
Author:T. Kobayashi, T. Goto, M. Oikawa, M. Sanbo, F. Yoshida, R. Terada, N. Niizeki, N. Kajitani, K. Kazuki, Y. Kazuki, S. Hochi, H. Nakauchi, M.A. Surani, M. Hirabayashi

Altrenogest affects expression of galectin-3 and fibroblast growth factor 9 in the reproductive tract of sows
Anim. Biotechnol.,32(5):537-543 2021(Feb.)
Author:S-Y. Baek, S-J. Sa, Y-D. Jeong, E-S. Cho, J-G. Hong, Y-S. Kim, K-H. Cho, S-H. Park, K-W. Kim, H-C. Lee, S. Hochi, S-H. Lee, I-C. Choi, H-J. Chung

Transplantation of rat pancreatic islets vitrified-warmed on the nylon mesh device and the silk fibroin sponge disc
Islets,12(6):145-155 2020(Dec.)
Author:K. Nakayama-Iwatsuki, T. Yamanaka, J. Negishi, J. Teshima, Y. Tamada, M. Hirabayashi, S. Hochi

Rescue of vitrified-warmed bovine mature oocytes by short-term recovery culture with resveratrol
Cryobiology,97:185-190 2020(Dec.)
Author:S. Chinen, T. Yamanaka, M. Hirabayashi, S. Hochi

Silk fibroin sheet multilayer suitable for vitrification of in vitro-matured bovine oocytes
Theriogenology,145:109-114 2020(Mar.)
Author:K. Nakayama, S. Chinen, J. Teshima, Y. Tamada, M. Hirabayashi, S. Hochi

【論119】 Nylon mesh cryodevice for bovine mature oocytes, easily removable excess vitrification solution
Cryobiology,90:96-99 2019(Oct.)
Author:S. Chinen, T. Yamanaka, K. Nakayama, H. Watanabe, Y. Akiyama, M. Hirabayashi, S. Hochi

【論118】 Production of human tissue-type plasminogen activator (htPA) using in vitro cultured transgenic pig mammary gland cells
Anim. Biotechnol.,30(4):317-322 2019(Aug.)
Author:H-J. Chung, H-J. Park, S-Y. Baek, J-K. Park, W-Y. Lee, K-W. Kim, Y-M. Jo, S. Hochi, Y-M. Kim, T-J. Choi, E-S. Cho, K-H. Cho

【論117】 Supplementary cryoprotective effect of carboxylated ε-poly-L-lysine during vitrification of rat pancreatic islets
Cryobiology,88:70-74 2019(Jun.)
Author:K. Nakayama, T. Yamanaka, Y. Tamada, M. Hirabayashi, S. Hochi

【論116】 Generation of pluripotent stem cell-derived mouse kidneys in Sall1-targeted anephric rats
Nat. Commun.,10(1):451 2019(Feb.)
Author:T. Goto, H. Hara, M. Sanbo, H. Masaki, H. Sato, T. Yamaguchi, S. Hochi, T. Kobayashi, H. Nakauchi, M. Hirabayashi

【論115】 FGF2 has distinct molecular functions from GDNF in the mouse germline niche
Stem Cell Rep.,10(6):1782-1792 2018(Jun.)
Author:K. Masaki, M. Sakai, S. Kuroki, J. Jo, K. Hoshina, Y. Fujimori, K. Oka, T. Amano, T. Yamanaka, M. Tachibana, Y. Tabata, T. Shinozawa, O. Ishizuka, S. Hochi, S. Takashima

【論114】 Haploid embryonic stem cell lines derived from andorogenetic and parthenogenetic rat blastocysts
J. Reprod. Dev.,63(6):611-616 2017(Dec.)
Author:M. Hirabayashi, H. Hara, T. Goto, A. Takizawa, M.R. Dwinell, T. Yamanaka, S. Hochi, H. Nakauchi

【論113】 Nylon mesh device for vitrification of large quantities of rat pancreatic islets
Biopreserv. Biobank.,15(5):457-462 2017(Oct.)
Author:T. Yamanaka, T. Goto, M. Hirabayashi, S. Hochi

【論112】 Downsizing cumulus cell layers to improve cryotolerance of germinal vesicle-stage bovine oocytes
Theriogenology,95:1-7 2017(Jun.)
Author:K. Tashima, Y. Kubo, M. Hirabayashi, S. Hochi

【論111】 Direct comparison of Cryotop® vitrification and Bicell® freezing on recovery of functional rat pancreatic islets
Cryobiology,73(3):376-382 2016(Dec.)
Author:T. Yamanaka, K. Tashima, R. Takahashi, S. Takashima, T. Goto, M. Hirabayashi, S. Hochi

【論110】 Hypomorphic phenotype of Foxn1 gene-modified rats by CRISPR/Cas9 system
Transgenic Res.,25(4):533-544 2016(Aug.)
Author:T. Goto, H. Hara, H. Nakauchi, S. Hochi, M. Hirabayashi

【論109】 Rat blastocysts from nuclear injection and time-lagged enucleation and their commitment to embryonic stem cells
Cell. Reprogram.,18(2):108-115 2016(Apr.)
Author:H. Hara, T. Goto, A. Takizawa, H.J. Jacob, M. Sanbo, T. Kobayashi, H. Nakauchi, S. Hochi, M. Hirabayashi

【論108】 Multiple aster formation is frequently observed in bovine oocytes retrieved from 1 day-stored ovaries
Zygote,24(1):115-120 2016(Feb.)
Author:H. Hara, M. Tagiri, M. Hirabayashi, S. Hochi

【論107】 High incidence of polyspermic fertilization in bovine oocytes matured in vitro after Cryotop vitrification
Cryo Letters,37(1):27-33 2016(Jan.)
Author:I-S. Hwang, D-J. Kwon, G-S. Im, K. Tashima, S. Hochi, S. Hwang

【論106】 Knock-in of a histone H2B-tdTomato reporter into the Rosa26 locus allows visualization of cell nuclei in rats
Mol. Reprod. Dev.,82(12):916-917 2015(Dec.)
Author:T. Goto, T. Kobayashi, H. Hara, M. Sanbo, S. Hochi, H. Nakauchi, M. Hirabayashi

【論105】 High revivability of vitrified-warmed bovine oocytes during recovery culture with α-tocopherol
Reproduction,149(4):347-355 2015(Apr.)
Author:I. Yashiro, M. Tagiri, H. Ogawa, K. Tashima, S. Takashima, H. Hara, M. Hirabayashi, S. Hochi

【論104】 Microtubule assembly and in vitro development of bovine oocytes with increased intracellular glutathione level prior to vitrification and in vitro fertilization
Zygote,22(4):476-482 2014(Nov.)
Author:H. Hara, I. Yamane, I. Noto, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi

【論103】 Adverse effect of cake collapse on the functional integrity of freeze-dried bull spermatozoa
Cryobiology,68(3):354-360 2014(Jun.)
Author:H. Hara, M. Tagiri, I-S. Hwang, M. Takahashi, M. Hirabayashi, S. Hochi

【論102】 Effect of leukemia inhibitory factor and forskolin on establishment of rat embryonic stem cell lines
J. Reprod. Dev.,60(1):78-82 2014(Mar.)
Author:M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, S. Hochi

【論101】 Derivation of embryonic stem cell lines from parthenogenetically developing rat blastocysts
Stem Cells Dev.,23(2):107-114 2014(Jan.)
Author:M. Hirabayashi, T. Goto, C. Tamura, M. Sanbo, H. Hara, M. Kato-Itoh, H. Sato, T. Kobayashi, H. Nakauchi, S. Hochi

【論100】 Rescue of vitrified-warmed bovine oocytes with Rho-associated coiled-coil kinase inhibitor
Biol. Reprod.,89(2):26 2013(Aug.)
Author:I-S. Hwang, H. Hara, H-J. Chung, M. Hirabayashi, S. Hochi

【論99】 A retrospective analysis of germline competence in rat embryonic stem cell lines
Transgenic Res.,22(2):411-416 2013(Apr.)
Author:M. Hirabayashi, C. Tamura, M. Sanbo, M. Kato-Itoh, T. Kobayashi, H. Nakauchi, S. Hochi

【論98】 Proteins associated with reproductive disorders in testes of human erythropoietin gene-harboring transgenic boars
Theriogenology,78(5):1020-1029 2012(Sep.)
Author:M-S. Choi, M-R. Shim, M-Y. Oh, K-W. Kim, H-C. Lee, B-C. Yang, H-K. Chung, J-H. Kim, H-T. Lee, I-S. Hwang, S. Hochi, Y-T. Heo, N-H. Kim, S-J. Uhm, J-K. Park, W-K.Chang, H-J. Chung

【論97】 Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells
Mol. Reprod. Dev.,79(6):402-412 2012(Jun.)
Author:M. Hirabayashi, C. Tamura, M. Sanbo, T. Goto, M. Kato, T. Kobayashi, H. Nakauchi, S. Hochi

【論96】 High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization
Theriogenology,77(5):908-915 2012(Mar.)
Author:H. Hara, I-S. Hwang, N. Kagawa, M. Kuwayama, M. Hirabayashi, S. Hochi

【論95】 Birefringent parameter available for quantitative analysis of human zona hardness
Zygote,19(4):323-329 2011(Nov.)
Author:H. Iwayama, S. Hochi, M. Yamashita

【論94】 Procedure for bovine ICSI, not sperm freeze-drying, impairs the function of the microtubule-organizing center
J. Reprod. Dev.,57(3):428-432 2011(Jun.)
Author:H. Hara, H. Abdalla, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi

【論93】 In vitro and in vivo viability of human blastocysts collapsed by laser pulse or osmotic shock prior to vitrification
J. Assist. Reprod. Genet.,28(4):355-361 2011(Apr.)
Author:H. Iwayama, S. Hochi, M. Yamashita

【論92】 Low stretching ability of human ooplasma during piezo-ICSI as a risk factor on post-injection survival and implantation
J. Mammal. Ova Res.,27(3):150-156 2010(Oct.)
Author:H. Iwayama, S. Hochi, M. Yamashita

【論91】 Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
Theriogenology,74(6):1028-1035 2010(Oct.)
Author:H. Abdalla, M. Shimoda, H. Hara, H. Morita, M. Kuwayama, M. Hirabayashi, S. Hochi

【論90】 Rat transgenesis via embryonic stem cells electroporated with Kusabira-orange gene
Mol. Reprod. Dev.,77(6):474 2010(Jun.)
Author:M. Hirabayashi, M. Kato, M. Sanbo, T. Kobayashi,S. Hochi, H. Nakauchi

【論89】 Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
Theriogenology,73(8):1139-1145 2010(May)
Author:S. Hochi, H. Abdalla, M. Shimoda, H. Hara, H. Morita, M, Kuwayama, M. Hirabayashi

【論88】 Establishment of rat embryonic stem cell lines that can participate into germline chimerae at high efficiency
Mol. Reprod. Dev.,77(2):94 2010(Feb.)
Author:M. Hirabayashi, M. Kato, T. Kobayashi, M. Sanbo, T. Yagi, S. Hochi, H. Nakauchi

【論87】 Impaired active demethylation of paternal genome in pronuclear-stage rat zygotes produced by in vitro ferilization or intracytoplasmic sperm injection
Mol. Reprod. Dev.,77(1):69-75 2010(Jan.)
Author:Y. Yoshizawa, M. Kato, M. Hirabayashi, S. Hochi

【論86】 A combined treatment of ionomycin with ethanol improves blastocyst development of bovine oocytes harvested from stored ovaries and microinjected with spermatozoa
Theriogenology,72(4):453-460 2009(Sep.)
Author:H. Abdalla, M. Shimoda, M. Hirabayashi, S. Hochi

【論85】 Demethylation dynamics of paternal genome in pronuclear-stage bovine zygotes produced by in vitro fertilization and ooplasmic injection of freeze-thawed or freeze-dried spermatozoa
J. Reprod. Dev.,55(4):433-439 2009(Aug.)
Author:H. Abdalla, M. Hirabayashi, S. Hochi

【論84】 Activation regimens for full-term development of rabbit oocytes injected with round spermatids
Mol. Reprod. Dev.,76(6):573-579 2009(Jun.)
Author:M. Hirabayashi, M. Kato, K. Kitada, N. Ohnami, M. Hirao, S. Hochi

【論83】 Availability of subfertile transgenic rats expressing c-myc gene as recipients for spermatogonial transplantation
Transgenic Res.,18(1):135-141 2009(Feb.)
Author:M. Hirabayashi, Y. Yoshizawa, M. Kato, T. Tsuchiya, S. Nagao, S. Hochi

【論82】 The ability of freeze-dried bull spermatozoa to induce calcium oscillations and resumption of meiosis
Theriogenology,71(3):543-552 2009(Feb.)
Author:H. Abdalla, M. Hirabayashi, S. Hochi

【論81】 Factors affecting full-term development of rat oocytes microinjected with fresh or cryopreserved round spermatids
Exp. Anim.,57(4):401-405 2008(Jul.)
Author:M. Hirabayashi, M. Kato, S. Hochi

【論80】 Effect of post-IVF developmental kinetics on in-vitro survival of vitrified-warmed domestic cat blastocysts
Reprod. Domest. Anim.,43(3):323-327 2008(Jun.)
Author:T.Tsujioka, C. Otzdorff, J. Braun, S. Hochi

【論79】 Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year
Mol. Reprod. Dev.,75(5):890-894 2008(May)
Author:S. Hochi, K. Watanabe, M. Kato, M. Hirabayashi

【論78】 Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats
Exp. Anim.,57(2):145-148 2008(Apr.)
Author:M. Hirabayashi, M. Kato, K. Amemiya, S. Hochi

【論77】 Follicle size-dependent changes of follicular fluid components and oocyte diameter in Antarctic minke whales (Balaenoptera bonaerensis)
J. Reprod. Dev.,53(6):1265-1272 2007(Dec.)
Author:H. Nagai, T. Mogoe, H. Ishikawa, S. Hochi, S. Ohsumi, Y. Fukui

【論76】 Attempt at intracytoplasmic sperm injection to in vitro matured oocytes in common minke whales (Balaenoptera acutorostrata) captured at Kushiro coast survey
J. Reprod. Dev.,53(4):945-952 2007(Aug.)
Author:Y. Fukui, H. Iwayama, T. Matsuoka, H. Nagai, N. Koma, T. Mogoe, H. Ishikawa, Y.Fujise, M. Hirabayashi, S. Hochi, H. Kato, S. Ohsumi

【論75】 Effect of enucleation on inactivation of cytostatic factor activity in matured rat oocytes
Cloning Stem Cells,9(2):257-266 2007(Jul.)
Author:J. Ito, M. Kato, S. Hochi, M. Hirabayashi

【論74】 The ability of whale haploid spermatogenic cells for inducing calcium oscillations and its relevance to oocyte activation
Zygote,15(2):103-108 2007(May)
Author:K. Amemiya, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi

【論73】 Conventional freezing of in vitro produced and biopsied bovine blastocysts in the presence of low concentration glycerol with sucrose
J. Reprod. Dev.,53(2):443-447 2007(Apr.)
Author:K. Tominaga, F. Iwaki, S. Hochi

【論72】 Quality analysis of buffalo blastocysts derived from oocytes vitrified before and after enucleation and reconstructed with somatic cell nuclei
Theriogenology,67(4):893-900 2007(Mar.)
Author:S. Muenthaisong, C. Laowtammathron, M. Katudat-Cairns, R. Parnpai, S. Hochi

【論71】 Involvement of Ca(2+)-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of rat matured oocytes
Theriogenology,67(3):475-485 2007(Feb.)
Author:J. Ito, M. Shimada, S. Hochi, M. Hirabayashi

【論70】 Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection
Zygote,14(2):89-95 2006(May)
Author:T. Fujihira, M. Kobayashi, S. Hochi, M. Hirabayashi, H. Ishikawa, S. Ohsumi, Y. Fukui

【論69】 No effect of recombinase-mediated DNA transfer on production efficiency of transgenic rats
Exp. Anim.,55(2):131-135 2006(Apr.)
Author:M. Hirabayashi, M. Kato, R. Kaneko, T. Hirabayashi, M. Morira, S. Hochi

【論68】 Contribution of spermatozoal centrosomes to the microtubiale-organizing centre in Antarctic minke whale (Balaenoptera bonaerensis)
Zygote,14(1):45-51 2006(Feb.)
Author:T. Kobayashi, K. Amemiya, K. Takeuchi, T. Tsujioka, K. Tominaga, M. Hirabayashi, H. Ishikawa, Y. Fukui, S. Hochi

【論67】 Characteristics of rat round spermatids differentiated from spermatogonial cells during co-culture with Sertoli cells, assessed by flow cytometry, microinsemination and RT-PCR
Theriogenology,65(2):288-298 2006(Jan.)
Author:Y. Iwanami, T. Kobayashi, M. Kato, M. Hirabayashi, S. Hochi

【論66】 Gel-loading tip vitrification of in vitro-matured bovine oocytes and subsequent embryo production by IVF and nuclear transfer
J. Mammal. Ova Res.,22(3):178-184 2005(Oct.)
Author:K. Tominaga, Y. Hamada, S. Hochi

【論65】 Factors affecting cryosurvival of nuclear-transferred bovine and swamp buffalo blastocysts: effects of hatching stage, linoleic acid-albumin in IVC medium and Ficoll supplementation to vitrification solution
Theriogenology,64(5):1185-1196 2005(Sep.)
Author:C. Laowtammathron, C. Lorthongpanich, M. Ketudat-Cairns, S. Hochi, R. Parnpai

【論64】 Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA
Mol. Reprod. Dev.,70(4):422-428 2005(Apr.)
Author:M. Hirabayashi, M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi

【論63】 Viable rat offspring derived from oocytes intracytoplasmically injected with freeze-dried sperm heads
Zygote,13(1):79-85 2005(Feb.)
Author:M. Hirabayashi, M. Kato, J. Ito, S. Hochi

【論62】 Contribution of high p34(cdc2) kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes
Reproduction,129(2):171-180 2005(Feb.)
Author:J. Ito, M. Hirabayashi, M. Kato, A. Takeuchi, M. Ito, M. Shimada, S. Hochi

【論61】 Effects of cryodevice type and donors' sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicle-stage
Zygote,12(4):333-338 2004(Nov.)
Author:H. Iwayama, S. Hochi, M. Kato, M. Hirabayashi, M. Kuwayama, H. Ishikawa, S. Ohsumi, Y. Fukui

【論60】 Acquirement of oocyte activating factor in Antarctic minke whale (Balaenopterabonaerensis) spermatogenic cells, assessed by meiosis resumption of microinseminated mouse oocytes
J. Mammal. Ova Res.,21(3):149-156 2004(Oct.)
Author:K. Amemiya, Y. Iwanami, T. Kobayashi, T. Terao, Y. Fukui, H. Ishikawa, S. Ohsumi, M. Hirabayashi, S. Hochi

【論59】 Production of transgenic rats by ooplasmic injection of spermatogenic cells exposed to exogenous DNA: A preliminary study
Mol. Reprod. Dev.,69(2):153-158 2004(Oct.)
Author:M. Kato, A. Ishikawa, R. Kaneko, T. Yagi, S. Hochi, M. Hirabayashi

【論58】 Donor and recipient rat strains affect full-term development of one-cell zygotes cultured to morulae/blastocysts
J. Reprod. Dev.,50(2):191-195 2004(Apr.)
Author:M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi

【論57】 Effect of activation regimens for rat oocytes on full-term development after round spermatid injection
Contem. Top. Lab. Anim. Sci.,43(2):13-15 2004(Mar.)
Author:M. Kato, A. Ishikawa, S. Hochi, M. Hirabayashi

【論56】 Successful vitrification of pronuclear-stage rabbit zygotes by minimum volume cooling procedure
Theriogenology,61(2-3):267-275 2004(Jan.)
Author:S. Hochi, T. Terao, M. Kamei, M. Kato, M. Hirabayashi, M. Hirao

【論55】 Factors affecting premature chromosome condensation of cumulus cell nuclei injected into rat oocytes
J. Reprod. Dev.,49(2):121-126 2003(Apr.)
Author:M. Hirabayashi, M. Kato, A. Takeuchi, A. Ishikawa, S. Hochi

【論54】 Factors influencing chromosome condensation and development of cloned rat embryos
Cloning Stem Cells,5(1):35-42 2003
Author:M. Hirabayashi, M. Kato, A. Ishikawa, S. Hochi

【論53】 Rescue of infertile transgenic rat lines by intracytoplasmic injection of cryopreserved round spermatids
Mol. Reprod. Dev.,62(3):295-299 2002(Jul.)
Author:M. Hirabayashi, M. Kato, T. Aoto, M. Ueda, S. Hochi

【論52】 Effect of Ca(2+)/Mg(2+)-free culture condition on spontaneous first cleavage of rat oocytes
J. Reprod. Dev.,48(3):243-248 2002(Jun.)
Author:A. Takeuchi, M. Kato, K. Ito, K. Kimura, A. Hanada, M. Hirabayashi, S. Hochi

【論51】 Offspring derived from intracytoplasmic injection of transgenicrat sperm
Transgenic Res.,11(2):221-228 2002(Apr.)
Author:M. Hirabayashi, M. Kato, T. Aoto, A. Sekimoto, M. Ueda, I. Miyoshi, N. Kasai, S. Hochi

【論50】 Strontium-induced activation regimen for rat oocytes in somatic cell nuclear transplantation
J. Reprod. Dev.,47(6):407-413 2001(Dec.)
Author:M. Kato, M. Hirabayashi, T. Aoto, K. Ito, M. Ueda, S. Hochi

【論49】 Production of transgenic rats using young Sprague-Dawley females treated with PMSG and hCG
Exp. Anim.,50(5):365-369 2001(Oct.)
Author:M. Hirabayashi, K. Ito, A. Sekimoto, S. Hochi, M. Ueda

【論48】 Effects of cryopreservation of pronuclear-stage rabbit zygotes on the morphological survival, blastocyst formation, and full-term development after DNA microinjection
Mol. Reprod. Dev.,60(2):227-232 2001(Oct.)
Author:S. Hochi, M. Hirabayashi, M. Hirao, M. Kato, T. Kobayashi, K. Kimura, K. Hirasawa, S.P. Leibo, M. Ueda

【論47】 A comparative study on the integration of exogenous DNA into mouse, rat, rabbit, and pig genomes
Exp. Anim.,50(2):125-131 2001(Apr.)
Author:M. Hirabayashi, R. Takahashi, K. Ito, N. Kashiwazaki, M. Hirao, K. Mirasawa, S. Hochi, M. Ueda

【論46】 Effects of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes
Cryobiology,42(1):69-73 2001(Feb.)
Author:S. Hochi, M. Akiyama, G. Minagawa, K. Kimura, A. Hanada

【論44】 Production of transgenic rabbits using centrifuged pronuclear zygotes
J. Vet. Med. Sci.,62(10):1047-1052 2000(Oct.)
Author:M. Hirabayashi, M. Hirao, R. Takahashi, K. Kimura, K. Hirasawa, M. Ueda, S. Hochi

【論43】 Nuclear transfer in cattle: effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes
J. Vet. Med. Sci.,62(10):1111-1113 2000(Oct.)
Author:S. Hochi, M. Kato, K. Ito, M. Hirabayashi, M. Ueda, A. Sekimoto, Y. Nagao, K. Kimura, A. Hanada

【論41】 Effect of time interval between biopsy and vitrification on survival of in vitro-produced bovine blastocysts
J. Reprod. Dev.,45(5):351-355 1999(Oct.)
Author:K. Ito, A. Sekimoto, M. Hirabayashi, S. Hochi, K. Kimura, M. Ueda, Y. Nagao

【論40】 Effects of timing of oocyte cryopreservation on in vitro development of nuclear-transferred bovine zygotes
Mol. Reprod. Dev.,54(1):81-85 1999(Sep.)
Author:K. Ito, M. Hirabayashi, M. Ueda, Y. Nagao, K. Kimura, A. Hanada, S. Hochi

【論39】 Effect of linoleic acid-albumin in the culture medium on freezing sensitivity of in vitro-produced bovine morulae
Theriogenology,52(3):497-504 1999(Aug.)
Author:S. Hochi, K. Kimura, A. Hanada

【論38】 Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes
J. Mammal. Ova Res.,16(1):19-22 1999(Apr.)
Author:S. Hochi, A. Kanamori, K. Sugisawa, K. Kimura, A. Hanada

【論37】 Effects of percoll washing and glycosaminoglycans on the lysophosphatidylcholin induced acrosomal reaction in stallion
J. Equine Sci.,9(4):101-106 1998(Dec.)
Author:M. Kobayashi, M.P.B. Wijayagunawardane, Y.H. Choi, A.Y. Kojima, S. Hochi, N. Oguri, M. Takagi, K. Miyazawa, K. Sato

【論36】 Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage
Theriogenology,50(7):1093-1100 1998(Nov.)
Author:K. Ito, S. Otake, M. Hirabayashi, S. Hochi, M. Ueda

【論35】 Effect of nuclear stages during IVM on the survival of vitrified-warmed bovine oocytes
Theriogenology,49(4):787-796 1998(Mar.)
Author:S. Hochi, K. Ito, M. Hirabayashi, M. Ueda, K. Kimura, A. Hanada

【論33】 In vitro fertilizing ability of bovine oocytes frozen-thawed at immature, maturing, and mature stages
J. Mammal. Ova Res.,14(1):61-65 1997(Apr.)
Author:S. Hochi, A. Kanamori, K. Kimura, A. Hanada

【論32】 Direct transfer of equine blastocysts frozen-thawed in the presence of ethylene glycol and sucrose
Theriogenology,46(7):1217-1224 1996(Nov.)
Author:S. Hochi, K. Maruyama, N. Oguri

【論31】 Effect of cooling and warming rates during cryopreservation on survival of in vitro-produced bovine embryos
Theriogenology,46(5):837-847 1996(Oct.)
Author:S. Hochi, E. Semple, S.P. Leibo

【論30】 In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification
Cryobiology,33(3):300-310 1996(Jun.)
Author:S. Hochi, M. Kozawa, T. Fujimoto, E. Hondo, J. Yamada, N. Oguri

【論29】 In vitro capacitation of stallion spermatozoa assessed by the lysophoshatidylcholine-induced acrosome reaction and the penetration rate into in vitro-matured, zona-free mare oocytes
J. Equine Vet. Sci.,16(6):244-248 1996(Jun.)
Author:S. Hochi, K. Korosue, Y.H. Choi, N. Oguri

【論28】 Activation of glutathione transferase P gene by lead requires glutathione transferase P enhancer I
J. Biol. Chem.,271(3):1626-1632 1996(Jan.)
Author:T. Suzuki, S. Morimura, M.B. Diccianni, R. Yamada, S. Hochi, M. Hirabayashi, A. Yuki, K. Nomura, T. Kitagawa, M. Imagawa, M. Muramatsu

【論27】 Effect of different protein supplements on motility and plasma membrane integrity of frozen-thawed stallion spermatozoa
Cryobiology,32(5):487-492 1995(Oct.)
Author:J. Braun, S. Hochi, N. Oguri, K. Sato, F. Torres-Boggino

【論26】 Effect of glucose in the culture medium on development of horse oocytes matured and microfertilized in vitro
Reprod. Fertil. Dev.,7(5):1067-1071 1995(Oct.)
Author:T. Azuma, Y.H. Choi, S. Hochi, N. Oguri

【論25】 Tissue specific activation of tumor marker glutathione transferase P transgenes in transgenic rats
J. Can. Res. Clin. Oncol.,121(9-10):606-611 1995(Sep.)
Author:T. Suzuki, M. Imagawa, K. Nomura, S. Hochi, M. Hirabayashi, M. Ueda, T. Kitagawa, M. Muramatsu

【論24】 An attempt to produce acellular embryonic capsule in equine blastocysts cultured in vitro
J. Reprod. Dev.,41(4):367-371 1995(Aug.)
Author:S. Hochi, T. Azuma, K. Miyaushiro, N. Oguri

【論23】 Relationship among seminal characteristics, fertility, and suitability for semen preservation in Draft stallions
J. Vet. Med. Sci.,57(2):225-229 1995(Apr.)
Author:F. Torres-Boggino, K. Sato, A. Oka, Y. Kanno, S. Hochi, N. Oguri, J. Braun

【論22】 Large equine blastocysts are damaged by vitrification procedures
Reprod. Fertil. Dev.,7(1):113-117 1995(Feb.)
Author:S. Hochi, T. Fujimoto, N. Oguri

【論21】 Cryopreservation of equine oocytes by 2-step freezing
Theriogenology,42(7):1085-1094 1994(Nov.)
Author:S. Hochi, T. Fujimoto, Y.H. Choi, J. Braun, N. Oguri

【論20】 In vitro fertilization rate of horse oocytes with partially removed zonae
Theriogenology,42(5):795-802 1994(Oct.)
Author:Y.H. Choi, Y. Okada, S. Hochi, J. Braun, K. Sato,N. Oguri

【論18】 Effects of individual stallions and mare breeds on assisted fertilization in vitro
J. Equine Sci.,5(3):77-81 1994(Sep.)
Author:S. Hochi, Y.H. Choi, K. Korosue, K. Sato, N. Oguri

【論17】 Pregnancies following transfer of equine embryos cryopreserved by vitrification
Theriogenology,42(3):483-488 1994(Aug.)
Author:S. Hochi, T. Fujimoto, J. Braun, N. Oguri

【論16】 Effect of seminal plasma on motion characteristics of epididymal and ejaculated stallion spermatozoa during storage at 5˚C
Deutsche Tierärztliche Wochenschrift,101(8):319-322 1994(Aug.)
Author:J. Braun, F. Torres-Boggino, S. Hochi, N. Oguri

【論15】 Influence of relative embryonic volumes during glycerol equilibration on the survival of frozen-thawed equine blastocysts
J. Reprod. Dev.,40(3):243-249 1994(Jun.)
Author:S. Hochi, M. Ogasawara, J. Braun, N. Oguri

【論14】 Follicular development, progesterone and estradiol 17-β levels during spontaneous and FSH-stimulated estrous cycles in Hokkaido Native pony and Thoroughbred mares
J. Reprod. Dev.,40(2):141-147 1994(Apr.)
Author:J. Braun, Y. Moriuchi, S. Nakagawa, S. Hochi, A. Miyamoto, N. Oguri

【論13】 Preservation of ejaculated and epididymal stallion spermatozoa by cooling and freezing
Theriogenology,41(4):809-818 1994(Mar.)
Author:J. Braun, M. Sakai, S. Hochi, N. Oguri

【論12】 Influence of sera on in vitro hatching of equine blastocysts
J. Reprod. Dev.,40(1):13-18 1994(Feb.)
Author:S. Hochi, J. Braun, A. Miyamoto, Y. Fukui, N. Oguri

【論9】 In vitro maturation of equine oocytes collected by follicle aspiration and by the slicing of ovaries
Theriogenology,40(5):959-966 1993(Nov.)
Author:Y.H. Choi, S. Hochi, J. Braun, K. Sato, N. Oguri

【論8】 The hatching process of equine embryos in vitro
Equine Vet. J. Suppl.,15:91-94 1993(Oct.)
Author:S. Hochi, J. Braun, N. Oguri

【論7】 Trans-activation of glutathione transferase P gene during chemical hepatocarcinogenesis of the rat
Proc. Natl. Acad. Sci. USA,90(5):2065-2068 1993(Mar.)
Author:S. Morimura, T. Suzuki, S. Hochi, A. Yuki, K. Nomura, T. Kitagawa, I. Nagatsu, M. Imagawa, M. Muramatsu

【論6】 Secretion of bovine α-lactalbumin into the milk of transgenic rats
Mol. Reprod. Dev.,33(2):160-164 1992(Oct.)
Author:S. Hochi, T. Ninomiya, M. Waga-Honma, J. Sagara, A. Yuki

【論5】 Cryoprotective effect of polyols on rat embryos during two-step freezing
Cryobiology,29(3):332-341 1992(Jun.)
Author:K. Utsumi, S. Hochi, A. Iritani

【論4】 Successful production of transgenic rats
Anim. Biotechnol.,1(2):175-184 1990(Dec.)
Author:S. Hochi, T. Ninomiya, M. Honma, A. Yuki

【論3】 DNA breaks and repair in the mouse leukemia L1210 cells exposed to three types of interstrand DNA cross-linkers
Mutat. Res.,236(1):43-50 1990(Jul.)
Author:N. Yajima, S. Hochi, N. Miyata, T. Kishi, G. Kawanishi

【論2】 Fate of exogenous DNA carried into mouse eggs by spermatozoa
Anim. Biotechnol.,1(1):21-31 1990(Jun.)
Author:S. Hochi, T. Ninomiya, A. Mizuno, M. Honma, A. Yuki

Cryodevices developed for minimum volume cooling vitrification of bovine oocytes
Anim. Sci. J.,93(1):e13683 2022(Jan.)
Author:S. Hochi

【総説16】 Pluripotent stem cell-derived organogenesis in the rat model system
Transgenic Res.,28(3-4):287-297 2019(Aug.)
Author:M. Hirabayashi, T. Goto, S. Hochi

【総説15】 Microtubule assembly crucial to bovine embryonic development in assisted reproductive technologies
Anim. Sci. J.,87(9):1076-1083 2016(Sep.)
Author:S. Hochi

【総説14】 Recent progress in cryopreservation of bovine oocytes
BioMed. Res. Intern.,2014:570647 2014(Mar.)
Author:I-S. Hwang, S. Hochi

【総説13】 Challenging endeavour for preservation of freeze-dried mammalian spermatozoa
J. Reprod. Dev.,57(5):557-563 2011(Oct.)
Author:S. Hochi, H. Abdalla, H. Hara, M. Hirabayashi

【総説12】 Embryonic stem (ES) cells and induced pluripotent stem (iPS) cells in rats
Reprod. Med. Biol.,10(4):231-238 2011(Jun.)
Author:M. Hirabayashi, S. Hochi

【総説11】 Active demethylation of paternal genome in mammalian zygotes
J. Reprod. Dev.,55(4):356-360 2009(Aug.)
Author:H, Abdalla, Y. Yoshizawa, S. Hochi

【総説9】 Transgenesis via intracytoplasmic sperm injection (ICSI) in rodents
J. Mammal. Ova Res.,23(3):86-90 2006(Oct.)
Author:M. Hirabayashi, M. Kato, S. Hochi

【総説6】 Cryopreservation of follicular oocytes and preimplantation embryos in cattle and horses
J. Reprod. Dev.,49(1):13-21 2003(Feb.)
Author:S. Hochi

【総説5】 Maintenance of transgenes in rats: the contributions of embryo cryopreservation and intracytoplasmic sperm/spermatid injection
J. Reprod. Dev.,48(3):205-213 2002(Jun.)
Author:S. Hochi, M. Hirabayashi

【総説2】 Current status of IVM/IVF/IVC technology and embryo cryopreservation in the equine species
J. Mammal. Ova Res.,15(3):117-127 1998(Oct.)
Author:S. Hochi

Research Grants
Grants‐in‐aid for Scientific Research(Researcher)
2021 - 2024 , 超瞬間レーザー解凍による次世代細胞凍結保存技術の確立とバイオバンキングへの展開 , 基盤研究
Research Activities
Journal Editorial Board
2008-2022 , J. Reprod. Dev. (IPEC Inc.)
2008-2022 , Theriogenology (Elsevier Inc.)

Journal Reviewer
1998-2022 , Theriogenology (65) / Reprod. Domest. Anim. (20) / J. Reprod. Dev. (14) / Cryobiology (14) / J. Assist. Reprod. Genet. (10) / Reproduction (5) / Zygote (5) 他. 計37誌 215編


2022 , Cell Technology
2022 , Scientific English
2022 , Safety Education
2022 , Invitation to Applied Biology
2022 , Biology World
2022 , Topic in Animal Reproductive Technology (Doctor course)
2022 , Topic in Animal Science (Master course)
2022 , Animal Physiology