Takeno, Seiki | Shinshu University Researcher Directory

Academic Organization	Academic Assembly　School of Science and Technology　Institute of Agriculture	TEL
Education and Research Organization	Department of Agricultural and Life Sciences, Faculty of Agriculture	FAX
Position	Associate Professor	Mail Address
Address	Department of Agricultural and Life Sciences, Faculty of Agriculture, Shinshu University, Nagano 399-4598, Japan	Web site	http://www.shinshu-u.ac.jp/faculty/agriculture/lab/ferment/

Profile

Research Field: Applied Microbiology

Current Subject: Metabolic engineering and molecular breeding of amino acid-producing bacteria

Academic Societies: Academic Societies
Japan Society for Bioscience, Biotechnology, and Agrochemistry
The Society for Biotechnology, Japan

Academic Background: Graduate School
Kyoto University , (Division of Applied Life Sciences , Graduate School of Agriculture) , 2004
Yamaguchi University , (生物資源科学専攻 , Graduate School of Agriculture) , 2001

College
Yamaguchi University , (Faculty of Agriculture) , 1999

Degree
Ph. D. , 京都大学（日本）

Awards: 2021 , Spotlight article【米国微生物学会誌（Appl Environ Microbiol）】
2020 , 日本農芸化学会2020年度大会トピックス演題選出(1598演題中の31演題）

Research Career: Research Career
2015- , 信州大学学術研究院農学系　准教授
2014-2015 , 信州大学学術研究院農学系　助教
2007-2014 , Assistant Professor, Shinshu University
2005-2007 , AssistantProfessor, Shinshu University
2005-2005 , 京都大学　産学官連携研究員
2004-2005 , 京都大学　産学連携研究員
2004-2004 , 京都大学　教務補佐員

Research

Books, Articles, etc.: Books
Recent Advances in Amino Acid Production, Corynebacterium glutamicum -Biology and Biotechnology-, 2nd Edition, Microbiology Monographs 23 , 175-226
Springer 2020(Apr.)
Author:Ikeda M, Takeno S

Transformation of Zygomycete Mortierella alpina Using Biolistic Particle Bombardment, Genetic Transformation Systems in Fungi, Volume 1 , 135-140
Springer 2014
Author:Sakuradani E, Kikukawa H, Takeno S, Ando A, Shimizu S, Ogawa J.

Amino Acid Production by Corynebacterium glutamicum, Corynebacterium glutamicum -Biology and Biotechnology-, Microbiology Monographs 23 , 107-147
Springer 2013
Author:Ikeda M, Takeno S.

Genetic Engineering of Corynebacteria, Manual of Industrial Microbiology and Biotechnology, 3rd Edition , 225-237
2010
Author:Ikeda M, Takeno S.

Arachidonic Acid-Producing Mortierella alpina: Molecular Breeding of Mutants and Creation and Application of a Host-Vector System, Biocatalysis and Biotechnology for Functional Foods and Industrial Products , 267-282
2006
Author:Sakuradani E, Takeno S, Abe T, Ogawa J, Shimizu S.

Arachidonic Acid-Producing Mortierella alpina: Creation of Mutants and Molecular Breeding., Single Cell Oils , 21-35
2005
Author:Sakuradani E, Takeno S, Abe T, Shimizu S.

Articles
Metabolic engineering to produce palmitic acid or palmitoleic acid in an oleic acid-producing Corynebacterium glutamicum strain
Metab. Eng.,78:148-158 2023(Jul.)
Author:Takeno S, Hirata Y, Kitamura K, Ohtake T, Aoki K, Murata N, Hayashi M, Ikeda M.

A Futile Metabolic Cycle of Fatty Acyl Coenzyme A (Acyl-CoA) Hydrolysis and Resynthesis in Corynebacterium glutamicum and Its Disruption Leading to Fatty Acid Production
Appl. Environ. Microbiol.,87(4):e02469-20 2021(Feb.)
Author:Ikeda M, Takahashi K, Ohtake T, Imoto R, Kawakami H, Hayashi M, Takeno S.

The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains
Appl. Microbiol. Biotechnol.,102(24):10603-10612 2018(Dec.)
Author:Takeno S, Murata N, Kura M, Takasaki M, Hayashi M, Ikeda M.

In vivo roles of fatty acid biosynthesis enzymes in biosynthesis of biotin and α-lipoic acid in Corynebacterium glutamicum
Appl. Environ. Microbiol.,83(19):e01322-17 2017(Oct.)
Author:Ikeda M, Nagashima T, Nakamura E, Kato R, Ohshita M, Hayashi M, Takeno S.

L-Lysine production independent of the oxidative pentose phosphate pathway by Corynebacterium glutamicum with the Streptococcus mutans gapN gene
Metab. Eng.,37:1-10 2016
Author:Takeno S, Hori K, Ohtani S, Mimura A, Mitsuhashi S, Ikeda M.

A third glucose uptake bypass in Corynebacterium glutamicum ATCC 31833
Appl. Microbiol. Biotechnol.,99:2741-2750 2015
Author:Ikeda M, Noguchi N, Ohshita M, Senoo A, Mitsuhashi S, Takeno S.

Development of Fatty Acid-Producing Corynebacterium glutamicum Strains
Appl. Environ. Microbiol.,79:6776-6783 2013
Author:Takeno S, Takasaki M, Urabayashi A, Mimura A, Muramatsu T, Mitsuhashi S, Ikeda M.

Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains
Appl. Environ. Microbiol.,79:4586-4594 2013
Author:Ikeda M, Miyamoto A, Mutoh S, Kitano Y, Tajima M, Shirakura D, Takasaki M, Mitsuhashi S, Takeno S.

Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum
Appl. Microbiol. Biotechnol.,97:1259-1267 2013
Author:Takeno S, Shirakura D, Tsukamoto N, Mitsuhashi S, Ikeda M.

Identification and application of a different glucose uptake system that functions as an alternative to the phosphotransferase system in Corynebacterium glutamicum
Appl. Microbiol. Biotechnol.,90:1443-1451 2011
Author:Ikeda M, Mizuno Y, Awane S, Hayashi M, Mitsuhashi S, Takeno S.

Engineering of Corynebacterium glutamicum with an NADPH-generating glycolytic pathway for L-lysine production
Appl. Environ. Microbiol.,76:7154-7160 2010
Author:Takeno S, Murata R, Kobayashi R, Mitsuhashi S, Ikeda M.

Elucidation of Genes Relevant to the Microaerobic Growth of Corynebacterium glutamicum
Biosci. Biotechnol. Biochem.,73:2806-2808 2009
Author:Ikeda M, Baba M, Tsukamoto N, Komatsu T, Mitsuhashi S, Takeno S.

The Cgl1281-encoding putative transporter of the cation diffusion facilitator family is responsible foralkali-tolerance in Corynebacterium glutamicum
Arch. Microbiol.,190:531-538 2008
Author:Takeno S, Nakamura M, Fukai R, Ohnishi J, Ikeda M.

Characterization of mutations induced by N-methyl-N'-nitro-N-nitrosoguanidine in an industrial Corynebacterium glutamicum strain
Mutat. Res.,649:239-244 2008
Author:Ohnishi J, Mizoguchi H, Takeno S, Ikeda M.

Anaerobic growth and potential for amino acid production by nitrate respiration in Corynebacterium glutamicum
Appl. Microbiol. Biotechnol.,75:1173-1182 2007
Author:Takeno S, Ohnishi J, Komatsu T, Masaki T, Sen K, Ikeda M.

Transformation of Oil-Producing Fungus, Mortierella alpina 1S-4, Using Zeocin, and Application to Arachidonic Acid Production
J. Biosci. Bioeng.,100:617-622 2005
Author:Takeno S, Sakuradani E, Tomi A, Inohara-Ochiai M, Kawashima H, Shimizu S.

Improvement of the Fatty Acid Composition of an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4, through RNA Interference with delta12-Desaturase Gene Expression
Appl. Environ. Microbiol.,71:5124-5128 2005
Author:Takeno S, Sakuradani E, Tomi A, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S.

Molecular Evidence That the Rate-Limiting Step for the Biosynthesis of Arachidonic Acid in Mortierella alpina Is at the Level of an Elongase
Lipids,40:25-30 2005
Author:Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S.

Establishment of an Overall TransformationSystem for an Oil-Producing Filamentous Fungus, Mortierella alpina 1S-4
Appl. Microbiol. Biotechnol.,65:419-425 2004
Author:Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S.

Cloning and Sequencing of the ura3 and ura5 Genes, and Isolation and Characterization of Uracil Auxotrophs of the Fungus Mortierella alpina 1S-4
Biosci. Biotechnol. Biochem.,68:277-285 2004
Author:Takeno S, Sakuradani E, Murata S, Inohara-Ochiai M, Kawashima H, Ashikari T, Shimizu S.

Enzymes Responsible for Acetate Oxidation by Acetic Acid Bacteria
Biosci. Biotechnol. Biochem.,63:2102-2109 1999
Author:Saeki A, Matsushita K, Takeno S, Taniguchi M, Toyama H, Theeragool G, Lotong N, Adachi O.

Presentations
コリネ型アミノ酸生産菌における還元力（NADPH）産生の代謝工学
日本農芸化学会2011年度大会シンポジウム（講演番号 4SY22-2） 2011(Mar. 28)
Presenter:竹野誠記，村田了祐，妹尾彰宏，三橋敏，池田正人

アミノ酸生産菌を用いた脂質発酵へのアプローチ
日本農芸化学会中部支部第157回例会若手シンポジウム 2009(Nov. 14)
Presenter:竹野誠記

MISC
ミュータンス菌型糖代謝による新規発酵技術
ケミカルエンジニヤリング,62(9):625-633 2017(Sep. 01)
Author:竹野誠記，池田正人

シクロプロパン脂肪酸の役割
生物工学会誌,85:83 2007
Author: 竹野誠記

油脂をつくる微生物の探索と育種　－最近の進歩－
学術月報,58:757-761 2005
Author: 竹野誠記，櫻谷英治、清水昌

油糧微生物Mortierella aplina1S-4における宿主-ベクター系の開発とその応用
応用微生物学研究,2:82-87 2004
Author: 竹野誠記，櫻谷英治、清水昌

Patents: Patents
有用物質の製造法，特許5897197（2016.3.11登録）
有用物質の製造法，特許5833311（2015.11.6登録）
有用物質の製造方法，特許5537818 （2014.5.9登録）
Process for producing useful substance，US8530203 （2013.9.10登録）

Research Grants: Grants‐in‐aid for Scientific Research(Research Representative)
2022 - 2024 , 鉄の獲得に着目したパルミトレイン酸生産菌の改良 , 基盤研究（C）
2019 - 2021 , パルミトレイン酸生産菌の開発 , 基盤研究（C）
2014 - 2016 , コリネ型細菌における長鎖脂肪酸排出輸送体の同定 , 若手研究（B）

Shinshu University Researcher Directory

Takeno Seiki

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Research

Takeno　Seiki